WO2001019853A3 - Cell transfection - Google Patents

Cell transfection Download PDF

Info

Publication number
WO2001019853A3
WO2001019853A3 PCT/GB2000/003462 GB0003462W WO0119853A3 WO 2001019853 A3 WO2001019853 A3 WO 2001019853A3 GB 0003462 W GB0003462 W GB 0003462W WO 0119853 A3 WO0119853 A3 WO 0119853A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
tissue
cell transfection
transfected
transfected cell
Prior art date
Application number
PCT/GB2000/003462
Other languages
French (fr)
Other versions
WO2001019853A2 (en
Inventor
Anthony Peter Hollander
Michael David Barker
Waeh Kafienah
Original Assignee
Univ Sheffield
Anthony Peter Hollander
Michael David Barker
Waeh Kafienah
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Sheffield, Anthony Peter Hollander, Michael David Barker, Waeh Kafienah filed Critical Univ Sheffield
Priority to AU73003/00A priority Critical patent/AU7300300A/en
Publication of WO2001019853A2 publication Critical patent/WO2001019853A2/en
Publication of WO2001019853A3 publication Critical patent/WO2001019853A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

An isolated nucleic acid molecule comprising at least the functional part of the blasticidin resistance (BS) gene, or homologue thereof, linked via a recognition sequence, to at least one selected gene; a method for the preparation thereof, a method for transfecting cells, a transfected cell and tissue comprising a transfected cell, a method for 'knockout', a method for incubating mitotically inactive cells, a therapeutic composition comprising transfected cells or tissue, and the use thereof.
PCT/GB2000/003462 1999-09-11 2000-09-11 Cell transfection WO2001019853A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU73003/00A AU7300300A (en) 1999-09-11 2000-09-11 Cell transfection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9921418.1A GB9921418D0 (en) 1999-09-11 1999-09-11 Cell transfection
GB9921418.1 1999-09-11

Publications (2)

Publication Number Publication Date
WO2001019853A2 WO2001019853A2 (en) 2001-03-22
WO2001019853A3 true WO2001019853A3 (en) 2001-11-15

Family

ID=10860700

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/003462 WO2001019853A2 (en) 1999-09-11 2000-09-11 Cell transfection

Country Status (3)

Country Link
AU (1) AU7300300A (en)
GB (1) GB9921418D0 (en)
WO (1) WO2001019853A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7585667B2 (en) 2003-06-13 2009-09-08 The United States Of America As Represented By The Department Of Health And Human Services Negative strand RNA virus replicon

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0648838A1 (en) * 1993-10-06 1995-04-19 Amgen Inc. Tissue inhibitor of metalloproteinase type three (TIMP-3)
US5527701A (en) * 1992-08-25 1996-06-18 The Institute Of Physical And Chemical Research Aspergillus terreus blasticidin S deaminase, a blasticidin S deaminase gene, a vector incorporating said gene and a transformant containing said vector
US5693508A (en) * 1994-11-08 1997-12-02 Chang; Lung-Ji Retroviral expression vectors containing MoMLV/CMV-IE/HIV-TAR chimeric long terminal repeats
WO1999028452A1 (en) * 1997-11-28 1999-06-10 Mochida Pharmaceutical Co., Ltd. Matrilysin antisense compounds
WO1999043795A1 (en) * 1998-02-24 1999-09-02 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Trans-somatics with gene transfer into mammary epithelial cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5527701A (en) * 1992-08-25 1996-06-18 The Institute Of Physical And Chemical Research Aspergillus terreus blasticidin S deaminase, a blasticidin S deaminase gene, a vector incorporating said gene and a transformant containing said vector
EP0648838A1 (en) * 1993-10-06 1995-04-19 Amgen Inc. Tissue inhibitor of metalloproteinase type three (TIMP-3)
US5693508A (en) * 1994-11-08 1997-12-02 Chang; Lung-Ji Retroviral expression vectors containing MoMLV/CMV-IE/HIV-TAR chimeric long terminal repeats
WO1999028452A1 (en) * 1997-11-28 1999-06-10 Mochida Pharmaceutical Co., Ltd. Matrilysin antisense compounds
WO1999043795A1 (en) * 1998-02-24 1999-09-02 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Trans-somatics with gene transfer into mammary epithelial cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 1 January 1900 (1900-01-01), "PLASMID PAMS WITH HYBRID AMPHOTROPIC/MOLONEY MURINE LEUKEMIA VIRUS, COMPLETE SEQUENCE", XP002168756, Database accession no. AF010170 *
DATABASE EMBL 1 January 1900 (1900-01-01), LOU Y, HOLTZ A: "CLONING VECTOR PIRES1HYG, COMPLETE PLASMID SEQUENCE", XP002168755, Database accession no. U89672 *
DATABASE WPI Section Ch Week 199930, Derwent World Patents Index; Class B04, AN 1999-358127, XP002168757 *
MASAKO ISUMI ET AL: "BLASTICIDIN S-RESISTANCE GENE (BSR): A NOVEL SELECTABLE MARKER FOR MAMMALIAN CELLS", EXPERIMENTAL CELL RESEARCH,US,SAN DIEGO, CA, vol. 197, no. 2, 1 December 1991 (1991-12-01), pages 229 - 233, XP000196772, ISSN: 0014-4827 *
URABE M ET AL: "A novel dicistronic AAV vector using a short IRES segment derived from hepatitis C virus genome", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES,GB,ELSEVIER SCIENCE PUBLISHERS, BARKING, vol. 200, no. 1-2, 24 October 1997 (1997-10-24), pages 157 - 162, XP004126490, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
GB9921418D0 (en) 1999-11-10
WO2001019853A2 (en) 2001-03-22
AU7300300A (en) 2001-04-17

Similar Documents

Publication Publication Date Title
DK1105507T3 (en) Overexpression of desired proteins in eukaryotic cells mediated by cyclin D1 overexpression
EP2278022A3 (en) Expression vectors, transfection systems, and method of use thereof
WO2002014525A3 (en) Expression vector using for animal cell
CA2335393A1 (en) Liposomal encapsulated nucleic acid-complexes
PL331005A1 (en) Regulating dna of hamster's ef-1alpha transcription
AU716500B2 (en) Nucleic acid constructs for the cell cycle-regulated expression of genes, cells containing such constructs and their use for preparing drugs
CA2159626A1 (en) Method for delivering beneficial compositions to hair follicles
WO1999064576A3 (en) Human genes differentially expressed in colon cancer
DE59913833D1 (en) STRUCTURAL PROTEIN OF AAV, ITS MANUFACTURE AND USE
EP1690939B8 (en) Novel G protein-coupled receptor proteins
EE03955B1 (en) Use of nucleic acid for the manufacture of a medicament for transfecting at least some cells of the mammalian reproductive tract
WO1999053046A3 (en) Noncloning technique for expressing a gene of interest
CA2280290A1 (en) Netrin receptors
IL133494A0 (en) Isolated dendritic cell membrane protein genes
WO1999054435A3 (en) Immortalised cell lines derived from normal human skin tissues
WO2000030667A3 (en) Compositions and methods using lactadherin or variants thereof
AU3107699A (en) Novel forms of t cell costimulatory proteins, nucleic acid molecules, and uses thereof
BR9811014A (en) "concentrated gene regulatory region specifically for early seed"
EP1020529A3 (en) Gene delivery vectors provided with a tissue tropism for smooth muscle cells, and/or endothelial cells
AU5331796A (en) Vectors for the transfection of eucaryotic cells, their use and target cells transfected thereby
WO2001019853A3 (en) Cell transfection
WO1996009399A3 (en) Chimeric adenovirus for gene delivery
AU5026600A (en) Methods of regulating the condition of mammalian keratinous tissue
AU4255899A (en) Method for non-radioactive detection of membrane-bonded nucleic acids and test kit
WO1999022005A3 (en) Heparan sulfate d-glucosaminyl 3-o-sulfotransferases, an uses therefor

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP