WO2001025420A3 - Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis - Google Patents

Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis Download PDF

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Publication number
WO2001025420A3
WO2001025420A3 PCT/US2000/027677 US0027677W WO0125420A3 WO 2001025420 A3 WO2001025420 A3 WO 2001025420A3 US 0027677 W US0027677 W US 0027677W WO 0125420 A3 WO0125420 A3 WO 0125420A3
Authority
WO
WIPO (PCT)
Prior art keywords
interaction systems
protein
analysis
detection
adjustable sensitivity
Prior art date
Application number
PCT/US2000/027677
Other languages
French (fr)
Other versions
WO2001025420A2 (en
Inventor
David N Edwards
Arlene Leon
David F Ranney
Original Assignee
Hybrigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybrigen Inc filed Critical Hybrigen Inc
Priority to AU10744/01A priority Critical patent/AU1074401A/en
Priority to CA002386258A priority patent/CA2386258A1/en
Priority to IL14896700A priority patent/IL148967A0/en
Priority to JP2001528573A priority patent/JP2004500805A/en
Priority to EP00972024A priority patent/EP1222261A2/en
Publication of WO2001025420A2 publication Critical patent/WO2001025420A2/en
Publication of WO2001025420A3 publication Critical patent/WO2001025420A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

A method for detecting interactions between first and second interacting molecules at variable sensitivity. This variable sensitivity may be obtained by providing for the overexpression of either a bait hybrid protein containing a DNA binding domain (desensitization) or a prey hybrid protein containing the DNA activation domain for a reporter gene (enhanced sensitivity). The use of exogenous activators of one or the other according to the needs of a particular system is readily accomplished.
PCT/US2000/027677 1999-10-07 2000-10-06 Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis WO2001025420A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU10744/01A AU1074401A (en) 1999-10-07 2000-10-06 Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis
CA002386258A CA2386258A1 (en) 1999-10-07 2000-10-06 Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis
IL14896700A IL148967A0 (en) 1999-10-07 2000-10-06 Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis
JP2001528573A JP2004500805A (en) 1999-10-07 2000-10-06 Tunable sensitivity genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis
EP00972024A EP1222261A2 (en) 1999-10-07 2000-10-06 Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15807999P 1999-10-07 1999-10-07
US60/158,079 1999-10-07

Publications (2)

Publication Number Publication Date
WO2001025420A2 WO2001025420A2 (en) 2001-04-12
WO2001025420A3 true WO2001025420A3 (en) 2002-04-18

Family

ID=22566603

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/027677 WO2001025420A2 (en) 1999-10-07 2000-10-06 Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis

Country Status (6)

Country Link
EP (1) EP1222261A2 (en)
JP (1) JP2004500805A (en)
AU (1) AU1074401A (en)
CA (1) CA2386258A1 (en)
IL (1) IL148967A0 (en)
WO (1) WO2001025420A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE540115T1 (en) * 2007-07-11 2012-01-15 Yeda Res & Dev NUCLEIC ACID CONSTRUCT SYSTEMS WITH CAPABILITY TO DIAGNOSE OR TREAT A CELL CONDITION
AU2017271678B2 (en) 2016-05-27 2021-05-06 Synthex, Inc. Protein interfaces
GB2584210B (en) 2017-11-22 2022-03-16 Synthex Inc Peptides for inhibiting RAD51

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997031113A1 (en) * 1996-02-23 1997-08-28 Ariad Pharmaceuticals, Inc. Cell-based assay
US5925523A (en) * 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997031113A1 (en) * 1996-02-23 1997-08-28 Ariad Pharmaceuticals, Inc. Cell-based assay
US5925523A (en) * 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FINLEY R L ET AL: "TWO-HYBRID ANALYSIS OF GENETIC REGULATORY NETWORKS. THE YEAST TWO-HYBRID SYSTEM", YEAST TWO-HYBRID SYSTEM ( ADVANCES IN MOLECULAR BIOLOGY ), XX, XX, 1997, pages 197 - 214, XP002922642 *

Also Published As

Publication number Publication date
EP1222261A2 (en) 2002-07-17
JP2004500805A (en) 2004-01-15
WO2001025420A2 (en) 2001-04-12
IL148967A0 (en) 2002-11-10
AU1074401A (en) 2001-05-10
CA2386258A1 (en) 2001-04-12

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