WO2000067894A3 - Methods of software driven flow sorting for reiterative synthesis cycles - Google Patents

Methods of software driven flow sorting for reiterative synthesis cycles Download PDF

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Publication number
WO2000067894A3
WO2000067894A3 PCT/US2000/012825 US0012825W WO0067894A3 WO 2000067894 A3 WO2000067894 A3 WO 2000067894A3 US 0012825 W US0012825 W US 0012825W WO 0067894 A3 WO0067894 A3 WO 0067894A3
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WO
WIPO (PCT)
Prior art keywords
groups
microspheres
particles
methods
oligomer
Prior art date
Application number
PCT/US2000/012825
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French (fr)
Other versions
WO2000067894A9 (en
WO2000067894A2 (en
Inventor
Michael Stewart
Alaganadan Nanthakumar
Andrew Watson
Original Assignee
Axys Pharm Inc
Michael Stewart
Alaganadan Nanthakumar
Andrew Watson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axys Pharm Inc, Michael Stewart, Alaganadan Nanthakumar, Andrew Watson filed Critical Axys Pharm Inc
Priority to AU47108/00A priority Critical patent/AU4710800A/en
Publication of WO2000067894A2 publication Critical patent/WO2000067894A2/en
Publication of WO2000067894A3 publication Critical patent/WO2000067894A3/en
Publication of WO2000067894A9 publication Critical patent/WO2000067894A9/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00592—Split-and-pool, mix-and-divide processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00695—Synthesis control routines, e.g. using computer programs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00698—Measurement and control of process parameters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Methods are provided for synthesizing libraries of complex organic molecules on labeled particles. A set of particles encoded with varying levels and combinations of dyes, which provide a detectable address, are used as the support for organic synthesis. The addresses of a set of particles is read by flow cytometry, and used to classify the microspheres. The set of microspheres is then sorted into groups by flow cytometry, using a modified look up table. Monomers are coupled to each microsphere in a group, where each group corresponds to a different coupling reaction. The groups are then combined and resorted, and a second round of addition reactions performed. The reiterative process of sorting into groups; and coupling additional monomers to the growing oligomer chain is performed for sufficient rounds to provide an oligomer of the desired length. The resulting 'liquid array' is a set of encoded microspheres comprising a library of synthesized oligomers, where each sequence in the oligomer library corresponds to a distinct address of fluorescent output data.
PCT/US2000/012825 1999-05-12 2000-05-10 Methods of software driven flow sorting for reiterative synthesis cycles WO2000067894A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU47108/00A AU4710800A (en) 1999-05-12 2000-05-10 Methods of software driven flow sorting for reiterative synthesis cycles

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13402899P 1999-05-12 1999-05-12
US60/134,028 1999-05-12

Publications (3)

Publication Number Publication Date
WO2000067894A2 WO2000067894A2 (en) 2000-11-16
WO2000067894A3 true WO2000067894A3 (en) 2001-07-26
WO2000067894A9 WO2000067894A9 (en) 2001-08-23

Family

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Family Applications (1)

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PCT/US2000/012825 WO2000067894A2 (en) 1999-05-12 2000-05-10 Methods of software driven flow sorting for reiterative synthesis cycles

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AU (1) AU4710800A (en)
WO (1) WO2000067894A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1448789A4 (en) * 2001-10-30 2007-01-03 Nanomics Biosystems Pty Ltd Device and methods for directed synthesis of chemical libraries
US7282355B2 (en) 2002-03-13 2007-10-16 Syngenta Participations Ag Nucleic acid detection method
US20040058333A1 (en) * 2002-09-23 2004-03-25 Bell Michael L. Assay products and procedures
US7326573B2 (en) * 2003-01-10 2008-02-05 Beckman Coulter, Inc. Assay procedures and apparatus
CN101278294B (en) 2005-08-02 2013-04-10 卢米尼克斯股份有限公司 Methods, data structures, and systems for classifying microparticles
CN103911144B (en) * 2014-02-24 2015-11-18 苏州大学 High molecular fluorescent coding microball of europium doped ion and preparation method thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605805A (en) * 1993-02-09 1997-02-25 Becton, Dickinson And Company Automatic lineage assignment of acute leukemias by flow cytometry
WO1997014028A2 (en) * 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5739000A (en) * 1991-08-28 1998-04-14 Becton Dickinson And Company Algorithmic engine for automated N-dimensional subset analysis
US5786219A (en) * 1996-10-28 1998-07-28 Molecular Probes, Inc. Microspheres with fluorescent spherical zones
WO1998053093A1 (en) * 1997-05-23 1998-11-26 Bioarray Solutions Llc Color-encoding and in-situ interrogation of matrix-coupled chemical compounds
WO1999024458A1 (en) * 1997-11-12 1999-05-20 The University Of Queensland Carrier-reporter bead assemblies

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5739000A (en) * 1991-08-28 1998-04-14 Becton Dickinson And Company Algorithmic engine for automated N-dimensional subset analysis
US5605805A (en) * 1993-02-09 1997-02-25 Becton, Dickinson And Company Automatic lineage assignment of acute leukemias by flow cytometry
WO1997014028A2 (en) * 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5786219A (en) * 1996-10-28 1998-07-28 Molecular Probes, Inc. Microspheres with fluorescent spherical zones
WO1998053093A1 (en) * 1997-05-23 1998-11-26 Bioarray Solutions Llc Color-encoding and in-situ interrogation of matrix-coupled chemical compounds
WO1999024458A1 (en) * 1997-11-12 1999-05-20 The University Of Queensland Carrier-reporter bead assemblies

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
A.NANTHAKUMAR ET AL.: "Solid-phase oligonucleotide synthesis and flow cytometric Analysis with Microspheres Encoded with Covalently Attached Fluorophores.", BIOCONJUGATE CHEMISTRY, vol. 11, 2000, pages 282 - 288, XP002159465 *
CLAPHAM B ET AL: "Synthesis and evaluation of a scintillant-containing solid support for use in combinatorial chemistry", TETRAHEDRON LETTERS,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 41, no. 13, March 2000 (2000-03-01), pages 2253 - 2256, XP004192719, ISSN: 0040-4039 *
H.SELIGER ET AL.: "Synthetic Oligonucleotide Combinatorial Libraries - Tools for Studying Nucleid Acid Interactions.", NUCLEOSIDES AND NUCLEOTIDES, vol. 16, no. 5&6, 1997, pages 703 - 710, XP002159464 *
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Publication number Publication date
AU4710800A (en) 2000-11-21
WO2000067894A2 (en) 2000-11-16

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