WO2000073470A2 - Polynucleotides endo- et exo-glucanase et leurs procedes d'utilisation - Google Patents

Polynucleotides endo- et exo-glucanase et leurs procedes d'utilisation Download PDF

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Publication number
WO2000073470A2
WO2000073470A2 PCT/US1999/012761 US9912761W WO0073470A2 WO 2000073470 A2 WO2000073470 A2 WO 2000073470A2 US 9912761 W US9912761 W US 9912761W WO 0073470 A2 WO0073470 A2 WO 0073470A2
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WO
WIPO (PCT)
Prior art keywords
plant
polynucleotide
sequence
glucanase
endo
Prior art date
Application number
PCT/US1999/012761
Other languages
English (en)
Inventor
Carl R. Simmons
Original Assignee
Pioneer Hi-Bred International, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi-Bred International, Inc. filed Critical Pioneer Hi-Bred International, Inc.
Priority to AU44258/99A priority Critical patent/AU4425899A/en
Publication of WO2000073470A2 publication Critical patent/WO2000073470A2/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des polynucléotides endo- et exo-glucanase isolés ainsi que des compositions et des procédés destinés à la modulation (augmentation ou réduction, par exemple) des niveaux totaux d'une protéine endo- ou exo-glucanase et/ou à la modification de leurs rapports dans des plantes. La présente invention concerne également des acides nucléiques isolés suffisamment longs et complémentaires d'un polynucléotide endo-ou exo-glucanase pour être utilisés comme sondes ou comme amorceurs d'amplification afin de détecter, de quantifier ou d'isoler des transcrits génétiques. La modulation de protéines endo- ou exo-glucanase peut être destinée à différents types d'utilisations, et en particulier - mais pas uniquement - à l'élongation ou à l'expansion de parois cellulaires, d'où une modification de la croissance d'une plante ou une amélioration de la vitesse de croissance de ses grains, une augmentation de la digestibilité des plantes d'ensilage ou de fourrage, une amélioration de la résistance des plantes aux agents pathogènes et aux stress, et une modification dans la floraison et la maturation des fruits et des grains, dans l'abscission et la sénescence et dans la différentiation tissulaire.
PCT/US1999/012761 1999-05-26 1999-06-08 Polynucleotides endo- et exo-glucanase et leurs procedes d'utilisation WO2000073470A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU44258/99A AU4425899A (en) 1999-05-26 1999-06-08 Endo- and exo-glucanase polynucleotides and methods of use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32007699A 1999-05-26 1999-05-26
US09/320,076 1999-05-26

Publications (1)

Publication Number Publication Date
WO2000073470A2 true WO2000073470A2 (fr) 2000-12-07

Family

ID=23244774

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/012761 WO2000073470A2 (fr) 1999-05-26 1999-06-08 Polynucleotides endo- et exo-glucanase et leurs procedes d'utilisation

Country Status (2)

Country Link
AU (1) AU4425899A (fr)
WO (1) WO2000073470A2 (fr)

Also Published As

Publication number Publication date
AU4425899A (en) 2000-12-18

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