WO2001004360A3 - Retroviral recombination assays and uses thereof - Google Patents

Retroviral recombination assays and uses thereof Download PDF

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Publication number
WO2001004360A3
WO2001004360A3 PCT/US2000/018597 US0018597W WO0104360A3 WO 2001004360 A3 WO2001004360 A3 WO 2001004360A3 US 0018597 W US0018597 W US 0018597W WO 0104360 A3 WO0104360 A3 WO 0104360A3
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WO
WIPO (PCT)
Prior art keywords
retroviral
transfer
retroviral recombination
recombination assays
assays
Prior art date
Application number
PCT/US2000/018597
Other languages
French (fr)
Other versions
WO2001004360A2 (en
WO2001004360A9 (en
Inventor
John C Kappes
Xiaoyun Wu
John Wakeield
Original Assignee
Uab Research Foundation
Tranzyme Inc
John C Kappes
Xiaoyun Wu
John Wakeield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uab Research Foundation, Tranzyme Inc, John C Kappes, Xiaoyun Wu, John Wakeield filed Critical Uab Research Foundation
Priority to EP00947102A priority Critical patent/EP1226282A2/en
Priority to CA002379207A priority patent/CA2379207A1/en
Priority to AU60766/00A priority patent/AU6076600A/en
Publication of WO2001004360A2 publication Critical patent/WO2001004360A2/en
Publication of WO2001004360A3 publication Critical patent/WO2001004360A3/en
Publication of WO2001004360A9 publication Critical patent/WO2001004360A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
    • C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/48—Vector systems having a special element relevant for transcription regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods, systems, and cells are described that can be used to sensitively monitor for retroviral recombination. Using the invention, recombinant proviruses can be detected to provide an evaluation of the risk of creating a replication competent retrovirus (RCR) in vivo. The invention can further be used to improve on existing procedures in the art, including Tat transfer, Gag transfer, and marker rescue and mobilization assays.
PCT/US2000/018597 1999-07-09 2000-07-06 Retroviral recombination assays and uses thereof WO2001004360A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00947102A EP1226282A2 (en) 1999-07-09 2000-07-06 Retroviral recombination assays and uses thereof
CA002379207A CA2379207A1 (en) 1999-07-09 2000-07-06 Retroviral recombination assays and uses thereof
AU60766/00A AU6076600A (en) 1999-07-09 2000-07-06 Retroviral recombination assays and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US14301599P 1999-07-09 1999-07-09
US60/143,015 1999-07-09
US16462699P 1999-11-10 1999-11-10
US60/164,626 1999-11-10

Publications (3)

Publication Number Publication Date
WO2001004360A2 WO2001004360A2 (en) 2001-01-18
WO2001004360A3 true WO2001004360A3 (en) 2002-05-30
WO2001004360A9 WO2001004360A9 (en) 2002-09-12

Family

ID=26840604

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/018597 WO2001004360A2 (en) 1999-07-09 2000-07-06 Retroviral recombination assays and uses thereof

Country Status (4)

Country Link
EP (1) EP1226282A2 (en)
AU (1) AU6076600A (en)
CA (1) CA2379207A1 (en)
WO (1) WO2001004360A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6656706B2 (en) * 1999-12-23 2003-12-02 The United States Of America As Represented By The Department Of Health And Human Services Molecular clones with mutated HIV gag/pol, SIV gag and SIV env genes
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
US20040248083A1 (en) * 2001-05-17 2004-12-09 Mikkelsen Jacob Giehm Vectors for gene therapy
WO2012109500A2 (en) 2011-02-09 2012-08-16 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
CN105555972B (en) * 2013-07-25 2020-07-31 伯乐生命医学产品有限公司 Genetic assay
WO2018023094A1 (en) 2016-07-29 2018-02-01 Juno Therapeutics, Inc. Methods for assessing the presence or absence of replication competent virus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0463570A1 (en) * 1990-06-22 1992-01-02 R.O.B.I.T. RESEARCH AND DEVELOPMENT COMPANY LTD., c/o EFRATI, GALILI & CO. Detection of HIV infection by bioluminescence
WO1997008330A1 (en) * 1995-08-23 1997-03-06 Cancer Research Campaign Technology Limited Expression systems

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0463570A1 (en) * 1990-06-22 1992-01-02 R.O.B.I.T. RESEARCH AND DEVELOPMENT COMPANY LTD., c/o EFRATI, GALILI & CO. Detection of HIV infection by bioluminescence
WO1997008330A1 (en) * 1995-08-23 1997-03-06 Cancer Research Campaign Technology Limited Expression systems

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHANG L-J ET AL: "Efficacy and safety analyses of a recombinant human immunodeficiency virus type 1 derived vector system.", GENE THERAPY, vol. 6, no. 5, May 1999 (1999-05-01), pages 715 - 728, XP000872478 *
KAFRI T ET AL: "A packaging cell line for lentivirus vectors.", JOURNAL OF VIROLOGY, vol. 73, no. 1, January 1999 (1999-01-01), pages 576 - 584, XP002921432 *
NALDINI L ET AL: "In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector.", SCIENCE, vol. 272, no. 5259, 12 April 1996 (1996-04-12), pages 263 - 267, XP000583652 *

Also Published As

Publication number Publication date
CA2379207A1 (en) 2001-01-18
AU6076600A (en) 2001-01-30
WO2001004360A2 (en) 2001-01-18
EP1226282A2 (en) 2002-07-31
WO2001004360A9 (en) 2002-09-12

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