WO2000060126A3 - Bioanalyse quantitative d'expression de genes dans des microechantillons - Google Patents

Bioanalyse quantitative d'expression de genes dans des microechantillons Download PDF

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Publication number
WO2000060126A3
WO2000060126A3 PCT/US2000/009526 US0009526W WO0060126A3 WO 2000060126 A3 WO2000060126 A3 WO 2000060126A3 US 0009526 W US0009526 W US 0009526W WO 0060126 A3 WO0060126 A3 WO 0060126A3
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WO
WIPO (PCT)
Prior art keywords
expression
spots
energy
developed
detection
Prior art date
Application number
PCT/US2000/009526
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English (en)
Other versions
WO2000060126A2 (fr
Inventor
Eugenia Wang
Original Assignee
Sir Mortimer B Davis Jewish Ge
Eugenia Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002268695A external-priority patent/CA2268695A1/fr
Priority claimed from US09/299,193 external-priority patent/US6511849B1/en
Application filed by Sir Mortimer B Davis Jewish Ge, Eugenia Wang filed Critical Sir Mortimer B Davis Jewish Ge
Priority to IL14582600A priority Critical patent/IL145826A0/xx
Priority to AU42244/00A priority patent/AU4224400A/en
Priority to JP2000609615A priority patent/JP2004502129A/ja
Priority to EP00921995A priority patent/EP1230383A2/fr
Priority to CA002367413A priority patent/CA2367413A1/fr
Publication of WO2000060126A2 publication Critical patent/WO2000060126A2/fr
Publication of WO2000060126A3 publication Critical patent/WO2000060126A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B01J2219/00707—Processes involving means for analysing and characterising the products separated from the reactor apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne une méthode mise au point pour la détection d'expression ou d'hybridation de gènes dans des microéchantillons, par exemple dans des bibliothèques combinatoires dans lesquelles les quantités sont minimes et les emplacements très rapprochés, pouvant provoquer des situations indésirables dans lesquelles une réaction intense peut déborder sur les emplacements adjacents et, par conséquent, fausser l'exactitude de la réaction des sites voisins. On utilise dans cette bioanalyse un test d'activité enzymatique de digoxigénine pour la détection. L'invention concerne également une méthode mise au point dans le but d'améliorer la fiabilité de l'analyse de l'expression d'ADN dans des microéchantillons, par analyse informatique destinée à normaliser les emplacements. Ce procédé fait appel à des techniques basées sur des modèles adaptables permettant de quantifier automatiquement des données d'échantillons à grande échelle, en dépit d'une possible distorsion spatiale des échantillons. Chaque noeud du modèle adaptable représente l'emplacement d'un gène, et se répète selon la règle de descente de gradient, minimisant une fonction énergie combinant l'énergie de désadaptation des données et l'énergie d'adaptation du modèle.
PCT/US2000/009526 1999-04-08 2000-04-10 Bioanalyse quantitative d'expression de genes dans des microechantillons WO2000060126A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
IL14582600A IL145826A0 (en) 1999-04-08 2000-04-10 Quantitative assay for expression of genes in microarray
AU42244/00A AU4224400A (en) 1999-04-08 2000-04-10 Quantitative assay for expression of genes in microarray
JP2000609615A JP2004502129A (ja) 1999-04-08 2000-04-10 マイクロアレイにおける遺伝子の発現のための定量アッセイ
EP00921995A EP1230383A2 (fr) 1999-04-08 2000-04-10 Bioanalyse quantitative d'expression de genes dans des microechantillons
CA002367413A CA2367413A1 (fr) 1999-04-08 2000-04-10 Bioanalyse quantitative d'expression de genes dans des microechantillons

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
CA2,268,695 1999-04-08
CA002268695A CA2268695A1 (fr) 1999-04-08 1999-04-08 Appareillage et methode d'analyse d'information dans des jeux ordonnes de sites d'information
US12923399P 1999-04-14 1999-04-14
US60/129,233 1999-04-14
US09/299,193 1999-04-23
US09/299,193 US6511849B1 (en) 1999-04-23 1999-04-23 Microarrays of biological materials

Publications (2)

Publication Number Publication Date
WO2000060126A2 WO2000060126A2 (fr) 2000-10-12
WO2000060126A3 true WO2000060126A3 (fr) 2002-06-20

Family

ID=27170957

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/009526 WO2000060126A2 (fr) 1999-04-08 2000-04-10 Bioanalyse quantitative d'expression de genes dans des microechantillons

Country Status (5)

Country Link
EP (1) EP1230383A2 (fr)
JP (1) JP2004502129A (fr)
AU (1) AU4224400A (fr)
IL (1) IL145826A0 (fr)
WO (1) WO2000060126A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001075162A2 (fr) * 2000-03-31 2001-10-11 University Of Louisville Research Foundation, Inc. Jeux ordonnes de microechantillons permettant de cribler des genes regulateurs
US7219016B2 (en) * 2001-04-20 2007-05-15 Yale University Systems and methods for automated analysis of cells and tissues
CN1424405A (zh) * 2001-12-14 2003-06-18 宋克 基因芯片分子探针及相关技术
DE10212958A1 (de) * 2002-03-22 2003-10-09 Friz Biochem Gmbh Verfahren zur Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen
WO2010006727A1 (fr) 2008-07-15 2010-01-21 Institut Pasteur Korea Procédé et appareil pour l'imagerie de caractéristiques sur un substrat
EP2456887B1 (fr) 2009-07-21 2015-11-25 Gen-Probe Incorporated Procédés et compositions pour la détection quantitative de séquences d acides nucléiques sur une gamme dynamique étendue
US20110224088A1 (en) * 2010-03-11 2011-09-15 Heidi Lyng Biomarkers for subtypes of cervical cancer
JP5543888B2 (ja) * 2010-09-29 2014-07-09 富士フイルム株式会社 イムノクロマトグラフ検査方法および装置
JP5543310B2 (ja) * 2010-09-29 2014-07-09 富士フイルム株式会社 イムノクロマトグラフ検査方法および装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0324474A1 (fr) * 1988-01-12 1989-07-19 Roche Diagnostics GmbH Procédé pour détecter des acides nucléiques
WO1998021321A1 (fr) * 1996-11-12 1998-05-22 Qbi Enterprises Ltd. Procede d'identification des genes regules par traduction
WO1998029736A1 (fr) * 1996-12-31 1998-07-09 Genometrix Incorporated Procede et dispositif d'analyse moleculaire multiplexee
WO1998052961A1 (fr) * 1997-05-19 1998-11-26 Mirus Corporation Procede permettant de fixer en une seule etape un marqueur sur des molecules cibles

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0324474A1 (fr) * 1988-01-12 1989-07-19 Roche Diagnostics GmbH Procédé pour détecter des acides nucléiques
WO1998021321A1 (fr) * 1996-11-12 1998-05-22 Qbi Enterprises Ltd. Procede d'identification des genes regules par traduction
WO1998029736A1 (fr) * 1996-12-31 1998-07-09 Genometrix Incorporated Procede et dispositif d'analyse moleculaire multiplexee
WO1998052961A1 (fr) * 1997-05-19 1998-11-26 Mirus Corporation Procede permettant de fixer en une seule etape un marqueur sur des molecules cibles

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHEN JEREMY J W ET AL: "Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 51, no. 3, 1 August 1998 (1998-08-01), pages 313 - 324, XP002159705, ISSN: 0888-7543 *

Also Published As

Publication number Publication date
EP1230383A2 (fr) 2002-08-14
JP2004502129A (ja) 2004-01-22
AU4224400A (en) 2000-10-23
WO2000060126A2 (fr) 2000-10-12
IL145826A0 (en) 2002-07-25

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