WO2001024937A3 - Separation apparatus and method - Google Patents

Separation apparatus and method Download PDF

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Publication number
WO2001024937A3
WO2001024937A3 PCT/GB2000/003763 GB0003763W WO0124937A3 WO 2001024937 A3 WO2001024937 A3 WO 2001024937A3 GB 0003763 W GB0003763 W GB 0003763W WO 0124937 A3 WO0124937 A3 WO 0124937A3
Authority
WO
WIPO (PCT)
Prior art keywords
magnetic
dsdna
magnetisable
sleeves
members
Prior art date
Application number
PCT/GB2000/003763
Other languages
French (fr)
Other versions
WO2001024937A2 (en
Inventor
Peter Hagerlid
Mathias Uhlen
Original Assignee
Pyrosequencing Ab
Piesold Alexander James
Peter Hagerlid
Mathias Uhlen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pyrosequencing Ab, Piesold Alexander James, Peter Hagerlid, Mathias Uhlen filed Critical Pyrosequencing Ab
Priority to AU75396/00A priority Critical patent/AU7539600A/en
Publication of WO2001024937A2 publication Critical patent/WO2001024937A2/en
Publication of WO2001024937A3 publication Critical patent/WO2001024937A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/284—Magnetic plugs and dipsticks with associated cleaning means, e.g. retractable non-magnetic sleeve
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A separation device is disclosed comprising an array of magnetic or magnetisable members (2) and a corresponding array of protective sleeves (6) therefor associated with the magnetic members (2). The magnetic members (2) are removable from the sleeves (6). The sleeves (6) in turn may be removably introduced into the wells (5) of a micro-titre plate (4). Also disclosed is a method of preparing single-stranded DNA (ssDNA) from double-stranded DNA (dsDNA) comprising the steps of immobilising a strand of the dsDNA on a solid magnetic or magnetisable carrier, inserting a magnetic or magnetisable member into a solution containing the DNA to attract said carrier, separating the dsDNA into single strands, and thereafter removing the member from the solution thereby removing ssDNA from the solution.
PCT/GB2000/003763 1999-10-01 2000-10-02 Separation apparatus and method WO2001024937A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU75396/00A AU7539600A (en) 1999-10-01 2000-10-02 Separation apparatus and method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9923324.9 1999-10-01
GBGB9923324.9A GB9923324D0 (en) 1999-10-01 1999-10-01 Separation apparatus and method

Publications (2)

Publication Number Publication Date
WO2001024937A2 WO2001024937A2 (en) 2001-04-12
WO2001024937A3 true WO2001024937A3 (en) 2001-08-30

Family

ID=10862000

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/003763 WO2001024937A2 (en) 1999-10-01 2000-10-02 Separation apparatus and method

Country Status (3)

Country Link
AU (1) AU7539600A (en)
GB (1) GB9923324D0 (en)
WO (1) WO2001024937A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
EP1343973B2 (en) 2000-11-16 2020-09-16 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn Nucleic acid amplification utilizing microfluidic devices
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
ES2403560T3 (en) 2001-11-30 2013-05-20 Fluidigm Corporation Microfluidic device and procedures for its use
EP1474511B1 (en) * 2002-02-12 2005-04-27 Biotage AB Separating method and an apparatus performing such a method
WO2003085379A2 (en) 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
EP2298448A3 (en) 2002-09-25 2012-05-30 California Institute of Technology Microfluidic large scale integration
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
KR100483684B1 (en) * 2003-01-29 2005-04-18 (주)바이오넥스 Kit for separating and purifying nucleic acids or various biological materials, and system for automatically performing separation or purification of biological materials using the same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
DE602005020421D1 (en) 2004-02-19 2010-05-20 Helicos Biosciences Corp METHOD FOR THE ANALYSIS OF POLYNUCLEOTIDE SEQUENCES
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7815868B1 (en) 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58143854A (en) * 1982-02-18 1983-08-26 Nec Corp Apparatus for recovering magnetic material
WO1999042832A1 (en) * 1998-02-23 1999-08-26 Bio-Nobile Oy Magnetic particle transfer device and method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58143854A (en) * 1982-02-18 1983-08-26 Nec Corp Apparatus for recovering magnetic material
WO1999042832A1 (en) * 1998-02-23 1999-08-26 Bio-Nobile Oy Magnetic particle transfer device and method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 7, no. 259 (C - 195)<1404> 18 November 1983 (1983-11-18) *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules

Also Published As

Publication number Publication date
GB9923324D0 (en) 1999-12-08
WO2001024937A2 (en) 2001-04-12
AU7539600A (en) 2001-05-10

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