WO2001012839A1

WO2001012839A1 - Method for detecting, treating, and monitoring conditions associated with activation of the coagulation response

Info

Publication number: WO2001012839A1
Application number: PCT/US2000/022177
Authority: WO
Inventors: David E. Berg; Lois Hill Berg; Harold H. Harrison
Original assignee: Berg David E; Lois Hill Berg; Harrison Harold H
Priority date: 1999-08-13
Filing date: 2000-08-11
Publication date: 2001-02-22
Also published as: CA2385502C; CA2385502A1; AU783760B2; GB2368642A; GB0203838D0; GB2368642B; AU6769000A

Abstract

Methods for diagnosing, treating and monitoring conditions associated with activation of the coagulation response in patients suffering from conditions such as chronic fatigue syndrome, fibromyalgia and cardiovascular disease are disclosed. Diagnostic assays utilized in the methods include measurement of blood levels of fibrinogen, prothrombin fragment 1 + 2, thrombin/antithrombin complexes and soluble fibrin monomers. Treatment regimens include anticoagulant therapies comprising administering warfarin or heparin as needed.

Description

METHOD FOR DETECTING, TREATING, AND MONITORING CONDITIONS ASSOCIATED WITH ACTIVATION OF THE COAGULATION RESPONSE

FIELD OF THE INVENTION

This Non-Provisional Application claims the benefit of U.S. Provisional Application

Serial No. 60/148,799 filed on August 13, 1999. This invention relates to methods for detecting, treating, and monitoring conditions associated with activation of the coagulation response which respond to anti-coagulant therapy. BACKGROUND OF THE INVENTION

Chronic fatigue syndrome (CFS) and fibromyalgia (FM) have been considered diagnoses of exclusion where no other diagnosis fit well. CFS has been defined by specific requirements of fatigue, its duration, associated symptoms, and initial clinical and laboratory evaluation. There has existed no reliable laboratory means for determining whether an individual was suffering from CFS, FM, or some other disease. Accordingly, a felt need for a method of testing for CFS and FM existed. SUMMARY OF THE INVENTION

This invention relates to a method for using a novel combination of assays to detect minimal activation of the coagulation response for determining whether a patient whose initial clinical evaluation indicates chronic fatigue syndrome, fibromyalgia, and related conditions can be treated using anticoagulant therapies. If activation of the coagulation response is detected, the present invention further includes treatment of CFS, FM or related condition using anticoagulant therapies.

We have discovered that we may reliably diagnose a patient suffering from CFS or FM by evaluating the status of the coagulation response in that patient by using a novel combination of tests which can detect minimal activation of the coagulation response in a patient. This novel combination includes tests for determining levels of fibrinogen, prothrombin fragment 1 + 2, thrombin/antithrombin complexes, soluble fibrin monomer, and platelet activation by flow cytometry. These assays are highly sensitive to minimal deviation from normal. Deviation from the normal values in any two of the five assays permits diagnosis of CFS, FM, or other disease associated with activation of the coagulation response.

Once a condition associated with activation of the coagulation response has been diagnosed, the patient is treated with anticoagulant therapy, such as heparin followed by warfarin or warfarin alone. Coumarins or coumarin derivatives may also be used. Heparin can be defined as heparin (porcine or bovine) or any of its derivatives, such as low molecular weight heparin (LMWH), oral heparin, heparinoids, or any other designer heparin-like drugs. It is presently preferred that a low dose anticoagulant therapy be used. Patient progression and recovery is then monitored using the novel combination of assays. We postulate that a majority of individuals diagnosed as CFS and/or FM on clinical criteria may be defined as having antiphosphohpid antibody syndrome (APS) with endothelial cells as a major disease target with or without platelet activation. Laboratory findings in patients suffering with APS include anticardiolipin antibodies, lupus anticoagulants, antiphosphatidylserine antibodies, anti-B2GPI antibodies. Clinical findings include thrombocytopenia, neurological complications, venous thrombosis, arterial thrombosis, and/or recurrent fetal loss. Patients with primary APS (PAPS) presently have no clinical or laboratory evidence of another definable autoimmune disease. Antiphosphohpid antibodies have long been associated with a hypercoagulable state, involving both procoagulant activity as well as inhibition of anticoagulant and fibrinolytic activity. In CFS and/or FM patients, the principal antibodies found to date are the anti-B2GPI antibodies. This precedes the generation of a hypercoagulable state based on our proposed model. Endothelial cells are protected in the microvascular circulation by B₂GPI and Annexin

V proteins. This protective layer helps endothelial cells maintain an anticoagulant environment. Exposure to pathogens, such as herpes viruses (HV, HHV6, EBV), cytomegalovirus (CMV), mycoplasma, chlamydia pneumonia, or some vaccines can result in both active persistent infection and latent infection in mononuclear and endothelial cells. Some pathogens like CMV and HV constitutively express phosphatidylserine-like procoagulant activity, capable of binding Xa and Va to form the prothrombinase complex. HHV6 is found in about 70% of all CFS patients. In several studies, this same 70% infection rate is seen in multiple sclerosis patients with HHV6. HHV6 is also implicated in chronic myelopathy. Endothelial cells serve as a reservoir for harboring HHV6. Infected endothelial cells lose the ability to synthesize prostacyclin with associated incapacity to deter platelet adhesion. In addition, CMV and HV express tissue factor antigen on each virus surface. HV can induce a prothrombotic phenotype in vascular endothelial cells. This phenotype markedly reduces heparin sulfate protcoglycan synthesis and surface expression by endothelial cells. Thrombomodulin expression is also reduced in infected endothelium. Due to fibrin deposition, fibrinolysis activation may also be diminished. Activation of endothelial cells is seen by surface expression of P-selectin and von Willebrand Factor (vWF). Thrombin generated after the assembly of the prothrombinase complex on the virus-infected

SUBSTΓTUTE SHEET RULE 26 endothelium mobilizes vWF from the Weibel-Palade body to the endothelial cell surface, where it acts as a platelet receptor. Cell-independent thrombin generation may be the earliest event in vascular pathology mediated by HN.

Because exposure and expression of phosphatidylserine (PS) is part of the infectious process, those exposed phospholipids activate the immune system to form antiphosphohpid antibodies. The primary targets of these immunoglobulin (Ig)G, IgM and IgA antibodies are the protective proteins for endothelial cells, specifically B2GPI and Annexin V. As with other APS diseases, there is an increased incidence of thrombocytopenia in HHV6 patients. With the loss of this protective layer due to APL antibodies, coagulation proteins can bind, react and form thrombin (Ila). If this process is not property inhibited (thrombin-anti- thrombin complexes), then excess thrombin can convert fibrinogen to soluble fibrin monomer (SFM). SFM is a sticky protein that increases blood viscosity and can coat endothelial cells surfaces as fibrin or fibrinoid material. Patients with CFS and FM symptoms typically have a hypercoagulable state demonstrated by increased markers of coagulation activation and increased blood viscosity due to the generation of soluble fibrin monomer (SFM). Once CFS and FM is diagnosed using our combination of assays, CFS/FM patients may be treated with anticoagulant therapies, and their treatment and recovery monitored using our combination of tests.

Patients with immune mediated chronic inflammatory disorders of many types can have low level activation of the coagulation response. Therefore, patients with a spectrum of chronic inflammatory processes may have low level activation of coagulation as part of their pathophysiology. We postulate that our tests for activation of the coagulation and platelet systems also have application to other conditions which stem from activation of the coagulation response. This has been validated by preliminary studies of patients suffering with multiple sclerosis, breast implant sickness syndrome, fetal wastage syndrome, gulf war illness, inflammatory bowel disease, autism. As with CFS and FM, once diagnosed using our combination of assays, these patients may be treated with anticoagulant therapies, and their treatment and recovery monitored using our combination of tests.

We postulate that our combination of tests for detecting minimal activation of coagulation response also has application to detecting and treating, Sjogrens syndrome, late

Lyme disease (also called chronic Lyme disease), transient ischemic attack, attention deficit disorder, Alzheimer's disease, Parkinson's disease, as well as some cardiovascular diseases.

Once diagnosed using our combination of assays, these patients should also benefit from treatment with anticoagulant therapies, and their treatment and recovery monitored using our combination of tests.

The Presently Preferred Assays Used To Determine Minimal Activation of Coagulation Response

Although thrombin generation is among the first steps in the coagulation response, thrombin itself is difficult to quantify because it lasts only 30 seconds in the blood circulation.

Consequently, other measurable components of the coagulation response must be considered. (1) Prothrombin fragment 1+2, which is released when prothrombin is converted into thrombin, can be used to indicate activation of the coagulation response. (2) Increased levels of thrombin/antithrombin complexes (TATs) also indicate thrombin generation and an attempt to remove excess thrombin. Thus, an increased level of TATs also indicates activation of the coagulation response. (3) When excess thrombin is generated by the coagulation response, fibrinogen is cleaved to soluble fibrin monomer (SFM). Accordingly, increased levels of SFM also indicate activation of the coagulation response. (4) With the consumption of fibrinogen, the body may compensate by increasing fibrinogen levels slightly above the normal range; therefore, increased fibrinogen levels are also an indicator of activation of the coagulation response. (5) Finally, platelet activation may also be used as an indicator of coagulation response activation.

The tests discussed above are the assays presently preferred, it being understood that other tests sensitive to minimal activation of the coagulation response may be substituted for the assays discussed. DETAILED DESCRIPTION OF THE INVENTION

For the purposes of describing the invention, we discuss the form of the method of the present invention which is presently preferred; it being understood, however, that this invention is not limited to the precise arrangements, instrumentalities, and assays discussed. Our new method for diagnosing CFS, FM, and related conditions uses a novel combination of assays for detecting minimal activation of the coagulation response. Highly sensitive tests are used which are capable of detecting minimal deviations from normal levels of fibrinogen, prothrombin fragment 1 + 2, thrombin/antithrombin complexes, soluble fibrin monomer, and platelet activation by flow cytometry, platelet aggregation, or related techniques. According to our invention, if a patient receives an initial clinical evaluation indicating CFS and/or FM and if 2 or more of the above assays show abnormal results, that patient can be reliably diagnosed with CFS and/or FM. Once a condition has been diagnosed that is responsive to anti-coagulant therapy, the patient is treated with low dose anticoagulant therapy, such as heparin followed by warfarin, or pharmacologically and conceptually similar anticoagulant regimens. Patient progression and recovery is then monitored using the novel combination of assays.

In order to test our method, we have conducted a blinded study of 54 CFS and/or FM patients and 23 controls, for a total of 77 individuals. According to our method, blood drawn from all individuals was subjected to each of the 5 tests. Using our presently preferred combination of 5 assays, we determined that the CFS and FM patients could be reliably distinguished from the controls. Individuals whose blood tested outside of the normal range for 2 or more of the 5 tests were diagnosed as suffering from CFS and/or FM. 22 of the 23 controls were correctly identified. One control was positive in two assays for a false positivity rate of 4%. Of the 54 patients with CFS or FM, 4 had normal values, for a false negative rate of 7.4%. This shows that 92+% of CFS and/or FM patients had a demonstrable hypercoagulable state.

The following table sets out the mean values for each group tested.

As stated above, in CFS and FM patients, the principal antibodies found to date are the anti-B2GPI antibodies. Anti-B2GPI antibodies can precede the generation of a hypercoagulable state. In a normal capillary endothelial cell, phosphatidylserine is on the inside of the cell membrane. When phosphatidylserine is transported to the surface of the endothelial cell, it is isolated from blood flowing through the vessels by a layer of B2GPI and Annexin V proteins, which cover the phosphatidylserine. When the immune system is activated, anti-B2GPI and anti-annexin V antibodies, collectively antiphosphohpid antibodies, are generated. These antibodies are IgG, IgA, and IgM, the majority of which bind to the B2GPI and annexin V proteins dislodging them from the phosphatidylserine. When the phosphatidylserine on the vessel walls is exposed, coagulation factor tenase and prothrombinase complexes bind to the surfaces of the endothelial cells which coat the vessel walls. When prothrombin complexes bind to the cell walls, prothrombin is converted to thrombin, which releases the activation peptide prothrombin fragment 1 + 2.

The human body responds to the generation of excess or inappropriate thrombin levels by synthesizing the inhibitor antithrombin. Antithrombin combines with thrombin to form thrombin/antithrombin complexes, which are then removed from the blood. Antithrombin is a slow inhibitor of thrombin, but in the presence of heparin, antithrombin reacts with thrombin at a greatly increased rate. Activated protein C/antitrypsin complex is a secondary inhibitor of thrombin generation.

When more thrombin is generated than can be removed by the thrombin inhibitors, thrombin reacts with fibrinogen to create an intermediate protein called soluble fibrin monomer. Soluble fibrin monomer is a sticky protein which increases blood viscosity and forms deposits on capillary wall endothelial cells. The soluble fibrin monomer which is deposited on the capillary walls, a phenomenon called fibrin deposition, may block the passage of nutrients through the capillary walls to the surrounding tissues, whether it is another capillary, muscle or organ tissue. We postulate that the increase in blood viscosity in combination with associated blockage in nutrient transfer results in the fatigue of CFS and the muscle pain of FM. This could also be part of the explanation (pathology) of fetal demise and spontaneous abortions seen in recurrent miscarriages (fetal wastage syndrome).

In addition to the creation of soluble fibrin monomer, thrombin also activates factor Xm to XlUa in the blood, causing it to react with the soluble fibrin monomer to create insoluble fibrin strands, which may in turn generate a clot or fibrin deposition on vascular walls, further inhibiting blood flow and nutrient transfer. Once fibrin deposition or blood clot formation occurs, it becomes necessary to dissolve the fibrin. Protein C, which is activated by the excess thrombin production, helps to activate the fibrinolytic system when tissue plasminogen activator converts the protein plasminogen into plasmin. Plasmin is a very potent enzyme for fibrin depositions or clots and keeps the circulatory system of blood vessels open and clean. Tissue plasminogen activator is conventionally injected into patients to dissolve blood clots, such as heart attacks, myocardial infarctions, strokes, blood clots in the eye, etc.

Platelet activation within the blood is also a contributing factor in thrombin formation. Platelet activation reinforces the generation of thrombin by providing a major surface for thrombin formation. Platelets may be directly activated by the immune system when immunoglobulins activate glycoprotein receptors and cause platelets to become "sticky". This "sticky state" can result in fibrin formation, vascular and endothelial cell changes, and, at times, platelet clumping. Platelet activation can be measured by a special laboratory procedure called flow cytometry, platelet aggregation or related techniques. Differences in genetic makeup are the most likely reason that all people exposed to an immune system activating pathogen do not develop diseases associated with a hypercoagulable state, such as chronic fatigue syndrome and fibromyalgia. Patients who have a hereditary deficiency for thrombophilia or hypofibrinolysis may be unable to properly control thrombin generation. Genetic defects in the proteins which regulate the coagulation process, are most likely the cause of long term activation of the immune system which results in a persistent hypercoagulable state. Thrombophilia may be caused by one or more genetic abnormalities in proteins which presently include protein C, protein S, antithrombin, factor V leiden, factor II gene mutation, thrombomodulin, heparin cofactor II, or factors II, VII, IX, X, XI, XII, and others which may be found for related genes. Hypofibrinolysis may be caused by one or more genetic abnormalities factors which presently include plasminogen, tPA, urokinase, PAI-1, lipoprotein(a), homocysteine, factor V leiden, factor XI, and TAFI, and others which may be found for related genes. Patients with immune mediated chronic inflammatory disorders of many types can have low level activation of the coagulation response. Therefore, patients with a spectrum of chronic inflammatory processes may have low level activation of coagulation as part of their pathophysiology. We postulate that our tests for activation of the coagulation and platelet systems also have application to other conditions which stem from activation of the coagulation response. This has been validated by preliminary studies of patients suffering with multiple sclerosis, breast implant sickness syndrome, gulf war illness, inflammatory bowel disease, autism, and fetal wastage syndrome. As with CFS and FM, once diagnosed using our combination of assays, these patients may be treated with anticoagulant therapies, and their treatment and recovery monitored using our combination of tests. We propose that a common feature of these conditions is the chronic inflammatory process that stimulates low level activation of coagulation.

The following table sets out our preliminary findings for autism, attention deficit disorder, multiple sclerosis, Parkinson's disease, Sjogrens syndrome, and gulf war illness. Individuals who were diagnosed as suffering from one of these chronic inflammatory disorders tested outside of the normal range for 2 or more of the 5 tests in our method.

We postulate that our combination of tests for detecting minimal activation of coagulation response also has application to detecting and treating other immune mediated chronic inflammatory disorders such as Sjogrens syndrome, late Lyme disease (also called chronic Lyme disease), transient ischemic attack, attention deficit disorder, Alzheimer's disease, Parkinson's disease, as well as some cardiovascular diseases. Once diagnosed using our combination of assays, these patients should also benefit from treatment with anticoagulant therapies, and their treatment and recovery monitored using our combination of tests.

Claims

WE CLAIM:

1. A method for diagnosing a condition associated with activation of the coagulation response comprising: a. Testing the blood of a patient to determine if activation of the coagulation response is indicated.

2. A method for diagnosing and treating a condition associated with activation of the coagulation response comprising: a. Testing the blood of a patient to determine if activation of the coagulation response is present; b. Determining that there is activation of the coagulation response; and c. Treating the patient using low dose anticoagulant therapy.

3. A method for diagnosing, treating and monitoring a condition associated with activation of the coagulation response comprising: a. Utilizing a grouping of blood tests to determine if activation of the coagulation response is present; b. Determining that there is activation of the coagulation response; c. Treating the patient using low dose anticoagulant therapy; and d. Monitoring the recovery using said grouping of blood tests.

4. The method of claim 3 wherein said grouping of blood tests comprises: a. A test to determine the level of fibrinogen; b. A test to determine the level of prothrombin fragment 1+2; c. A test to determine the level of thrombin/antithrombin complexes; and d. A test to determine the level of soluable fibrin monomer.

5. The method of claim 4 wherein said condition is diagnosed by at least two of said tests in said grouping of blood tests indicating activation of the coagulation response.

6. The method of claim 5 wherein said condition is chronic fatigue syndrome.

7. The method of claim 5 wherein said condition is fibromyalgia.

8. The method of claim 5 wherein said condition is multiple sclerosis.

9. The method of claim 5 wherein said condition is gulf war illness.

10. The method of claim 5 wherein said condition is breast implant sickness syndrome.

11. The method of claim 5 wherein said condition is inflammatory bowel disease.

12. The method of claim 5 wherein said condition is autism.

13. The method of claim 5 wherein said condition is Sjorens syndrome.

14. The method of claim 5 wherein said condition is late Lyme disease.

15. The method of claim 5 wherein said condition transient ischemic attack.

16. The method of claim 5 wherein said condition is attention deficit disorder.

17. The method of claim 5 wherein said condition is Alzheimer's disease.

18. The method of claim 5 wherein said condition is Parkinson's disease.

19. The method of claim 5 wherein said condition is fetal wastage syndrome.

20. The method of claim 5 wherein said condition is a cardiovascular disease.

21. The method of claim 3 wherein said grouping of blood tests comprises: a. A test to determine the level of fibrinogen; b. A test to determine the level of prothrombin fragment 1+2; c. A test to determine the level of thrombin/antithrombin complexes; and d. A test to determine the level of soluble fibrin monomer; and e. A test to determine the level of platelet activation.

22. The method of claim 21 wherein said condition is diagnosed by at least two of said tests in said grouping of blood tests indicating activation of the coagulation response.

23. The method of claim 22 wherein said condition is chronic fatigue syndrome.

24. The method of claim 22 wherein said condition is fibromyalgia.

25. The method of claim 22 wherein said condition is multiple sclerosis.

26. The method of claim 22 wherein said condition is gulf war illness.

27. The method of claim 22 wherein said condition is breast implant sickness syndrome.

28. The method of claim 22 wherein said condition is inflammatory bowel disease.

29. The method of claim 22 wherein said condition is autism.

30. The method of claim 22 wherein said condition is Sjorens syndrome.

31. The method of claim 22 wherein said condition is late Lyme disease.

32. The method of claim 22 wherein said condition transient ischemic attack.

33. The method of claim 22 wherein said condition is attention deficit disorder.

34. The method of claim 22 wherein said condition is Alzheimer's disease.

35. The method of claim 22 wherein said condition is Parkinson's disease.

36. The method of claim 22 wherein said condition is fetal wastage syndrome.

37. The method of claim 22 wherein said condition is a cardiovascular disease.

38. The method of claim 5 wherein said low dose anticoagulant therapy is heparin.

39. The method of claim 5 wherein said low dose anticoagulant therapy is heparin followed by warfarin.

40. The method of claim 5 wherein said low dose anticoagulant therapy is warfarin.

41. The method of claim 22 wherein said low dose anticoagulant therapy is heparin.

42. The method of claim 22 wherein said low dose anticoagulant therapy is heparin followed by warfarin.

43. The method of claim 22 wherein said low dose anticoagulant therapy is warfarin.