WO2000075343A3 - ENGINEERING β-KETOACYL ACP SYNTHASE FOR NOVEL SUBSTRATE SPECIFICITY - Google Patents

ENGINEERING β-KETOACYL ACP SYNTHASE FOR NOVEL SUBSTRATE SPECIFICITY Download PDF

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Publication number
WO2000075343A3
WO2000075343A3 PCT/US2000/016151 US0016151W WO0075343A3 WO 2000075343 A3 WO2000075343 A3 WO 2000075343A3 US 0016151 W US0016151 W US 0016151W WO 0075343 A3 WO0075343 A3 WO 0075343A3
Authority
WO
WIPO (PCT)
Prior art keywords
ketoacyl
beta
substrate specificity
engineering
acp
Prior art date
Application number
PCT/US2000/016151
Other languages
French (fr)
Other versions
WO2000075343A2 (en
Inventor
Katayoon Dehesh
Dale Val
Original Assignee
Calgene Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc filed Critical Calgene Llc
Priority to CA002339517A priority Critical patent/CA2339517A1/en
Priority to EP00939815A priority patent/EP1114163A2/en
Priority to JP2001501626A priority patent/JP2003501063A/en
Publication of WO2000075343A2 publication Critical patent/WO2000075343A2/en
Publication of WO2000075343A3 publication Critical patent/WO2000075343A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Methods of altering substrate specificity of beta-ketoacyl-ACP synthase, and engineered beta-ketoacyl-ACP synthases so produced are provided. DNA sequences and constructs for expression of engineered beta-ketoacyl-ACP synthases, as well as the novel beta-ketoacyl-ACP synthases produced therefrom are also provided. Such DNA sequences may be used for expression of the engineered beta-ketoacyl-ACP synthases in host cells, particularly seed cells of oilseed crop plants, for the modification of fatty acid composition.
PCT/US2000/016151 1999-06-09 2000-06-09 ENGINEERING β-KETOACYL ACP SYNTHASE FOR NOVEL SUBSTRATE SPECIFICITY WO2000075343A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002339517A CA2339517A1 (en) 1999-06-09 2000-06-09 Engineering .beta.-ketoacyl acp synthase for novel substrate specificity
EP00939815A EP1114163A2 (en) 1999-06-09 2000-06-09 Engineering beta-ketoacyl acp synthase for novel substrate specificity
JP2001501626A JP2003501063A (en) 1999-06-09 2000-06-09 Processing of β-ketoacyl ACP synthase for novel substrate specificity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13830899P 1999-06-09 1999-06-09
US60/138,308 1999-06-09

Publications (2)

Publication Number Publication Date
WO2000075343A2 WO2000075343A2 (en) 2000-12-14
WO2000075343A3 true WO2000075343A3 (en) 2001-04-05

Family

ID=22481450

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/016151 WO2000075343A2 (en) 1999-06-09 2000-06-09 ENGINEERING β-KETOACYL ACP SYNTHASE FOR NOVEL SUBSTRATE SPECIFICITY

Country Status (4)

Country Link
EP (1) EP1114163A2 (en)
JP (1) JP2003501063A (en)
CA (1) CA2339517A1 (en)
WO (1) WO2000075343A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR013633A1 (en) 1997-04-11 2001-01-10 Calgene Llc METHOD FOR THE ALTERATION OF THE COMPOSITION OF AVERAGE CHAIN FAT ACIDS IN VEGETABLE SEEDS THAT EXPRESS A THIOESTERASE THAT PREFERS HETEROLOGICAL VEGETABLE AVERAGE CHAIN.
EP1303616A2 (en) 2000-07-25 2003-04-23 Calgene LLC Nucleic acid sequences encoding beta-ketoacyl-acp synthase and uses thereof
WO2016179572A1 (en) 2015-05-07 2016-11-10 William Marsh Rice University Alpha omega bifunctional fatty acids
JP2016214183A (en) * 2015-05-22 2016-12-22 花王株式会社 Method for producing lipid
WO2024103060A1 (en) * 2022-11-11 2024-05-16 Ingateygen Llc Methods and compositions for modifying peanut seed oil

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996036719A1 (en) * 1995-05-15 1996-11-21 Calgene, Inc. Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity
WO1998046776A2 (en) * 1997-04-11 1998-10-22 Calgene Llc Plant fatty acid synthases and use in improved methods for production of medium-chain fatty acids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996036719A1 (en) * 1995-05-15 1996-11-21 Calgene, Inc. Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity
WO1998046776A2 (en) * 1997-04-11 1998-10-22 Calgene Llc Plant fatty acid synthases and use in improved methods for production of medium-chain fatty acids

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ABBADI AMINE ET AL: "Reaction mechanism of recombinant 3-oxoacyl-(acyl-carrier-protein) synthase III from Cuphea wrightii embryo, a fatty acid synthase type II condensing enzyme.", BIOCHEMICAL JOURNAL., vol. 345, no. 1, 1 January 2000 (2000-01-01), pages 153 - 160, XP000960950, ISSN: 0264-6021 *
DEHESH ET AL: "KAS IV: a 3-ketoacyl-ACP synthase form Cuphea sp. is a medium chain specific condensing enzyme", PLANT JOURNAL,GB,BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, vol. 15, no. 3, 1 August 1998 (1998-08-01), pages 383 - 390, XP002081435, ISSN: 0960-7412 *
EDWARDS PATRICIA ET AL: "Cloning of the fabF gene in an expression vector and in vitro characterization of recombinant fabF and fabB encoded enzymes from Escherichia coli.", FEBS LETTERS, vol. 402, no. 1, 1997, pages 62 - 66, XP002151732, ISSN: 0014-5793 *
LEONARD ET AL: "A Cuphea beta-ketoacyl-ACP synthase shifts the synthesis of fatty acids towards shorter chains in Arabidopsis seeds expressing Cuphea FatB thioesterases", PLANT JOURNAL,GB,BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, vol. 13, no. 5, 1 March 1998 (1998-03-01), pages 621 - 628, XP002081429, ISSN: 0960-7412 *
MOCHE MARTIN ET AL: "Structure of the complex between the antibiotic cerulenin and its target, beta-ketoacyl-acyl carrier protein synthase.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 10, 5 March 1999 (1999-03-05), pages 6031 - 6034, XP002151731, ISSN: 0021-9258 *
VAL D ET AL: "Re-engineering ketoacyl synthase specificity.", STRUCTURE (LONDON), vol. 8, no. 6, 2000, pages 565 - 566, XP000961320, ISSN: 0969-2126 *
WITKOWSKI ANDRZEJ ET AL: "Conversion of a beta-ketoacyl synthase to a malonyl decarboxylase by replacement of the active-site cysteine with glutamine.", BIOCHEMISTRY, vol. 38, no. 36, pages 11643 - 11650, XP002151733, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
EP1114163A2 (en) 2001-07-11
CA2339517A1 (en) 2000-12-14
WO2000075343A2 (en) 2000-12-14
JP2003501063A (en) 2003-01-14

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