WO2001002583A3 - Orynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins - Google Patents

Orynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins Download PDF

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Publication number
WO2001002583A3
WO2001002583A3 PCT/IB2000/000973 IB0000973W WO0102583A3 WO 2001002583 A3 WO2001002583 A3 WO 2001002583A3 IB 0000973 W IB0000973 W IB 0000973W WO 0102583 A3 WO0102583 A3 WO 0102583A3
Authority
WO
WIPO (PCT)
Prior art keywords
pts
nucleic acid
acid molecules
proteins
genes encoding
Prior art date
Application number
PCT/IB2000/000973
Other languages
French (fr)
Other versions
WO2001002583A2 (en
Inventor
Markus Pompejus
Burkhard Kroeger
Hartwig Schroeder
Oskar Zelder
Gregor Haberhauer
Original Assignee
Basf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE1246922T priority Critical patent/DE1246922T1/en
Priority to HU0203191A priority patent/HUP0203191A3/en
Priority to EP00942322A priority patent/EP1246922B1/en
Priority to SK1889-2001A priority patent/SK18892001A3/en
Application filed by Basf Ag filed Critical Basf Ag
Priority to DE60045109T priority patent/DE60045109D1/en
Priority to AU57014/00A priority patent/AU783697B2/en
Priority to AT00942322T priority patent/ATE484590T1/en
Priority to CA002377378A priority patent/CA2377378A1/en
Priority to JP2001508355A priority patent/JP2003512024A/en
Priority to MXPA01012932A priority patent/MXPA01012932A/en
Priority to KR1020017016970A priority patent/KR20020025099A/en
Priority to BR0012038-3A priority patent/BR0012038A/en
Publication of WO2001002583A2 publication Critical patent/WO2001002583A2/en
Publication of WO2001002583A3 publication Critical patent/WO2001002583A3/en
Priority to AU2006200800A priority patent/AU2006200800A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1223—Phosphotransferases with a nitrogenous group as acceptor (2.7.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/03—Phosphotransferases with a nitrogenous group as acceptor (2.7.3)
    • C12Y207/03009—Phosphoenolpyruvate-protein phosphotransferase (2.7.3.9)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Isolated nucleic acid molecules, designated PTS nucleic acid molecules, which encode novel PTS proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing PTS nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated PTS proteins, mutated PTS proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of PTS genes in this organism.
PCT/IB2000/000973 1999-07-01 2000-06-27 Orynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins WO2001002583A2 (en)

Priority Applications (13)

Application Number Priority Date Filing Date Title
AU57014/00A AU783697B2 (en) 1999-07-01 2000-06-27 Orynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins
EP00942322A EP1246922B1 (en) 1999-07-01 2000-06-27 Corynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins
SK1889-2001A SK18892001A3 (en) 1999-07-01 2000-06-27 Corynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins
CA002377378A CA2377378A1 (en) 1999-07-01 2000-06-27 Corynebacterium glutamicum genes encoding phosphoenolpyruvate:sugar phosphotransferase system proteins
DE60045109T DE60045109D1 (en) 1999-07-01 2000-06-27 FOR PROTEINS OF PHOSPHOENOLPYRUVATE: SUGAR PHOSPHOTRANSFERASE SYSTEMS ENCODING GENES FROM CORYNEBACTERIUM GLUTAMICUM
HU0203191A HUP0203191A3 (en) 1999-07-01 2000-06-27 Corynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins
AT00942322T ATE484590T1 (en) 1999-07-01 2000-06-27 GENES FROM CORYNEBACTERIUM GLUTAMICUM CODING FOR PROTEINS OF THE PHOSPHOENOLPYRUVATE:SUGAR PHOSPHOTRANSFERASE SYSTEM
DE1246922T DE1246922T1 (en) 1999-07-01 2000-06-27 FOR PROTEINS OF THE PHOSPHOENOL PYRUVATE: SUGAR PHOSPHOTRANSFERASE SYSTEMS-GENES CODING FROM CORYNEBACTERIUM GLUTAMICUM
JP2001508355A JP2003512024A (en) 1999-07-01 2000-06-27 Corynebacterium glutamicum gene encoding phosphoenolpyruvate: sugar phosphotransferase protein
MXPA01012932A MXPA01012932A (en) 1999-07-01 2000-06-27 Orynebacterium glutamicum.
KR1020017016970A KR20020025099A (en) 1999-07-01 2000-06-27 Corynebacterium glutamicum genes encoding phosphoenolpyruvate:sugar phosphotransferase system proteins
BR0012038-3A BR0012038A (en) 1999-07-01 2000-06-27 Isolated nucleic acid molecule, vector, host cell, polopeptide, protein, and, methods for producing a polypeptide, for producing a fine chemical, and for diagnosing the presence or activity of corybacterium diphotheriae in a patient
AU2006200800A AU2006200800A1 (en) 1999-07-01 2006-02-24 Corynebacterium Glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US14269199P 1999-07-01 1999-07-01
US60/142,691 1999-07-01
US15031099P 1999-08-23 1999-08-23
US60/150,310 1999-08-23
DE19942097.1 1999-09-03
DE19942097 1999-09-03
DE19942095.5 1999-09-03
DE19942095 1999-09-03

Publications (2)

Publication Number Publication Date
WO2001002583A2 WO2001002583A2 (en) 2001-01-11
WO2001002583A3 true WO2001002583A3 (en) 2001-07-26

Family

ID=27438980

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2000/000973 WO2001002583A2 (en) 1999-07-01 2000-06-27 Orynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins

Country Status (15)

Country Link
EP (2) EP1246922B1 (en)
JP (3) JP2003512024A (en)
KR (2) KR20020025099A (en)
CN (2) CN1680559A (en)
AU (1) AU783697B2 (en)
BR (1) BR0012038A (en)
CA (1) CA2377378A1 (en)
CZ (1) CZ20014700A3 (en)
ES (1) ES2174768T1 (en)
HU (1) HUP0203191A3 (en)
MX (1) MXPA01012932A (en)
PL (1) PL359501A1 (en)
SK (1) SK18892001A3 (en)
TR (5) TR200700068T2 (en)
WO (1) WO2001002583A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60027161D1 (en) * 1999-07-02 2006-05-18 Ajinomoto Kk FOR SACCHAROSE-PTS-ENZYME II ENCODING DNA
DE10001101A1 (en) * 2000-01-13 2001-07-19 Degussa New nucleotide sequences coding for the ptsH gene
US6818432B2 (en) 2000-01-13 2004-11-16 Degussa Ag Nucleotide sequences encoding the ptsH gene
DE10045496A1 (en) 2000-09-14 2002-03-28 Degussa New nucleotide sequences coding for the ptsi gene
DE10154276A1 (en) * 2001-11-05 2003-05-15 Basf Ag Genes coding for phosphoenolpyruvate sugar phosphotransferase proteins
US7468262B2 (en) 2003-05-16 2008-12-23 Ajinomoto Co., Inc. Polynucleotides encoding useful polypeptides in corynebacterium glutamicum ssp. lactofermentum
ES2340405T3 (en) * 2004-09-28 2010-06-02 Kyowa Hakko Bio Co., Ltd. METHOD FOR PRODUCING L-ARGININE, L-ORNITINE OR L-CITRULINE.
EP1929028A1 (en) * 2005-09-27 2008-06-11 Ajinomoto Co., Inc. An l-amino acid-producing bacterium and a method for producing l-amino acids
US8048649B2 (en) * 2007-09-26 2011-11-01 Archer Daniels Midland Company Production of amino acids from sucrose in Corynebacterium glutamicum
WO2009154122A1 (en) * 2008-06-17 2009-12-23 財団法人地球環境産業技術研究機構 Coryneform bacterium transformant having improved d-xylose-utilizing function
JP5663859B2 (en) * 2009-11-13 2015-02-04 三菱化学株式会社 Non-amino organic acid producing bacteria and method for producing non-amino organic acid
EP3431599B1 (en) * 2016-03-16 2022-06-15 Kumiai Chemical Industry Co., Ltd. A tentoxin synthesis gene, a method for producing tentoxin or dihydrotentoxin using the same, and a transformant comprising the same
EP3529258A1 (en) * 2016-10-24 2019-08-28 Evonik Degussa GmbH Cells and method for producing rhamnolipids using alternative glucose transporters
US10633682B2 (en) 2017-03-07 2020-04-28 DePuy Synthes Products, Inc. Bioresorbable exopolysaccharides
WO2018165077A1 (en) 2017-03-07 2018-09-13 DePuy Synthes Products, Inc. Bioresorbable exopolysaccharides
CN112062821B (en) * 2020-09-24 2022-02-01 江南大学 Carbon catabolism regulatory protein CcpA mutant K31A
CN114763372A (en) * 2021-01-13 2022-07-19 中国科学院天津工业生物技术研究所 Protein with L-proline efflux function and application thereof

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EP0358940A1 (en) * 1988-09-12 1990-03-21 Degussa Aktiengesellschaft DNA fragment coding for phosphoenolpyruvat corboxylase, recombinant DNA carrying said fragment, strains carrying the recombinant DNA and method for producing L-aminino acids using said strains
WO1998018931A2 (en) * 1996-10-31 1998-05-07 Human Genome Sciences, Inc. Streptococcus pneumoniae polynucleotides and sequences

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EP0358940A1 (en) * 1988-09-12 1990-03-21 Degussa Aktiengesellschaft DNA fragment coding for phosphoenolpyruvat corboxylase, recombinant DNA carrying said fragment, strains carrying the recombinant DNA and method for producing L-aminino acids using said strains
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Also Published As

Publication number Publication date
AU5701400A (en) 2001-01-22
KR20060121993A (en) 2006-11-29
MXPA01012932A (en) 2002-07-30
TR200700069T2 (en) 2007-02-21
CA2377378A1 (en) 2001-01-11
HUP0203191A3 (en) 2009-01-28
JP2007267744A (en) 2007-10-18
JP2007236393A (en) 2007-09-20
KR20020025099A (en) 2002-04-03
CN1680559A (en) 2005-10-12
JP2003512024A (en) 2003-04-02
HUP0203191A2 (en) 2002-12-28
TR200403465T2 (en) 2005-03-21
SK18892001A3 (en) 2002-09-10
ES2174768T1 (en) 2002-11-16
TR200700067T2 (en) 2007-03-21
EP2272953A1 (en) 2011-01-12
EP2272953B1 (en) 2014-09-24
TR200103854T2 (en) 2002-06-21
PL359501A1 (en) 2004-08-23
WO2001002583A2 (en) 2001-01-11
EP1246922A2 (en) 2002-10-09
TR200700068T2 (en) 2007-03-21
BR0012038A (en) 2002-07-02
EP1246922B1 (en) 2010-10-13
CZ20014700A3 (en) 2002-06-12
CN1371420A (en) 2002-09-25
AU783697B2 (en) 2005-11-24

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