WO2000060102A3 - Bacterial fimbrial system for presentation of heterologous peptide sequences - Google Patents

Bacterial fimbrial system for presentation of heterologous peptide sequences Download PDF

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Publication number
WO2000060102A3
WO2000060102A3 PCT/CA2000/000356 CA0000356W WO0060102A3 WO 2000060102 A3 WO2000060102 A3 WO 2000060102A3 CA 0000356 W CA0000356 W CA 0000356W WO 0060102 A3 WO0060102 A3 WO 0060102A3
Authority
WO
WIPO (PCT)
Prior art keywords
agfa
recombinant
salmonella
presentation
expression
Prior art date
Application number
PCT/CA2000/000356
Other languages
French (fr)
Other versions
WO2000060102A2 (en
Inventor
Aaron P White
James L Doran
S Karen Collison
William W Kay
Original Assignee
Innovation And Dev Corp Univer
Aaron P White
James L Doran
S Karen Collison
William W Kay
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innovation And Dev Corp Univer, Aaron P White, James L Doran, S Karen Collison, William W Kay filed Critical Innovation And Dev Corp Univer
Priority to AU36508/00A priority Critical patent/AU3650800A/en
Priority to CA002367210A priority patent/CA2367210A1/en
Publication of WO2000060102A2 publication Critical patent/WO2000060102A2/en
Publication of WO2000060102A3 publication Critical patent/WO2000060102A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a system for creating recombinant agfA fimbrin genes and performing chromosomal gene replacements within Salmonella, creating Salmonella strains which carry the recombinant agfA genes at the native position in the chromosome. One embodiment of the invention is exemplified by the expression of a model epitope (PT3) obtained from the GP63 protein of Leishmania major, by formation of recombinant agfA genes encoding PT3 fusing proteins recombined at 10 different sites throughout the agfA gene. These fusions are shown to be expressed in the thin aggregative fimbriae on the surface of bacterial cell. The AgfA fimbrin of Salmonella (CsgA for E. coli) provides a flexible and stable vehicle for the expression of foreign epitopes in enterobacteriaceae and the subsequent thin aggregative fimbrae (curli) expression product provide an ideal organelle for presentation of the foreign epitopes at the cell surface.
PCT/CA2000/000356 1999-04-05 2000-04-05 Bacterial fimbrial system for presentation of heterologous peptide sequences WO2000060102A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU36508/00A AU3650800A (en) 1999-04-05 2000-04-05 Bacterial fimbrial system for presentation of heterologous peptide sequences
CA002367210A CA2367210A1 (en) 1999-04-05 2000-04-05 Bacterial fimbrial system for presentation of heterologous peptide sequences

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12788899P 1999-04-05 1999-04-05
US60/127,888 1999-04-05

Publications (2)

Publication Number Publication Date
WO2000060102A2 WO2000060102A2 (en) 2000-10-12
WO2000060102A3 true WO2000060102A3 (en) 2001-01-04

Family

ID=22432481

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2000/000356 WO2000060102A2 (en) 1999-04-05 2000-04-05 Bacterial fimbrial system for presentation of heterologous peptide sequences

Country Status (3)

Country Link
AU (1) AU3650800A (en)
CA (1) CA2367210A1 (en)
WO (1) WO2000060102A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120301433A1 (en) * 2009-07-29 2012-11-29 Whitehead Institute For Biomedical Research Bacteriophages expressing amyloid peptides and uses thereof
WO2024050627A1 (en) * 2022-09-07 2024-03-14 Bourgault Steve Csga-derived nanostructures and uses thereof for antigen delivery

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991018092A1 (en) * 1990-05-23 1991-11-28 University Of Maryland At Baltimore Acid-tolerant salmonella typhi, double aro mutants thereof and use as an oral vaccine for typhoid fever

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991018092A1 (en) * 1990-05-23 1991-11-28 University Of Maryland At Baltimore Acid-tolerant salmonella typhi, double aro mutants thereof and use as an oral vaccine for typhoid fever

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
COLLINSON S. K. ET AL.: "Salmonella enteritidis agfBAC operon encoding thin, aggregative fimbriae.", JOURNAL OF BACTERIOLOGY, vol. 178, no. 3, 1996, pages 662 - 667, XP002148884, ISSN: 0021-9193 *
EDWARDS R. A. ET AL.: "Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression", GENE, vol. 207, no. 2, 30 January 1998 (1998-01-30), pages 149 - 157, XP004108913, ISSN: 0378-1119 *
HAMMAR M. ET AL.: "Expression of two csg operons is required for production of fibronectin- and Congo red-binding curli polymers in Escherichia coli K-12.", MOLECULAR MICROBIOLOGY, vol. 18, no. 4, 1995, pages 661 - 670, XP000946742, ISSN: 0950-382X *
HAMMAR M. ET AL.: "Nucleator-dependent intercellular assembly of adhesive curli organelles in Escherichia coli.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 93, no. 13, 1996, 1996, pages 6562 - 6566, XP002148886, ISSN: 0027-8424 *
STENTEBJERG-OLESEN B. ET AL.: "Authentic display of a cholera toxin epitope by chimeric type 1 fimbriae: Effects of insert position and host background.", MICROBIOLOGY, vol. 143, no. 6, 1997, pages 2027 - 2038, XP002148885, ISSN: 1350-0872 *
WHITE A. P. ET AL.: "High efficiency gene replacement in Salmonella enteritidis: chimeric fimbrins containing a T-cell epitope from Leishmania major", VACCINE, vol. 17, no. 17, 23 April 1999 (1999-04-23), pages 2150 - 2161, XP004165000, ISSN: 0264-410X *

Also Published As

Publication number Publication date
CA2367210A1 (en) 2000-10-12
AU3650800A (en) 2000-10-23
WO2000060102A2 (en) 2000-10-12

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