WO2000072009A1 - Device for the collection and analysis of body secretions - Google Patents

Device for the collection and analysis of body secretions Download PDF

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Publication number
WO2000072009A1
WO2000072009A1 PCT/FR2000/001213 FR0001213W WO0072009A1 WO 2000072009 A1 WO2000072009 A1 WO 2000072009A1 FR 0001213 W FR0001213 W FR 0001213W WO 0072009 A1 WO0072009 A1 WO 0072009A1
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WO
WIPO (PCT)
Prior art keywords
analysis
sampling
zone
cups
fluids
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PCT/FR2000/001213
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French (fr)
Inventor
Hemery Fernand Moutete
Original Assignee
Hemery Fernand Moutete
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Publication date
Application filed by Hemery Fernand Moutete filed Critical Hemery Fernand Moutete
Priority to EP00925395A priority Critical patent/EP1097376A1/en
Publication of WO2000072009A1 publication Critical patent/WO2000072009A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A61B10/007—Devices for taking samples of body liquids for taking urine samples
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B2010/0003—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements including means for analysis by an unskilled person
    • A61B2010/0006—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements including means for analysis by an unskilled person involving a colour change

Definitions

  • the present invention relates to a device for collecting and analyzing bodily secretions from the urogenital region, particularly in women.
  • the present invention aims in particular at the diagnosis of low urogenital infections. It may also find applications in the field of monitoring weight-loss programs, monitoring the effectiveness of slimming products, or even monitoring antibiotic therapies.
  • Low urogenital infections are localized infections of the vagina, vulva and cervix.
  • the infectious agents to be detected are, in particular: Candida albicans, Chlamydia trachomatis, Corynebacterium vaginalis, Enterobacteriaceae, Enterococcus, Esche ⁇ chia coli, Gardnerella vaginalis, Haemophilus vaginalis, Klebsiella, Mycoplasma hominis, Neisseria gonorrhoeae, Trichomonas vagonasum, Trichomonas vagina , Proteus mirabilis, Staphylococcus aureus, Streptococcus beta-hemolytic, Ureaplasma urealyticum.
  • the chemical mechanism involves the formation of specific bio bonds with the compounds to be detected and makes it possible to measure the following parameters with regard to urinary and / or vaginal infection: leukocyte esterase (leukocyturia), nitrites, pH, albumin, glucose, ketone bodies, urobilinogens, bilirubin, hemoglobin (hematuria).
  • leukocyte esterase leukocyturia
  • nitrites pH, albumin, glucose, ketone bodies
  • urobilinogens bilirubin
  • hemoglobin hematuria
  • the micro biological analysis method performs the detection of the above infectious agents in selective culture media containing a substrate metabolizable by the microorganism, growth activators, antibiotics and chromogenic molecules which allow the colorimetric identification of the pathogen. Generally, this reading is carried out in the laboratory using a spectrophotometer.
  • probes for example, intended for taking samples of urogenital fluids. These probes are generally used to carry out only the sampling, possibly the transfer of the fluid to be analyzed to the analysis medium or media, generally contained in flasks, test tubes or the like, as described in document EP237394.
  • a disadvantage of these procedures is that the substance to be analyzed can be contaminated, or undergo physicochemical or bacteriological modifications by the preservatives during the pre-analytical period, thus distorting the final analysis.
  • this type of sample requires a gynecological procedure which can be unpleasant for the patient, and at the very least restrictive in relation to her current activity.
  • a sanitary napkin of design adapted to such a sampling, integrating a sampling strip or a sampling filter.
  • This device is intended to facilitate the taking of samples, but does not solve the problems of delays and errors of analysis, because it in fact only serves to collect secretions, and to facilitate their transfer to place of analysis.
  • the multiple influences of sampling conditions on the reliability and accuracy of the results cannot be avoided, and in particular the influence of the time between sampling and analysis remains on the viability of microorganisms or the alkalization of urine.
  • the sampling operation alone already poses many binding problems on the methodology and the means to be used, to which are added the problems means of transport and storage, the influence of preservatives on the methods and results of the analysis, time before and for analysis, commonly performed in the laboratory, and time to return the results to the patient or to his doctor, etc.
  • These methods require equipment and trained personnel, have fairly long response times, and are expensive.
  • the usual sampling methods can cause considerable analytical variations, which could call into question the accuracy and reliability of the test results.
  • the present invention aims to provide a solution to the problems mentioned above and aims in particular to provide a means of rapid detection of urinary tract and genital infections, which is comfortable to use and allows early, rapid and most rapid diagnosis. reliable possible by the user herself, while remaining very easy to use, and of a fairly low cost.
  • the invention aims to allow direct and rapid analyzes of chemical parameters and infectious germs from bodily fluids taken from specific sites in the female urogenital region. These direct analyzes relate to the detection of chemical indices of infection, the enumeration and the identification of pathogenic microorganisms.
  • the invention also aims to provide a product for ensuring easy monitoring of a weight loss treatment, or a control of the effectiveness of slimming products, by assaying the ketone bodies present in the fluids collected. Still other objectives are to enable monitoring of antibiotic therapy, or to provide medical assistance for procreation or monitoring the progress of pregnancy by assaying steroid hormones such as progesterone and gonadotrophic hormone cho ⁇ onique (hCG). .
  • steroid hormones such as progesterone and gonadotrophic hormone cho ⁇ onique (hCG).
  • the invention relates to a device for collecting and analyzing bodily secretions from the urogenital region, comprising an absorbent support adapted to be brought, by a first face, into intimate contact with the body in the urogenital region and comprising means for withdrawing bodily fluids for their analysis, characterized in that it comprises, integrates into the volume of the absorbent support, means for in-situ analysis of said fluids and display means for viewing the results of the analysis on its surface.
  • the visualization will be done on the face opposite to said first face.
  • the absorbent article according to the invention allows, thanks to suitable analysis means, the detection and direct diagnosis of the chemical parameters and / or of the microorganisms responsible for low urogenital infections. It preferably comes in the form of feminine protection such as a sanitary napkin, panty liner, tampon, or even in the form of a diaper for children or a layer for incontinence.
  • an upper absorbent layer of contact with the urogenital region comprising at least one site for sampling bodily fluids or samples to be analyzed, - chemical reaction media or culture media, constituting the said means of analysis, layers and absorbent and diffusing conduits for bringing the bodily fluids collected to the sampling sites to the so-called chemical reaction media or culture media, then to an overflow storage area, an external waterproof and at least locally transparent film allowing a visual reading of the results on the visualization means.
  • Each culture medium or reactive zone contains a chromogen and / or colored indicator which detects the modifications of the medium caused by the metabolism of microorganisms which signals an index of infection or an identification of the pathogenic agent.
  • the device will include conventionally three sampling sites, for taking samples of urine, vaginal secretions and anal secretions respectively. In other applications, one or two sampling sites may suffice.
  • each sampling site corresponds to a specific sampling zone on the surface of which are distributed analysis cups containing the analysis means, in direct contact with the sampling zone, the bottom of the cups flush with the external film. .
  • distribution channels extend over the surface of the sampling zone, between the analysis wells.
  • the sampling zone communicates by its periphery with overflow discharge conduits which are themselves in communication with the overflow storage zone which is located between the analysis cups and the external film and isolated from the sampling area;
  • each sampling zone has on its surface an impermeable film in which an opening is provided at the sampling site, and an absorbent recovery band covers all of the impermeable films and is in communication by its periphery with the storage zone overflow;
  • the principle of the feminine protective towel according to the invention is based on the fact that it absorbs physiological liquids and diffuses them over the entire surface comprising the reactive zones corresponding to a sampling site concerned.
  • the fluids captured by a sampling site will be able to diffuse on the surface of the corresponding sampling zone and reach the different reactive zones of this sampling zone, but will not be able to diffuse from one sampling zone to another, to avoid mixtures of fluids.
  • the overflows of each sampling zone will be channeled towards the overflow storage zone, insulated and sealed from the sampling zones and the reactive zones, where the fluids can mix without discomfort, and which will therefore ensure maximum absorption capacity.
  • An advantage of feminine protection of the sanitary napkin type is that it constitutes an almost ideal support, the implementation of which is particularly easy. Placed and maintained in direct contact with the body for the period necessary for the analysis, it makes it possible to directly absorb physiological fluids and bring them into contact with the reagents without delay and also as directly as possible, minimizing the risks of exogenous contamination of fluids subjected to analysis. In addition it allows to respond to classic characteristics required of female protections, namely maximum absorption of body fluids, obtained thanks to the channeling and accumulation areas of the overflow, which evacuate in the thickness of the towel, as for a conventional sanitary napkin, the volumes of fluids exceeding what is necessary to supply the reactive zones.
  • the conventional dimensions of the female protections make it possible to place the reactive elements therein, even in number, fairly easily and to provide a surface on which the visualization of the results can be easily carried out and observed, giving great ease of diagnosis without that it is necessary to make specific samples.
  • the u ⁇ naires examinations first use chemical detection techniques, taking into account their speed and the nature of the urine which is free from microorganisms in the normal state, these techniques constituting a good orientation diagnostic support.
  • examinations of urogenital secretions are more difficult to perform due to the presence of a commensal flora coexisting with the purely pathogenic flora.
  • use is preferably made of biochemical techniques on selective culture media, which make it possible to identify the infectious agents at the origin of the pathology.
  • a product model could be used to provide a method of rapid chemical detection, in approximately 30 minutes, using clues of urinary tract infection; and another model will propose a semi-rapid micro-biological method using culture media making it possible to identify the pathogenic agent.
  • the detection of low urogenital infections according to the invention is a rapid, simple and precise method, which makes it possible to obtain a response in a particularly short time, of less than an hour.
  • the preferred type of culture medium comprises a nutrient base necessary for the development of micro ⁇ organisms, of type known per se, a growth enhancer, a chromogenic or fluorogenic substrate and one or more antibiotics inhibit ⁇ unwanted growth of micro organisms in the culture medium.
  • the chromogenic or fluorogenic substrates are those conventionally used for the detection of microorganisms.
  • a substrate having a hydrolysis intensity linked to a strong coloration visible to the naked eye will be chosen, a maximum enzymatic affinity for the activity to be detected and good solubility which limits the signal to the immediate vicinity of the colonies.
  • the amplification of the signal by release of the chromophore or fluorophore group is visualized without requiring the use of a device such as a UV lamp or a spectrophotometer.
  • Another variant of the colorimetric identification method well known to those skilled in the art, consists in introducing into the culture medium substances which detect the slight changes generated by the growth of microorganisms.
  • activators can be added to the culture medium to potentiate the enzymatic activity to be detected. They may be hexosamines, bivalent cationic compound salts such as the salts of Mn 2+ , Mg 2+ , Ca 2 X
  • Permeabilization agents or surfactants can also be added to promote better enzyme-substrate bonding.
  • the culture medium contains at least one inhibitor of microorganisms not sought.
  • This inhibitor in the presence of other constituents of the medium, including the chromogenic compounds, makes it possible to obtain a culture medium entirely specific to the kind of microorganism sought.
  • the culture medium allows specific detection as well as the counting of the microorganisms sought.
  • FIG. 1 is a plan view of the towel illustrating the arrangement of the different elements which constitute it, it being understood that these different elements are integrated into the thickness of the towel and therefore not really visible from the outside;
  • FIG. 2 is a cross-sectional view, on an enlarged scale, along the line II-II of FIG. 1
  • FIG. 3 is a partial view in longitudinal section along the line III-III of FIG. 1
  • FIG. 4 is a very schematic view in longitudinal section illustrating the juxtaposition of the three sampling zones
  • FIG. 5 is a side view of an alternative embodiment, intended to improve the accuracy of the location of the samples
  • each zone has an upper bulge corresponding, as will be seen below, to the sampling sites 110, 120, 130.
  • bulges correspond respectively to the location of the unary meatus for the detection zone 11, from the vaginal canal for the detection zone 12, and to the anus for the detection zone 13. They make it possible to ensure optimal contact with the parts of the body which are the origins of the secretions to be analyzed.
  • the particular shape of the suction cup given to the sampling sites aims to ensure even closer and above all very localized contact with the sources of the secretions, in particular for carrying out very localized sampling, and thus avoiding as much as possible disturbances that could be caused by absorption in the secretion collection areas contaminants from the periphery of the secretion sources to be analyzed.
  • the detection device 1 comprises an envelope constituted, on the side of the upper face 2 intended to be placed in contact with the body, by a contact veil 21, of soft and porous material, covering the entire upper surface, and on the side opposes 3, downwards, an impermeable film 22, for example a thin film of plastic material, resistant to traction and to chemicals, which is connected to the contact veil by enveloping the periphery of the towel, as is see Figure 2, and which seals the device to the outside.
  • a contact veil 21 of soft and porous material, covering the entire upper surface, and on the side opposes 3, downwards, an impermeable film 22, for example a thin film of plastic material, resistant to traction and to chemicals, which is connected to the contact veil by enveloping the periphery of the towel, as is see Figure 2, and which seals the device to the outside.
  • each sampling zone is formed of an absorbent layer 27, for example of pure cotton (constituted by a succession of cellulose patterns) or of another material based on cellulose, cellulose acetate or other derivatives, or else d 'other highly absorbent materials offering good resistance and porosity characteristics, in relatively reduced thicknesses.
  • Each absorbent layer 27 rests on a flexible plate 28 of impermeable material, shaped so as to have a plurality of analysis cups 29 of truncated shape and which extend up to the film. sealed 22.
  • Each well 29 contains a chemical reagent or a reaction medium specifically intended to ensure the detection of an infectious agent.
  • the bottom of each well is in contact with the waterproof and transparent film 22, which thus makes it possible to visualize the changes in color in the wells caused by the reaction of the chemical reagent or of the culture medium with the fluids withdrawn and directed towards the wells.
  • the absorbent layer 27 ensures by capillarity the transfer of the fluids captured through the opening 26 towards the chemical reaction media or culture media predisposed in the wells.
  • the ratio of the total surface of the absorbent layer 27 to the total surface of the openings of the wells is high, which promotes the penetration of fluids captured in the wells and the rapid filling of the wells, even those located furthest from the sampling site.
  • This filling can also be favored by the existence of a vacuum provided for this purpose in the analysis wells, which also prevents the phenomena of overloading or dilution of the reagents.
  • diffusion channels 30 are preferably made on the surface of the plate 28 to accelerate and homogenize the distribution of the fluids over the entire surface of the plate and therefore in the various analysis wells.
  • the analysis wells preferably have a frustoconical shape favorable to better contact of fluids with the reaction medium which they contain.
  • the reaction chamber formed by the bottom of the wells 29 will preferably be designed so as not to be completely full and to retain an air bubble which promotes mixing of the reaction medium.
  • the addition of body fluid is achieved by direct aspiration in the reaction chamber which contains the reagents necessary for carrying out the reactions for detecting infectious agents.
  • These wells are preferably made of a material such as polypropylene which ensures good heat distribution and maintains the temperature around 37 ° C. in the wells, which constitutes a micro-incubator necessary for the growth of microorganisms and upon detection.
  • absorbent and diffusing strips or conduits 31, 32 are arranged, in contact with the edges of the absorbent layer 27, to redirect the fluid overflow to the overflow storage area 23 , consisting of a very absorbent material distributed between the plate 28 and the film 22, in the intervals between the cups 29.
  • the diffusion channels 30 preferably extend longitudinally to distribute the fluids as well as possible, and open into the excess conduits -full 32, as seen in Figure 1.
  • Holding cups 33 are preferably arranged in the overflow storage zone, between the analysis cups, as seen in FIGS. 2 and 3. These cups can also be produced in the form of a preformed plate as plate 28. They are also tapered, inverted with respect to the analysis cups, pierced and filled with a very absorbent material. In addition to their participation in the collection of the overflow, they give a certain rigidity to the device by preventing its crushing and therefore the deformation of the analysis cups, which ensures the maintenance of the initial reaction volume and therefore the accuracy and reproducibility of the results of the analyzes.
  • the impermeable film 25 extends over and around the sampling zones, to border them outwards and separate them from one another. It thus avoids a mixing by diffusion of fluids from neighboring sampling sites. It also avoids mixing of the contaminating fluids captured by the recovery band 24 with the fluids to be analyzed captured by the absorbent layers 27.
  • the band 24 extends along the edges of the napkin by forming peripheral drainage channels 34 which transfer the secretions contaminating towards the side overflow strips 31 and the overflow storage area 23.
  • 6 to 8 show by way of illustration various possibilities, in no way limiting, of arrangements of display means on the side of the lower face , that is to say the one on which the results of the analyzes are made visible by change of color of the reagents contained in the wells 29, the change in color being perceived through the bottom 35 of the wells and of the external film 22.
  • FIG. 6 shows for example a set of ten indicator zones 35 corresponding to ten chemical type analyzes, that is to say rapid tests, for example leukocyte esterase, nitrites, pH, albumin, glucose, ketone bodies, proteins, hemoglobin, bilirubin, urobilmogene.
  • FIG. 6 shows for example a set of ten indicator zones 35 corresponding to ten chemical type analyzes, that is to say rapid tests, for example leukocyte esterase, nitrites, pH, albumin, glucose, ketone bodies, proteins, hemoglobin, bilirubin, urobilmogene.
  • FIG. 7 similarly shows the indicator zones 35 in the case of semi-rapid tests for the detection of infectious agent, such as for example: Esche ⁇ chia coli, Neisse ⁇ a gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Yeasts, Herpes simplex, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa.
  • infectious agent such as for example: Esche ⁇ chia coli, Neisse ⁇ a gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Yeasts, Herpes simplex, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa.
  • FIG. 8 illustrates the case of a device used for the detection of chemical slimming indices, by presenting colored indicators of: acetone, aceto-acetic acid, beta-hydroxybuty ⁇ que acid, glucose, creatinme.
  • the invention is not limited to the embodiment described above solely by way of example, and the technology of embodiment can be modified and adapted without departing from the scope of the invention as defined by the appended claims.
  • a person skilled in the art can easily adapt the topology and the nature of the reagents to detect other pathologies or physiological states, for example: detection of chemical indicators of thinness, osteoporosis, nephropathy, myopathies, detection of infectious diseases (typhoid fever, measles, tuberculosis, pneumonia, general sarcomas or Hodgkin's disease), detection of intoxication (lead, phosphorus, mercury, cadmium, uranium, bismuth), avitaminosis, detection of mental retardation , detection of bone tumors, hyperparathyroidism, prenatal diagnosis of congenital malformations, diagnosis of pregnancy, medically assisted procreation, monitoring of the progress of pregnancy, etc.

Abstract

A device for the collection and analysis of body secretions from the urogenital region, comprising a first absorbent support which is adapted in such a way that it can be placed, by means of a first surface (2), in intimate contact with the body in the urogenital region and comprising means (27) for taking a sample of body fluids with a view to the analysis thereof. The invention is characterized in that it includes means (29) for in-situ analysis of said fluids which are integrated into the volume of the absorbent support, and display means (34) for visualization of the results of the analysis on the surface thereof.

Description

DISPOSITIF DE RECUEIL ET D'ANALYSE DES SECRETIONS CORPORELLES DEVICE FOR COLLECTING AND ANALYZING BODILY SECRETIONS
La présente invention concerne un dispositif de recueil et d'analyse des sécrétions corporelles de la région urogenitale, particulièrement chez la femme.The present invention relates to a device for collecting and analyzing bodily secretions from the urogenital region, particularly in women.
La présente invention vise notamment le diagnostic des infections urogénitales basses. Elle pourra aussi trouver des applications dans le domaine du suivi de cures d'amaigrissement, du contrôle de l' efficacité de produits amincissants, ou encore du suivi d ' antibiothérapies . Les infections urogénitales basses sont les infections localisées au niveau du vagin, de la vulve et du col de l'utérus. Les agents infectieux à détecter sont notamment : Candida albicans, Chlamydia trachomatis, Corynebacterium vaginalis, Entérobactéries, Entérococcus, Escheπchia coli, Gardnerella vaginalis, Haemophilus vaginalis, Klebsiella, Mycoplasma hominis, Neisseria gonorrhoeae, Trichomonas vaginalis, gonocoque, Levures, Herpès simplex, Pseudomonas aerugmosa, Proteus mirabilis, Staphylococcus aureus, Streptocoque beta- hemolytique, Ureaplasma urealyticum.The present invention aims in particular at the diagnosis of low urogenital infections. It may also find applications in the field of monitoring weight-loss programs, monitoring the effectiveness of slimming products, or even monitoring antibiotic therapies. Low urogenital infections are localized infections of the vagina, vulva and cervix. The infectious agents to be detected are, in particular: Candida albicans, Chlamydia trachomatis, Corynebacterium vaginalis, Enterobacteriaceae, Enterococcus, Escheπchia coli, Gardnerella vaginalis, Haemophilus vaginalis, Klebsiella, Mycoplasma hominis, Neisseria gonorrhoeae, Trichomonas vagonasum, Trichomonas vagina , Proteus mirabilis, Staphylococcus aureus, Streptococcus beta-hemolytic, Ureaplasma urealyticum.
Le dépistage et le traitement préventif des infections uπnaires et génitales se heurtent a divers problèmes tenant notamment, pour ce qui concerne les analyses d'urine, au risque de contamination exogène lors du prélèvement, mais aussi au délai entre prélèvement et analyses et au délai de réalisation des analyses elles- mêmes. En ce qui concerne les infections génitales, s'y ajoutent la lourdeur des procédures de prélèvement et la réticence des patientes a s'y soumettre. Deux méthodes particulières d'analyse sont classiquement utilisées. La méthode d'analyse chimique repose sur la mesure de l'activité de certains métabolites libérés par les micro-organismes pathogènes, les hématies et leucocytes présents dans les urines ou les sécrétions vaginales. Le mécanisme chimique fait intervenir la formation de liaisons bio spécifiques avec les composés à détecter et permet de mesurer les paramètres suivants en ce qui concerne l'infection urinaire et/ou vaginale : estérase leucocytaire (leucocyturie) , nitrites, pH, albumine, glucose, corps cétoniques, urobilinogènes, bilirubine, hémoglobine (hématurie) .Screening and preventive treatment of uπnital and genital infections come up against various problems including, with regard to urine analyzes, the risk of exogenous contamination during sampling, but also the delay between sampling and analyzes and the delay of carrying out the analyzes themselves. With regard to genital infections, there is the cumbersome sampling procedures and the reluctance of patients to submit to them. Two particular methods of analysis are conventionally used. The chemical analysis method is based on the measurement of the activity of certain metabolites released by pathogenic microorganisms, red blood cells and leukocytes present in the urine or vaginal secretions. The chemical mechanism involves the formation of specific bio bonds with the compounds to be detected and makes it possible to measure the following parameters with regard to urinary and / or vaginal infection: leukocyte esterase (leukocyturia), nitrites, pH, albumin, glucose, ketone bodies, urobilinogens, bilirubin, hemoglobin (hematuria).
La méthode d'analyse micro biologique réalise la détection des agents infectieux ci-dessus dans des milieux sélectifs de culture contenant un substrat metabolisable par le micro-organisme, des activateurs de croissance, des antibiotiques et des molécules chromogènes qui permettent l'identification colorimétrique de l'agent pathogène. Généralement, cette lecture est effectuée en laboratoire à l'aide de spectrophotomètre .The micro biological analysis method performs the detection of the above infectious agents in selective culture media containing a substrate metabolizable by the microorganism, growth activators, antibiotics and chromogenic molecules which allow the colorimetric identification of the pathogen. Generally, this reading is carried out in the laboratory using a spectrophotometer.
D'autres méthodes ont été développées plus récemment, par exemple les méthodes immuno-enzymatiques et les méthodes de biologie moléculaire par amplification du génome du micro-organisme. Toutes ces méthodes impliquent cependant de manière générale la succession d'étapes suivantes : préparation de la patiente, recueil des échantillons à analyser, préparation de ces échantillons (adjonction d'agents conservateurs, centrifugation, etc.), prise en compte des délais d'analyse, transport des échantillons vers le laboratoire d'analyse, exécution de l'analyseOther methods have been developed more recently, for example immuno-enzymatic methods and methods of molecular biology by amplification of the genome of the microorganism. All these methods however generally involve the following sequence of steps: preparation of the patient, collection of samples for analysis, preparation of these samples (addition of preservatives, centrifugation, etc.), taking into account analysis, transport of samples to the analysis laboratory, execution of the analysis
(ensemencement, enrichissement, sélection, etc.), interprétation des résultats, et transmission à la patiente. Cette succession d'opérations rend ces méthodes lourdes d'exécution, avec des délais de réponse longs, d'où inconvénients ma eurs pour la prise en charge thérapeutique et/ou prophylactique.(seeding, enrichment, selection, etc.), interpretation of the results, and transmission to the patient. This succession of operations makes these heavy methods of execution, with long response times, hence major drawbacks for therapeutic and / or prophylactic management.
Or la recrudescence actuelle de ces infections pousse au contraire a rechercher une forte participation au moins a un diagnostic rapide, d'une mise en oeuvre facile et directement par la patiente, dont la rapidité et la fiabilité seraient de nature a supprimer les inconvénients mentionnes ci-dessus et a rendre ce diagnostic d'une utilisation simple et courante, sans perturber le cours habituel des activités de la femme.However the current resurgence of these infections pushes on the contrary to seek a strong participation at least in a rapid diagnosis, of an easy and direct implementation by the patient, whose speed and reliability would be likely to eliminate the drawbacks mentioned above above and to make this diagnosis of a simple and current use, without disturbing the usual course of the activities of the woman.
On connaît divers produits, dispositifs et méthodes destines a prélever et analyser les sécrétions corporelles, en particulier des voies urogenitales basses de la femme, en vue notamment de la détection d'infections d'origine bactérienne, mais pouvant être utilises aussi en vue de tout autre type d'analyse, ou détection de divers éléments chimiques ou biologiques susceptibles de se trouver dans les fluides secrètes.Various products, devices and methods are known for collecting and analyzing bodily secretions, in particular from the lower urogenital tract of women, with a view in particular to the detection of infections of bacterial origin, but which can also be used for any purpose. another type of analysis, or detection of various chemical or biological elements likely to be found in secret fluids.
On connaît par exemple divers types de sondes destines a effectuer des prélèvements de fluides urogenitaux. Ces sondes servent généralement a effectuer uniquement le prélèvement, éventuellement le transfert du fluide a analyser vers le ou les milieux d'analyse, en gênerai contenus dans des flacons, tubes a essais ou similaires, comme le décrit le document EP237394. Un inconvénient de ces procèdes est que la substance a analyser peut être contaminée, ou subir des modifications physico-chimiques ou bactériologiques par les agents de conservation pendant la durée pre-analytique, faussant ainsi l'analyse finale. De plus ce type de prélèvement nécessite un acte gynécologique qui peut être désagréable pour la patiente, et a tout le moins contraignant par rapport a son activité courante.Various types of probes are known, for example, intended for taking samples of urogenital fluids. These probes are generally used to carry out only the sampling, possibly the transfer of the fluid to be analyzed to the analysis medium or media, generally contained in flasks, test tubes or the like, as described in document EP237394. A disadvantage of these procedures is that the substance to be analyzed can be contaminated, or undergo physicochemical or bacteriological modifications by the preservatives during the pre-analytical period, thus distorting the final analysis. In addition, this type of sample requires a gynecological procedure which can be unpleasant for the patient, and at the very least restrictive in relation to her current activity.
En ce qui concerne les analyses d'urine, tous les procèdes classiquement utilises nécessitent de prélever et recueillir l'urine dans un récipient adéquat, puis de transférer celui-ci au lieu d'analyse, moyennant les précautions requises pour assurer la conservation du prélèvement .With regard to urinalysis, all the procedures conventionally used require collecting and collecting urine in a suitable container, then transfer it to the place of analysis, subject to the precautions required to ensure the preservation of the sample.
Pour permettre à la patiente de recueillir elle- même les sécrétions, il a aussi été proposé, notamment dans le document WO 9749338, une serviette hygiénique de conception adaptée à un tel prélèvement, intégrant une bande d'échantillonnage ou un filtre de prélèvement. Ce dispositif est destiné à faciliter la prise d'échantillon, mais ne résout pas les problèmes de délais et d'erreurs d'analyse, car il ne sert en fait qu'à collecter les sécrétions, et à en faciliter le transfert jusqu'au lieu d'analyse. Mais on ne peut éviter les multiples influences des conditions du prélèvement sur la fiabilité et l'exactitude des résultats, et en particulier subsiste l'influence du délai entre prélèvement et analyse sur la viabilité des microorganismes ou 1 ' alcalinisation des urines.To allow the patient to collect secretions herself, it has also been proposed, in particular in document WO 9749338, a sanitary napkin of design adapted to such a sampling, integrating a sampling strip or a sampling filter. This device is intended to facilitate the taking of samples, but does not solve the problems of delays and errors of analysis, because it in fact only serves to collect secretions, and to facilitate their transfer to place of analysis. However, the multiple influences of sampling conditions on the reliability and accuracy of the results cannot be avoided, and in particular the influence of the time between sampling and analysis remains on the viability of microorganisms or the alkalization of urine.
De manière générale, que ce soit pour l'analyse d'urine ou des sécrétions vaginales ou anales, l'opération de prélèvement pose déjà à elle seule de nombreux problèmes contraignants sur la méthodologie et les moyens à utiliser, auxquels s'ajoutent les problèmes des moyens de transport et de conservation, de 1 ' influence des conservateurs sur les méthodes et les résultats de l'analyse, des délais avant et pour l'analyse, couramment effectuée en laboratoire, et des délais de restitution des résultats au patient ou à son médecin, etc. Ces méthodes exigent un équipement et un personnel qualifié, présentent des temps de réponse assez longs, et ont un coût élevé. De plus, les modes de prélèvement habituels peuvent être à l'origine de variations analytiques considérables, pouvant remettre en cause l'exactitude et la fiabilité des résultats des tests. La présente invention a pour but de fournir une solution aux problèmes évoques ci-dessus et vise en particulier a fournir un moyen de détection rapide des infections urmaires et génitales, qui soit d'un usage confortable et permette un diagnostique précoce, rapide et le plus fiable possible par l'utilisatrice elle-même, tout en restant très facile d'utilisation, et d'un coût assez faible.In general, whether for the analysis of urine or vaginal or anal secretions, the sampling operation alone already poses many binding problems on the methodology and the means to be used, to which are added the problems means of transport and storage, the influence of preservatives on the methods and results of the analysis, time before and for analysis, commonly performed in the laboratory, and time to return the results to the patient or to his doctor, etc. These methods require equipment and trained personnel, have fairly long response times, and are expensive. In addition, the usual sampling methods can cause considerable analytical variations, which could call into question the accuracy and reliability of the test results. The present invention aims to provide a solution to the problems mentioned above and aims in particular to provide a means of rapid detection of urinary tract and genital infections, which is comfortable to use and allows early, rapid and most rapid diagnosis. reliable possible by the user herself, while remaining very easy to use, and of a fairly low cost.
L'invention vise a permettre des analyses directes et rapides des paramètres chimiques et germes infectieux a partir des fluides corporels prélevés a des sites précis de la région urogenitale de la femme. Ces analyses directes concernent la détection des indices chimiques d'infection, le dénombrement et l'identification des micro-organismes pathogènes.The invention aims to allow direct and rapid analyzes of chemical parameters and infectious germs from bodily fluids taken from specific sites in the female urogenital region. These direct analyzes relate to the detection of chemical indices of infection, the enumeration and the identification of pathogenic microorganisms.
L'invention a aussi pour objectif de proposer un produit permettant d'assurer un suivi aise d'une cure d'amaigrissement, ou un contrôle de l'efficacité de produits amincissants, par un dosage des corps cetoniques présents dans les fluides recueillis. D'autres objectifs encore sont de permettre un suivi des antibiotherapies, ou fournir une assistance médicale a la procréation ou le suivi de l' evolutivite de la grossesse par un dosage des hormones steroidiennes telles que la progestérone et l'hormone gonadotrophique choπonique (hCG) .The invention also aims to provide a product for ensuring easy monitoring of a weight loss treatment, or a control of the effectiveness of slimming products, by assaying the ketone bodies present in the fluids collected. Still other objectives are to enable monitoring of antibiotic therapy, or to provide medical assistance for procreation or monitoring the progress of pregnancy by assaying steroid hormones such as progesterone and gonadotrophic hormone choπonique (hCG). .
Avec ces objectifs en vue, l'invention a pour objet un dispositif de recueil et d'analyse des sécrétions corporelles de la région urogenitale, comportant un support absorbant adapte pour être mis, par une première face, en contact intime avec le corps dans la région urogenitale et comprenant des moyens pour prélever des fluides corporels en vue de leur analyse, caractérise en ce qu'il comporte, intègre dans le volume du support absorbant, des moyens d'analyse in-situ des dits fluides et des moyens de visualisation pour visualiser les résultats de l'analyse a sa surface.With these objectives in view, the invention relates to a device for collecting and analyzing bodily secretions from the urogenital region, comprising an absorbent support adapted to be brought, by a first face, into intimate contact with the body in the urogenital region and comprising means for withdrawing bodily fluids for their analysis, characterized in that it comprises, integrates into the volume of the absorbent support, means for in-situ analysis of said fluids and display means for viewing the results of the analysis on its surface.
Preferentiellement , la visualisation se fera sur la face opposée a la dite première face. L'article absorbant selon l'invention permet, grâce a des moyens d'analyse adaptes, la détection et le diagnostic direct des paramètres chimiques et/ou des micro-organismes responsables des infections urogenitales basses. Il se présente preferentiellement sous la forme d'une protection féminine telle que serviette hygiénique, protege-slip, tampon, ou encore sous la forme de couche pour enfants ou couche pour incontinence.Preferably, the visualization will be done on the face opposite to said first face. The absorbent article according to the invention allows, thanks to suitable analysis means, the detection and direct diagnosis of the chemical parameters and / or of the microorganisms responsible for low urogenital infections. It preferably comes in the form of feminine protection such as a sanitary napkin, panty liner, tampon, or even in the form of a diaper for children or a layer for incontinence.
Dans sa forme préférentielle, il est constitue de la manière suivante, en couches sensiblement superposées et imbriquées:In its preferred form, it is constituted in the following manner, in substantially superimposed and nested layers:
- une couche absorbante supérieure de contact avec la région urogenitale, comprenant au moins un site de prélèvement des fluides corporels ou échantillons a analyser , - des milieux reactionnels chimiques ou des milieux de culture, constituants les dits moyens d'analyse, des couches et des conduits absorbants et diffusants pour amener les fluides corporels recueillis aux sites de prélèvement vers les dits milieux reactionnels chimiques ou milieux de culture, puis vers une zone de stockage du trop-plein, une pellicule externe imperméable et au moins localement transparente permettant une lecture visuelle des résultats sur les moyens de visualisation. Chaque milieu de culture ou zone reactive contient un chromogene et/ou indicateur colore qui détecte les modifications du milieu provoquées par le métabolisme des micro-organismes qui signe un indice d'infection ou une identification de l'agent pathogène. Dans le cas de détection d'infections des voies urogenitales et anales, le dispositif comportera classiquement trois sites de prélèvement, pour effectuer des prélèvements respectivement d'urine, des sécrétions vaginales, et des sécrétions anales. Dans d'autres applications, un seul ou deux sites de prélèvement pourront suffire.- an upper absorbent layer of contact with the urogenital region, comprising at least one site for sampling bodily fluids or samples to be analyzed, - chemical reaction media or culture media, constituting the said means of analysis, layers and absorbent and diffusing conduits for bringing the bodily fluids collected to the sampling sites to the so-called chemical reaction media or culture media, then to an overflow storage area, an external waterproof and at least locally transparent film allowing a visual reading of the results on the visualization means. Each culture medium or reactive zone contains a chromogen and / or colored indicator which detects the modifications of the medium caused by the metabolism of microorganisms which signals an index of infection or an identification of the pathogenic agent. In the case of detection of urogenital and anal tract infections, the device will include conventionally three sampling sites, for taking samples of urine, vaginal secretions and anal secretions respectively. In other applications, one or two sampling sites may suffice.
Préferentiellement , à chaque site de prélèvement correspond une zone de prélèvement spécifique sur la surface de laquelle sont réparties des cupules d'analyse contenant les moyens d'analyse, en contact direct avec la zone de prélèvement, le fond des cupules affleurant avec la pellicule externe.Preferably, each sampling site corresponds to a specific sampling zone on the surface of which are distributed analysis cups containing the analysis means, in direct contact with the sampling zone, the bottom of the cups flush with the external film. .
Egalement préferentiellement , des canaux de répartition s'étendent à la surface de la zone de prélèvement, entre les cupules d'analyse. Selon d'autres dispositions : la zone de prélèvement communique par sa périphérie avec des conduits d'évacuation de trop-plein qui sont eux même en communication avec la zone de stockage de trop-plein qui est située entre les cupules d'analyse et la pellicule externe et isolée de la zone de prélèvement ;Also preferably, distribution channels extend over the surface of the sampling zone, between the analysis wells. According to other provisions: the sampling zone communicates by its periphery with overflow discharge conduits which are themselves in communication with the overflow storage zone which is located between the analysis cups and the external film and isolated from the sampling area;
- chaque zone de prélèvement comporte à sa surface un film imperméable dans lequel est ménagée une ouverture au niveau du site de prélèvement, et une bande absorbante de récupération recouvre l'ensemble des films imperméables et est en communication par sa périphérie avec la zone de stockage de trop-plein;each sampling zone has on its surface an impermeable film in which an opening is provided at the sampling site, and an absorbent recovery band covers all of the impermeable films and is in communication by its periphery with the storage zone overflow;
- entre les cupules d'analyse et dans la zone de stockage de trop-plein, sont logées des cupules de maintien pour éviter la déformation des cupules d ' analyse .- between the analysis cups and in the overflow storage area, are housed holding cups to avoid deformation of the analysis cups.
Le principe de la serviette de protection féminine selon l'invention repose sur le fait qu'elle absorbe les liquides physiologiques et les diffuse sur toute la surface comportant les zones réactives correspondant a un site de prélèvement concerné. On pourra bien sûr définir sur la serviette plusieurs zones de prélèvement indépendantes, correspondant chacune à un site de prélèvement. Les fluides captés par un site de prélèvement pourront se diffuser sur la surface de la zone de prélèvement correspondante et atteindre les différentes zones réactives de cette zone de prélèvement, mais ne pourront pas diffuser d'une zone de prélèvement à l'autre, pour éviter des mélanges de fluides. Par contre, les trop-pleins de chaque zone de prélèvement seront canalisés vers la zone de stockage de trop plein, isolée et étanche par rapport aux zones de prélèvement et aux zones réactives, où les fluides pourront se mélanger sans gêne, et qui assurera donc une capacité d'absorption maximale . Comme par ailleurs les zones réactives sont visibles par transparence sur la face externe, du côté du slip, de la protection féminine, une simple observation va permettre de voir les changements de coloration de chacune des zones réactives qui auront réagi suite à la présence d'un agent pathogène correspondant au réactif des zones concernées. Il sera ainsi aisé, par une simple observation ou lecture comparative des changements de coloration, de déterminer la présence de tel ou tel agent pathogène en fonction de la coloration de la zone réactive correspondante, et d'établir ainsi un diagnostic d'une infection des voies urogénitales basses.The principle of the feminine protective towel according to the invention is based on the fact that it absorbs physiological liquids and diffuses them over the entire surface comprising the reactive zones corresponding to a sampling site concerned. We can of course define on the towel several independent sampling zones, each corresponding to a sampling site. The fluids captured by a sampling site will be able to diffuse on the surface of the corresponding sampling zone and reach the different reactive zones of this sampling zone, but will not be able to diffuse from one sampling zone to another, to avoid mixtures of fluids. On the other hand, the overflows of each sampling zone will be channeled towards the overflow storage zone, insulated and sealed from the sampling zones and the reactive zones, where the fluids can mix without discomfort, and which will therefore ensure maximum absorption capacity. As the reactive zones are also visible by transparency on the external face, on the side of the panties, of the female protection, a simple observation will make it possible to see the changes in coloration of each of the reactive zones which will have reacted following the presence of a pathogen corresponding to the reagent in the areas concerned. It will thus be easy, by a simple observation or comparative reading of the changes in coloring, to determine the presence of such or such pathogenic agent as a function of the coloring of the corresponding reactive zone, and thus to establish a diagnosis of an infection of the lower urogenital tract.
Un avantage de la protection féminine de type serviette hygiénique est qu'elle constitue un support quasi idéal, dont la mise en oeuvre est particulièrement facile. Placée et maintenue en contact direct avec le corps pendant la période nécessaire à l'analyse, elle permet d'absorber directement les fluides physiologiques et de les amener au contact des réactifs sans délais et également le plus directement possible, en limitant au maximum les risques de contamination exogène des fluides soumis à analyse. De plus elle permet de répondre aux caractéristiques classiques exigées des protections féminines, a savoir une absorption maximale des fluides corporels, obtenue grâce aux zones de canalisation et d'accumulation du trop-plein, qui évacuent dans l'épaisseur de la serviette, comme pour une serviette hygiénique classique, les volumes de fluides excédant ce qui est nécessaire pour alimenter les zones reactives.An advantage of feminine protection of the sanitary napkin type is that it constitutes an almost ideal support, the implementation of which is particularly easy. Placed and maintained in direct contact with the body for the period necessary for the analysis, it makes it possible to directly absorb physiological fluids and bring them into contact with the reagents without delay and also as directly as possible, minimizing the risks of exogenous contamination of fluids subjected to analysis. In addition it allows to respond to classic characteristics required of female protections, namely maximum absorption of body fluids, obtained thanks to the channeling and accumulation areas of the overflow, which evacuate in the thickness of the towel, as for a conventional sanitary napkin, the volumes of fluids exceeding what is necessary to supply the reactive zones.
De plus encore, les dimensions classiques des protections féminines permettent d'y placer assez facilement les éléments reactifs, même en nombre, et de fournir une surface sur laquelle la visualisation des résultats peut être aisément effectuée et observée, donnant une grande facilite de diagnostic sans qu'il soit nécessaire de réaliser de prélèvements spécifiques. II est ici rappelé que les examens uπnaires font appel en premier lieu aux techniques chimiques de détection, compte tenu de leur rapidité et de la nature de l'urine qui est exempte de micro-organismes a l'état normal, ces techniques constituant un bon support de diagnostic d'orientation. Par contre, les examens des sécrétions urogénitales sont plus difficiles a effectuer en raison de la présence d'une flore commensale coexistante avec la flore purement pathogène. Pour cela, on fait preferentiellement appel aux techniques biochimiques sur milieux sélectifs de culture, qui permettent d'identifier les agents infectieux a l'origine de la pathologie.Furthermore, the conventional dimensions of the female protections make it possible to place the reactive elements therein, even in number, fairly easily and to provide a surface on which the visualization of the results can be easily carried out and observed, giving great ease of diagnosis without that it is necessary to make specific samples. It is recalled here that the uπnaires examinations first use chemical detection techniques, taking into account their speed and the nature of the urine which is free from microorganisms in the normal state, these techniques constituting a good orientation diagnostic support. On the other hand, examinations of urogenital secretions are more difficult to perform due to the presence of a commensal flora coexisting with the purely pathogenic flora. For this, use is preferably made of biochemical techniques on selective culture media, which make it possible to identify the infectious agents at the origin of the pathology.
Différentes méthodes d'analyse et de détection pourront donc être utilisées, soit conjointement sur le même produit, présentant alors des sites reactifs appropries a ces différents types de détection, soit sur des produits spécifiques au types de diagnostics envisages, cette solution étant plus particulièrement adaptée dans le cas ou les temps de reaction des différentes méthodes de détections sont sensiblement différents. Par exemple, un modèle du produit pourra être utilisé pour fournir une méthode de détection chimique rapide, en 30 minutes environ, utilisant les indices d'infection urinaires ; et un autre modèle proposera une méthode micro-biologique semi-rapide utilisant des milieux de culture permettant d'identifier l'agent pathogène. Dans les deux cas, la détection des infections urogénitales basses selon l'invention est une méthode rapide, simple et précise, qui permet d'obtenir une réponse dans un temps particulièrement réduit, de moins d'une heure.Different methods of analysis and detection could therefore be used, either jointly on the same product, thus presenting reactive sites suitable for these different types of detection, or on products specific to the types of diagnostics envisaged, this solution being more particularly suitable in the case where the reaction times of the different detection methods are significantly different. For example, a product model could be used to provide a method of rapid chemical detection, in approximately 30 minutes, using clues of urinary tract infection; and another model will propose a semi-rapid micro-biological method using culture media making it possible to identify the pathogenic agent. In both cases, the detection of low urogenital infections according to the invention is a rapid, simple and precise method, which makes it possible to obtain a response in a particularly short time, of less than an hour.
Pour satisfaire aux souhaits d'analyse rapide, le milieu de culture type préférentiel comprend une base nutritive nécessaire au développement des micro¬ organismes, de type connu en soi, un activateur de croissance, un substrat chromogénique ou fluorogénique et un ou des antibiotiques inhibant la croissance des micro¬ organismes indésirés dans le milieu de culture.To meet the rapid analysis wishes, the preferred type of culture medium comprises a nutrient base necessary for the development of micro ¬ organisms, of type known per se, a growth enhancer, a chromogenic or fluorogenic substrate and one or more antibiotics inhibit ¬ unwanted growth of micro organisms in the culture medium.
Les substrats chromogènes ou fluorogènes sont ceux utilisés de manière conventionnelle pour la détection des micro-organismes. De préférence on choisira pour chaque micro-organisme à détecter un substrat présentant une intensité d'hydrolyse liée à une forte coloration visible à l'œil nu, une affinité enzymatique maximale pour l'activité à détecter et une bonne solubilité qui limite le signal au voisinage immédiat des colonies. L'amplification du signal par libération du groupement chromophore ou fluorophore est visualisée sans nécessiter l'emploi d'un appareil tel qu'une lampe UV ou un spectrophotomètre . Une autre variante de la méthode d' identification colorimétrique, bien connue de l'homme de l'art, consiste à introduire dans le milieu de culture des substances qui détectent les faibles changements générés par la croissance des micro-organismes. Ces substances sont choisies parmi les indicateurs de pH ou les indicateurs d' oxydoréduction (redox) . Divers activateurs peuvent être ajoutés au milieu de culture pour potentialiser l'activité enzymatique à détecter. Il peut s'agir des hexosamines, des sels composés cationiques bivalents tels que les sels de Mn2+, Mg2+, Ca2XThe chromogenic or fluorogenic substrates are those conventionally used for the detection of microorganisms. Preferably, for each microorganism to be detected, a substrate having a hydrolysis intensity linked to a strong coloration visible to the naked eye will be chosen, a maximum enzymatic affinity for the activity to be detected and good solubility which limits the signal to the immediate vicinity of the colonies. The amplification of the signal by release of the chromophore or fluorophore group is visualized without requiring the use of a device such as a UV lamp or a spectrophotometer. Another variant of the colorimetric identification method, well known to those skilled in the art, consists in introducing into the culture medium substances which detect the slight changes generated by the growth of microorganisms. These substances are chosen from pH indicators or redox indicators. Various activators can be added to the culture medium to potentiate the enzymatic activity to be detected. They may be hexosamines, bivalent cationic compound salts such as the salts of Mn 2+ , Mg 2+ , Ca 2 X
Des agents de perméabilisation ou tensioactifs peuvent aussi être ajoutés pour favoriser une meilleure liaison enzyme-substrat.Permeabilization agents or surfactants can also be added to promote better enzyme-substrate bonding.
Selon une réalisation préférentielle, le milieu de culture contient au moins un inhibiteur des microorganismes non recherchés. Cet inhibiteur, en présence d'autres constituants du milieu, y compris les composés chromogènes, permet d'obtenir un milieu de culture tout à fait spécifique du genre de micro-organisme recherché. Ainsi, le milieu de culture permet la détection spécifique ainsi que le dénombrement des micro-organismes recherchés .According to a preferred embodiment, the culture medium contains at least one inhibitor of microorganisms not sought. This inhibitor, in the presence of other constituents of the medium, including the chromogenic compounds, makes it possible to obtain a culture medium entirely specific to the kind of microorganism sought. Thus, the culture medium allows specific detection as well as the counting of the microorganisms sought.
D'autres caractéristiques et avantages apparaîtront dans la description qui va être faite d'une serviette hygiénique de prélèvement et d'analyse, conforme à 1 ' invention .Other characteristics and advantages will appear in the description which will be given of a sanitary napkin for sampling and analysis, in accordance with the invention.
On se reportera aux dessins annexés dans lesquels :Reference is made to the appended drawings in which:
- la figure 1 est une vue en plan de la serviette illustrant la disposition des différents éléments qui la constitue, étant bien entendu que ces différents éléments sont intégrés dans l'épaisseur de la serviette et donc non réellement visibles de l'extérieur;- Figure 1 is a plan view of the towel illustrating the arrangement of the different elements which constitute it, it being understood that these different elements are integrated into the thickness of the towel and therefore not really visible from the outside;
- la figure 2 est une vue en coupe transversale, à échelle agrandie, selon la ligne II-II de la figure 1, la figure 3 est une vue partielle en coupe longitudinale selon la ligne III-III de la figure 1,FIG. 2 is a cross-sectional view, on an enlarged scale, along the line II-II of FIG. 1, FIG. 3 is a partial view in longitudinal section along the line III-III of FIG. 1,
- la figure 4 est une vue très schématique en coupe longitudinale illustrant la juxtaposition des trois zones de prélèvement, - la figure 5 est une vue de côte d'une variante de réalisation, destinée a améliorer la précision de la localisation des prélèvements,FIG. 4 is a very schematic view in longitudinal section illustrating the juxtaposition of the three sampling zones, FIG. 5 is a side view of an alternative embodiment, intended to improve the accuracy of the location of the samples,
- les figures 6 a 8 illustrent schematiquement la face de visualisation de la serviette dans différentes variantes .- Figures 6 to 8 schematically illustrate the display face of the towel in different variants.
Sur le dessin de la figure 1, on voit la disposition relative des trois zones de détection 11, 12, 13, correspondant respectivement a la zone de détection des infections uπnaires 11, la zone de détection des infections génitales 12, et la zone de détection des infections anales 13, qui sont également schematiquement représentées sur la figure 4. A part leurs dimensions, la constitution générale de ces trois zones est similaire. Les parties d'extrémités 14, 15 de la serviette n'ont pas de fonction particulière sinon de servir de maintien en position fixe pendant le temps nécessaire au recueil des échantillons, par exemple par adhérence sur le fond du slip. Comme on le voit figure 4, chaque zone comporte un renflement supérieur correspondant, comme on le verra par la suite, aux sites de prélèvement 110, 120, 130. Ces renflements correspondent respectivement a la localisation du méat unnaire pour la zone de détection 11, du canal vaginal pour la zone de détection 12, et a l'anus pour la zone de détection 13. Ils permettent d'assurer un contact optimal avec les parties du corps origines des sécrétions a analyser. Dans la variante représentée figure 5, la forme particulière en ventouse donnée aux sites de prélèvement vise a assurer un contact encore plus étroit et surtout très localise avec les sources des sécrétions, en particulier pour effectuer des prélèvements très localises, et éviter ainsi au maximum des perturbations qui pourraient être provoquées par l'absorption dans les zones de prélèvement de sécrétions contaminantes en provenance de la périphérie des sources de sécrétions à analyser.In the drawing of FIG. 1, we see the relative arrangement of the three detection zones 11, 12, 13, corresponding respectively to the detection zone for uπnary infections 11, the detection zone for genital infections 12, and the detection zone anal infections 13, which are also shown diagrammatically in FIG. 4. Apart from their dimensions, the general constitution of these three zones is similar. The end portions 14, 15 of the towel have no particular function other than serving to hold them in a fixed position for the time necessary for collecting the samples, for example by adhesion to the bottom of the pants. As can be seen in FIG. 4, each zone has an upper bulge corresponding, as will be seen below, to the sampling sites 110, 120, 130. These bulges correspond respectively to the location of the unary meatus for the detection zone 11, from the vaginal canal for the detection zone 12, and to the anus for the detection zone 13. They make it possible to ensure optimal contact with the parts of the body which are the origins of the secretions to be analyzed. In the variant shown in FIG. 5, the particular shape of the suction cup given to the sampling sites aims to ensure even closer and above all very localized contact with the sources of the secretions, in particular for carrying out very localized sampling, and thus avoiding as much as possible disturbances that could be caused by absorption in the secretion collection areas contaminants from the periphery of the secretion sources to be analyzed.
Typiquement, le dispositif de détection 1 comprend une enveloppe constituée, du côté de la face supérieure 2 destinée a être placée au contact du corps, par un voile de contact 21, en matériau doux et poreux, recouvrant toute la surface supérieure, et du côté oppose 3 , vers le bas, d'une pellicule imperméable 22 , par exemple un mince film de matière plastique, résistant à la traction et aux produits chimiques, qui se raccorde au voile de contact en enveloppant la périphérie de la serviette, comme on le voit figure 2, et qui assure l'étanchéite du dispositif vers l'extérieur.Typically, the detection device 1 comprises an envelope constituted, on the side of the upper face 2 intended to be placed in contact with the body, by a contact veil 21, of soft and porous material, covering the entire upper surface, and on the side opposes 3, downwards, an impermeable film 22, for example a thin film of plastic material, resistant to traction and to chemicals, which is connected to the contact veil by enveloping the periphery of the towel, as is see Figure 2, and which seals the device to the outside.
A l' intérieur de cette enveloppe se trouvent vers le haut les éléments servant aux prélèvements, et vers le bas la zone 23 de stockage du trop plein des fluides corporels captés par la serviette.Inside this envelope are located upward the elements used for the samples, and downwards the zone 23 for storing the overflow of bodily fluids captured by the towel.
Immédiatement sous le voile de contact 21 est disposée une bande de récupération des sécrétions contaminantes 24 qui repose sur un film imperméable 25. Des ouvertures 26 sont ménagées dans ce film au niveau des sites de prélèvements pour permettre le passage de fluides à analyser vers les zones de prélèvement qui se trouvent sous le film. Chaque zone de prélèvement est formée d'une couche absorbante 27, par exemple en coton pur (constitue par une succession des motifs de cellulose) ou en une autre matière a base de cellulose, d'acétate de cellulose ou autres dérives, ou encore d'autres matières très absorbantes offrant de bonnes caractéristiques de résistance et porosité, dans des épaisseurs relativement réduites .Immediately under the contact veil 21 is disposed a strip for recovering contaminating secretions 24 which rests on an impermeable film 25. Openings 26 are made in this film at the level of the sampling sites to allow the passage of fluids to be analyzed towards the zones under the film. Each sampling zone is formed of an absorbent layer 27, for example of pure cotton (constituted by a succession of cellulose patterns) or of another material based on cellulose, cellulose acetate or other derivatives, or else d 'other highly absorbent materials offering good resistance and porosity characteristics, in relatively reduced thicknesses.
Chaque couche absorbante 27 repose sur une plaque souple 28 en matériau imperméable, conformée de manière a présenter une pluralité de cupules d'analyses 29 de forme troncomque et qui s'étendent jusqu'à la pellicule étanche 22. Chaque cupule 29 contient un réactif chimique ou un milieu réactionnel spécifiquement destiné à assurer la détection d'un agent infectieux. Le fond de chaque cupule est en contact avec la pellicule étanche et transparente 22, qui permet ainsi de visualiser les changements de coloration dans les cupules provoqués par la réaction du réactif chimique ou du milieu de culture avec les fluides prélevés et dirigés vers les cupules par la couche absorbante 27. La couche absorbante 27 assure par capillarité le transfert des fluides captés au travers de l'ouverture 26 vers les milieux reactionnels chimiques ou milieux de cultures prédisposés dans les cupules. Comme les dimensions des cupules d'analyses 29 par rapport à celles de la plaque 28 sont relativement faibles, le rapport de la surface totale de la couche absorbante 27 sur la surface totale des ouvertures des cupules est élevé, ce qui favorise la pénétration des fluides captés dans les cupules et le remplissage rapide des cupules, même celles qui sont situées le plus loin du site de prélèvement. Ce remplissage peut de plus être favorisé par l'existence d'un vide ménagé à cet effet dans les cupules d'analyse, qui empêche aussi les phénomènes de surcharge ou de dilution des réactifs. Par ailleurs, des canaux de diffusions 30 sont préferentiellement réalisés à la surface de la plaque 28 pour accélérer et homogénéiser la répartition des fluides sur toute la surface de la plaque et donc dans les différentes cupules d'analyse.Each absorbent layer 27 rests on a flexible plate 28 of impermeable material, shaped so as to have a plurality of analysis cups 29 of truncated shape and which extend up to the film. sealed 22. Each well 29 contains a chemical reagent or a reaction medium specifically intended to ensure the detection of an infectious agent. The bottom of each well is in contact with the waterproof and transparent film 22, which thus makes it possible to visualize the changes in color in the wells caused by the reaction of the chemical reagent or of the culture medium with the fluids withdrawn and directed towards the wells. the absorbent layer 27. The absorbent layer 27 ensures by capillarity the transfer of the fluids captured through the opening 26 towards the chemical reaction media or culture media predisposed in the wells. As the dimensions of the analysis wells 29 compared to those of the plate 28 are relatively small, the ratio of the total surface of the absorbent layer 27 to the total surface of the openings of the wells is high, which promotes the penetration of fluids captured in the wells and the rapid filling of the wells, even those located furthest from the sampling site. This filling can also be favored by the existence of a vacuum provided for this purpose in the analysis wells, which also prevents the phenomena of overloading or dilution of the reagents. Furthermore, diffusion channels 30 are preferably made on the surface of the plate 28 to accelerate and homogenize the distribution of the fluids over the entire surface of the plate and therefore in the various analysis wells.
Les cupules d'analyses ont préferentiellement une forme tronconique favorable à un meilleur contact de fluides avec le milieu réactionnel qu'elles contiennent. La chambre réactionnelle formée par le fond des cupules 29 sera préferentiellement conçue de manière à ne pas être totalement pleine et conserver une bulle d'air qui favorise le mélange du milieu réactionnel. L'ajout du fluide corporel est réalisé par aspiration directe dans la chambre réactionnelle qui contient les reactifs nécessaires a la réalisation des reactions de détection des agents infectieux. Ces cupules sont preferentiellement constituées d'un matériau tel que le polypropylene qui assure une bonne diffusion de la chaleur et maintien la température autour de 37 °C dans les cupules, ce qui constitue un micro-incubateur nécessaire a la croissance des micro-organismes et a leur détection. A la périphérie de la plaque 28, des bandes ou conduits absorbants et diffusants 31, 32 sont disposes, en contact avec les bords de la couche absorbante 27, pour rediriger le trop-plein de fluide vers la zone de stockage de trop-plein 23, constituée d'un matériau très absorbant reparti entre la plaque 28 et la pellicule 22, dans les intervalles entre les cupules 29. Les canaux de diffusion 30 s'étendent preferentiellement longitudmalement pour repartir au mieux les fluides, et débouchent dans les conduits de trop-plein 32, comme on le voit figure 1.The analysis wells preferably have a frustoconical shape favorable to better contact of fluids with the reaction medium which they contain. The reaction chamber formed by the bottom of the wells 29 will preferably be designed so as not to be completely full and to retain an air bubble which promotes mixing of the reaction medium. The addition of body fluid is achieved by direct aspiration in the reaction chamber which contains the reagents necessary for carrying out the reactions for detecting infectious agents. These wells are preferably made of a material such as polypropylene which ensures good heat distribution and maintains the temperature around 37 ° C. in the wells, which constitutes a micro-incubator necessary for the growth of microorganisms and upon detection. At the periphery of the plate 28, absorbent and diffusing strips or conduits 31, 32 are arranged, in contact with the edges of the absorbent layer 27, to redirect the fluid overflow to the overflow storage area 23 , consisting of a very absorbent material distributed between the plate 28 and the film 22, in the intervals between the cups 29. The diffusion channels 30 preferably extend longitudinally to distribute the fluids as well as possible, and open into the excess conduits -full 32, as seen in Figure 1.
Des cupules de maintien 33 sont preferentiellement disposées dans la zone de stockage de trop plein, entre les cupules d'analyses, comme on le voit sur les figures 2 et 3. Ces cupules peuvent aussi être réalisées sous la forme d'une plaque preformee comme la plaque 28. Elles sont aussi tronconiques, inversées par rapport aux cupules d'analyse, percées et remplies d'une matière très absorbante. Outre leur participation au captage du trop- plein, elles donnent une certaine rigidité au dispositif en empêchant son écrasement et donc la déformation des cupules d'analyse, ce qui assure le maintien du volume réactionnel initial et donc la justesse et la reproductibilite des résultats des analyses.Holding cups 33 are preferably arranged in the overflow storage zone, between the analysis cups, as seen in FIGS. 2 and 3. These cups can also be produced in the form of a preformed plate as plate 28. They are also tapered, inverted with respect to the analysis cups, pierced and filled with a very absorbent material. In addition to their participation in the collection of the overflow, they give a certain rigidity to the device by preventing its crushing and therefore the deformation of the analysis cups, which ensures the maintenance of the initial reaction volume and therefore the accuracy and reproducibility of the results of the analyzes.
Le film imperméable 25 s'étend sur et autour des zones de prélèvements, pour les border vers l'extérieur et les séparer l'une de l'autre. Il évite ainsi un mélange par diffusion des fluides provenant des sites de prélèvement voisins. Il évite aussi un mélange des fluides contaminants captes par la bande de récupération 24 avec les fluides a analyser captes par les couches absorbantes 27. La bande 24 se prolonge sur les bords de la serviette en formant des canaux de drainage périphériques 34 qui transfèrent les sécrétions contaminantes vers les bandes latérales de trop-plein 31 et la zone de stockage de trop-plein 23. Les figures 6 a 8 montrent a titre d'illustration différentes possibilités, nullement limitatives, de dispositions des moyens de visualisation du côte de la face inférieure, c'est a dire celle sur laquelle les résultats des analyses sont rendus visibles par changement de couleur des reactifs contenus dans les cupules 29, le changement de couleur étant perçu au travers du fond 35 des cupules et de la pellicule externe 22.The impermeable film 25 extends over and around the sampling zones, to border them outwards and separate them from one another. It thus avoids a mixing by diffusion of fluids from neighboring sampling sites. It also avoids mixing of the contaminating fluids captured by the recovery band 24 with the fluids to be analyzed captured by the absorbent layers 27. The band 24 extends along the edges of the napkin by forming peripheral drainage channels 34 which transfer the secretions contaminating towards the side overflow strips 31 and the overflow storage area 23. FIGS. 6 to 8 show by way of illustration various possibilities, in no way limiting, of arrangements of display means on the side of the lower face , that is to say the one on which the results of the analyzes are made visible by change of color of the reagents contained in the wells 29, the change in color being perceived through the bottom 35 of the wells and of the external film 22.
La figure 6 montre par exemple un ensemble de dix zones indicatrices 35 correspondant a dix analyses de type chimique, c'est a dire des tests rapides, par exemple esterase leucocytaire, nitrites, pH, albumine, glucose, corps cetoniques, protéines, hémoglobine, bilirubine, urobilmogene . La figure 7 montre de manière similaire les zones indicatrices 35 dans le cas de tests semi-rapides pour la détection d'agent infectieux, tels que par exemple : Escheπchia coli, Neisseπa gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Levures, Herpès simplex, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa.FIG. 6 shows for example a set of ten indicator zones 35 corresponding to ten chemical type analyzes, that is to say rapid tests, for example leukocyte esterase, nitrites, pH, albumin, glucose, ketone bodies, proteins, hemoglobin, bilirubin, urobilmogene. FIG. 7 similarly shows the indicator zones 35 in the case of semi-rapid tests for the detection of infectious agent, such as for example: Escheπchia coli, Neisseπa gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Yeasts, Herpes simplex, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa.
Enfin, la figure 8 illustre le cas d'un dispositif utilise pour la détection des indices chimiques de minceur, en présentant des indicateurs colores de : acétone, acide aceto-acetique, acide beta- hydroxybutyπque, glucose, creatinme. L'invention n'est pas limitée au mode de réalisation décrit ci-dessus uniquement à titre d'exemple, et la technologie de réalisation pourra être modifiée et adaptée sans sortir du cadre de l'invention telle que définie par les revendications annexées.Finally, FIG. 8 illustrates the case of a device used for the detection of chemical slimming indices, by presenting colored indicators of: acetone, aceto-acetic acid, beta-hydroxybutyπque acid, glucose, creatinme. The invention is not limited to the embodiment described above solely by way of example, and the technology of embodiment can be modified and adapted without departing from the scope of the invention as defined by the appended claims.
Ses applications ne sont pas non plus limitées aux applications préférentielles qui ont déjà été indiquées précédemment. En particulier, l'homme du métier pourra aisément adapter la topologie et la nature des réactifs pour détecter d'autres pathologies ou états physiologiques, par exemple : détection des indices chimiques de minceur, de 1 ' ostéoporose, de la néphropathie, des myopathies, la détection des maladies infectieuses (fièvre typhoïde, rougeole, tuberculose, pneumonies, sarcomes généralisés ou maladie de Hodgkin) , détection des intoxications (plomb, phosphore, mercure, cadmium, uranium, bismuth), les avitaminoses, la détection d'un retard mental, la détection de tumeurs osseuses, 1 ' hyperparathyroïdie, le diagnostic prénatal des malformations congénitales, le diagnostic de grossesse, l'assistance médicale à la procréation, le suivi de l' évolutivité de la grossesse, etc. Its applications are also not limited to the preferential applications which have already been indicated previously. In particular, a person skilled in the art can easily adapt the topology and the nature of the reagents to detect other pathologies or physiological states, for example: detection of chemical indicators of thinness, osteoporosis, nephropathy, myopathies, detection of infectious diseases (typhoid fever, measles, tuberculosis, pneumonia, general sarcomas or Hodgkin's disease), detection of intoxication (lead, phosphorus, mercury, cadmium, uranium, bismuth), avitaminosis, detection of mental retardation , detection of bone tumors, hyperparathyroidism, prenatal diagnosis of congenital malformations, diagnosis of pregnancy, medically assisted procreation, monitoring of the progress of pregnancy, etc.

Claims

REVENDICATIONS
1. Dispositif de recueil et d'analyse des sécrétions corporelles de la région urogenitale, comportant un support absorbant adapté pour être mis, par une première face (2), en contact intime avec le corps dans la région urogenitale et comprenant des moyens pour prélever des fluides corporels en vue de leur analyse, caractérisé en ce qu'il comporte, intégre dans le volume du support absorbant (1), des moyens (29) d'analyse in- situ des dits fluides et des moyens de visualisation (35) pour visualiser les résultats de l'analyse à sa surface (3) .1. Device for collecting and analyzing bodily secretions from the urogenital region, comprising an absorbent support adapted to be brought, by a first face (2), into intimate contact with the body in the urogenital region and comprising means for removing body fluids for their analysis, characterized in that it includes, in the volume of the absorbent support (1), means (29) for in situ analysis of said fluids and display means (35) to view the results of the analysis on its surface (3).
2. Dispositif selon la revendication 1, caractérise en ce que les moyens de visualisation (35) sont situés sur la face (3) opposée à la première face (2) .2. Device according to claim 1, characterized in that the display means (35) are located on the face (3) opposite the first face (2).
3. Dispositif selon la revendication 2, caractérisé en ce qu'il comporte, en couches sensiblement superposées et imbriquées: - une couche absorbante supérieure (21) de contact avec la région urogenitale, comprenant au moins un site (110, 120, 130) de prélèvement des fluides corporels a analyser,3. Device according to claim 2, characterized in that it comprises, in substantially superimposed and nested layers: - an upper absorbent layer (21) of contact with the urogenital region, comprising at least one site (110, 120, 130) of bodily fluids to be analyzed,
- des milieux reactionnels chimiques ou des milieux de culture, constituants les dits moyens d'analyse (29),- chemical reaction media or culture media, constituting said means of analysis (29),
- des couches et des conduits (27, 31) absorbants et diffusants pour amener les fluides corporels recueillis aux sites de prélèvement vers les dits milieux reactionnels chimiques ou milieux de culture, puis vers une zone de stockage du trop-plein (23), une pellicule externe (22) imperméable et au moins localement transparente permettant une lecture visuelle des résultats sur les moyens de visualisation (35) . - absorbent and diffusing layers and conduits (27, 31) for bringing the bodily fluids collected to the sampling sites to the so-called chemical reaction media or culture media, then to an overflow storage area (23), a waterproof and at least locally transparent external film (22) allowing visual reading of the results on the display means (35).
4. Dispositif selon la revendication 3, caractérise en ce qu'il comporte trois sites de prélèvements (110, 120, 130), pour effectuer des prélèvements respectivement d'urine, des sécrétions vaginales et des sécrétions anales.4. Device according to claim 3, characterized in that it comprises three sampling sites (110, 120, 130), for taking samples of urine, vaginal secretions and anal secretions respectively.
5. Dispositif selon la revendication 3, caractérise en ce qu'à chaque site de prélèvement correspond une zone de prélèvement spécifique (11, 12, 13) sur la surface de laquelle sont reparties des cupules d'analyse (29) contenant les moyens d'analyse, en contact direct avec la zone de prélèvement, le fond (35) des cupules affleurant avec la pellicule externe (22) .5. Device according to claim 3, characterized in that each sampling site corresponds to a specific sampling zone (11, 12, 13) on the surface of which are distributed analysis cups (29) containing the means of analysis, in direct contact with the sampling zone, the bottom (35) of the cups flush with the external film (22).
6. Dispositif selon la revendication 5, caractérise en ce que des canaux de répartition (30) s'étendent à la surface de la zone de prélèvement (27), entre les cupules d ' analyse .6. Device according to claim 5, characterized in that distribution channels (30) extend over the surface of the sampling zone (27), between the analysis cups.
7. Dispositif selon la revendication 5, caractérise en ce que la zone de prélèvement (27) communique par sa périphérie avec des conduits (31, 32) d'évacuation de trop-plein qui sont eux même en communication avec la zone de stockage de trop-plein (23) qui est située entre les cupules d'analyse (29) et la pellicule externe (22) et isolée de la zone de prélèvement (27) .7. Device according to claim 5, characterized in that the withdrawal zone (27) communicates by its periphery with conduits (31, 32) for overflow discharge which are themselves in communication with the storage zone of overflow (23) which is located between the analysis cups (29) and the external film (22) and isolated from the sampling zone (27).
8. Dispositif selon la revendication 5, caractérise en ce que chaque zone de prélèvement (27) comporte a sa surface un film imperméable (25) dans lequel est ménagée une ouverture (26) au niveau du site de prélèvement, et une bande absorbante (24) de récupération recouvre l'ensemble des films imperméables et est en communication par sa périphérie (34) avec la zone de stockage de trop- plein (23) . 8. Device according to claim 5, characterized in that each sampling zone (27) has on its surface an impermeable film (25) in which an opening (26) is formed at the sampling site, and an absorbent strip ( 24) of recovery covers all of the waterproof films and is in communication via its periphery (34) with the overflow storage area (23).
9. Dispositif selon la revendication 5, caractérise en ce qu'il comporte, entre les cupules d'analyse (29) et dans la zone de stockage de trop-plein (23), des cupules de maintien (33) pour éviter la déformation des cupules d'analyse.9. Device according to claim 5, characterized in that it comprises, between the analysis cups (29) and in the overflow storage area (23), holding cups (33) to prevent deformation analysis wells.
10. Dispositif selon la revendication 1, caractérise en ce qu' il comporte des moyens d' analyse pour détecter un ou plusieurs des agents infectieux suivants : Candida albicans, Chlamydia trachomatis, Corynebacteπum vaginalis, Enterobacteries, Enterococcus , Escheπchia coli, Gardnerella vaginalis, Haemophilus vaginalis, Klebsiella, Mycoplasma hominis, Neisseπa gonorrhoeae, Trichomonas vaginalis, gonocoque, Levures, Herpès simplex, Pseudomonas aerugmosa, Proteus mirabilis, Staphylococcus aureus, Streptocoque beta- hemolytique, Ureaplasma urealyticum. 10. Device according to claim 1, characterized in that it comprises analysis means for detecting one or more of the following infectious agents: Candida albicans, Chlamydia trachomatis, Corynebacteπum vaginalis, Enterobacteries, Enterococcus, Escheπchia coli, Gardnerella vaginalis, Haemophilus vaginalis, Klebsiella, Mycoplasma hominis, Neisseπa gonorrhoeae, Trichomonas vaginalis, gonococcus, Yeasts, Herpes simplex, Pseudomonas aerugmosa, Proteus mirabilis, Staphylococcus aureus, Streptococcus beta-hemolytic, Ureaplasma urealyticum.
PCT/FR2000/001213 1999-05-19 2000-05-05 Device for the collection and analysis of body secretions WO2000072009A1 (en)

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FR9906441A FR2793883A1 (en) 1999-05-19 1999-05-19 Device for analyzing body secretions from the urogenital region, e.g. for diagnosis of lower urogenital infections, comprising absorbent support including systems for in situ analysis and visualization of results
FR99/06441 1999-05-19

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006042871A1 (en) * 2004-09-14 2006-04-27 Sator & Fata, S.L. Compress for female use
CN114989961A (en) * 2022-08-08 2022-09-02 宁波久瑞生物工程有限公司 Detection device and detection method for detecting vaginal secretion

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024557A1 (en) * 1993-04-16 1994-10-27 The United States Of America, As Represented By Th Article and method for detecting the presence of pathogens in excreta

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024557A1 (en) * 1993-04-16 1994-10-27 The United States Of America, As Represented By Th Article and method for detecting the presence of pathogens in excreta

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006042871A1 (en) * 2004-09-14 2006-04-27 Sator & Fata, S.L. Compress for female use
CN114989961A (en) * 2022-08-08 2022-09-02 宁波久瑞生物工程有限公司 Detection device and detection method for detecting vaginal secretion

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FR2793883A1 (en) 2000-11-24

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