WO2001011341A3 - Systeme criblage de cellules - Google Patents

Systeme criblage de cellules Download PDF

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Publication number
WO2001011341A3
WO2001011341A3 PCT/US2000/021426 US0021426W WO0111341A3 WO 2001011341 A3 WO2001011341 A3 WO 2001011341A3 US 0021426 W US0021426 W US 0021426W WO 0111341 A3 WO0111341 A3 WO 0111341A3
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WO
WIPO (PCT)
Prior art keywords
cells
cell
based screening
kits
screens
Prior art date
Application number
PCT/US2000/021426
Other languages
English (en)
Other versions
WO2001011341A2 (fr
Inventor
Keith Olson
Oleg Lapets
Gary Bright
Randall O Shopoff
Original Assignee
Cellomics Inc
Keith Olson
Oleg Lapets
Gary Bright
Randall O Shopoff
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc, Keith Olson, Oleg Lapets, Gary Bright, Randall O Shopoff filed Critical Cellomics Inc
Priority to AU66228/00A priority Critical patent/AU6622800A/en
Publication of WO2001011341A2 publication Critical patent/WO2001011341A2/fr
Publication of WO2001011341A3 publication Critical patent/WO2001011341A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
    • G01N33/5079—Mitochondria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne des systèmes, des techniques, des cribles, des réactifs, et des kits destinés à l'analyse de cellules d'un système optique, afin de déterminer rapidement la répartition, l'environnement, ou l'activité de molécules reporteurs marquées par fluorescence dans des cellules, et de cribler un grand nombre de composés qui affectent de manière spécifique l'indice mitotique desdites cellules.
PCT/US2000/021426 1999-08-05 2000-08-04 Systeme criblage de cellules WO2001011341A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU66228/00A AU6622800A (en) 1999-08-05 2000-08-04 A system for cell-based screening

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US14725499P 1999-08-05 1999-08-05
US60/147,254 1999-08-05
US39896599A 1999-09-17 1999-09-17
US09/398,965 1999-09-17
US17435600P 2000-01-04 2000-01-04
US60/174,356 2000-01-04
US17671500P 2000-01-18 2000-01-18
US60/176,715 2000-01-18

Publications (2)

Publication Number Publication Date
WO2001011341A2 WO2001011341A2 (fr) 2001-02-15
WO2001011341A3 true WO2001011341A3 (fr) 2001-07-05

Family

ID=27495751

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/021426 WO2001011341A2 (fr) 1999-08-05 2000-08-04 Systeme criblage de cellules

Country Status (2)

Country Link
AU (1) AU6622800A (fr)
WO (1) WO2001011341A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8953866B2 (en) 2004-03-16 2015-02-10 Amnis Corporation Method for imaging and differential analysis of cells

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8406498B2 (en) 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US8131053B2 (en) 1999-01-25 2012-03-06 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US7450229B2 (en) 1999-01-25 2008-11-11 Amnis Corporation Methods for analyzing inter-cellular phenomena
US8885913B2 (en) 1999-01-25 2014-11-11 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US6986993B1 (en) 1999-08-05 2006-01-17 Cellomics, Inc. System for cell-based screening
US6716588B2 (en) 1999-12-09 2004-04-06 Cellomics, Inc. System for cell-based screening
AU2002230415A1 (en) 2000-11-17 2002-05-27 Universal Imaging Corporation Rapidly changing dichroic beamsplitter
DK1348124T3 (da) 2000-12-22 2004-08-09 Cellomics Inc Identifikation af individuelle celler under kinetiske assays
US7907765B2 (en) 2001-03-28 2011-03-15 University Of Washington Focal plane tracking for optical microtomography
US6636623B2 (en) * 2001-08-10 2003-10-21 Visiongate, Inc. Optical projection imaging system and method for automatically detecting cells with molecular marker compartmentalization associated with malignancy and disease
US7260253B2 (en) 2002-04-19 2007-08-21 Visiongate, Inc. Method for correction of relative object-detector motion between successive views
US7738945B2 (en) 2002-04-19 2010-06-15 University Of Washington Method and apparatus for pseudo-projection formation for optical tomography
GB0307684D0 (en) * 2003-04-02 2003-05-07 Amersham Biosciences Uk Ltd Determining cell cycle phase data
EP1616286A1 (fr) * 2003-04-02 2006-01-18 Amersham Biosciences UK Limited Procede et logiciel associe pour la classification de cellules en sous-populations
CA2598602A1 (fr) 2004-03-16 2005-10-20 Amnis Corporation Quantification par image de la translocation moleculaire
EP2027465A2 (fr) 2006-05-17 2009-02-25 Cellumen, Inc. Procédé d'analyse automatique des tissus
JP4855139B2 (ja) 2006-05-18 2012-01-18 オリンパス株式会社 顕微鏡装置および細胞観察方法
US7835561B2 (en) 2007-05-18 2010-11-16 Visiongate, Inc. Method for image processing and reconstruction of images for optical tomography
US7787112B2 (en) 2007-10-22 2010-08-31 Visiongate, Inc. Depth of field extension for optical tomography
EP2112509A1 (fr) * 2008-03-25 2009-10-28 4Sc Ag Analyse de centrosome
WO2012125807A2 (fr) 2011-03-17 2012-09-20 Cernostics, Inc. Systèmes et compositions pour le diagnostic de l'œsophage de barrett et leurs procédés d'utilisation
US11069054B2 (en) 2015-12-30 2021-07-20 Visiongate, Inc. System and method for automated detection and monitoring of dysplasia and administration of immunotherapy and chemotherapy

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (fr) * 1996-05-30 1997-12-04 Biodx Procedes et appareil a groupement miniaturise de cellules pour le criblage sur la base de cellules
WO1998038490A1 (fr) * 1997-02-27 1998-09-03 Cellomics, Inc. Systeme de criblage de cellules
WO2000003246A2 (fr) * 1998-07-13 2000-01-20 Cellomics, Inc. Systeme destine a un criblage a base de cellules
WO2000017643A2 (fr) * 1998-09-18 2000-03-30 Cellomics, Inc. Systeme de criblage cellulaire
WO2000050872A2 (fr) * 1999-02-26 2000-08-31 Cellomics, Inc. Systeme de criblage cellulaire
WO2000070342A2 (fr) * 1999-05-14 2000-11-23 Cellomics, Inc. Un systeme de criblage a base de cellules

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (fr) * 1996-05-30 1997-12-04 Biodx Procedes et appareil a groupement miniaturise de cellules pour le criblage sur la base de cellules
WO1998038490A1 (fr) * 1997-02-27 1998-09-03 Cellomics, Inc. Systeme de criblage de cellules
WO2000003246A2 (fr) * 1998-07-13 2000-01-20 Cellomics, Inc. Systeme destine a un criblage a base de cellules
WO2000017643A2 (fr) * 1998-09-18 2000-03-30 Cellomics, Inc. Systeme de criblage cellulaire
WO2000050872A2 (fr) * 1999-02-26 2000-08-31 Cellomics, Inc. Systeme de criblage cellulaire
WO2000070342A2 (fr) * 1999-05-14 2000-11-23 Cellomics, Inc. Un systeme de criblage a base de cellules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HENDZEL MICHAEL J ET AL: "Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation.", CHROMOSOMA (BERLIN), vol. 106, no. 6, November 1997 (1997-11-01), pages 348 - 360, XP000980606, ISSN: 0009-5915 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8953866B2 (en) 2004-03-16 2015-02-10 Amnis Corporation Method for imaging and differential analysis of cells

Also Published As

Publication number Publication date
AU6622800A (en) 2001-03-05
WO2001011341A2 (fr) 2001-02-15

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