WO2000058503A3 - Improved method for detecting micro-organisms - Google Patents

Improved method for detecting micro-organisms Download PDF

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Publication number
WO2000058503A3
WO2000058503A3 PCT/GB2000/001224 GB0001224W WO0058503A3 WO 2000058503 A3 WO2000058503 A3 WO 2000058503A3 GB 0001224 W GB0001224 W GB 0001224W WO 0058503 A3 WO0058503 A3 WO 0058503A3
Authority
WO
WIPO (PCT)
Prior art keywords
micro
concentration
organisms
fluid
sample
Prior art date
Application number
PCT/GB2000/001224
Other languages
French (fr)
Other versions
WO2000058503A2 (en
Inventor
Kathleen Ann Grant
Stuart Harbron
David Ross Williams
Gordon Chalmers Mellis Young
Original Assignee
Zetatronics Ltd
Kathleen Ann Grant
Stuart Harbron
David Ross Williams
Gordon Chalmers Mellis Young
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9907339A external-priority patent/GB2335981B/en
Priority claimed from GBGB9923442.9A external-priority patent/GB9923442D0/en
Priority claimed from GBGB9925681.0A external-priority patent/GB9925681D0/en
Priority claimed from GB0001238A external-priority patent/GB0001238D0/en
Application filed by Zetatronics Ltd, Kathleen Ann Grant, Stuart Harbron, David Ross Williams, Gordon Chalmers Mellis Young filed Critical Zetatronics Ltd
Priority to AU35681/00A priority Critical patent/AU3568100A/en
Publication of WO2000058503A2 publication Critical patent/WO2000058503A2/en
Publication of WO2000058503A3 publication Critical patent/WO2000058503A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/80—Electrochemical detection via electrodes in contact with culture medium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of identifying one or more micro-organisms in a fluid comprising the steps of: (i) taking a sample of the fluid containing a representative sample of any micro-organism(s) present; (ii) optionally culturing the sample if necessary to increase the number of micro-organisms to a pre-determined range; (iii) suspending aliquots of the fluid containing the micro-organism(s) in a plurality of different solutions, said solutions representing different experimental conditions differing in one or more of the parameters selected from the group of parameters consisting of pH, ionic strength, concentration of surface-active agent, concentration of heavy metal ions, concentration of chelating or complexing agent, concentration of organic solvent, concentration of dye or stain, concentration of polyol, or concentration of polyether; (iv) applying an electric field across a portion of said fluid; (v) measuring the velocity, displacement, zeta potential or electrophoretic mobility of any micro-organisms present following the application of said electric field; (vi) comparing said measured velocities, displacements, zeta potentials or electrophoretic mobilities with tables of velocities, displacements, zeta potentials or electrophoretic mobilities of known micro-organisms measured under substantially identical experimental conditions to determine which, if any, micro-organisms are present.
PCT/GB2000/001224 1999-03-30 2000-03-30 Improved method for detecting micro-organisms WO2000058503A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU35681/00A AU3568100A (en) 1999-03-30 2000-03-30 Improved method for detecting micro-organisms

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
GB9907339A GB2335981B (en) 1998-03-31 1999-03-30 Rapid method for detecting micro-organisms and evaluating antimicrobial activity
GB9907339.7 1999-03-30
GBGB9923442.9A GB9923442D0 (en) 1999-10-05 1999-10-05 Improved method for detecting micro-organisms
GB9923442.9 1999-10-05
GB9925681.0 1999-10-29
GBGB9925681.0A GB9925681D0 (en) 1999-03-30 1999-10-29 Improved method for detection of micro-organisms
GB0001238.5 2000-01-20
GB0001238A GB0001238D0 (en) 1999-03-30 2000-01-20 Improved method for detecting micro-organisms

Publications (2)

Publication Number Publication Date
WO2000058503A2 WO2000058503A2 (en) 2000-10-05
WO2000058503A3 true WO2000058503A3 (en) 2001-02-01

Family

ID=27447754

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/001224 WO2000058503A2 (en) 1999-03-30 2000-03-30 Improved method for detecting micro-organisms

Country Status (3)

Country Link
AU (1) AU3568100A (en)
GB (1) GB2348504B (en)
WO (1) WO2000058503A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0103611D0 (en) * 2001-02-14 2001-03-28 Zetatronics Ltd Further improved method for detecting cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1979000834A1 (en) * 1978-03-24 1979-10-18 High Stoy Tech System for analyzing chemical and biological samples
SU1697004A1 (en) * 1989-09-27 1991-12-07 Всесоюзный научно-исследовательский и испытательный институт медицинской техники Device for determining microorganism motility
WO1995008640A1 (en) * 1993-09-23 1995-03-30 E.I. Du Pont De Nemours And Company An electrophoretic method for the isolation and separation of microorganisms
WO1999060659A1 (en) * 1998-05-15 1999-11-25 Raytheon Company Adaptive antenna pattern control for a multiple access communication system

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2054839B (en) * 1979-06-14 1983-04-27 Secr Defence Determining electrophoretic mobility of cells
GB8926781D0 (en) * 1989-11-27 1990-01-17 Nat Res Dev Identification of micro-organisms
GB2345754B (en) * 1998-03-31 2001-02-07 Zetatronics Ltd Rapid method for detecting micro-organisms and evaluating antimicrobial activity

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1979000834A1 (en) * 1978-03-24 1979-10-18 High Stoy Tech System for analyzing chemical and biological samples
SU1697004A1 (en) * 1989-09-27 1991-12-07 Всесоюзный научно-исследовательский и испытательный институт медицинской техники Device for determining microorganism motility
WO1995008640A1 (en) * 1993-09-23 1995-03-30 E.I. Du Pont De Nemours And Company An electrophoretic method for the isolation and separation of microorganisms
WO1999060659A1 (en) * 1998-05-15 1999-11-25 Raytheon Company Adaptive antenna pattern control for a multiple access communication system

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
ACTA ODONTOLOGICA SCANDINAVICA, (1988 JUN) 46 (3) 177-80. *
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, (1997 MAY) 63 (5) 1725-31. *
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, (1999 JUL) 65 (7) 3222-5. *
COLLOIDS SURF., B (1995), 4(2), 87-95 *
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; YELLOJI RAO, M. K. ET AL: "Electrokinetic properties of Streptococcus sanguis and Actinomyces naeslundii", XP002149287, retrieved from STN Database accession no. 122:182863 *
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; KAMBARA M ET AL: "Zeta potential of oral bacteria (Streptococci).", XP002149286, retrieved from STN Database accession no. 90347581 *
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; LYTLE D A ET AL: "Electrophoretic mobilities of Escherichia coli O157:H7 and wild-type Escherichia coli strains.", XP002149308, retrieved from STN Database accession no. 1999318683 *
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; PELLETIER C ET AL: "Cell surface characteristics of Lactobacillus casei subsp. casei, Lactobacillus paracasei subsp. paracasei, and Lactobacillus rhamnosus strains.", XP002149307, retrieved from STN Database accession no. 97288068 *
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; SIMONSSON T ET AL: "Influence of pH on the suspension stability of some oral bacteria.", XP002149288, retrieved from STN Database accession no. 88306784 *
DATABASE WPI Derwent World Patents Index; AN 1992-314776, XP002149289 *
JOURNAL OF OSAKA DENTAL UNIVERSITY, (1989 APR) 23 (1) 39-43. *

Also Published As

Publication number Publication date
GB2348504A (en) 2000-10-04
GB0007771D0 (en) 2000-05-17
GB2348504B (en) 2001-11-28
AU3568100A (en) 2000-10-16
WO2000058503A2 (en) 2000-10-05

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