WO2000066781A3 - Dna balls - Google Patents

Dna balls Download PDF

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Publication number
WO2000066781A3
WO2000066781A3 PCT/US2000/011577 US0011577W WO0066781A3 WO 2000066781 A3 WO2000066781 A3 WO 2000066781A3 US 0011577 W US0011577 W US 0011577W WO 0066781 A3 WO0066781 A3 WO 0066781A3
Authority
WO
WIPO (PCT)
Prior art keywords
ball
hybridization
electron transfer
electron
fet
Prior art date
Application number
PCT/US2000/011577
Other languages
French (fr)
Other versions
WO2000066781A2 (en
Inventor
Akira Ishikawa
Nabuo Takeda
Suzanne I Ahn
Samuel S Ahn
Steven R Hays
Original Assignee
Ball Semiconductor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ball Semiconductor Inc filed Critical Ball Semiconductor Inc
Priority to AU48082/00A priority Critical patent/AU4808200A/en
Publication of WO2000066781A2 publication Critical patent/WO2000066781A2/en
Publication of WO2000066781A3 publication Critical patent/WO2000066781A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0002—Remote monitoring of patients using telemetry, e.g. transmission of vital signals via a communication network
    • A61B5/0031—Implanted circuitry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
    • H01L2924/00—Indexing scheme for arrangements or methods for connecting or disconnecting semiconductor or solid-state bodies as covered by H01L24/00
    • H01L2924/10—Details of semiconductor or other solid state devices to be connected
    • H01L2924/1015—Shape
    • H01L2924/1017—Shape being a sphere

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A rapid gene analysis architecture using a miniature spherical-shaped 'ball' semiconductor device (100). In one aspect, an electron transfer detector (800) is implemented having each molecular probe (802) attached to a gate (810) of a multi-gated FET which is fabricated on the ball (100). An electron acceptor complex (804) is attached near the distal end of a short spacer chain (806) composed of poly(ethylene oxide), linking the molecular probe (802) to the ball (100). Multiple different molecular probes (802) each containing an electron acceptor complex (804) are placed on the ball (100). Target molecules (801) containing electron donor complexes (808) are passed over the ball surface (101) allowing hybridization to occur. Following hybridization, the ball surface (101) is then illuminated causing stimulation of the electron transfer from the donor complex (808) to the acceptor complex (804) located proximally to the gate (810) of the multi-gated FET. The electron transfer reaction is detected by the FET at a site where hybridization has occurred producing an electron transfer reaction. Conversely, if there are probe-target mismatches, the observed electron transfer does not occur. A signal will only be generated for those sites where hybridization is complete. Electron transfer occurs simultaneously at all locations where there was complete hybridization. Hybridization information is then transmitted from the ball (100) to a computer for processing. Hybridization information obtained from analyte-containing fluids passing over single balls or multiple ball addressable arrays in packed columns is transmitted to a computer for analysis and/or process control.
PCT/US2000/011577 1999-04-30 2000-04-28 Dna balls WO2000066781A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU48082/00A AU4808200A (en) 1999-04-30 2000-04-28 Dna balls

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13173299P 1999-04-30 1999-04-30
US60/131,732 1999-04-30

Publications (2)

Publication Number Publication Date
WO2000066781A2 WO2000066781A2 (en) 2000-11-09
WO2000066781A3 true WO2000066781A3 (en) 2002-04-11

Family

ID=22450770

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/011577 WO2000066781A2 (en) 1999-04-30 2000-04-28 Dna balls

Country Status (2)

Country Link
AU (1) AU4808200A (en)
WO (1) WO2000066781A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002311027A (en) * 2001-04-09 2002-10-23 Hitachi Software Eng Co Ltd Beads, manufacturing method of beads, flow cytometer, and program
WO2004053491A1 (en) * 2002-12-09 2004-06-24 Koninklijke Philips Electronics N.V. Biosensor with rf signal transmission
WO2006035392A1 (en) * 2004-09-27 2006-04-06 Koninklijke Philips Electronics N. V. Biosensors for the analysis of samples
WO2006048789A1 (en) 2004-11-05 2006-05-11 Koninklijke Philips Electronics N. V. Detection apparatus and method for use with biosensor emitting rf signals

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992010092A1 (en) * 1990-12-06 1992-06-25 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
WO1997027317A1 (en) * 1996-01-23 1997-07-31 Affymetrix, Inc. Nucleic acid analysis techniques
WO1998025090A1 (en) * 1996-12-04 1998-06-11 Ball Semiconductor Inc. Spherical shaped semiconductor integrated circuit
WO1998053103A1 (en) * 1997-05-21 1998-11-26 Clontech Laboratories, Inc. Nucleic acid arrays
WO2000030534A1 (en) * 1998-11-25 2000-06-02 Ball Semiconductor, Inc. Spherically-shaped biomedical ic

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992010092A1 (en) * 1990-12-06 1992-06-25 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
WO1997027317A1 (en) * 1996-01-23 1997-07-31 Affymetrix, Inc. Nucleic acid analysis techniques
WO1998025090A1 (en) * 1996-12-04 1998-06-11 Ball Semiconductor Inc. Spherical shaped semiconductor integrated circuit
WO1998053103A1 (en) * 1997-05-21 1998-11-26 Clontech Laboratories, Inc. Nucleic acid arrays
WO2000030534A1 (en) * 1998-11-25 2000-06-02 Ball Semiconductor, Inc. Spherically-shaped biomedical ic

Also Published As

Publication number Publication date
AU4808200A (en) 2000-11-17
WO2000066781A2 (en) 2000-11-09

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