WO2000075362A3 - Method for identifying novel transcriptional regulatory proteins - Google Patents

Method for identifying novel transcriptional regulatory proteins Download PDF

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Publication number
WO2000075362A3
WO2000075362A3 PCT/IB2000/000859 IB0000859W WO0075362A3 WO 2000075362 A3 WO2000075362 A3 WO 2000075362A3 IB 0000859 W IB0000859 W IB 0000859W WO 0075362 A3 WO0075362 A3 WO 0075362A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
transcriptional regulatory
interest
transcription
regulatory proteins
Prior art date
Application number
PCT/IB2000/000859
Other languages
French (fr)
Other versions
WO2000075362A2 (en
Inventor
Wolfgang Hillen
Original Assignee
Wolfgang Hillen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wolfgang Hillen filed Critical Wolfgang Hillen
Priority to AU54177/00A priority Critical patent/AU5417700A/en
Publication of WO2000075362A2 publication Critical patent/WO2000075362A2/en
Publication of WO2000075362A3 publication Critical patent/WO2000075362A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for identifying transcriptional regulatory proteins that modulate the transcription of a gene of interest is disclosed. In one embodiment, the method comprises introducing into a cell a nucleic acid molecule comprising three central components: 1) a polynucleotide (e.g., DNA) encoding a transcriptional regulatory protein; 2) an indicator gene which is responsive to, e.g., under the transcriptional control of, the gene regulatory sequences of a gene of interest which bind to the transcriptional regulatory protein; and 3) a selectable marker gene. The transcriptional regulatory protein binds to the gene regulatory sequences and either activates or inhibits transcription. A protein is identified as a modulator of the transcription of the gene of interest by detecting a signal generated by the indicator gene.
PCT/IB2000/000859 1999-06-04 2000-06-05 Method for identifying novel transcriptional regulatory proteins WO2000075362A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU54177/00A AU5417700A (en) 1999-06-04 2000-06-05 Method for identifying novel transcriptional regulatory proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13767199P 1999-06-04 1999-06-04
US60/137,671 1999-06-04

Publications (2)

Publication Number Publication Date
WO2000075362A2 WO2000075362A2 (en) 2000-12-14
WO2000075362A3 true WO2000075362A3 (en) 2002-02-07

Family

ID=22478542

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2000/000859 WO2000075362A2 (en) 1999-06-04 2000-06-05 Method for identifying novel transcriptional regulatory proteins

Country Status (2)

Country Link
AU (1) AU5417700A (en)
WO (1) WO2000075362A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050266447A1 (en) * 2004-04-19 2005-12-01 Pioneer Hi-Bred International, Inc. Method for identifying activators of gene transcription

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040892A1 (en) * 1995-06-07 1996-12-19 Basf Ag Tetracycline regulated transcriptional modulators with altered dna binding specificities

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040892A1 (en) * 1995-06-07 1996-12-19 Basf Ag Tetracycline regulated transcriptional modulators with altered dna binding specificities

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BELLI G ET AL: "An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 4, 15 February 1998 (1998-02-15), pages 942 - 947, XP002176572 *
GOSSEN M ET AL: "Transcriptional activation by tetracyclines in mammalian cells.", SCIENCE, vol. 268, no. 5218, 1995, pages 1766 - 1769, XP000606285 *

Also Published As

Publication number Publication date
AU5417700A (en) 2000-12-28
WO2000075362A2 (en) 2000-12-14

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