WO2001016371A3 - Procede d'identification de phosphoramidites a une base d'acide nucleique par la spectroscopie a fluorescence - Google Patents

Procede d'identification de phosphoramidites a une base d'acide nucleique par la spectroscopie a fluorescence Download PDF

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Publication number
WO2001016371A3
WO2001016371A3 PCT/US2000/023422 US0023422W WO0116371A3 WO 2001016371 A3 WO2001016371 A3 WO 2001016371A3 US 0023422 W US0023422 W US 0023422W WO 0116371 A3 WO0116371 A3 WO 0116371A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid base
phosphoramidites
fluorescence spectroscopy
identifying nucleic
Prior art date
Application number
PCT/US2000/023422
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English (en)
Other versions
WO2001016371A2 (fr
Inventor
Matthew C Friedenberg
Michael M Becker
Mehrdad Majlessi
James Russell
William G Weisburg
Original Assignee
Gen Probe Inc
Matthew C Friedenberg
Michael M Becker
Mehrdad Majlessi
James Russell
William G Weisburg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc, Matthew C Friedenberg, Michael M Becker, Mehrdad Majlessi, James Russell, William G Weisburg filed Critical Gen Probe Inc
Priority to AU68011/00A priority Critical patent/AU6801100A/en
Publication of WO2001016371A2 publication Critical patent/WO2001016371A2/fr
Publication of WO2001016371A3 publication Critical patent/WO2001016371A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédés de détection et d'identification de phosphoramidites à une base d'acide nucléique individuelles dans une solution échantillon par appariement de spectres d'excitation et/ou d'émission de fluorescence avec des spectres similaires de phosphoramidites connues. Des procédés permettant de déterminer la précision de la synthèse d'acide nucléique par analyse des spectres de fluorescence d'échantillons (influent, effluent, mélanges de réaction) associés à des étapes de liaison individuelles qui utilisent les phosphoramidites à une base d'acide nucléique, et d'identifier la phosphoramidite contenue dans chaque échantillon sont également décrits.
PCT/US2000/023422 1999-08-27 2000-08-25 Procede d'identification de phosphoramidites a une base d'acide nucleique par la spectroscopie a fluorescence WO2001016371A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU68011/00A AU6801100A (en) 1999-08-27 2000-08-25 Method for identifying nucleic acid base phosphoramidites by fluorescence spectroscopy

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15111699P 1999-08-27 1999-08-27
US60/151,116 1999-08-27

Publications (2)

Publication Number Publication Date
WO2001016371A2 WO2001016371A2 (fr) 2001-03-08
WO2001016371A3 true WO2001016371A3 (fr) 2002-07-11

Family

ID=22537386

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/023422 WO2001016371A2 (fr) 1999-08-27 2000-08-25 Procede d'identification de phosphoramidites a une base d'acide nucleique par la spectroscopie a fluorescence

Country Status (2)

Country Link
AU (1) AU6801100A (fr)
WO (1) WO2001016371A2 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001214A1 (fr) * 1992-07-06 1994-01-20 Beckman Instruments, Inc. Procede de surveillance de la reaction et de l'ecoulement d'un processus en ligne
WO1995026416A1 (fr) * 1994-03-25 1995-10-05 Research Corporation Technologies, Inc. Procede de diagnostic par biocapteurs d'acide nucleique
WO1997036960A1 (fr) * 1996-04-01 1997-10-09 The Perkin-Elmer Corporation Colorants asymetriques a base de benzoxanthene
WO1999013110A1 (fr) * 1997-09-11 1999-03-18 Seq, Ltd. Procede de fabrication de photoproduits de nucleotides fluorescents pour sequençage d'adn et analyse
EP0984021A2 (fr) * 1998-08-03 2000-03-08 Hewlett-Packard Company Synthèse d'oligonucléotides

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001214A1 (fr) * 1992-07-06 1994-01-20 Beckman Instruments, Inc. Procede de surveillance de la reaction et de l'ecoulement d'un processus en ligne
WO1995026416A1 (fr) * 1994-03-25 1995-10-05 Research Corporation Technologies, Inc. Procede de diagnostic par biocapteurs d'acide nucleique
WO1997036960A1 (fr) * 1996-04-01 1997-10-09 The Perkin-Elmer Corporation Colorants asymetriques a base de benzoxanthene
WO1999013110A1 (fr) * 1997-09-11 1999-03-18 Seq, Ltd. Procede de fabrication de photoproduits de nucleotides fluorescents pour sequençage d'adn et analyse
EP0984021A2 (fr) * 1998-08-03 2000-03-08 Hewlett-Packard Company Synthèse d'oligonucléotides

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DNA SEQUENCING USING A FOUR-COLOR CONFOCAL FLUORESCENCE CAPILLARY ARRAY SCANNER: "DNA SEQUENCING USING A FOUR-COLOR CONFOCAL FLUORESCENCE CAPILLARY ARRAY SCANNER", ELECTROPHORESIS, WEINHEIM, DE, vol. 17, no. 8, August 1996 (1996-08-01), pages 1852 - 1859, XP000901208, ISSN: 0173-0835 *
HAWKINS MARY E ET AL: "Fluorescence properties of pteridine nucleoside analogs as monomers and incorporated into oligonucleotides.", ANALYTICAL BIOCHEMISTRY, vol. 244, no. 1, 1997, pages 86 - 95, XP002185365, ISSN: 0003-2697 *
SRIVASTAVA SURESH C ET AL: "1,N-6-etheno deoxy and ribo adenosine and 3,N-4-ethano deoxy and ribo cytidine phosphoramidites. Strongly fluorescent structures for selective introduction in defined sequence DNA and RNA molecules.", NUCLEIC ACIDS RESEARCH, vol. 22, no. 7, 1994, pages 1296 - 1304, XP002185366, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2001016371A2 (fr) 2001-03-08
AU6801100A (en) 2001-03-26

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