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WO2000077251A3 - Forensic dna profiling method comprising immunological separation step - Google Patents

Forensic dna profiling method comprising immunological separation step

Info

Publication number
WO2000077251A3
WO2000077251A3 PCT/GB2000/002203 GB0002203W WO2000077251A3 WO 2000077251 A3 WO2000077251 A3 WO 2000077251A3 GB 0002203 W GB0002203 W GB 0002203W WO 2000077251 A3 WO2000077251 A3 WO 2000077251A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
dna
epithelial
spermatozoa
sample
antibody
Prior art date
Application number
PCT/GB2000/002203
Other languages
French (fr)
Other versions
WO2000077251A2 (en )
Inventor
Matthew James Greenhalgh
Original Assignee
Astrazeneca Uk Ltd
Matthew James Greenhalgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay
    • G01N33/5308Immunoassay; Biospecific binding assay for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/689Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/02Assays, e.g. immunoassays or enzyme assays, involving carbohydrates involving antibodies to sugar part of glycoproteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00Detection or diagnosis of diseases
    • G01N2800/36Gynecology or obstetrics
    • G01N2800/367Infertility, e.g. sperm disorder, ovulatory dysfunction

Abstract

A DNA profiling method which comprises (i) contacting a sample believed to comprise spermatozoa and/or epithelial cells with antibodies specific for antigens presented on the spermatozoa and/or epithelial cells which antigens are not readily degraded, so as to form antibody-spermatozoa and/or antibody-epithelial cell complexes, (ii) separating the complex(es) from other material in the sample, (iii) isolating DNA from the complex(es) and (iv) determining the specific characteristics of the isolated DNA.
PCT/GB2000/002203 1999-06-11 2000-06-07 Forensic dna profiling method comprising immunological separation step WO2000077251A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB9913556.8 1999-06-11
GB9913556A GB9913556D0 (en) 1999-06-11 1999-06-11 Assays

Publications (2)

Publication Number Publication Date
WO2000077251A2 true WO2000077251A2 (en) 2000-12-21
WO2000077251A3 true true WO2000077251A3 (en) 2001-05-25

Family

ID=10855131

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/002203 WO2000077251A3 (en) 1999-06-11 2000-06-07 Forensic dna profiling method comprising immunological separation step

Country Status (2)

Country Link
GB (1) GB9913556D0 (en)
WO (1) WO2000077251A3 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7320891B2 (en) 2004-09-10 2008-01-22 Promega Corporation Methods and kits for isolating sperm cells
US8993292B2 (en) 2007-01-16 2015-03-31 Applied Biosystems Llc Methods and systems for differential extraction
EP2955521A1 (en) * 2014-06-11 2015-12-16 Centre Hospitalier Universitaire Vaudois (CHUV) Methods for separating cells

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0186271A1 (en) * 1984-11-12 1986-07-02 THE LISTER INSTITUTE OF PREVENTIVE MEDICINE Royal National Orthopaedic Hospital Method of characterising a test sample of genomic DNA
EP0238329A2 (en) * 1986-03-19 1987-09-23 Zeneca Limited Improvements in genetic probes
US4741998A (en) * 1985-06-05 1988-05-03 The University Of Virginia Alumni Patents Foundation Monoclonal antibody to MHS-5: a new probe for sexual assault analyses
US5047508A (en) * 1985-06-05 1991-09-10 The University Of Virginia Alumni Patents Foundation Monoclonal antibody to MHS-5; a new probe for sexual assault analysis
EP0530009A2 (en) * 1991-08-27 1993-03-03 Zeneca Limited Method of characterising genomic DNA
US5436157A (en) * 1989-03-03 1995-07-25 The University Of Virginia Alumni Patents Foundation Human intra-acrosomal sperm antigen
US5605803A (en) * 1989-03-03 1997-02-25 University Of Virginia Alumni Patents Foundation Human sperm diagnostic
WO1998028622A1 (en) * 1996-12-20 1998-07-02 Fodstad Oeystein Method for characterization of abnormal cells
US5830472A (en) * 1996-06-28 1998-11-03 The University Of Virginia Patent Foundation Purified sperm surface antigen, monoclonal antibody therefor and applications therefor
WO1999042129A1 (en) * 1998-02-23 1999-08-26 University Of Virginia Patent Foundation Purified sperm surface antigen, monoclonal antibody therefor and application therefor

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0186271A1 (en) * 1984-11-12 1986-07-02 THE LISTER INSTITUTE OF PREVENTIVE MEDICINE Royal National Orthopaedic Hospital Method of characterising a test sample of genomic DNA
US4741998A (en) * 1985-06-05 1988-05-03 The University Of Virginia Alumni Patents Foundation Monoclonal antibody to MHS-5: a new probe for sexual assault analyses
US5047508A (en) * 1985-06-05 1991-09-10 The University Of Virginia Alumni Patents Foundation Monoclonal antibody to MHS-5; a new probe for sexual assault analysis
EP0238329A2 (en) * 1986-03-19 1987-09-23 Zeneca Limited Improvements in genetic probes
US5436157A (en) * 1989-03-03 1995-07-25 The University Of Virginia Alumni Patents Foundation Human intra-acrosomal sperm antigen
US5605803A (en) * 1989-03-03 1997-02-25 University Of Virginia Alumni Patents Foundation Human sperm diagnostic
EP0530009A2 (en) * 1991-08-27 1993-03-03 Zeneca Limited Method of characterising genomic DNA
US5830472A (en) * 1996-06-28 1998-11-03 The University Of Virginia Patent Foundation Purified sperm surface antigen, monoclonal antibody therefor and applications therefor
WO1998028622A1 (en) * 1996-12-20 1998-07-02 Fodstad Oeystein Method for characterization of abnormal cells
WO1999042129A1 (en) * 1998-02-23 1999-08-26 University Of Virginia Patent Foundation Purified sperm surface antigen, monoclonal antibody therefor and application therefor

Also Published As

Publication number Publication date Type
WO2000077251A2 (en) 2000-12-21 application
GB9913556D0 (en) 1999-08-11 grant

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