WO2001011061A3 - Regulation of embryonic transcription in plants - Google Patents

Regulation of embryonic transcription in plants Download PDF

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Publication number
WO2001011061A3
WO2001011061A3 PCT/CA2000/000907 CA0000907W WO0111061A3 WO 2001011061 A3 WO2001011061 A3 WO 2001011061A3 CA 0000907 W CA0000907 W CA 0000907W WO 0111061 A3 WO0111061 A3 WO 0111061A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
regulation
constructs
embryonic transcription
accumulation
Prior art date
Application number
PCT/CA2000/000907
Other languages
French (fr)
Other versions
WO2001011061A2 (en
Inventor
Ljerka Kunst
Sabine Clemens
Original Assignee
Univ British Columbia
Ljerka Kunst
Sabine Clemens
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ British Columbia, Ljerka Kunst, Sabine Clemens filed Critical Univ British Columbia
Priority to AU65513/00A priority Critical patent/AU6551300A/en
Priority to CA2345028A priority patent/CA2345028C/en
Priority to US09/806,708 priority patent/US6784342B1/en
Publication of WO2001011061A2 publication Critical patent/WO2001011061A2/en
Publication of WO2001011061A3 publication Critical patent/WO2001011061A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Nucleic acid constructs are provided comprising transcriptional regulatory regions homologous to plant FAE1 promoters. In some embodiments, these constructs may be used in transgenic cells or plants to promote expression of foreign and endogenous genes in developing seeds, for example to affect seed lipid metabolism, protein or carbohydrate composition and accumulation, or seed development.
PCT/CA2000/000907 1999-08-04 2000-08-04 Regulation of embryonic transcription in plants WO2001011061A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU65513/00A AU6551300A (en) 1999-08-04 2000-08-04 Regulation of embryonic transcription in plants
CA2345028A CA2345028C (en) 1999-08-04 2000-08-04 Regulation of embryonic transcription in plants
US09/806,708 US6784342B1 (en) 1999-08-04 2000-08-04 Regulation of embryonic transcription in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14713399P 1999-08-04 1999-08-04
US60/147,133 1999-08-04

Publications (2)

Publication Number Publication Date
WO2001011061A2 WO2001011061A2 (en) 2001-02-15
WO2001011061A3 true WO2001011061A3 (en) 2001-06-07

Family

ID=22520403

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2000/000907 WO2001011061A2 (en) 1999-08-04 2000-08-04 Regulation of embryonic transcription in plants

Country Status (3)

Country Link
AU (1) AU6551300A (en)
CA (1) CA2345028C (en)
WO (1) WO2001011061A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE383433T1 (en) 1999-08-26 2008-01-15 Calgene Llc PLANTS WITH MODIFIED POLYUNSATURATED FATTY ACIDS
US7531718B2 (en) 1999-08-26 2009-05-12 Monsanto Technology, L.L.C. Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
DE19950589A1 (en) * 1999-10-20 2001-05-23 Gvs Ges Fuer Erwerb Und Verwer Elongase promoters for tissue-specific expression of transgenes in plants
FR2808286B1 (en) * 2000-04-26 2004-12-17 Agronomique Inst Nat Rech NOVEL PLANT GLUCOSIDASE I AND ITS APPLICATION TO THE PRODUCTION OF RECOMBINANT PROTEINS WITH MODIFIED GLYCOSYLATION
US7253337B2 (en) * 2000-05-24 2007-08-07 The University Of British Columbia Gene regulatory region that promotes early seed-specific transcription
BR0111115A (en) * 2000-05-24 2003-04-08 Univ British Columbia nucleic acid encoding a very long chain fatty acid biosynthetic enzyme from plant
GB0031558D0 (en) * 2000-12-22 2001-02-07 Biogemma Uk Ltd Elongase promoters
EP1484959A4 (en) 2002-03-21 2005-08-31 Monsanto Technology Llc Nucleic acid constructs and methods for producing altered seed oil compositions
US7566813B2 (en) 2002-03-21 2009-07-28 Monsanto Technology, L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
US7166771B2 (en) 2002-06-21 2007-01-23 Monsanto Technology Llc Coordinated decrease and increase of gene expression of more than one gene using transgenic constructs
AU2003225283A1 (en) 2002-05-03 2003-11-17 Monsanto Technology, Llc Temporal seed promoters for expressing genes in plants
WO2007079353A2 (en) * 2005-12-15 2007-07-12 Targeted Growth, Inc. Increased seed size and seed number through transgenic over expression of a growth and/or development related gene during early embryo development
CN101421406B (en) 2006-02-13 2016-08-31 孟山都技术有限公司 For producing nucleic acid construct and the method that the seed oil of change forms
CN102164476A (en) 2008-09-29 2011-08-24 孟山都技术公司 Soybean transgenic event MON87705 and methods for detection thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015387A2 (en) * 1993-11-30 1995-06-08 Calgene, Inc. Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-coa metabolism
WO1996013582A1 (en) * 1994-10-26 1996-05-09 Dna Plant Technology Corporation Fae1 genes and their uses
WO1998046766A1 (en) * 1997-04-14 1998-10-22 The University Of British Columbia Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis
WO1998054954A1 (en) * 1997-06-03 1998-12-10 Cargill, Incorporated Fatty acid elongases
WO1999003983A1 (en) * 1997-07-18 1999-01-28 Carnegie Institution Of Washington Strong early seed-specific gene regulatory region
WO1999054471A1 (en) * 1998-04-20 1999-10-28 Zeneca Limited Polynucleotide sequences and their use in a method of producing plants with an increased number of stomata

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015387A2 (en) * 1993-11-30 1995-06-08 Calgene, Inc. Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-coa metabolism
WO1996013582A1 (en) * 1994-10-26 1996-05-09 Dna Plant Technology Corporation Fae1 genes and their uses
WO1998046766A1 (en) * 1997-04-14 1998-10-22 The University Of British Columbia Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis
WO1998054954A1 (en) * 1997-06-03 1998-12-10 Cargill, Incorporated Fatty acid elongases
WO1999003983A1 (en) * 1997-07-18 1999-01-28 Carnegie Institution Of Washington Strong early seed-specific gene regulatory region
WO1999054471A1 (en) * 1998-04-20 1999-10-28 Zeneca Limited Polynucleotide sequences and their use in a method of producing plants with an increased number of stomata

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL SEQUENCE DATABASE 4 July 1995 (1995-07-04), JAMES, D.W.; ET AL.: "DIRECTED TAGGING OF THE ARABIDOPSIS FATTY ACID ELONGATIONI (FAE1) GENE WITH THE MAIZE TRANSPOson ACTIVATOR", XP002163429 *
DATABASE EMBL SEQUENCE LIBRARY 24 July 1997 (1997-07-24), CLEMENS, S. AND KUNST, L.: "isolation f a Brassica napus cDNA (accession no. AF009563) encoding 3-ketoacyl-CoA synthase, a condensing enzyme involved in the biosynthesis of very long chain fatty acids in seeds (PGR97-125)", XP002163222 *
JAMES D W ET AL: "DIRECTED TAGGING OF THE ARABIDOPSIS FATTY ACID ELONGATIONI (FAE1) GENE WITH THE MAIZE TRANSPOREN ACTIVATOR", PLANT CELL,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 7, March 1995 (1995-03-01), pages 309 - 319, XP002911493, ISSN: 1040-4651 *
KUNST L ET AL: "FATTY ACID ELONGATION IN DEVELOPING SEEDS OF ARABIDOPSIS THALIANA", PLANT PHYSIOLOGY AND BIOCHEMISTRY,FR,GAUTHIER-VILLARS, PARIS, vol. 30, no. 4, 1992, pages 425 - 434, XP002063741, ISSN: 0981-9428 *
MILLAR ANTHONY A ET AL: "CUT1, an Arabidopsis gene required for cuticular wax biosynthesis and pollen fertility, encodes a very-long-chain fatty acid condensing enzyme.", PLANT CELL, vol. 11, no. 5, May 1999 (1999-05-01), pages 825 - 838, XP002163221, ISSN: 1040-4651 *
ROSCOE TJ ET AL: "Brassica napus fatty acid elongase (FAE1) homolog mRNA", EMBL NUCLEOTIDE SEQU, XP002109729 *
VENKATESWARI J ET AL: "Molecular cloning and characterization of FATTY ACID ELONGATION1 (BjFAE1) gene of Brassica juncea.", JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 8, no. 1, January 1999 (1999-01-01), pages 53 - 55, XP000991381, ISSN: 0971-7811 *

Also Published As

Publication number Publication date
AU6551300A (en) 2001-03-05
WO2001011061A2 (en) 2001-02-15
CA2345028A1 (en) 2001-02-15
CA2345028C (en) 2013-06-18

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