WO2001019962A3 - Method of preserving tissue viability during mechanical separation process - Google Patents

Method of preserving tissue viability during mechanical separation process Download PDF

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Publication number
WO2001019962A3
WO2001019962A3 PCT/US2000/025372 US0025372W WO0119962A3 WO 2001019962 A3 WO2001019962 A3 WO 2001019962A3 US 0025372 W US0025372 W US 0025372W WO 0119962 A3 WO0119962 A3 WO 0119962A3
Authority
WO
WIPO (PCT)
Prior art keywords
tissue
mechanical separation
viability during
separation process
units
Prior art date
Application number
PCT/US2000/025372
Other languages
French (fr)
Other versions
WO2001019962A2 (en
Inventor
Anton-Lewis Usala
Richard Chris Klann
Original Assignee
Encelle Inc
Usala Anton Lewis
Richard Chris Klann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Encelle Inc, Usala Anton Lewis, Richard Chris Klann filed Critical Encelle Inc
Priority to AU73823/00A priority Critical patent/AU7382300A/en
Publication of WO2001019962A2 publication Critical patent/WO2001019962A2/en
Publication of WO2001019962A3 publication Critical patent/WO2001019962A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Obesity (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Emergency Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a method of preserving tissue viability during separation, the method including placing intact tissue in a cell culture medium. The cell culture medium includes at least one nitric oxide inhibitor. The tissue is mechanically separated into a plurality of physiologic tissue units, each unit maintaining physiologic cell-to-cell connections within the unit. The mechanical separation step may comprise cutting the tissue into slices and tearing the slices of tissue into a plurality of tissue units. Thereafter, the tissue units may be embedded in a hydrogel matrix for storage.
PCT/US2000/025372 1999-09-15 2000-09-15 Method of preserving tissue viability during mechanical separation process WO2001019962A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU73823/00A AU7382300A (en) 1999-09-15 2000-09-15 Method of preserving tissue viability during mechanical separation process

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39687599A 1999-09-15 1999-09-15
US09/396,875 1999-09-15

Publications (2)

Publication Number Publication Date
WO2001019962A2 WO2001019962A2 (en) 2001-03-22
WO2001019962A3 true WO2001019962A3 (en) 2001-10-18

Family

ID=23568967

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/025372 WO2001019962A2 (en) 1999-09-15 2000-09-15 Method of preserving tissue viability during mechanical separation process

Country Status (2)

Country Link
AU (1) AU7382300A (en)
WO (1) WO2001019962A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102432491B (en) * 2011-09-08 2013-10-23 西安科技大学 Gelling agent based on tyrosine derivative and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834005A (en) * 1992-02-24 1998-11-10 Encelle, Inc. Bioartificial devices and cellular matrices therefor
WO2000002999A2 (en) * 1998-07-10 2000-01-20 Encelle, Inc. Medium and matrix for long-term proliferation of cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834005A (en) * 1992-02-24 1998-11-10 Encelle, Inc. Bioartificial devices and cellular matrices therefor
WO2000002999A2 (en) * 1998-07-10 2000-01-20 Encelle, Inc. Medium and matrix for long-term proliferation of cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CORSO CARLOS O ET AL: "Effects of the nitric oxide synthase inhibitors NG-nitro-L-arginine methyl ester and aminoethyl-isothiourea on the liver microcirculation in rat endotoxemia.", JOURNAL OF HEPATOLOGY, vol. 28, no. 1, January 1998 (1998-01-01), pages 61 - 69, XP000990665, ISSN: 0168-8278 *
MUELLER ANDREA R ET AL: "The effects of administration of nitric oxide inhibitors during small bowel preservation and reperfusion.", TRANSPLANTATION (BALTIMORE), vol. 58, no. 12, 1994, pages 1309 - 1316, XP000990666, ISSN: 0041-1337 *

Also Published As

Publication number Publication date
AU7382300A (en) 2001-04-17
WO2001019962A2 (en) 2001-03-22

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