WO2001007042A1 - Apicidin-derived cyclic tetrapeptides - Google Patents
Apicidin-derived cyclic tetrapeptides Download PDFInfo
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- WO2001007042A1 WO2001007042A1 PCT/US2000/019627 US0019627W WO0107042A1 WO 2001007042 A1 WO2001007042 A1 WO 2001007042A1 US 0019627 W US0019627 W US 0019627W WO 0107042 A1 WO0107042 A1 WO 0107042A1
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- optionally substituted
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- heteroatoms
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- 0 CCC(C)[C@@](C(N(CCCC1)[C@]1C(N[C@@](CCCCCC(CC)=O)C(N[C@]1C2=CN(*)c3ccccc3C2=O)=O)=O)=O)NC1=O Chemical compound CCC(C)[C@@](C(N(CCCC1)[C@]1C(N[C@@](CCCCCC(CC)=O)C(N[C@]1C2=CN(*)c3ccccc3C2=O)=O)=O)=O)NC1=O 0.000 description 20
- ZZTFZBXRZUCQMB-UHFFFAOYSA-N CC(C1)C=Cc(c(C)c2)c1[n]2OC Chemical compound CC(C1)C=Cc(c(C)c2)c1[n]2OC ZZTFZBXRZUCQMB-UHFFFAOYSA-N 0.000 description 1
- HZWIJOWMGPTNRA-LAYXVCJNSA-N CCC(C)[C@@H](C(N(CCC1)[C@H]1C(N[C@@H](CCCCCC(CC)=O)C(N[C@H]1Cc(c2c3cccc2)c[n]3OC)=O)=O)=O)NC1=O Chemical compound CCC(C)[C@@H](C(N(CCC1)[C@H]1C(N[C@@H](CCCCCC(CC)=O)C(N[C@H]1Cc(c2c3cccc2)c[n]3OC)=O)=O)=O)NC1=O HZWIJOWMGPTNRA-LAYXVCJNSA-N 0.000 description 1
- RCKDICIRARVUDA-BDQQDBMKSA-N CCC(C)[C@@H](C(N(CCCC1)C1C(N[C@@H](CCCCCC(C(CO)O)=O)C(N[C@H]1Cc(c2c3cccc2)c[n]3OC)=O)=O)=O)NC1=O Chemical compound CCC(C)[C@@H](C(N(CCCC1)C1C(N[C@@H](CCCCCC(C(CO)O)=O)C(N[C@H]1Cc(c2c3cccc2)c[n]3OC)=O)=O)=O)NC1=O RCKDICIRARVUDA-BDQQDBMKSA-N 0.000 description 1
- YMXARZNZFKMCEE-GHTXXUPZSA-N CCC(C)[C@@H](C(N(CCCC1)[C@H]1C(N[C@@H](CCCCCC(CC)=O)C(N[C@H]1CC(c2ccccc2[N+]([O-])=O)=O)=O)O)=O)NC1=O Chemical compound CCC(C)[C@@H](C(N(CCCC1)[C@H]1C(N[C@@H](CCCCCC(CC)=O)C(N[C@H]1CC(c2ccccc2[N+]([O-])=O)=O)=O)O)=O)NC1=O YMXARZNZFKMCEE-GHTXXUPZSA-N 0.000 description 1
- MQODNRIMHZHAPM-RNHXBHQPSA-N CCC(C)[C@@H](C(N1C(C(N[C@@H](CCCCCC(CC)=O)C(N[C@@H]2C(O)=O)=O)=O)=CCCC1)=O)NC2=O Chemical compound CCC(C)[C@@H](C(N1C(C(N[C@@H](CCCCCC(CC)=O)C(N[C@@H]2C(O)=O)=O)=O)=CCCC1)=O)NC2=O MQODNRIMHZHAPM-RNHXBHQPSA-N 0.000 description 1
- GWLHFNKLJRZBAI-KGKLHAENSA-N CCC(CCCCC[C@@H](C(N[C@@H](Cc(c1c2cccc1)c[n]2OC)C(N[C@@H](C(C)C)C(N1[C@@H]2CCCC1)=O)=O)=O)NC2=O)=O Chemical compound CCC(CCCCC[C@@H](C(N[C@@H](Cc(c1c2cccc1)c[n]2OC)C(N[C@@H](C(C)C)C(N1[C@@H]2CCCC1)=O)=O)=O)NC2=O)=O GWLHFNKLJRZBAI-KGKLHAENSA-N 0.000 description 1
- MPQALXIIGYOFJW-KTLACUDISA-N CCCCCCCCCC(C(N[C@@H](Cc(c1c2cccc1)c[n]2S(C)(=O)=O)C(N[C@@H](C(C)CC)C(N1C2CCCC1)=O)=O)=O)NC2=O Chemical compound CCCCCCCCCC(C(N[C@@H](Cc(c1c2cccc1)c[n]2S(C)(=O)=O)C(N[C@@H](C(C)CC)C(N1C2CCCC1)=O)=O)=O)NC2=O MPQALXIIGYOFJW-KTLACUDISA-N 0.000 description 1
- LJIMZDNJQWBYLV-UHFFFAOYSA-N Cc(c1c2CCC=C1)c[n]2OC Chemical compound Cc(c1c2CCC=C1)c[n]2OC LJIMZDNJQWBYLV-UHFFFAOYSA-N 0.000 description 1
- WXPWZZHELZEVPO-UHFFFAOYSA-N Cc(cc1)ccc1C(c1ccccc1)=O Chemical compound Cc(cc1)ccc1C(c1ccccc1)=O WXPWZZHELZEVPO-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/12—Cyclic peptides with only normal peptide bonds in the ring
- C07K5/126—Tetrapeptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
- A61P33/06—Antimalarials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- the present invention relates to anti-protozoal agents.
- the present invention relates to cyclic tetrapeptide compounds derived from apicidin that therapeutically inhibit histone deacetylase activity by protozoa.
- Parasitic protozoa are responsible for a wide variety of infections in man and animals. Many of the diseases are life threatening to the host and cause considerable economic loss in animal husbandry. Malaria remains a significant health threat to humans despite massive international attempts to eradicate the disease. Trypanosomiasis such as i) Chagas disease caused by Trypanosoma cruzi and ii) African sleeping sickness caused by T. brucei are not uncommon in Africa and South America. Furthermore; opportunistic infections, caused by Pneumocystis carinii, Toxoplasma gondii, and Crypto sporidium sp., in immunocompromised hosts are becoming increasingly significant in developed countries.
- coccidiosis a widespread disease of domesticated animals produced by infections by protozoa of the genus Eimeria.
- Eimeria species include those in poultry, namely E. tenella, E. acervulina, E. necatrix, E. praecox, E. mitis, E. brunetti and E. maxima.
- Coccidiosis can cause high levels of morbidity and mortality in poultry, resulting in extreme economic losses.
- HDA hyperacetylated histones
- trichostatin A and trapoxin A have been reported as reversible and irreversible inhibitors, respectively, of mammalian HDA (see e.g., Yoshida et al., BioAssays, 17(5), 423-430 (1995)).
- Trichostatin A has also been reported to inhibit partially purified yeast HDA (Sanchez del Pino et al., Biochem. J., 303, 723-729 (1994)).
- Trichostatin A is an antifungal antibiotic and has been shown i) to have anti-trichomonal activity as well as cell differentiating activity in murine erythroleukemia cells, and ii) the ability to induce phenotypic reversion in ⁇ -transformed fibroblast cells (see e.g., U.S. Patent No. 4,218,478; Yoshida et al., BioAssays, 17(5), 423-430 (1995); and references cited therein).
- Trapoxin A a cyclic tetrapeptide, induces morphological reversion of v-szs-transformed NTH3T3 cells (Yoshida and Sugita, Jap. J. Cancer Res., 83(4), 324-328 (1992).
- HDA inhibition as a target for cancer research is described in Saito et al., Proc. Natl Acad. Sci. USA, 96, 4592-4597(1999); Bernardi et al., Amino Acids 6, 315-318 (1994); and R.E. Shute et al., J. Med. Chem. 30, 71-78 (1987).
- U.S. Patent No. 5,620,953 describes novel cyclic tetrapeptides, including apicidin.
- Apicidin [cycZ ⁇ (N-O-methyl-L-T ⁇ p-L-Ile-D-Pip-L-2-amino-8- oxo-decanoyl)] is a broad-spectrum an ti protozoal, antifungal and antineoplastic agent isolated from the fermentation culture of Fusarium fungus.
- the structure of apicidin is shown below:
- the present invention relates to novel cyclic tetrapeptides and pharmaceutical compositions containing the tetrapeptides.
- the invention also concerns a method for treating protozoal infections by administering to a host suffering from protozoal infection a therapeutically effective amount of a compound that inhibits histone deacetylase. Additionally, the invention relates to the use of known cyclic tetrapeptides to inhibit histone deacetylase activity and effective as antiprotozoal agents.
- This invention relates i) to new antiprotozoal, antifungal and antineoplastic agents related to apicidin, ii) to processes for preparation of such novel agents, iii) to compositions containing such novel agents, iv) to the use of such novel agents in the treatment of parasitic infections, including malaria, in human and animals and v) the use of such novel agents in treating cancer.
- the compounds of this invention can be used as cytostatic compounds, as agents in treating abnormal cell differentiation or proliferation, as agents against tumor growth, or as antimitotic agents for cancer chemotherapy.
- the present invention relates to a novel cyclic tetrapeptide represented by Formula I shown below:
- X is (1) -CH2-
- n (1) one, or
- Rl is (1) R7,
- C6alkyl)aryl CO2R b , or halogen, and each group may be saturated, partly unsaturated or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an R c substituent,
- each group may be saturated, partly unsaturated, or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an Rc substituent, and wherein the benzene/heterocycle fused ring is attached at any site to X or to the tetrapeptide, or
- each heterocyclic ring independently optionally substituted by 1 to 4 groups, each group independently is C ⁇ -C5alkyl, C2- Csalkenyl, C ⁇ -C5perfluoroalkyl, amino, oxo, thiono, C(O)NR c R d , cyano, CO2R b or halogen, wherein each heterocycle may be saturated, partly unsaturated or fully unsaturated, and wherein each heteroatom independently is oxygen, sulfur, or nitrogen, and the nitrogen optionally has an Rc substituent;
- R2 is (1) optionally substituted C2-Ci2alkyl
- aryl(C()-C5alkyl) wherein the aryl is optionally substituted with 1 to 3 groups, wherein each group independently is R', or
- each heteroatom independently is oxygen, sulfur or nitrogen, wherein the heterocycle is optionally substituted by 1 to 3 groups, wherein each group independently is Rf, and the heterocycle may be saturated or partly unsaturated;
- R3 each independently is
- R5 is (1) isopropyl, or
- R6 each independently is (1) o
- R7 is (1) hydrogen
- optionally substituted aryl wherein the optional substituents on the alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl and aryl are 1 to 4 groups, and each group independently is (a) Ci-Csalkyl,
- each heteroatom in either heterocyclic ring independently is oxygen, sulfur or nitrogen and the second heterocyclic ring is optionally substituted by 1 to 3 groups, each group independently is R ⁇ l, and each heterocycle independently may be saturated or partly unsaturated,
- R a is (1) hydrogen
- Ci-CiQalkyl optionally substituted Ci-CiQalkyl, (3) optionally substituted C3-C ⁇ oalkenyl,
- C5-C8cycloalkenyl optionally substituted C5-C8cycloalkenyl, wherein the optional substituents on the Ci-Cioalkyl, C3-C ⁇ oalkenyl, C3-C ⁇ oalkynyl, C ⁇ -C ⁇ oalkanoyl, C3-C ⁇ oalkenoyl, C3-C ⁇ oalkynoyl, aroyl, aryl, C3-C7cycloalkanoyl, C5-C7cycloalkenoyl, Ci-
- Cioalkylsulfonyl, C3-C8cycloalkyl and Cs-Cscycloalkenyl are from 1 to 10 groups, wherein each group independently is hydroxy, Ci- C6alkoxy, C3-C7cycloalkyl, aryl C ⁇ -C3alkoxy, NRXRx, CO2R b ,
- arylsulfonyl optionally substituted with 1 to 3 groups, wherein each group independently is C ⁇ -C5alkyl, Ci- C5perfluoroalkyl, nitro, halogen or cyano,
- R b is (1) H
- Ci-CiQalkyl optionally substituted Ci-CiQalkyl, (4) optionally substituted C3-C ⁇ oalkenyl,
- ( ) 5 to 9-membered heterocycle which may be saturated or partially unsaturated, containing from 1 to 4 heteroatoms, wherein each heteroatom independently is oxygen, sulfur or nitrogen, and the heterocycle is optionally substituted with 1 to 5 groups, wherein each group independently is R e ,
- R c and R d are independently selected from R b ; or R c and R d together with the N to which they are attached form a 3- to 10-membered ring containing 0 to 2 additional heteroatoms, each additional heteroatom independently being oxygen, nitrogen, or (O) n i substituted sulfur, wherein the ring is optionally substituted with 1 to 3 groups, wherein each group independently is Rg, hydroxy, thioxo, or oxo;
- R e is (1) halogen, (2) Ci-C7alkyl,
- optionally substituted aryl wherein the optional substituents are from 1 to 3 groups, wherein each group independently is halogen, C ⁇ -C6alkyl, C ⁇ -C6alkoxy, or hydroxy,
- R f is (1) Ci-C4alkyl
- N ⁇ 3 ⁇ 4 wherein ⁇ 3 and ⁇ are each independently hydrogen, C ⁇ -C5alkyl, or SO2R b ,
- N to which they are attached form a 3- to 7-membered ring containing 0 to 2 additional heteroatoms, wherein the additional heteroatoms independently are oxygen, nitrogen, or (O)mi substituted sulfur, wherein the ring is optionally substituted with 1 to 3 groups, wherein each group independently is R e or oxo, (13) (Co-C4alkyl)NO2,
- Co-C4aryl where the aryl group is optionally substituted from 1 to 4 groups, wherein each group independently is CO2R b , C(O)NR c R d , NO2, halogen, OC(O)R a , OR a or C ⁇ -C4alkyl;
- Rg and R n together with the N to which they are attached form a 3- to 7-membered ring containing 0 to 2 additional heteroatoms, wherein each additional heteroatom independently is oxygen, nitrogen, or (O)mi substituted sulfur, and the ring is optionally substituted with 1 to 3 groups, wherein each group independently is R e or oxo; or
- Rg and R n are each independently
- Ci-C ⁇ alkyl optionally substituted with hydroxy, amino, or CO2R 1 ,
- R 1 is (1) hydrogen, (2) C ⁇ -C3perfluoroalkyl,
- aryl optional substituents are from 1 to 3 groups, wherein each group independently is halogen, C ⁇ -C6alkyl, C ⁇ -C6alkoxy, or hydroxy;
- R is a C ⁇ -C4alkyl
- ni 0 to 2;
- the novel cyclic tetrapeptide of this invention includes a genus of compounds represented by Formula I or a pharmaceutically acceptable salt thereof wherein: X is (1) -CH2-,
- Rl is (1) R7,
- a 3- to 8-membered heterocycle containing 1 to 4 heteroatoms, optionally substituted by 1 to 4 groups, each group independently is Ci-C5alkyl, C2-C5alkenyl, Ci- C5perfluoroalkyl, NRCRd, 0 ⁇ o, thiono, ORa, S(O) n iR a (where ni 0, 1 or 2), C(O)Ra, C(O)NRCRd C yano, (Co-
- C6alkyl)aryl CO2R b , or halogen
- each group may be saturated, partly unsaturated or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an Rc substituent,
- each heterocycle may be saturated, partly unsaturated or fully unsaturated, and wherein each heteroatom independently is oxygen, sulfur, or nitrogen, and the nitrogen optionally has an Rc substituent.
- Rl is (1) R 7 ,
- a 3- to 8-membered heterocycle containing 1 to 4 heteroatoms, optionally substituted by 1 to 4 groups, each group independently is C ⁇ -C5alkyl, C2-C5alkenyl, Ci- C5perfluoroalkyl, NRCRd, 0 ⁇ o, thiono, OR a , S(O) n iR a (where ni 0, 1 or 2), C(O)R a , C(O)NRCRd cy ano, (Co
- C6alkyl)aryl CO2R b , or halogen
- each group may be saturated, partly unsaturated or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an Rc substituent,
- each group may be saturated, partly unsaturated, or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an Rc substituent, and wherein the benzene/heterocycle fused ring is attached at any site to X or to the tetrapeptide, or
- each heterocyclic ring independently optionally substituted by 1 to 4 groups, each group independently is C ⁇ -C5alkyl, C2- Csalkenyl, C ⁇ -C5perfluoroalkyl, amino, oxo, thiono, C(O)NRCRd, cyano, CO2R b or halogen, wherein each heterocycle may be saturated, partly unsaturated or fully unsaturated, and wherein each heteroatom independently is oxygen, sulfur, or nitrogen, and the nitrogen optionally has an Rc substituent; and R2 is (1) optionally substituted C2-Ci2alkyl, (2) optionally substituted C2-Ci2alkenyl,
- each heteroatom independently is oxygen, sulfur or nitrogen, wherein the heterocycle is optionally substituted by 1 to 3 groups, wherein each group independently is Rf, and the heterocycle may be saturated or partly unsaturated.
- X is (1) -CH2-
- a 3- to 8-membered heterocycle containing 1 to 4 heteroatoms, optionally substituted by 1 to 4 groups, each group independently is C ⁇ -C5alkyl, C2-C5alkenyl, Ci- C5perfluoroalkyl, NRCRd, 0 ⁇ o, thiono, ORa, S(O) n iR a (where ni 0, 1 or 2), C(O)Ra, C(O)NRCRd, cyano, (Co-
- C6alkyl)aryl CO2R b , or halogen
- each group may be saturated, partly unsaturated or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an Rc substituent,
- each heterocyclic ring independently optionally substituted by 1 to 4 groups, each group independently is Ci-C5alkyl, C2- C5alkenyl, Ci-C5perfluoroalkyl, amino, oxo, thiono, C(O)NRCRd, cyano, CO2R b or halogen, wherein each heterocycle may be saturated, partly unsaturated or fully unsaturated, and wherein each heteroatom independently is oxygen, sulfur, or nitrogen, and the nitrogen optionally has an Rc substituent.
- Rl is (1) R 7 ,
- (6) a 3- to 8-membered heterocycle containing 1 to 4 heteroatoms, optionally substituted by 1 to 4 groups, each group independently is C ⁇ -C5alkyl, C2-C5alkenyl, Ci-
- a benzene ring fused to a 4- to 8-membered heterocyclic ring with from 1 to 4 heteroatoms optionally substituted by 1 to 4 groups each independently is C ⁇ -C5alkyl, C2- Csalkenyl, Ci-C5perfluoroalkyl, amino, oxo, thiono, C(O)NRCRd ; cyano, CO2R b or halogen, each group may be saturated, partly unsaturated, or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an Rc substituent, and wherein the benzene/heterocycle fused ring is attached at any site to X or to the tetrapeptide, or (8) a 4- to 8-membered heterocyclic ring with from 1 to 4 heteroatoms fused to a second 4- to 8-membered heterocyclic ring with from 1 to 4 heteroatoms, each heterocyclic ring independently optionally optionally
- Ri is (1) R7,
- C6alkyl)aryl CO2R b , or halogen
- each group may be saturated, partly unsaturated or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an Rc substituent,
- each heterocyclic ring independently optionally substituted by 1 to 4 groups, each group independently is C ⁇ -C5alkyl, C2-
- each heterocycle may be saturated, partly unsaturated or fully unsaturated, and wherein each heteroatom independently is oxygen, sulfur, or nitrogen, and the nitrogen optionally has an Rc substituent;and R2 is (1) optionally substituted C2-Ci2alkyl,
- aryl(Co-C5alkyl) wherein the aryl is optionally substituted with 1 to 3 groups, wherein each group independently is R*, or (xx) a 3- to 6-membered heterocycle containing from
- each heteroatom independently is oxygen, sulfur or nitrogen, wherein the heterocycle is optionally substituted by 1 to 3 groups, wherein each group independently is Rf, and the heterocycle may be saturated or partly unsaturated.
- R a is (1) hydrogen
- C5-C6cycloalkenyl optionally substituted C5-C6cycloalkenyl, wherein the optional substituents on the C ⁇ -C6alkyl, C3-C6alkenyl, C2-C4alkanoyl, C3-C4alkenoyl, aroyl, aryl, C5-C6cycloalkanoyl, Ci- C4alkylsulfonyl, C5-C6cycloalkyl and C5-C6cycloalkenyl are from 1 to 10 groups, wherein each group independently is hydroxy, methoxy, aryl methoxy, NRXRx, CO2R b CONR c R d > or halogen,
- arylsulfonyl optionally substituted with 1 to 3 groups, wherein each group independently is methyl, CF3, nitro, halogen or cyano, or
- R a is (1) hydrogen, (2) optionally substituted C ⁇ -C6alkyl,
- C2-C4alkanoyl, C3-C4alkenoyl, aroyl, aryl, Cs-C ⁇ cycloalkanoyl, Ci- C4alkylsulfonyl, C5-C6cycloalkyl and Cs-C ⁇ cycloalkenyl are from 1 to 10 groups, wherein each group independently is hydroxy, methoxy, aryl methoxy, NRXRx, CO2R b , CONR c R d > or halogen, (12) CF3,
- arylsulfonyl optionally substituted with 1 to 3 groups, wherein each group independently is methyl, CF3, nitro, halogen or cyano, or
- X is (1) -CH2-
- each group may be saturated, partly unsaturated or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an R c substituent,
- a benzene ring fused to a 4- to 8-membered heterocyclic ring with from 1 to 4 heteroatoms optionally substituted by 1 to 4 groups each independently is C ⁇ -C5alkyl, C2- Csalkenyl, C ⁇ -C5perfluoroalkyl, amino, oxo, thiono, C(O)NRCRd 5 cyano, CO2R b or halogen, each group may be saturated, partly unsaturated, or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an R c substituent, and wherein the benzene/heterocycle fused ring is attached at any site to X or to the tetrapeptide, or (18) a 4- to 8-membered heterocyclic ring with from 1 to 4 heteroatoms fused to a second 4- to 8-membered heterocyclic ring with from 1 to 4 heteroatoms, each heterocyclic ring independently optional
- R a is (1) hydrogen
- C5-C6cycloalkenyl optionally substituted C5-C6cycloalkenyl, wherein the optional substituents on the Ci-C ⁇ alky], C3-C6alkenyl, C2-C4alkanoyl, C3-C4alkenoyl, aroyl, aryl, C5-C6cycloalkanoyl, Ci- C4alkylsulfonyl, C5-C6cycloalkyl and C5-C6cycloalkenyl are from 1 to 10 groups, wherein each group independently is hydroxy, methoxy, aryl methoxy, NRXRx, CO2R b , CONR c R ⁇ or halogen,
- arylsulfonyl optionally substituted with 1 to 3 groups, wherein each group independently is methyl, CF3, nitro, halogen or cyano, or
- a 5- or 6-membered heterocycle containing 1 to 3 heteroatoms, wherein each heteroatom is oxygen, sulfur or nitrogen, wherein the heterocycle is optionally substituted by 1 to 3 groups, wherein each group independently is methyl, CF3, NMe2, C(O)NR c R d , cyano, CO2R b or halogen, and wherein the heterocycle may be saturated or partly unsaturated;
- X is (1) -CH2-
- Rl is (1) R7,
- each group may be saturated, partly unsaturated or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an Rc substituent,
- each heterocyclic ring independently optionally substituted by 1 to 4 groups, each group independently is C ⁇ -C5alkyl, C2-
- each heterocycle may be saturated, partly unsaturated or fully unsaturated, and wherein each heteroatom independently is oxygen, sulfur, or nitrogen, and the nitrogen optionally has an R substituent.
- X is (1) -CH2-
- each group may be saturated, partly unsaturated or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an Rc substituent,
- each group may be saturated, partly unsaturated, or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an Rc substituent, and wherein the benzene/heterocycle fused ring is attached at any site to X or to the tetrapeptide, or
- each heterocyclic ring independently optionally substituted by 1 to 4 groups, each group independently is C ⁇ -C5alkyl, C2- Csalkenyl, C ⁇ -C5perfluoroalkyl, amino, oxo, thiono, C(O)NRCRd 7 cyano, CO2R b or halogen, wherein each heterocycle may be saturated, partly unsaturated or fully unsaturated, and wherein each heteroatom independently is oxygen, sulfur, or nitrogen, and the nitrogen optionally has an R substituent.
- R6 each independently is
- X is (1) -CH2-
- NR b S(O)niR7 wherein ni is from 0 to 2
- a 3- to 8-membered heterocycle containing 1 to 4 heteroatoms, optionally substituted by 1 to 4 groups, each group independently is Ci-Csalkyl, C2-C5alkenyl, Ci- C5perfluoroalkyl, NRCRd, 0 ⁇ o, thiono, ORa, S(O) n iR a (where ni 0, 1 or 2), C(O)Ra, C(O)NRcRd, cyano, (Co- C6alkyl)aryl, CO2R b , or halogen, and each group may be saturated, partly unsaturated or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an R c substituent, (17) a benzene ring fused to a 4- to 8-membered heterocyclic ring with from 1 to 4
- each heterocycle may be saturated, partly unsaturated or fully unsaturated, and wherein each heteroatom independently is oxygen, sulfur, or nitrogen, and the nitrogen optionally has an Rc substituent.
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein X is preferably -CH2-.
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein X is preferably -C(O)-.
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein X is preferably not present.
- R is preferably a 3- to 8-membered heterocycle containing 1 to 4 heteroatoms, optionally
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein Ri is preferably a benzene ring fused to a 4- to 8-membered heterocyclic ring with from 1 to 4 heteroatoms, optionally substituted by 1 to 4 groups each independently is C ⁇ -C5alkyl, C2-C5alkenyl, Ci- C5perfluoroalkyl, amino, oxo, thiono, C(O)NRCRd ) cyano, CO2R b or halogen, each group may be saturated, partly unsaturated, or fully unsaturated, wherein the heteroatoms are each independently oxygen, sulfur, or nitrogen, in which the nitrogen optionally has an R c substituent, and wherein the benzene/heterocycle fused ring is attached at any site to X or to the tetrapeptide
- Ri is preferably a benzene ring fused to a 4- to 8-membered heterocyclic ring with from 1 to
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein Ri is preferably a 4- to 8-membered heterocyclic ring with from 1 to 4 heteroatoms fused to a second 4- to 8-membered heterocyclic ring with from 1 to 4 heteroatoms, each heterocyclic ring independently optionally substituted by 1 to 4 groups, each group independently is C ⁇ -C5alkyl, C2- Csalkenyl, C ⁇ -C5perfluoroalkyl, amino, oxo, thiono, C(O)NRCRd 5 cyano, CO2R b or halogen, wherein each heterocycle may be saturated, partly unsaturated or fully unsaturated, and wherein each heteroatom independently is oxygen, sulfur, or nitrogen, and the nitrogen optionally has an R c substituent.
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is Example 2, 3a, 3b, 3d, 10,
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is Example 22a, 22b, 23a, 23b, 145, 146c, 146d, 146e, 146f, or 147:
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is Example 21a, 21b, 24a, 24b, 26, 27, 28, 29, 30, 32, 37, 39, 43, 44, 46, 51, 56a, 63, 64, or 67:
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is Example 69, 70, 72, 73, 74a. 74b, 74c, 74d, 74e, 74f, 74g, 74h, 74i, 74j, 75, 79, 91, 93, 97, 98, 129a, or 129b:
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is Example 132a, 133, 135, 138, 139a, 139b, 139c, 139d, 139e, 139f, 139g, 139h, 139i, 139j, 140, 141, 142, 144b, 144d, 144f, 158, 159, 160, 162a, or 162b.
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is Example 102, 103, 108a, or 108b.
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is Example 109 or 110.
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is Example 168. In another aspect, the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is Example 156, 157a, 157b, 157c, or 157d.
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is
- the present invention provides a novel cyclic tetrapeptide represented by Formula I, wherein the compound is Example 153 or 154.
- the present invention provides a method for the treatment of protozoal infections comprising the step of administering to a host suffering from a protozoal infection a therapeutically effective amount of the novel compounds of the invention which inhibits histone deacetylase.
- a therapeutically effective amount is that safe amount sufficient to inhibit histone deacetylase activity of the causative protozoa to control and overcome the infection.
- the present invention also provides a method for the prevention of protozoal infections comprising the step of administering to a host an effective preventative amount of the novel compounds of the invention, which inhibits histone deacetylase.
- An effective preventative amount is that safe amount sufficient to inhibit the infection of the host.
- the present invention provides a composition useful for the treatment or prevention of protozoal diseases which comprises an inert carrier and an effective amount of a compound of formula I.
- alkyl as well as other groups having the prefix “alk” such as, for example, alkoxy, alkanoyl, alkenyl, alkynyl and the like, means carbon chains which may be linear or branched or combinations thereof.
- alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, sec- and tert-butyl, pentyl, hexyl, heptyl and the like.
- alkenyl alkynyl and other like terms include carbon chains containing at least one unsaturated C-C bond.
- cycloalkyl means carbocycles containing no heteroatoms, and includes mono-, bi- and tricyclic saturated carbocycles, as well as fused ring systems.
- fused ring systems can include one ring that is partially or fully unsaturated such as a benzene ring to form fused ring systems such as benzofused carbocycles.
- Cycloalkyl includes such fused ring systems as spirofused ring systems.
- cycloalkyl examples include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, decahydronaphthalene, adamantane, indanyl, indenyl, fluorenyl, 1,2,3,4- tetrahydronaphalene and the like.
- cycloalkenyl means carbocycles containing no heteroatoms and at least one non-aromatic C-C double bond, and include mono-, bi- and tricyclic partially saturated carbocycles, as well as benzofused cycloalkenes.
- cycloalkenyl examples include cyclohexenyl, indenyl, and the like.
- halogen includes fluorine, chlorine, bromine and iodine atoms.
- heterocycle means cyclic systems such as those described above for cycloalkyl and cycloalkenyl in which at least one atom is a sulfur, oxygen or nitrogen atom in a group of atoms that form the backbone of a ring.
- Such heterocycles include mono- or bicyclic compounds that are saturated or partly unsaturated, as well as benzo- or heteroaromatic ring fused saturated heterocycles or partly unsaturated heterocycles, and containing from 1 to 4 heteroatoms independently selected from oxygen, sulfur and nitrogen.
- saturated heterocycles include morpholine, thiomorpholine, piperidine, piperazine, tetrahydropyran, tetrahydrofuran, dioxane, tetrahydrothiophene, oxazolidine, pyrrolidine;
- partly unsaturated heterocycles include dihydropyran, dihydropyridazine, dihydrofuran, dihydrooxazole, dihydropyrazole, dihydropyridine, dihydropyridazine and the like.
- benzo- or heteroaromatic ring fused heterocycle examples include 2,3-dihydrobenzofuranyl, benzopyranyl, tetrahydroquinoline, tetrahydroisoquinoline, benzomorpholinyl, 1,4-benzodioxanyl, 2,3-dihydrofuro(2,3- b)pyridyl and the like.
- aryl is intended to include mono- and bicyclic aromatic and heteroaromatic rings containing from 0 to 5 heteroatoms independently selected from nitrogen, oxygen and sulfur.
- aryl is also meant to include benzofused cycloalkyl, benzofused cycloalkenyl, and benzofused heterocyclic groups.
- aryl groups include phenyl, pyrrolyl, isoxazolyl, pyrazinyl, pyridinyl, oxazolyl, thiazolyl, imidazolyl, triazolyl, tetrazolyl, furanyl, triazinyl, thienyl, pyrimidinyl, pyridazinyl, pyrazinyl, naphthyl, benzoxazolyl, benzothiazolyl, benzimidazolyl, benzofuranyl, furo(2,3-B)pyridyl, 2,3-dihydrofuro(2,3-b)pyridyl, benzoxazinyl, benzothiophenyl, quinolinyl, indolyl, 2,3-dihydrobenzofuranyl, benzopyranyl, 1,4- benzodioxanyl, indanyl, indenyl, fluorenyl,
- Aroyl means arylcarbonyl in which aryl is as defined above.
- NR c Rd or NR ⁇ R n forming a 3- to 10- membered ring containing 0 to 2 additional heteroatoms selected from O, S(O) m and N are aziridine, azetidine, pyrrolidine, piperidine, thiomorpholine, morpholine, piperazine, octahydroindole, tetrahydroisoquinoline and the like.
- Co means that the carbon is not present.
- C0-C5 means that there are from none to five carbons present - that is, five, four, three, two, one, or no carbons present.
- optionally substituted is intended to include both substituted and unsubstituted.
- optionally substituted aryl could represent a pentafluorophenyl or a phenyl ring.
- Compounds described herein contain one or more asymmetric centers and may thus give rise to diastereomers and optical isomers.
- the present invention includes all such possible diastereomers as well as their racemic mixtures, their substantially pure resolved enantiomers, all possible geometric isomers, and pharmaceutically acceptable salts thereof.
- the above Formula I is shown without a definitive stereochemistry at certain positions.
- the present invention includes all stereoisomers of Formula I. Further, mixtures of stereoisomers as well as isolated specific stereoisomers are also included. During the the course of the synthetic procedures used to prepare such compounds, or in using racemization or epimerization procedures known to those skilled in the art, the products of such procedures can be a mixture of stereoisomers.
- salts refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids.
- the compound of the present invention is acidic, its corresponding salt can be conveniently prepared from pharmaceutically acceptable non-toxic bases, including inorganic bases and organic bases.
- Salts derived from such inorganic bases include aluminum, ammonium, calcium, copper (ic and ous), ferric, ferrous, lithium, magnesium, manganese (ic and ous), potassium, sodium, zinc and the like salts. Particularly preferred are the ammonium, calcium, magnesium, potassium and sodium salts.
- Salts derived from pharmaceutically acceptable organic non-toxic bases include salts of primary, secondary, and tertiary amines, as well as cyclic amines and substituted amines such as naturally occurring and synthesized substituted amines.
- Other pharmaceutically acceptable organic non-toxic bases from which salts can be formed include ion exchange resins such as, for example, arginine, betaine, caffeine, choline, N,N -dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2- dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N- ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine,
- the compound of the present invention When the compound of the present invention is basic, its corresponding salt can be conveniently prepared from pharmaceutically acceptable non-toxic acids, including inorganic and organic acids.
- acids include, for example, acetic, benzenesulfonic, benzoic, camphorsulfonic, citric, ethanesulfonic, fumaric, gluconic, glutamic, hydrobromic, hydrochloric, isethionic, lactic, maleic, malic, mandelic, methanesulfonic, mucic, nitric, pamoic, pantothenic, phosphoric, succinic, sulfuric, tartaric, p-toluenesulfonic acid and the like.
- citric, hydrobromic, hydrochloric, maleic, phosphoric, sulfuric, and tartaric acids are particularly preferred.
- histone deacetylase inhibitors are described in pending U.S. Patent Applications 09/296,834, filed April 22, 1999, and 08/716,978, filed September 20, 1996.
- Known compounds that may be histone deacetylase inhibitors and therefore useful in the treatment of protozoal diseases include, for example, trichostatin A, trapoxin A and B, HC -toxin, chlamydocin, Cly-2, WF-3161, Tan-1746, apicidin, and analogs thereof.
- Trapoxin A is described in Itazaki et al., J. Antibiot. 43, 1524-1532(1990); HC-Toxin is described in Liesch et al., Tetrahedron 38 ⁇ 45-48(1982); chlamydocin is described in Closse et al., Helv. Chim. Acta 57, 533-545(1974); Cly-2 is described in Hirota et al., Agri. Biol. Chem 37, 955-56(1973); WF-3161 is described in Umehana et al., J. Antibiot. 36, 478-483(1983); and Tan-1746 is described in Japanese Patent No. 7196686.
- HC toxin, chlamydocin, trapoxin A and trapoxin B contain a C8 ⁇ -ketoepoxide functionality.
- Apicidin and analogs thereof referred to herein are described by the following chemical formula:
- the compounds are described in pending U.S. Patent Application Nos. 08/281,325, filed July 27, 1994 and 08/447,664, filed May 23, 1995.
- the compounds are produced from a strain of Fusa ⁇ um as disclosed in the applications.
- the compounds of the present invention have been found to be histone deacetylase inhibitors. Accordingly, they can be useful in the treatment and prevention of protozoal diseases in human and animals, including poultry.
- protozoal diseases against which histone deacetylase inhibitors may be used include: 1) amoebiasis (Dientamoeba sp., Entamoeba histolytica); 2) giardiasis (Giardia lamblia); 3) malaria (Plasmodium species including P. vivax, P. falciparum, P. malariae and P. ovale); 4) leishmaniasis (Leishmania species including L. donovani, L. tropica, L. mexicana, and L. braziliensis); 5) trypanosomiasis and Chagas disease (Trypanosoma species including T. brucei, T.
- vaginitis Trichomonas species including T.vaginitis, and T.
- coccidiosis Eimeria species including E. tenella, E. necatrix, E. acervulina, E. maxima and E. brunetti, E. mitis, E. bovis, E. melagramatis, and Isospora sp ; 13) enterohepatitis (Histomonas gallinarum); and 14) infections caused by Anaplasma sp., Besnoitia sp., Leucocytozoan sp., Microspo ⁇ dia sp., Sarcocystis sp., Theileria sp., and Pneumocystis carinii.
- Anaplasma sp. Besnoitia sp., Leucocytozoan sp., Microspo ⁇ dia sp., Sarcocystis sp., Theileria sp., and Pneumocystis carinii.
- the histone deacetylase inhibiting compounds and compositions of the present invention are preferably used in the treatment or prevention of protozoal infections caused by a member of the sub-phylum Apicomplexans. More preferably the compounds and compositions are used i) in the treatment or prevention of malaria, toxoplasmosis, cryptosporidiosis and trypanosomiasis in humans and animals, and ii) in the management of coccidiosis, particularly in poultry, either to treat coccidial infection or to prevent the occurrence of such infection.
- the histone deacetylase inhibitor When the histone deacetylase inhibiting compounds and compositions of this invention are administered on a chronic basis, such as in the prevention of coccidiosis in poultry, the histone deacetylase inhibitor preferably is selective for protozoal histone deacetylase over the host histone deacetylase. Such a selective inhibitor would minimize adverse histone deacetylase inhibition effects to the host over the long term.
- Two specific examples of the method of this invention of administering an effective preventative amount of an histone deacetylase inhibitor to prevent the establishment of parasitic infections in humans and animals are 1) the prevention of Plasmodium (malaria) infection in humans in endemic areas and 2) the prevention of coccidiosis in poultry.
- the histone deacetylase-inhibiting compound can be conveniently administered continually in the feed or drinking water, or regularly by oral or parenteral dosing. Malaria is the number one cause of death in the world. The disease is transmitted by mosquitoes in endemic areas and can very rapidly progress to a life threatening infection. Therefore, individuals living in or visiting areas where malaria carrying mosquitoes are present routinely take prophylactic drugs to prevent infection.
- a histone deacetylase inhibitor is administered orally or parenterally one or more time(s) a day, preferably each dose ranges from about O.Olmg/kg to about lOOmg/kg.
- the compound may be administered for the entire period during which the patient or animal is at risk of acquiring a parasitic infection.
- Coccidiosis is a disease that can occur in humans and animals and is caused by several genera of coccidia. The most economically important occurrence of coccidiosis is the disease in poultry. Coccidiosis in poultry is caused by protozoan parasites of the genus Eimeria. The disease can spread quite rapidly throughout flocks of birds via contaminated feces. The parasites destroy gut tissue and damage the gut lining, thereby impairing nutrient absorption. An outbreak of coccidiosis in a poultry house can cause such dramatic economic losses for poultry producers that it has become standard practice to use anticoccidial agents prophylactically in the poultry feed.
- a histone deacetylase inhibitor is administered in the feed or drinking water for the entire or a portion of the lifetime of domestic birds with a dose that ranges between about 0.1 ppm to about 500ppm in the feed or water.
- the histone deacetylase inhibitor is conveniently administered orally or parenterally when the infection is suspected or diagnosed.
- the treatment period varies according to the specific parasitic disease and the severity of the infection. In general the treatment is continued until the parasites are effectively eradicated and/or the symptoms of the disease are resolved.
- Two specific examples of the method of this invention for the treatment of protozoal infections by administering a therapeutically effective amount of a histone deacetylase inhibitor are 1) the treatment of a Cryptosporidium parvum infection in an animal or human and 2) the treatment of acute Plasmodium falciparum malaria in humans.
- Cryptosporidium parvum is a protozoan parasite that infects and destroys cells lining the intestinal tract of humans and animals. The infection establishes quite rapidly and has acute effects on the patient. In the case of humans, patients get severe dysentery for a period of 5-7 days. In immune compromised patients C. parvum infections can persist and can be life threatening. In animals C. parvum infection is the leading cause of death in young dairy calves. A C. parvum infection can be easily diagnosed by symptoms and examination of a stool sample. When the disease is suspected and/or diagnosed, treatment with a histone deacetylase inhibitor according to the method of this invention can be initiated. The dose preferably ranges from about O.Olmg/kg to about 500mg/kg. The histone deacetylase is administered one or more time(s) a day, orally or parenterally until the infection is eliminated. The dosing period typically is in the range of about 1-3 weeks.
- P. falciparum causes acute life threatening malarial infections in humans.
- the infection if left untreated can often result in the death of the patient.
- a malaria infection can be easily diagnosed by symptoms and examination of a blood sample from the patient. Treatment would be initiated following diagnosis.
- a histone deacetylase inhibitor is administered one or more time(s) a day, orally or parenterally, until the infection is eliminated.
- the dose preferably ranges from about O.Olmg kg to about 200 mg/kg.
- the histone deacetylase inhibiting compositions of this invention may be administered to a host in need of treatment in a manner similar to that used for other known antiprotozoal agents.
- the compositions may be administered parenterally, orally, topically, or rectally.
- the dosage to be administered will vary according to the particular compound used, the infectious organism involved, the particular host, the severity of the disease, the physical condition of the host, and the selected route of administration; the appropriate dosage can be readily determined by a person skilled in the art.
- the dosage preferably ranges from about O.Olmg/kg to about 500mg/kg.
- the dosage preferably ranges from about O.Olmg/kg to about lOOmg/kg.
- the compound is preferably administered in the animals' feed or drinking water.
- the dosage preferably ranges from about O.lppm to about 500ppm.
- the composition of the present invention comprises a histone deacetylase inhibitor and an inert carrier.
- the compositions include pharmaceutical compositions for human and veterinary usage, and feed compositions for the control of coccidiosis in poultry.
- compositions of the present invention comprise a histone deacetylase inhibitor as an active ingredient, a pharmaceutically acceptable carrier and optionally other therapeutic ingredients or adjuvants.
- the compositions include compositions suitable for oral, rectal, topical, and parenteral (including subcutaneous, intramuscular, and intravenous) administration, although the most suitable route in any given case will depend on the particular host, and nature and severity of the conditions for which the active ingredient is being administered.
- the pharmaceutical compositions may be conveniently presented in unit dosage form and prepared by any of the methods well known in the art of pharmacy.
- the histone deacetylase inhibitor of this invention can be combined as the active ingredient in intimate admixture with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques.
- the carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral (including intravenous).
- the pharmaceutical compositions of the present invention can be presented as discrete units suitable for oral administration such as capsules, cachets or tablets each containing a predetermined amount of the active ingredient.
- compositions can be presented as a powder, as granules, as a solution, as a suspension in an aqueous liquid, as a non-aqueous liquid, as an oil-in-water emulsion or as a water-in-oil liquid emulsion.
- the histone deacetylase inhibitors may also be administered by controlled release means and/or delivery devices.
- the compositions may be prepared by any of the methods of pharmacy. In general, such methods include a step of bringing into association the active ingredient with the carrier that constitutes one or more necessary ingredients. In general, the compositions are prepared by uniformly and intimately admixing the active ingredient with liquid carriers or finely divided solid carriers or both.
- any convenient pharmaceutical media may be employed.
- water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents and the like may be used to form oral liquid preparations such as suspensions, elixirs and solutions; while carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents, and the like may be used to form oral solid preparations such as powders, capsules and tablets. Because of their ease of administration, tablets and capsules are the preferred oral dosage units whereby solid pharmaceutical carriers are employed.
- tablets may be coated by standard aqueous or nonaqueous techniques
- a tablet containing the composition of this invention may be prepared by compression or molding, optionally with one or more accessory ingredients or adjuvants.
- Compressed tablets may be prepared by compressing, in a suitable machine, the active ingredient in a free-flowing form such as powder or granules, optionally mixed with a binder, lubricant, inert diluent, surface active or dispersing agent. Molded tablets may be made by molding in a suitable machine, a mixture of the powdered compound moistened with an inert liquid diluent.
- Each tablet preferably contains from about lmg to about 500mg of the active ingredient and each cachet or capsule preferably containing from about 1 to about 500mg of the active ingredient.
- Pharmaceutical compositions of the present invention suitable for parenteral administration may be prepared as solutions or suspensions of the active compounds in water.
- a suitable surfactant can be included such as, for example, hydroxypropylcellulose.
- Dispersions can also be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof in oils. Further, a preservative can be included to prevent the detrimental growth of microorganisms.
- compositions of the present invention suitable for injectable use include sterile aqueous solutions or dispersions.
- the compositions can be in the form of sterile powders for the extemporaneous preparation of such sterile injectable solutions or dispersions.
- the final injectable form must be sterile and must be effectively fluid for easy syringability.
- the pharmaceutical compositions must be stable under the conditions of manufacture and storage; thus, preferably should be preserved against the contaminating action of microorganisms such as bacteria and fungi.
- the carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g. glycerol, propylene glycol and liquid polyethylene glycol), vegetable oils, and suitable mixtures thereof.
- Pharmaceutical compositions of the present invention can be in a form suitable for topical use such as, for example, an aerosol, cream, ointment, lotion, dusting powder, or the like. Further, the compositions can be in a form suitable for use in transdermal devices.
- compositions may be prepared, utilizing the histone deacetylase inhibiting compounds of this invention, via conventional processing methods.
- a cream or ointment is prepared by mixing hydrophilic material and water, together with about 5 wt% to about 10 wt% of the compound, to produce a cream or ointment having a desired consistency.
- compositions of this invention can be in a form suitable for rectal administration wherein the carrier is a solid. It is preferable that the mixture forms unit dose suppositories.
- Suitable carriers include cocoa butter and other materials commonly used in the art.
- the suppositories may be conveniently formed by first admixing the composition with the softened or melted carrier(s) followed by chilling and shaping in moulds.
- the pharmaceutical formulations described above may include, as appropriate, one or more additional carrier ingredients such as diluents, buffers, flavoring agents, binders, surface-active agents, thickeners, lubricants, preservatives (including anti-oxidants) and the like.
- additional carrier ingredients such as diluents, buffers, flavoring agents, binders, surface-active agents, thickeners, lubricants, preservatives (including anti-oxidants) and the like.
- other adjuvants can be included to render the formulation isotonic with the blood of the intended recipient.
- the histone deacetylase inhibitor of this invention can be conveniently administered as a component of a feed composition.
- the poultry feed preferably contains from about lppm to about lOOOppm, more preferably from about lOppm to about 150ppm of the histone deacetylase inhibitor of this invention.
- the optimum levels will vary with the species of Eimeria involved, and can be readily determined by one skilled in the art. It is preferred that the histone deacetylase inhibitor of this invention be added to poultry feed in the amount of from about 0.01% to about 0.1% by weight of the diet.
- the compositions of this invention are especially useful in controlling the pathology associated with E. tenella.
- the preferred concentration for similar control of intestinal-dwelling species is from about 0.01% to about 0.1% by weight of the diet. Amounts of about 0.01% to about 0.1% percent by weight are advantageous in reducing the pathogenic effects of both fecal coccidiosis and intestinal coccidiosis.
- the histone deacetylase inhibitor can be conveniently dispersed, for example, by i) being mechanically mixed in a finely ground form with the poultry feedstuff, or ii) being first mixed with an intermediate formulation (to form a premix) that is subsequently blended with other poultry feedstuff components.
- Typical components of poultry feedstuffs include molasses, fermentation residues, corn meal, ground and rolled oats, wheat shorts and middlings, alfalfa, clover and meat scraps, together with mineral supplements such as bone meal, calcium carbonate and vitamins.
- compositions containing a compound described by formula I may also be prepared in powder or liquid concentrate form.
- conventional water-soluble excipients such as lactose or sucrose, may be incorporated in the powders to improve their physical properties.
- powder compositions of this invention comprise from about
- powders may either be added to animal feedstuffs, for example, by way of an intermediate premix, or added to the animal drinking water by dilution.
- Liquid concentrates of this invention suitably contain a water-soluble compound combination and may optionally further include a veterinary acceptable water miscible solvent.
- a solvent such as polyethylene glycol, propylene glycol, glycerol, or glycerol formal can be mixed with up to 30% v/v of ethanol. It is preferable that the liquid concentrates of this invention comprise from about 50wt% to about 100wt%, and more preferably about 60wt% to about 80wt% of the compound.
- the liquid concentrates may be administered to the drinking water of animals, particularly poultry.
- apicidin can be converted into alpha- substituted analog compounds 4 and 5.
- Apicidin is first enolized with an appropriate amine base including, but not limited to, LiN( Pr)2, NaN(SiMe3)2, KN(SiMe3)2, and the like at temperatures ranging from -78°C to 0°C to form an enolate.
- the amine base is preferably KN(SiMe3)2-
- Appropriate solvents for this reaction include, but are not limited to, Et2 ⁇ , dioxane, tetrahydrofuran (THF), dimethoxyethane, and the like.
- the solvent is preferably THF.
- the enolate is reacted with an appropriate electrophile RX including, but not limited to, Mel, Etl, allyl bromide, benzyl bromide, PhSeCl, PhSCl, PhSSPh, (MeO)2P(O)Cl, (CF2SO2)2O, Et3SiCl, tBu(Me)2SiCl, (nPr)3SiCl, Me3SiCl, Ph(Me)2SiCl, and the like to form a silyl enol ether.
- the electrophile is preferably Me3SiCl.
- silyl enol ethers Treatment of the thus prepared silyl enol ethers with an oxidant, including but not limited to, H2O2, tBuOOH, Me3SiOOH, AcOOH, dimethydioxirane and the like, or preferably MCPBA (meta-chloroperbenzoic acid), at temperatures from -78°C to RT (room temperature) but preferably 0°C to RT will produce the corresponding alpha-silyloxyketones, compounds 4a/5a.
- an oxidant including but not limited to, H2O2, tBuOOH, Me3SiOOH, AcOOH, dimethydioxirane and the like, or preferably MCPBA (meta-chloroperbenzoic acid)
- the silyl protecting groups can be then removed using a variety of acid or fluoride sources including, but not restricted to, HC1, H2SO4, HBF4, acetic acid, PPTS (pyridinium p- toluenesulfonate), TsOH (p-toluenesulfonyl hydroxide), HF, HF'pyridine, or Bu4NF and the like in protic or aprotic solvents including, but not limited to, CH2CI2, CHCI3, MeOH, EtOH, iPrOH, THF, Et2 ⁇ and dioxane and the like at temperatures from 0°C to 50°C to generate the alpha-hydroxyketones, compounds 4d/5d.
- acid or fluoride sources including, but not restricted to, HC1, H2SO4, HBF4, acetic acid, PPTS (pyridinium p- toluenesulfonate), TsOH (p-toluenesulfonyl hydroxide),
- the alpha-hydroxyketone compounds 4d/5d may be separated or used with no further separation, as desired.
- Compounds 4d/5d can be oxidized to the corresponding diketones, compounds 4e/5e, by treatment including, but not limited to, Swern oxidation, Dess-Martin oxidation, PCC (pyridinium chlorochromate), PDC (pyridinium dichromate), Moffat-oxidation, and the like, or most preferably TPAP/NMO (tetrapropylammonium perruthenate(VU)/4-methylmo holine N-oxide) in solvents including, but not limited to, toluene, CH2CI2, CHCI3 and the like at temperatures ranging from -78°C to RT.
- TPAP/NMO tetrapropylammonium perruthenate(VU)/4-methylmo holine N-oxide
- the alpha-hydroxyketone compounds 4d/5d can be converted into the corresponding alpha-haloketone compounds such as 4f/5f by treatment with Ph3P/CBr4, Ph3P/l2, PH3P/CCI4, Ph3P/CHCl2CHCl2, DAST (diethylaminosulfur trifluoride), morpholinyl sulfur trifluoride, and the like in solvents such as CH2CI2, CHCI3, benzene, toluene and the like at temperatures from -78°C to RT.
- the alpha-hydroxyketone compounds 4d/5d can be treated with an oxidizing agent including, but not restricted to, NaK>4, HIO4, Mn ⁇ 2, Amberlite®
- IRA-904 ion-exchange resin available from Aldrich Chemical Company, Milwaukee, Wisconsin, NaIO4, KIO4, and nBu4NIO4, or most preferably Pb(OAc)4 to yield a
- the oxidative cleavage reaction may be performed in a variety of solvents or mixtures of solvents, including water, EtOH, ⁇ ' PrOH (isopropanol), tBuOH (tert-butanol), acetone, ether, THF, benzene, toluene, CH2CI2, CHCI3, and the like, or most preferably MeOH.
- the oxidative cleavage reaction is performed at temperatures from about -78°C to about 80°C. When utilizing MeOH, the reaction should be performed at temperatures from -20°C to RT.
- the oxidative cleavage reaction may be improved by the addition of a base, including but not restricted to NaHCO3, Et3N, EtN( Pr)2, lutidine and the like, or most preferably pyridine.
- a base including but not restricted to NaHCO3, Et3N, EtN( Pr)2, lutidine and the like, or most preferably pyridine.
- the oxidative cleavage reaction is generally complete in from about 5 minutes to about 24 hours.
- the phenylsulfide compounds 4c/5c or phenylselenide compounds 4b/5b, analogs of apicidin are oxidized to the corresponding sulfoxide or selenoxide compounds (not shown) using reagents which include, but not limited to, Oxone, MCPBA, tBuOOH, AcOOH, NaIO4, dimethyldioxirane, and the like, or most preferably H2O2, in solvents or mixtures of solvents, including, but not limited to toluene, CHCI3, MeOH, water, or most preferably CH2CI2 and at temperatures ranging from -20°C to 50°C.
- reagents include, but not limited to, Oxone, MCPBA, tBuOOH, AcOOH, NaIO4, dimethyldioxirane, and the like, or most preferably H2O2, in solvents or mixtures of solvents, including, but not limited to toluene, CHCI3, MeOH, water, or most preferably CH
- Enone compounds 8 and 9 can be epoxidized (not shown) with appropriate epoxidizing agents including, but not limited to, dimethyldioxirane,
- Enone compounds 8 and 9 also may be dihydroxylated with OSO4 under conditions known to those skilled in the art to form the corresponding diols.
- Osmium tetroxide may be used either stoichiometrically or catalytically in the presence of an oxidant including, but not restricted to, morpholine N-oxide, trimethylamine N-oxide, hydrogen peroxide, tert-butyl hydroperoxide and the like.
- the dihydroxylation reactions are performed in a variety of solvents or mixtures of solvents.
- the solvents include both protic and aprotic solvents such as water, MeOH, EtOH, tert-butanol, ether, THF, benzene, pyridine, acetone, and the like.
- the dihydroxylation reactions are performed at from -78 °C to 80°C and are complete in from 5 minutes to 24 hours.
- the diol products thus obtained can be oxidatively cleaved as described previously for compounds 6 and 7 to yield a C6-aldehyde compound 10 and a C8 methyl ester compound 7 from compounds 8 and 9, respectively.
- apicidin's sidechain C8-ketone group can be a starting point for analog synthesis.
- Rllb Rile Rlld, Rllfl, Rllf2.
- Rllg, RHh, Rllil, Rlli2, and Rllk are each independently an alkyl or aryl group which optionally is substituted.
- the sidechain C8-ketone group can be reduced using reagents known to those skilled in the art, including, but not limited to LiBI j., IJAIH4, DIBAL-H (diisobutylaluminum hydride), K-Selectride® (potassium t ⁇ -sec- butylborohydride) available from Aldrich Chemical Company, Milwaukee, Wisconsin, L-Selectride® (lithium tri-sec-butylborohydride) available from Aldrich, Alpine-Borane® (B-isopinocampheyl-9-borabicyclo[3.3.1]-nonane) available from Aldrich, and the like or most preferably NaBH j.
- LiBI j. LiBI j.
- DIBAL-H diisobutylaluminum hydride
- K-Selectride® potassium t ⁇ -sec- butylborohydride
- L-Selectride® lithium tri-sec-butylborohydr
- Apicidin 's sidechain C8-ketone group can also be treated with RMgBr, RMgCl, RMgl, RLi, R2CuLi, RCeCl2Li and the like to generate substituted alcohol compounds lib.
- R is an alkyl or aryl group, and the alkyl and aryl groups are optionally substituted.
- substitution reactions may be performed in solvents or mixtures of solvents, including but not limited to, Et2 ⁇ , dioxane, HMPA (hexamethylphosphoramide), DMSO, NMP (1- methyl-2-pyrrolidinone), dimethoxyethane, and the like, or most preferably THF, at temperatures from -78 °C to RT, and are complete in from 5 minutes to 12 hours.
- the C8-alcohol compound 11a generated above can be alkylated, acylated or sulfonylated using known methods for acylation, sulfonylation and alkylation of alcohols to generate apicidin derivative compounds lie or lid.
- acylation may be accomplished using reagents such as acid anhydrides, acid chlorides, chloroformates, carbamoyl chlorides, ClC(S)OPh(F5), thiocarbonyldimidazole, isocyanates, and the like, and amine bases according to general procedures known to those skilled in the art.
- Sulfonylations may be carried out using sulfonyl chlorides or sulfonic anhydrides.
- Alkylations may be carried out using alkyl halides or trichloroacetimi dates.
- Suitable solvents for these reactions include benzene, toluene, CHCI3, CH2CICH2CI, and the like, or most preferably CH2CI2, and may be performed from temperatures of -40°C to 80°C.
- the hydroxyl group at C8 of compound 11a can be eliminated using Burgess reagent, Martin's sulfurane reagent or by treating compound lid with a base to generate a mixture of C6, C7- and C7, C8-olefin isomers.
- Suitable bases include, but are not limited to, Et3N, EtN(z ' Pr)2, NaOMe, KOtBu, and the like or most preferably DBU in solvents such as CH2CI2, CHCI3, toluene, benzene, MeOH, EtOH, pyridine and the like and at temperatures from 0°C to 110°C.
- the C8-hydroxyl group of compound 11a can also be eliminated by reduction via the intermediary compound lie wherein R is OPh, OPh(F5), Set, and the like, or most preferably N-1-imidazolyl.
- Intermediary compound lie is treated with i) a radical initiator such as oxygen/Et3B,
- AIBN (2,2'-azobisisobutyronitrile), benzoyl peroxide and the like, and ii) a hydride source, including, but not limited to, Et3SiH, Me3SnH, Ph3SnH, Ph3AsH, nBu3SnCl/NaBH4, and the like, or most preferably «Bu3SnH in solvents including but not limited to CH2CI2, CHCI3, benzene, MeOH, EtOH, or most preferably toluene, and the like, at temperatures from
- Apicidin can be treated with mono- or disubstituted amines, a hydride source, and a proton source to generate compound llf.
- Suitable solvents include, but are not restricted to, benzene, toluene, EtOH, PrOH and the like, or more preferably,
- Suitable proton sources include, but are not limited to, TsOH, HC1, HCO2H,
- the intermediate imine may be reduced in situ as it is formed or after azeotropic removal of water using a Dean-Stark trap.
- Suitable reducing agents include, but are not limited to, LiAlH4, NaBH4, LiBEL)., H2/(10% Pd/C) and the like, or most preferably NaBH3CN.
- Oxime compound llg and hydrazone compound llh are prepared by treating apicidin with hydrazine in a solvent with a proton source.
- apicidin can be treated with mono- or disubstituted amines, and a proton source.
- Suitable solvents include, but are not restricted to, benzene, toluene, EtOH, iPrOH and the like, or more preferably, MeOH.
- Suitable proton sources include, but are not limited to, TsOH, HC1, HCO2H, PPTS and the like, or most preferably HOAc.
- olefination reactions may be performed in solvents including, but not limited to, DMF (N,N-dimethylformamide), MeOH, CH2CI2, toluene, Et2 ⁇ , MeC ⁇ , THF and the like and may be performed at from -78°C to 110°C.
- solvents including, but not limited to, DMF (N,N-dimethylformamide), MeOH, CH2CI2, toluene, Et2 ⁇ , MeC ⁇ , THF and the like and may be performed at from -78°C to 110°C.
- a solvent such as tBuOH, dimethoxyethane, THF, DMF, DMSO, or more preferably HMPA and a strong base such a tBuOK, nBuLi, or more preferably NaH at temperatures from -78°C to 50°C.
- nucleophiles include NaSMe, KSAc, HSPh Et3N, HSCH2CH2OH/EtN( Pr)2 and the like. These reactions proceed readily in polar solvents such as MeOH, EtOH, DMF, DMSO, HMPA, NMP and the like at temperatures from 0°C to 50°C.
- a Beckmann rearrangement to form compounds 12a and 12b can be induced by treatment of compound llg with an acylating agent, including but not limited to, POCI3, SOCI2, MeSO2Cl and the like or more preferably TsCl and an amine base at temperatures from 0°C to 50°C.
- Suitable amine bases include Et3N, EtN(iPr)2, lutidine, DBU (l,8-diazabicyclo[5.4.0]undec-7- ene) and the like, or most preferably pyridine.
- Pyridine also may serve as a solvent for this reaction or alternatively MeCN, benzene, toluene, dioxane and the like may be used.
- the C7-aldehyde compound 6 could be oxidized to the corresponding C7 methyl ester compound 13 by treating with suitable oxidants including NaOCl/HOAc/MeOH, tBuOCl/MeOH/pyridine, and the like, or most preferably PDC/DMF/MeOH under conditions known in the art.
- suitable oxidants including NaOCl/HOAc/MeOH, tBuOCl/MeOH/pyridine, and the like, or most preferably PDC/DMF/MeOH under conditions known in the art.
- the C7 methyl ester compound 13 can further serve as the starting material for additional derivatives.
- the C6-aldehyde compound 10 can be oxidized to its corresponding C6 methyl ester (not shown).
- methyl ester compounds 7 and 13 can be converted into a series of esters, amides and ketones.
- Rl4a > Rl4bL and Rl4b2 > is each independently an alkyl or aryl group, which optionally is substituted.
- Saponification could be accomplished by treating compound 7 with reagents including, but not limited to, NaOH, KOH, Me3SiOOK, LiOOH and the like, or more preferably LiOH.
- Solvents, or mixtures of solvents include MeOH, EtOH, tBuOH, DMF, DMSO, HMPA, Et2 ⁇ , THF, water and the like.
- the reaction proceeds at temperatures from 0°C to 100°C.
- Amide and ester formation may be accomplished by reacting the C8-carboxylic acid (compound 14a) thus prepared using standard ester- and amide-forming reagents known to those skilled in the art.
- the esterification reaction is carried out using at least one equivalent of an alcohol, HOR.
- Esterification reagents include, but are not restricted to, dicyclohexylcarbodiimide, l-(3-dimethylaminopropyl)-3- ethylcarbodiimide hydrochloride (EDC » HC1), diisopropylcarbodiimide, benzotriazol- l-yloxy-tris(dimethylamino)phosphonium hexafluorphosphate (BOP), bis(2-oxo-3- oxazolidinyl)phosphinic chloride (BOP-C1), benzotriazole-1-yl-oxy-tris-pyrrolidino- phosphonium hexafluorophosphate (PyBOP), chloro-tris-pyrrolidino-phosphonium hexafluorophosphate (PyClOP), bromo-tris
- the ester-forming reactions may be facilitated by the optional addition of N-hydroxybenzotriazole, N-hydroxy-7-aza-benzotriazole, 4- (N,N-dimethylamino)pyridine or 4-pyrrolidinopyridine.
- the ester-forming reaction is generally performed using at least one equivalent (although several equivalents may be employed) of amine bases such as triethylamine, diisopropylethylamine, pyridine and the like.
- the carboxyl group may be activated for ester bond formation via its corresponding acid chloride or mixed anhydride, using conditions known to those skilled in the art.
- the ester-forming reaction is carried out in an aprotic solvent such as, for example, methylene chloride, tetrahydrofuran, diethyl ether, dimethylformamide, N-methylpyrrolidine, and the like, at temperatures ranging from - 20°C to 60°C, and is complete in about 15 minutes to about 24 hours.
- an aprotic solvent such as, for example, methylene chloride, tetrahydrofuran, diethyl ether, dimethylformamide, N-methylpyrrolidine, and the like
- Amides (where Rl2 is NRi4 lRl4b2) are prepared as described for esters (vida supra) from the corresponding carboxylic acids using and an appropriate amine, HNRi4blRl4b2-
- the amide compound 14b (in which NRi4blRl4b2 is N(OMe)Me) can be treated with nucleophilic agents to yield the corresponding aldehyde (compound 14c) and ketones (compounds 14d and 14e).
- Suitable nucleophiles include, but are not limited to, hydride reagents, RLi or RMgX and the like as described above for the preparation of compounds 11a and lib.
- the aldehyde and ketone products 14c, 14b and 14e can be further reacted with hydride reagents, RLi or RMgX, to generate the corresponding alcohol adducts as described previously.
- aldehyde compounds 6, 10 and 14c serve as starting material for the preparation of a variety of derivatives.
- the side chain azide compound 15c can be reduced using conditions known to those skilled in the art including, but not restricted to, H2/10% Pd/C, HSAc/MeOH, SnCl2, Ph3P/H2 ⁇ and the like to form a side chain amine compound (not shown).
- the amine compound thus obtained can be acylated, alkylated or sulfonylated as described above.
- reductive amination of the aldehyde compounds 6, 10 and 14c with a suitable amine as described above will generate the amine compound 15d.
- the side chain of compounds 6, 10 or 14c can be extended by reacting the aldehyde with stabilized Wittig reagents, unstabilized Wittig reagents or Horner-Emmons reagents to form compound 16a.
- the side chain unsaturation of compound 16a can be reduced by catalytic hydrogenation using conditions known to those skilled in the art.
- Suitable catalysts include 5% Pd/C, 10% Pd/C, Pd(OH)2, Pt ⁇ 2, RhCl3, RuCl2(PPh3)3, and the like.
- the hydrogenation reactions may be performed in solvents or mixtures of solvents including CH2CI2, CHCI3, toluene, MeOH, EtOH, EtOAc, acetone, THF, Et2 ⁇ , dimethoxyethane, DMF, DMSO, and the like.
- the reductions may be run at from one to 10 atmospheres of hydrogen pressure and the reactions are complete in from 5min to 24h.
- the ester may be saponified and the carboxylic acid thus obtained may be converted into other esters or amides as described previously.
- the N-methoxy group of apicidin may be removed by hydrogenation as described previously and the liberated indole nitrogen compound thus generated may be N-alkylated, acylated or sulfonylated using known methods for acylation, sulfonylation and alkylation of indoles to generate apicidin derivative compound 17.
- Rl7 is an alkyl or aryl group, which optionally is substituted.
- acylation may be accomplished using reagents such as acid anhydrides, acid chlorides, chloroformates, carbamoyl chlorides, isocyanates and the like according to general procedures known to those skilled in the art.
- Sulfonylations may be carried out using sulfonyl chlorides or sulfonic anhydrides.
- Alkylations may be carried out using alkyl halides.
- Suitable bases for these acylation, sulfonylation and alkylation reactions include KH, nBuLi, tBuLi, LiN( Pr)2, NaN(SiMe3)2, KN(SiMe3)2 and the like or more preferably NaH.
- Suitable solvents, or mixtures of solvents for these reactions include benzene, toluene, CHCI3, CH2CICH2CI, CH2CI2, DMSO, HMPA, NMP and the like or most preferably DMF and may be performed from temperatures of -40°C to 80°C.
- the apicidin derivative compound 17 can be saponified to the corresponding carboxylic acid and converted into a series of amides using conditions described previously.
- the apicidin derivative compound 17 can be acylated, alkylated, phosphorylated or sulfonylated as described previously.
- this alcohol function may be converted into a leaving group such as a sulfonate or halide and displaced with appropriate sulfur, nitrogen or phosphorus nucleophiles as described previously
- apicidin's tryptophan may be allylically oxidized to generate beta-oxo apicidin analog compound 18 using conditions known to those skilled in the art. . (What is Rl8?)
- Rl8 is an alkyl or aryl group, which optionally is substituted.
- Suitable oxidants include but are not restricted to tBuOOH, Se ⁇ 2, Cr ⁇ 3, Na2CrO4, PCC, and the like, or more preferably DDQ (2,3-dichloro-5,6- dicyano-l,4-benzoquinone).
- Appropriate solvents, or mixtures of solvents include DMF, toluene, benzene, CH2CI2, CHCI3, HOAc, pyridine, THF, MeOH, EtOH, water, and the like, or more preferably MeCN. These reactions are performed at from -20°C to 50°C and are complete in from 5min to 24h.
- the stereochemistry of the beta-oxo-tryptophan attachment of compound 18 may be changed by treatment with bases such as pyridine, EtN( ⁇ Pr)2, NaH, KH, DBU, lutidine, or most preferably Et3N.
- bases such as pyridine, EtN( ⁇ Pr)2, NaH, KH, DBU, lutidine, or most preferably Et3N.
- the epimerization reaction proceeds at from 0°C to 50°C in solvents including CHCI3, CH2CICH2CI, MeOH, EtOH, DMF, DMSO, NMP, and the like, or most preferably CH2CI2.
- the nitrogen of the beta-oxo-tryptophan may be alkylated, acylated, sulfonylated or phosphorylated as described previously.
- the beta-oxo carbonyl of compound 18 may be selectively reduced using a hydride source under radical conditions.
- Suitable hydride sources include Me3SnH, «Bu3SnCl/NaBH4, Ph3SnH, Ph3AsH, and the like, or most preferably nBu3SnH, in the presence of radical initiators.
- Suitable radical initiators include, for example, benzoyl peroxide, Et3B/O2, and the like, or most preferably AIBN.
- Rl9a Rl9b> Rl9c, and Rl9d are each independently an alkyl or aryl group, which optionally is substituted.
- Suitable oxidants include, but are not restricted to, KMn ⁇ 4, KMnO4/NaIO4, NaIO4/RuO4, and the like, or most preferably Na >4/RuCl3.
- Suitable solvents, or mixtures of solvents include CHCI3, CH2CICH2CI, MeCN, MeOH, EtOH, tBuOH, and the like, or most preferably CH2CI2. The reaction proceeds at temperatures from 0°C to 50°C. This carboxylic acid may be converted into esters or amides as described previously. Alternatively, a methyl ester may be prepared first (eg.
- Reduction of the sidechain C8-ketone group of compound 19a to the corresponding alcohol proceeds as described previously.
- the Weinreb amide thus directly generated can then be reacted with hydride reagents, RLi, or RMgX as described previously to prepare the corresponding aldehyde or ketones (eg. 19a where Rl9a is H, alkyl or aryl group).
- the side chain C8-alcohol may be oxidized back to regenerate the C8-ketone as described previously.
- Rl9a is OH in compound 19a
- the carboxylic acid may be reduced using BH3 to form an alcohol compound 19c (where Rl9c is H).
- This alcohol may be acylated, sulfonylated or phosphorylated as described previously.
- R20 and R21 are each independently an alkyl or aryl group which optionally is substituted.
- Suitable oxidants include KMn ⁇ 4, NaIO4, Pb(OAc)4, and the like, or more preferably ozone.
- This reaction may be run in solvents such as CHCI3, CH2CICH2CI, and the like, or more preferably CH2CI2, at temperatures from -78°C to RT and the reaction is complete in from lmin to 2h.
- Treatment of compound 20 with a base induces Aldol cyclization to form a quinolone compound 21.
- Suitable bases for this reaction include Et3N, EtN( Pr)2, pyridine, DBU, NaOMe, NaOEt, NaHCO3 ? an d the like, or more preferably KOtBu.
- the Aldol cyclization may be performed in solvents, or mixtures of solvents including CH2CI2, CHCI3, MeOH, EtOH, DMF, THF, Et2 ⁇ , DMSO, water, and the like, or more preferably tBuOH.
- the reaction is complete in from lOmin to 12h at 0°C to RT.
- Substitution of N- substituted-N-desmethoxy-apicidin derivatives (Compound 17) for apicidin in Scheme XII leads to the formation of N-substituted quinolone derivatives.
- the quinolone compound 21 can be treated with sulfonylating agents as described previously to form compound 22 wherein R22 i a sulfonate moiety
- R22 and R23 are each independently an alkyl or aryl group, which optionally is substituted.
- R22 of compound 22 is OSO2CF3
- the triflate can be displaced with suitable nucleophiles, such as halogen, sulfur nucleophiles or nitrogen nucleophiles including, but not limited to, NaBr, NaCl, KI, NaN3, NaSMe, KSAc, pyridine and the like.
- suitable nucleophiles such as halogen, sulfur nucleophiles or nitrogen nucleophiles including, but not limited to, NaBr, NaCl, KI, NaN3, NaSMe, KSAc, pyridine and the like.
- Suitable solvents for the displacement reaction include, but are not limited to, CH2CI2, CHCI3, DMF, DMSO, HMPA, NMP, and the like.
- the reactions proceed at temperatures from 0°C to 80°C.
- the pyridinium group may be reduced using catalytic hydrogenation as described previously.
- apicidin derivative compound 21 may be reduced first.
- the thus formed quinolone carbonyl can then be reacted with nucleophiles such as hydride reagents, RLi or RMgX as described previously.
- nucleophiles such as hydride reagents, RLi or RMgX as described previously.
- the apicidin derivative compound 23 can be prepared by reoxidation of the C8-alcohol as described previously.
- apicidin may be brominated at the indole C2 position following removal of the N-methoxy group using conditions known to those skilled in the art to form compound 24 where R24 is Br.
- Suitable brominating agents include, but are not limited to, Br2,
- the bromination reaction can be facilitated by a radical initiator such as benzoyl peroxide, Et3B/O2 or AIBN.
- the 2-bromo-indole thus obtained can be further reacted with a palladium catalyst, a base and ArX to induce an aryl coupling reaction.
- Suitable palladium catalysts include, but are not limited to, Pd(OAc)2, Pd(OAc)/PPh3, PdCl2(PPh3)2, Pd(dba)2/PPh3, and the like, or most preferably Pd(PPh3)4.
- Suitable bases for this reaction include, but are not limited to, KOtBu, CsCO3, or most preferably NaHCO3.
- Suitable solvents, or mixtures of solvent for this coupling reaction include toluene, DMF, MeCN, NMP, DMSO, H2O, EtOH, or most preferably dioxane/water.
- Suitable ArX groups include, but are not limited to, PhB(OH)2, 2-napthylboronic acid, (4-Me)PhB(OH)2, (4-F)PhOTf, and the like. The reactions are complete in from 30min to 48h at temperatures from RT to 110°C.
- RT Room temperature
- Example 1 was prepared by the following procedure. At room temperature, 27mg of Me3S(O)I was added to a mixture of i) 5.6mg of 60% NaH and ii) 0.35mL HMPA. The resulting solution was allowed to stand for 5min. Then, a mixture of 12mg apicidin in 96 ⁇ L DMF was added to form a reacting mixture. After
- Example 1 was thus obtained without requiring further purification and was characterized by H NMR and MS [m/z: 638 (M +1)].
- Example 2 was prepared by the following procedure. At room temperature, 60mg HCl » H2NOH and 181 ⁇ L Et3N was added to 20mg apicidin in lOmL CH2C12- The resulting solution was aged for 12h. The volatiles were then removed under reduced pressure.
- Example 2 was obtained following preparative RP- HPLC (reversed phase high performance liquid chromatography), without workup, using a gradient elution characterized by 1:3 MeCN:H2 ⁇ to 100% MeCN, with a
- Examples 3a-3m were prepared following the general procedure described in Scheme HI for compound 1 If, 1 lg, and 1 lh, and for Ex.2. Examples 3a- 3m are described by the following chemical formula and were characterized by NMR and mass spectroscopy:
- Examples 4a and 4b were prepared by the following procedure. At 0°C, 4.5mg of p-toluenesulfonyl chloride was added to 3mg of Example 2 (the C8- ketoxime of apicidin) in 0.5mL pyridine to form a solution. The solution was maintained at 0°C for lOmin, then warmed to RT and aged for 50min. Then lmL each of saturated brine and saturated NaHCO3(aq) were added. Next, the solution was extracted with EtOAc and dried with Na2SO4.
- Example 5 was prepared by the following procedure. At room temperature (RT), 114mg of Ph3PCH3 + Br " was added to i) 16.8mg of a 60% dispersion of NaH in oil, ii) 2mL DMF, and iii) 0.2mL HMPA to form a mixture.
- Example 6a was prepared by the following procedure. At 0°C, 0.12mL of l.OM (4-Cl)PhMgBr in Et2 ⁇ was dropwise added to 15mg apicidin in a mixture of
- Examples 6b, 6c, and 6d are described by the chemical structure shown below. The specific substituents are tabulated in Table 2. Examples 6b, 6c, and 6d were prepared following the general procedure described in Scheme HI for compound lib under conditions similar to those described above for Ex. 6a
- Example 8 was prepared following the general procedure of Example 7 but using N-desmethoxy-apicidin as the starting material.
- Example 8 was characterized by H NMR and MS [m/z: 596 (M + +l)].
- Example 9 was prepared by the following process. At room temperature, 57mg of thiocarbonylimidazole was added to 40mg of cyclo(N-0- methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-8-hydroxy-decanoyl) in 1.6mL CH2O2. The resulting solution was heated to 75°C for 2 hours. Next, lmg of DMAP (4- dimethylaminopyridine) was added and the solution aged for lh at 75°C and 48h at RT. The solvent then was removed under reduced pressure.
- DMAP 4- dimethylaminopyridine
- Example 9 was characterized by H NMR and MS [m/z: 610 (M + +l)].
- Example 10 was made by adding lOmg DMAP to lOOmg cyclo N-0- methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-8-hydroxy-decanoyl) in 2mL pyridine at RT. Next, 94mg tosic anhydride was added. After 3d at RT, the solution was poured into saturated NaHCO3, extracted with CH2CI2 and dried with Na2SO4. Pure Example
- Example 10 was obtained following flash chromatography on silica gel using gradient elution (1:1:98 then 1:2:97 then 1:3:96 NH4OH:MeOH:CHCl3 as eluant).
- Example 11 The procedure to form Example 11 was as follows. At room temperature, 50mg NaBH4 was added to lOOmg apicidin in lOmL 1:1 THF:MeOH. After 30min at RT, the solution was poured into brine, extracted with CH2CI2 and dried with Na2SO4. To the residue thus obtained was added 2mL pyridine, followed by addition of lOmg DMAP and 10 drops of Ac2 ⁇ . After another 15min at RT, the solution was heated to 80°C for lOmin. without noting any reaction. An additional 5 drops of fresh Ac2 ⁇ (from an unopened bottle) were added and the solution stirred at
- Example 13 0.16mL (Me3Si)2NH and 235mg ZnCl2 was added to lOOmg apicidin in 5mL EtOAc at RT. The solution was heated to 55°C for 12 hours. The solution was then cooled to 0°C and 12mg NaBE ). was added. After lh, the solution was warmed to RT and aged an additional 2h. The solution was poured into 1:1 brine:saturated NaHCO3, extracted with CH2CI2 and dried with Na2SO4. Pure Example 13 product was obtained following preparative RP-HPLC using gradient elution (3:7 to 6:4 MeCN:H2 ⁇ ) and was characterized by H NMR and
- Example 15 was made by first adding to 60mg apicidin in 0.5mL
- Example 16 was prepared similarly to Example 15. At room temperature, to 60mg apicidin in 0.5mL MeOH was added i) 2mL pyridine, ii) 0.5mL propylamine, iii) lmL glacial HOAc (pH ⁇ 4.5), and iv) powdered 4A sieves. The solution was cooled to 0°C and 60mg NaCNBH3 was added. After 2h, the solution was warmed to RT and aged for 12h. The solution was filtered through Celite using 1:1 CH2 ⁇ 2:MeOH as eluant, reduced in volume in vacuo, poured into saturated NaHCO3, extracted with CH2CI2 and dried with Na2SO4.
- Example 16 was obtained following PTLC on silica gel (1 x 1500 ⁇ m plate) using 2:18:80 NH4OH:MeOH:CHCl3 as eluant.
- the pure Example 16 product thus obtained was characterized by H NMR and MS [m/z: 667 (M + +l)].
- Example 17 32mg KSAc was added to l ⁇ .lmg of the Example 10 C8-tosylate compound in 3mL 95% EtOH. The solution was heated to 70°C for 3 hours. The solution was then cooled to RT and saturated NH4Cl(aq) was added. Next, the solution was extracted with EtOAc and dried with Na2SO4. The solution then was filtered, evaporated to dryness. PTLC on silica gel (1 x lOOO ⁇ m plate) using 3:7 acetone:hexanes as eluant yielded 3.4mg of pure Example 17 product that was characterized by H NMR.
- Example 18 3.4mg of the Example 17 C8-thioacetate compound was placed at RT in 0.2mL NaOMe 2M solution in MeOH) and aged for 3h. The solution was poured into saturated NH4 ⁇ (aq), extracted with CH2CI2, and dried with Na2SO4. The solution was filtered, concentrated to dryness, and pure Example 18 was obtained following RP-HPLC. Example 18 thus obtained was characterized by H NMR.
- Examples 19a and 19b were prepared by the following procedure. 50mg apicidin was heated in 5mL THF at 50°C until the resulting solution became homogenous. The solution was then cooled to -78°C and immediately 800 ⁇ L 0.5M potassium hexamethyldisilazane in toluene was added. After
- Example 19a and Example 19b were used with no further purification in the next reaction.
- the crude yield was 74mg (145%).
- Example 19a cyc/o(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-8-trimethylsiloxy-7- ene- decanoyl), and Example 19b, cycZ ⁇ (N-O-Methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino- 8-trimethylsiloxy-8-ene-decanoyl), was placed in 5mL CH2CI2 at RT to which was added 200mg solid NaHCO3. To this solution was added 20mg 85% MCPBA.
- Example 20a cycZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-8-oxo-7- trimethylsiloxy-decanoyl)
- Example 20b cycZ ⁇ (N-O-Methyl-L-T ⁇ -L-Ile-D-Pip- L-2-amino-8-oxo-9-trimethylsiloxy-decanoyl) following flash chromatography on silica gel using 4:1 hexanes: acetone as eluant.
- Example 21a and Example 21b were prepared by the following procedure. To 43mg of a 1:1 mixture of Example 20a, cycZ ⁇ (N-O-methyl-L-T ⁇ -L- Ile-D-Pip-L-2-amino-8-oxo-7-trimethylsiloxy-decanoyl), and Example 20b, cyclo(N- O-Methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-8-oxo-9-trimethylsiloxy-decanoyl), in 4mL THF at RT was added 120 ⁇ L 1M nBu4NF in THF.
- Example 23a was prepared and characterized by H NMR.
- Examples 24a and 24b were prepared by adding lOmL pyridine to lOmg -1:1 mixture of Examples 21a and 21b in 3mL MeOH at 0 °C, followed by the addition of lOmg Pb(OAc)4. After lOmin, the solution was quenched with 2mL Na2S2 ⁇ 3, diluted with about 2mL brine, extracted with CH2CI2 and dried with Na2SO4. Following preparative TLC on silica gel (500 ⁇ m plate) using 1:2 acetone: hexanes as eluant, separated pure products were obtained.
- Examples 25a-25d were prepared by following the general procedure of Example 24b. Starting with Examples 21a and 21b, and using an appropriate alcohol as solvent, the following derivatives were prepared and analyzed by NMR and mass spectroscopy:
- Example 26 was prepared by the following procedure. To 41mg cycZo(N-O-methyl-L-T ⁇ -L-Ue-D-Pip-L-2-amino-7-carbomethoxy-heptanoyl) in 4mL 3:1:1 THF:MeOH:H2 ⁇ at 0°C was added lOO ⁇ L IM LiOH. The solution was stirred for lh and then additional 300 ⁇ L IM LiOH was added. After 12h, 33mg pure
- Example 26 product was obtained following preparative RP-HPLC without workup using gradient elution (column equilibrated in 5:95 MeCN:H2 ⁇ , using 25:75 MeCN:H2 ⁇ for 40min followed by a 20min ramp to 100% MeCN, flow rate lOm min).
- Example 26 was characterized by H NMR and MS [m z: 629.2
- Example 27 was prepared by the following procedure. To 15mg cycZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-carboxy-heptanoyl), lithium salt in 3mL DMF at RT was added 5.4mg H2NOSi(Me)2tBu and 7mg EDC-HC1. After 2h at RT, 15mg additional H2NOSi(Me)2tBu (15mg) and 14mg EDC-HC1 were added and the solution allowed to stir overnight. The reaction was quenched by the addition of 5 drops glacial HOAc and lmL MeOH. The solution was poured into brine, extracted with CH2CI2, and dried with Na2SO4.
- the crude product was chromatographed on silica gel using gradient elution (1:3:96 NH4OH:MeOH:CHCl3 to 1:4:95 NH4OH:MeOH:CHCl3, to 1:9:90 NH4OH:MeOH:CHCl3).
- the product was dissolved in 2mL CHCI3 and 2mL 10% aq. HOAc. After 5min, the aqueous layer was decanted and the washing repeated twice more to yield 5.5mg pure Example 27 product.
- the pure Example 27 stained positive (pu ⁇ le-orange) for a hydroxamic acid using Fe ⁇ CI3 stain.
- the product was characterized by H NMR and MS [m/z: 627.3 (M + +l)].
- TLC: Rf 0.26 (then 1:9:90 NH4OH:MeOH:CHCl3).
- Example 28 was prepared by the following procedure. To 30mg cycZo(N-O-methyl-L-T ⁇ -L- ⁇ e-D-Pip-L-2-amino-7-carboxy-heptanoyl) lithium salt in lmL DMF at RT was added 47mg HCl*HN(OMe)Me, 2mg DMAP, 7mg HOBT (1- hydroxybenzotriazole hydrate) and 90 ⁇ L DIEA (Et2Nz ' Pr) followed by 12mg EDCI
- Example 29 was prepared by the following procedure. To 150mg c cZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-carboxy-heptanoyl) in 14mL CH2CI2 at 0°C was added 78mg HCl-H2NOCH2Ph, 0.13mL DIEA, 33mg HOBT,
- Example 30 was prepared by the following procedure. To 130mg cycZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-(N-benzyloxy-carboxamido)- heptanoyl) in 5mL MeOH at RT was added 5% Pd/C and an H2 atmosphere (balloon pressure) was established. After 12h, lOmg Pd(OH)2 was added and the reaction continued for an additional 2h. The catalyst was removed by filtration through Celite using MeOH as eluant and the solution concentrated under reduced pressure. Pure Example 30 product was obtained following RP-HPLC purification using gradient elution (5:95 MeCN:H2 ⁇ for 5min then 55min ramp to 50:50 MeCN:H2 ⁇ ). The pure
- Example 30 was characterized by l H NMR and MS [m/z: 597.5 (M + +l)].
- TLC: Rf 0.11 (1:9:90 NH4OH:MeOH:CHCl3).
- HPLC: tR 10.65min (2min ramp from 5:95 MeCN:H2 ⁇ to 1:1 MeCN:H2 ⁇ , l.OmL/min, ZorbaxTM RX-8).
- Example 32 was prepared by the following procedure. To 7mg cyc/o(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-(N-methoxy-N-methyl- carboxamido)-heptanoyl) in lmL THF at 0°C was added 55 ⁇ L IM MeMgBr in Et2 ⁇ . After lOmin, an additional 55 ⁇ L IM MeMgBr in Et2 ⁇ was added. The solution was poured into saturated NH4CI, extracted with CH2CI2 and dried with Na2SO4.
- Example 32 product was obtained following preparative TLC on silica gel (1 x 500 ⁇ m plate) using 4:6 acetone: hexanes as eluant.
- the pure Example 32 was characterized by ! H NMR and MS [m/z: 610.3 (M + +l)].
- TLC: Rf 0.22 (1:2 acetone: hexanes).
- HPLC: tR 4.51 min (1:1 MeCN:H2 ⁇ , 1.5mL/min, ZorbaxTM RX-8).
- Example 34 was prepared by the following procedure. To 25mg cycZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-oxo-heptanoyl), 1 lmg anhydrous LiCl, and 2 lmL (MeO)2P(O)CH2CO2Me in 2.5mL MeCN at RT, was added 42mL DIEA. After 2h the solution was poured into saturated NaHCO3, extracted with
- Example 34 product was obtained following flash chromatography on silica gel using 1:2 acetone: hexanes as eluant. The pure Example 34 was characterized by *H NMR and MS [m/z: 638.2 (M + +l)].
- TLC: Rf 0.38 (1:2 acetone:hexanes).
- HPLC: tR 5.09min, (1:1 MeCN:H2 ⁇ , 1.5mL/min, ZorbaxTM RX-8).
- Example 35 was prepared by the following procedure. To 35mg cyc/o(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7,8-dehydro-8-carbomethoxy- octanoyl) in 4mL 1:1 THF:MeOH was added 20mg Pd(OH)2 and an H2 atmosphere
- Example 36 was prepared by the following procedure. To 10.6mg cycZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-8-carbomethoxy-octanoyl) in lmL 3:1:1 THF:MeOH:H2 ⁇ at 0°C was added 15mL IM LiOH. The solution was stirred for lh at 0°C, 6h at RT, 3 days at 4°C and then an additional 30mL IM LiOH was added.
- Example 37 was prepared by the following procedure. To 25mg cyc/o(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-oxo-heptanoyl) in 1.25mL DMF at RT was added 0.25mL MeOH followed by 67.5mg PDC. The solution was stirred for 3.5h and then filtered through 1" silica gel, with 0.5" Celite on top of it, using MeOH as eluant. The solvents were removed under reduced pressure. Pure 9mg Example 37 product was obtained following preparative TLC on silica gel (2 x lOOO ⁇ m plates) using 5:95 MeOH:CHCl3 as eluant. The pure Example 37 was characterized by H
- Example 38 was prepared by following the general procedure of Example 15, and Scheme IH, utilizing methyl glycinate in place of ethylamine, and was characterized by H NMR and MS [m z: 655.0 (M + +l)].
- Example 39 was prepared by following the general procedure of Example 36 and starting with the methyl ester of Example 38, and was characterized by *H NMR and MS [m z: 641.4 (M + +l)].
- Example 40 was prepared by following the general procedure of Example 7, utilizing Example 32 as the starting material, and was characterized by H NMR and MS [m/z: 598.3 (M + +l)].
- Example 41 was prepared by following the general procedure of Example 7 to convert the C7-aldehyde of Example 23 and was characterized by H NMR and MS [m/z: 584.2 (M + +l)].
- Example 42 was prepared by the following two methods.
- Example 42 Following the general procedure of Example 7, the C6-aldehyde of Example 58a was converted into Example 42 by adding 2.1mg NaBH4 to 64mg cycZ ⁇ (N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-6-oxo-hexanoyl) in lmL 1:1 THF:EtOH at 0°C. After lh, the resulting solution was poured into saturated NH4CI, extracted exhaustively with CH2CI2 and 3:7 ZPrOH:CHCl3 (lx). The organic layer was dried with Na2SO4.
- Example 42 was obtained following PTLC on silica gel (1 x 500 ⁇ m plate) using 1:1 acetone:hexanes as eluant.
- Example 42 was characterized by ! H NMR and MS [m/z: 570 (M + +l)].
- Example 55a and 55b 7.3mg of a -1:1 mixture of 6,7- and 9,10-enones of apicidin, Example 55a and 55b, was placed in lmL CH2CI2 at -78°C. Ozone was bubbled through the solution until a blue color persisted. A vigorous stream of nitrogen was then used to remove the excess ozone. To this solution was added 3.6mg NaBH4 in 120 ⁇ L 1:1 EtOH:H2 ⁇ , the cooling bath was removed and the solution was aged overnight. The solution was poured into saturated NH4Cl(aq), extracted with CH2CI2 and dried with Na2SO4. Pure Example 42 was obtained following PTLC purification on silica gel (1 x 500 ⁇ m plate) using 1:1 acetone:hexanes as eluant. EXAMPLE 43
- Example 43 was prepared by the following procedure. To 32mg Example 41, cycZ ⁇ (N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-hydroxy-heptanoyl), in 2.5mL CH2CI2 at 0°C, was added 27 ⁇ L DIEA, a catalytic amount of DMAP, and
- Example 44 was prepared from Example 42 cycZo(N-O-methyl-L-T ⁇ -
- Example 45 was prepared by the following procedure. To 9 ⁇ L (MeO)2P(O)H in 350 ⁇ L THF was added 2.5mg 95% NaH at RT via syringe and the solution heated to reflux for 20min. The solution was then cooled to RT and 25mg cycZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-(/? ra-toluenesulfonyl)-heptanoyl) was added as a solution in 350 ⁇ L THF, heated to reflux for 2h, cooled to RT and stirred for 12h. The solution was poured into saturated NaHCO3, extracted CH2CI2 and dried with Na2SO4. Pure 4.1mg Example 45 product was obtained following PTLC (1 x lOOO ⁇ m plate) on silica gel using 1:3:96 NH4OH:MeOH:CHCl3 as eluant.
- Example 48 was prepared by starting with Example 22b and following the general procedure described for Example 7.
- Example 22b' s C8 ketone group was converted to a hydroxyl to form Example 48, which was characterized by H NMR.
- Example 49 was prepared by the following procedure. A solution of 63 ⁇ L dibenzyl phosphonate in lmL THF was added via syringe to 7mg 95% NaH and the solution heated to reflux for 20min. The mixture was cooled to RT and 70mg cycZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-(p ⁇ ra-toluenesulfonyl)-octanoyl) was added as a solution in lmL THF. The resultant white, heterogeneous solution was heated to reflux for 2h followed by 12h at RT. The solution was added to water, extracted with CH2CI2 and dried with Na2SO4.
- Example 49 was obtained following PTLC on silica gel (1 x 1500 ⁇ m plate) using 1:3:96 NH4OH:MeOH:CHCl3 as eluant. The product was characterized by H NMR and MS [m/z: 828 (M + +l)].
- Example 50 was prepared by the following procedure. To llmg c cZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-dibenzylphosphono-octanoyl), in 2mL /PrOH containing 44 ⁇ L H2O and 1.3mg KHCO3 at RT was added lmg 10% Pd/C. An atmosphere of H2 was established (balloon pressure). After 12h, the catalyst was removed by filtration through Celite using 1:1 MeOH:H2 ⁇ as eluant. The solution was concentrated in vacuo and the residue was washed with CHCI3 followed by EtOAc. The remaining glassy material was lyophilized from water to yyiieelldd 33mmgg product. The product was characterized by H NMR and MS [m/z: 738 (M + +l)].
- Example 51 was prepared by the following procedure. To 2mg c ⁇ cZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-dibenzylphosphono-octanoyl) in 0.35mL iPrOH was added 8 ⁇ L water, 0.25mg KHCO3 and 0.5mg 10% Pd/C and a balloon atmosphere of hydrogen was established. After 7h at RT, the catalyst was removed via filtration through Celite and washed with water. 3mg of pure Example 51 product was characterized by H NMR and MS [m/z: 648 (M + +l)].
- Examples 52a and 52b were prepared by the following procedure. To 3mg -1:1 c cZo(N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-7-hydroxy-8-oxo- decanoyl) and cycZ ⁇ (N-O-methyl-L-T ⁇ -L-Ile-D-Pip-L-2-amino-9- hydroxy -8-oxo- decanoyl) 3mg) in 0.25mL CH2CI2 at -78°C was added powdered, activated 4A sieves followed by 1.5 ⁇ L Et2NSF3.
- Example 52a and 52b were obtained following PTLC on silica gel (1 x 500 ⁇ m plate) using 1:3:96 NH4OH:MeOH:CHCl3 as eluant. 2.5mg of the mixture were characterized by H NMR and MS [m/z: 641 (M + +l)].
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EP00947507A EP1204411A1 (en) | 1999-07-23 | 2000-07-19 | Apicidin-derived cyclic tetrapeptides |
JP2001511926A JP2003505417A (en) | 1999-07-23 | 2000-07-19 | Apicidin-derived cyclic tetrapeptides |
CA002378849A CA2378849A1 (en) | 1999-07-23 | 2000-07-19 | Apicidin-derived cyclic tetrapeptides |
AU61096/00A AU6109600A (en) | 1999-07-23 | 2000-07-19 | Apicidin-derived cyclic tetrapeptides |
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US14532999P | 1999-07-23 | 1999-07-23 | |
US60/145,329 | 1999-07-23 |
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JP (1) | JP2003505417A (en) |
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Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003057722A2 (en) * | 2001-12-28 | 2003-07-17 | Fujisawa Pharmaceutical Co., Ltd. | Cyclic tetrapeptide compound and use thereof |
WO2003070754A1 (en) * | 2002-02-20 | 2003-08-28 | Minoru Yoshida | Histone deacetylase inhibitors and process for producing the same |
WO2004113366A1 (en) * | 2003-06-20 | 2004-12-29 | Riken | Histone deacetylase inhibitor and process for producing the same |
US7566765B2 (en) | 2000-03-06 | 2009-07-28 | Rigel Pharmaceuticals, Inc. | Heterocyclic compounds containing a nine-membered carbon-nitrogen ring |
US7732475B2 (en) | 2005-07-14 | 2010-06-08 | Takeda San Diego, Inc. | Histone deacetylase inhibitors |
US20120034593A1 (en) * | 2009-04-06 | 2012-02-09 | Centre National De La Recherche Scientifique | Cyclic peptides with an anti-parasitic activity |
US8158587B2 (en) | 2006-09-05 | 2012-04-17 | Kyushu Institute Of Technology | Compound having histone deacetylase-inhibiting activity, and pharmaceutical composition comprising the compound as an active ingredient |
EP3461480A1 (en) | 2017-09-27 | 2019-04-03 | Onxeo | Combination of a dna damage response cell cycle checkpoint inhibitors and belinostat for treating cancer |
EP3461488A1 (en) | 2017-09-27 | 2019-04-03 | Onxeo | Combination of a dbait molecule and a hdac inhibitor for treating cancer |
WO2021148581A1 (en) | 2020-01-22 | 2021-07-29 | Onxeo | Novel dbait molecule and its use |
WO2022150167A3 (en) * | 2020-12-17 | 2022-09-29 | University Of Florida Research Foundation, Incorporated | Novel macrocyclic opioid peptides |
WO2023041805A1 (en) | 2021-09-20 | 2023-03-23 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for improving the efficacy of hdac inhibitor therapy and predicting the response to treatment with hdac inhibitor |
WO2023194441A1 (en) | 2022-04-05 | 2023-10-12 | Istituto Nazionale Tumori Irccs - Fondazione G. Pascale | Combination of hdac inhibitors and statins for use in the treatment of pancreatic cancer |
Families Citing this family (1)
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CA2978730A1 (en) * | 2015-03-06 | 2016-09-15 | Colorado State University Research Foundation | Synthesis and utility of new capgroup largazole analogs |
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US5620953A (en) * | 1994-07-27 | 1997-04-15 | Merck & Co., Inc. | Antiprotozoal cyclic tetrapeptides |
US5922837A (en) * | 1995-09-20 | 1999-07-13 | Merck & Co., Inc. | Antiprotozoal cyclic tetrapeptides |
EP1010705A1 (en) * | 1997-09-02 | 2000-06-21 | Japan Energy Corporation | Novel cyclic tetrapeptide derivatives and medicinal use thereof |
-
2000
- 2000-07-19 AU AU61096/00A patent/AU6109600A/en not_active Abandoned
- 2000-07-19 EP EP00947507A patent/EP1204411A1/en not_active Withdrawn
- 2000-07-19 WO PCT/US2000/019627 patent/WO2001007042A1/en active Search and Examination
- 2000-07-19 JP JP2001511926A patent/JP2003505417A/en not_active Withdrawn
- 2000-07-19 CA CA002378849A patent/CA2378849A1/en not_active Abandoned
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US5620953A (en) * | 1994-07-27 | 1997-04-15 | Merck & Co., Inc. | Antiprotozoal cyclic tetrapeptides |
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EP1010705A1 (en) * | 1997-09-02 | 2000-06-21 | Japan Energy Corporation | Novel cyclic tetrapeptide derivatives and medicinal use thereof |
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US7566765B2 (en) | 2000-03-06 | 2009-07-28 | Rigel Pharmaceuticals, Inc. | Heterocyclic compounds containing a nine-membered carbon-nitrogen ring |
WO2003057722A3 (en) * | 2001-12-28 | 2004-04-22 | Fujisawa Pharmaceutical Co | Cyclic tetrapeptide compound and use thereof |
WO2003057722A2 (en) * | 2001-12-28 | 2003-07-17 | Fujisawa Pharmaceutical Co., Ltd. | Cyclic tetrapeptide compound and use thereof |
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US7488712B2 (en) | 2002-02-20 | 2009-02-10 | Kyushu Institute Of Technology | Histone deacetylase inhibitors and methods for producing the same |
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WO2004113366A1 (en) * | 2003-06-20 | 2004-12-29 | Riken | Histone deacetylase inhibitor and process for producing the same |
US7662778B2 (en) | 2003-06-20 | 2010-02-16 | Riken | Histone deacetylase inhibitor and process for producing the same |
US7732475B2 (en) | 2005-07-14 | 2010-06-08 | Takeda San Diego, Inc. | Histone deacetylase inhibitors |
US7741494B2 (en) | 2005-07-14 | 2010-06-22 | Takeda San Diego, Inc. | Histone deacetylase inhibitors |
US8158587B2 (en) | 2006-09-05 | 2012-04-17 | Kyushu Institute Of Technology | Compound having histone deacetylase-inhibiting activity, and pharmaceutical composition comprising the compound as an active ingredient |
US20120034593A1 (en) * | 2009-04-06 | 2012-02-09 | Centre National De La Recherche Scientifique | Cyclic peptides with an anti-parasitic activity |
US9133237B2 (en) * | 2009-04-06 | 2015-09-15 | Universite Joseph Fourier (Grenoble 1) | Cyclic peptides with an anti-parasitic activity |
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EP3461488A1 (en) | 2017-09-27 | 2019-04-03 | Onxeo | Combination of a dbait molecule and a hdac inhibitor for treating cancer |
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WO2022150167A3 (en) * | 2020-12-17 | 2022-09-29 | University Of Florida Research Foundation, Incorporated | Novel macrocyclic opioid peptides |
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EP1204411A1 (en) | 2002-05-15 |
CA2378849A1 (en) | 2001-02-01 |
JP2003505417A (en) | 2003-02-12 |
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