WO2000076309A3 - Non-human transgenic animals deficient in gas6 function and their use - Google Patents

Non-human transgenic animals deficient in gas6 function and their use Download PDF

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Publication number
WO2000076309A3
WO2000076309A3 PCT/EP2000/005318 EP0005318W WO0076309A3 WO 2000076309 A3 WO2000076309 A3 WO 2000076309A3 EP 0005318 W EP0005318 W EP 0005318W WO 0076309 A3 WO0076309 A3 WO 0076309A3
Authority
WO
WIPO (PCT)
Prior art keywords
gas6
transgenic animals
human transgenic
animals deficient
function
Prior art date
Application number
PCT/EP2000/005318
Other languages
French (fr)
Other versions
WO2000076309A2 (en
Inventor
Peter Carmeliet
Desire Collen
Bjorn Dahlback
De Frutos Pablo Garcia
Cristina Aparicio
Original Assignee
Vlaams Interuniv Inst Biotech
Peter Carmeliet
Desire Collen
Bjorn Dahlback
De Frutos Pablo Garcia
Cristina Aparicio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vlaams Interuniv Inst Biotech, Peter Carmeliet, Desire Collen, Bjorn Dahlback, De Frutos Pablo Garcia, Cristina Aparicio filed Critical Vlaams Interuniv Inst Biotech
Priority to AU50771/00A priority Critical patent/AU5077100A/en
Priority to EP00935193A priority patent/EP1185635A2/en
Publication of WO2000076309A2 publication Critical patent/WO2000076309A2/en
Publication of WO2000076309A3 publication Critical patent/WO2000076309A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
    • A01K2267/0375—Animal model for cardiovascular diseases

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biochemistry (AREA)
  • Animal Husbandry (AREA)
  • Cardiology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

It has been shown that Gas6 deficient endothelial cells are resistant to activation by endotoxin and TNF-α in vitro and in vivo and that leukocytes did not or much less extravasate in Gas6 deficient mice. The present invention also relates to the manufacture of a medicament for the treatment of endothelial dysfunction. The present invention further relates to the use of an inhibitor of the Gas6 function and/or an inhibitor of a Gas6 receptor for the manufacture of said medicament.
PCT/EP2000/005318 1999-06-10 2000-06-08 Non-human transgenic animals deficient in gas6 function and their use WO2000076309A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU50771/00A AU5077100A (en) 1999-06-10 2000-06-08 Non-human transgenic animals deficient in gas6 function and their use
EP00935193A EP1185635A2 (en) 1999-06-10 2000-06-08 Non-human transgenic animals deficient in gas6 function and their use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99201859 1999-06-10
EP99201859.8 1999-06-10

Publications (2)

Publication Number Publication Date
WO2000076309A2 WO2000076309A2 (en) 2000-12-21
WO2000076309A3 true WO2000076309A3 (en) 2001-04-19

Family

ID=8240301

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/005318 WO2000076309A2 (en) 1999-06-10 2000-06-08 Non-human transgenic animals deficient in gas6 function and their use

Country Status (3)

Country Link
EP (1) EP1185635A2 (en)
AU (1) AU5077100A (en)
WO (1) WO2000076309A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030144237A1 (en) * 2000-04-13 2003-07-31 Peter Carmeliet Use of inhibition of a gas6 function or of a gas6 receptor for preventing and treating a cardiovascular disease
EP1199081A1 (en) * 2000-10-20 2002-04-24 Vlaams Interuniversitair Instituut voor Biotechnologie Use of inhibition of a growth arrest-specific gene (gas6) function or of a gas6 receptor for preventing and treating a thromboembolic disease
GB2374870A (en) * 2001-01-26 2002-10-30 Thromb X Nv Use of genetically modified multicellular animals in the identification of drug target proteins
DE10130657A1 (en) * 2001-06-27 2003-01-16 Axaron Bioscience Ag New endothetially expressed protein and its use
EP1382969A1 (en) * 2002-07-17 2004-01-21 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Diagnosis and prevention of cancer cell invasion
US8574827B2 (en) 2002-10-29 2013-11-05 Rigel Pharmaceuticals, Inc. Modulators of angiogenesis and tumorigenesis
RU2490632C1 (en) * 2012-05-17 2013-08-20 Государственное Бюджетное Образовательное Учреждение Высшего Профессионального Образования "Красноярский Государственный Медицинский Университет Имени Профессора В.Ф. Войно-Ясенецкого Министерства Здравоохранения И Социального Развития Российской Федерации" Method for stimulating endothelial dysfunction in vitro
GB201506411D0 (en) 2015-04-15 2015-05-27 Bergenbio As Humanized anti-axl antibodies
PL3612234T3 (en) 2017-04-20 2024-09-02 Adc Therapeutics Sa Combination therapy with an anti-axl antibody-drug conjugate

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1179A (en) * 1997-06-09 1999-01-06 Shionogi & Co Ltd Mouse having modified gas6 gene

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1179A (en) * 1997-06-09 1999-01-06 Shionogi & Co Ltd Mouse having modified gas6 gene

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FRIDELL YIH-WOEI C ET AL: "GAS6 induces Axl-mediated chemotaxis of vascular smooth muscle cells.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 12, 20 March 1998 (1998-03-20), pages 7123 - 7126, XP002154999, ISSN: 0021-9258 *
NAKANO TORU ET AL: "Requirement of gamma-carboxyglutamic acid residues for the biological activity of Gas6: Contribution of endogenous Gas6 to the proliferation of vascular smooth muscle cells.", BIOCHEMICAL JOURNAL, vol. 323, no. 2, 1997, pages 387 - 392, XP002155000, ISSN: 0264-6021 *
O'DONNELL KRISTY ET AL: "Expression of receptor tyrosine kinase Axl and its ligand Gas6 in rheumatoid arthritis: Evidence for a novel endothelial cell survival pathway.", AMERICAN JOURNAL OF PATHOLOGY, vol. 154, no. 4, April 1999 (1999-04-01), pages 1171 - 1180, XP000971169, ISSN: 0002-9440 *
PATENT ABSTRACTS OF JAPAN vol. 1999, no. 04 30 April 1999 (1999-04-30) *

Also Published As

Publication number Publication date
EP1185635A2 (en) 2002-03-13
AU5077100A (en) 2001-01-02
WO2000076309A2 (en) 2000-12-21

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