WO2001011945A3 - Methods for mass production of plantlets - Google Patents

Methods for mass production of plantlets Download PDF

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Publication number
WO2001011945A3
WO2001011945A3 PCT/US2000/022127 US0022127W WO0111945A3 WO 2001011945 A3 WO2001011945 A3 WO 2001011945A3 US 0022127 W US0022127 W US 0022127W WO 0111945 A3 WO0111945 A3 WO 0111945A3
Authority
WO
WIPO (PCT)
Prior art keywords
plantlets
nodal
fragments
blade
disease
Prior art date
Application number
PCT/US2000/022127
Other languages
French (fr)
Other versions
WO2001011945A2 (en
Inventor
Andrew Watson
Original Assignee
Andrew Watson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Andrew Watson filed Critical Andrew Watson
Publication of WO2001011945A2 publication Critical patent/WO2001011945A2/en
Publication of WO2001011945A3 publication Critical patent/WO2001011945A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/003—Cutting apparatus specially adapted for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002—Culture media for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques

Abstract

A method for automated mass production of tissue culture plantlets (P1A, P1B), wherein disease-free plantlets (P1A, P1B) grown in vitro are mechanically sectioned into nodal fragments (NF1'-5') by means of a tissue sectioning apparatus (40, 42, 44a-d) having at least one blade (S1-S3). The at least one blade (S1-S3) is adapted for performing repetitive motion and for repeatedly sectioning plant tissue. A plurality of plantlets (P1A, P1B) may be contacted with the at least one blade (S1-S3) while the plurality of plantlets (P1A, P1B) are attached to a culture medium (16) in a culture container (10). Preferably, the sectioning apparatus (40, 42, 44a-d) includes a plurality of blades (S1-S3) substantially parallel to each other, wherein the spacing between the plurality of blades (S1-S3) is adjustable. Nodal fragments (NF1'-5') may in turn be cultured in vitro to provide a further plurality of plantlets (P1A, P1B). After an appropriate number of disease-free plantlets (P1A, P1B) have been obtained, nodal fragments (NF1'-5') excised therefrom may be grown in a suitable medium (16) under non-aseptic conditions to provide a plurality of plantlets (P1A, P1B) which are substantially disease-free and suitable for transplanting to the field for the production of potato minitubers.
PCT/US2000/022127 1999-08-17 2000-08-11 Methods for mass production of plantlets WO2001011945A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37591099A 1999-08-17 1999-08-17
US09/375,910 1999-08-17

Publications (2)

Publication Number Publication Date
WO2001011945A2 WO2001011945A2 (en) 2001-02-22
WO2001011945A3 true WO2001011945A3 (en) 2001-09-27

Family

ID=23482868

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/022127 WO2001011945A2 (en) 1999-08-17 2000-08-11 Methods for mass production of plantlets

Country Status (1)

Country Link
WO (1) WO2001011945A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101336612B (en) * 2007-07-04 2012-05-23 青海省农林科学院 Potato test-tube plantlet assembly type fragment propagating method
CN113970472B (en) * 2020-07-23 2023-08-22 北京仁诚神经肿瘤生物技术工程研究中心有限公司 Tissue slice method and molding device for tissue slice

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4569914A (en) * 1982-06-28 1986-02-11 Gyogynoveny Kutato Intezet Process for the sterile micropropagation of plant material
US4774186A (en) * 1985-11-05 1988-09-27 Schaefer Jr Jimmie W Microbial compositions and methods for treating soil
US5088231A (en) * 1987-03-04 1992-02-18 Agristar, Inc. Automated system for micropropagation and culturing organic material
US5141866A (en) * 1983-07-26 1992-08-25 Robert Levin Process for plant tissue culture propagation
US5225345A (en) * 1990-07-23 1993-07-06 Kirin Beer Kabushiki Kaisha Plant tissue cutting and transplanting apparatus
US5370713A (en) * 1990-09-07 1994-12-06 The Commonwealth Industrial Gases Limited Automatic plant dividing system
US5382268A (en) * 1989-09-21 1995-01-17 British Technology Group Ltd. Method and apparatus for use in micropropagation
US5419079A (en) * 1993-01-07 1995-05-30 Tianjin Research Institute Of Vegatable Method of producing virus free potato minitubers
US5498541A (en) * 1993-11-30 1996-03-12 Japan Tobacco Inc. Method for producing potato microtubers
US5792934A (en) * 1993-04-30 1998-08-11 The United States Of America As Represented By The Secretary Of Agriculture Enhanced insect resistance in plants genetically engineered with a plant hormone gene involved in cytokinin biosynthesis

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4569914A (en) * 1982-06-28 1986-02-11 Gyogynoveny Kutato Intezet Process for the sterile micropropagation of plant material
US5141866A (en) * 1983-07-26 1992-08-25 Robert Levin Process for plant tissue culture propagation
US4774186A (en) * 1985-11-05 1988-09-27 Schaefer Jr Jimmie W Microbial compositions and methods for treating soil
US5088231A (en) * 1987-03-04 1992-02-18 Agristar, Inc. Automated system for micropropagation and culturing organic material
US5382268A (en) * 1989-09-21 1995-01-17 British Technology Group Ltd. Method and apparatus for use in micropropagation
US5225345A (en) * 1990-07-23 1993-07-06 Kirin Beer Kabushiki Kaisha Plant tissue cutting and transplanting apparatus
US5370713A (en) * 1990-09-07 1994-12-06 The Commonwealth Industrial Gases Limited Automatic plant dividing system
US5419079A (en) * 1993-01-07 1995-05-30 Tianjin Research Institute Of Vegatable Method of producing virus free potato minitubers
US5792934A (en) * 1993-04-30 1998-08-11 The United States Of America As Represented By The Secretary Of Agriculture Enhanced insect resistance in plants genetically engineered with a plant hormone gene involved in cytokinin biosynthesis
US5498541A (en) * 1993-11-30 1996-03-12 Japan Tobacco Inc. Method for producing potato microtubers

Also Published As

Publication number Publication date
WO2001011945A2 (en) 2001-02-22

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