WO2000057178A3 - Cell detection using small volume elements - Google Patents

Cell detection using small volume elements Download PDF

Info

Publication number
WO2000057178A3
WO2000057178A3 PCT/US2000/006408 US0006408W WO0057178A3 WO 2000057178 A3 WO2000057178 A3 WO 2000057178A3 US 0006408 W US0006408 W US 0006408W WO 0057178 A3 WO0057178 A3 WO 0057178A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
living cells
implementation
small volume
cells
Prior art date
Application number
PCT/US2000/006408
Other languages
French (fr)
Other versions
WO2000057178A2 (en
Inventor
Geoffrey A Dafforn
Rajesh D Patel
Nurith Kurn
Original Assignee
Dade Behring Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Behring Inc filed Critical Dade Behring Inc
Publication of WO2000057178A2 publication Critical patent/WO2000057178A2/en
Publication of WO2000057178A3 publication Critical patent/WO2000057178A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Ecology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Disclosed is a system for detecting living cells in a fluid comprising or suspected of comprising the living cells. In one aspect, the system comprises: a) a first implementation adapted to receive the fluid and to render at least a portion of any living cells in the fluid suitable for concentration; b) a second implementation adapted to receive at least a portion of the fluid from the first implementation, wherein the second implementation comprises an array of individual elements adapted to concentrate the living cells from the fluid and to reduce diffusion of the fluid between each element, the system being further adapted to disperse the living cells from the first implementation into each of the individual elements; and c) a detector operably linked to each individual element in the array, the detector being capable of registering elements having at least one living cell therein, wherein, detection of cells by the system is indicative of the presence of the living cells in the fluid. Additionally disclosed are methods for using the system to detect living cells in a fluid of interest such as blood or a blood-related product such as plasma.
PCT/US2000/006408 1999-03-19 2000-03-13 Cell detection using small volume elements WO2000057178A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US12528299P 1999-03-19 1999-03-19
US60/125,282 1999-03-19
US51216500A 2000-02-24 2000-02-24
US09/512,165 2000-02-24

Publications (2)

Publication Number Publication Date
WO2000057178A2 WO2000057178A2 (en) 2000-09-28
WO2000057178A3 true WO2000057178A3 (en) 2001-08-09

Family

ID=26823426

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/006408 WO2000057178A2 (en) 1999-03-19 2000-03-13 Cell detection using small volume elements

Country Status (1)

Country Link
WO (1) WO2000057178A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL1021258C2 (en) * 2002-08-12 2004-02-17 Tno Method and device for determining the number of living cells in a test fluid and the use thereof.
EP2373975B1 (en) 2008-12-19 2020-04-01 3M Innovative Properties Company System and method for processing samples
CN102301220B (en) 2008-12-19 2014-06-25 3M创新有限公司 System And Method For Concentrating Samples
WO2013003309A1 (en) 2011-06-30 2013-01-03 3M Innovative Properties Company Systems and methods for detecting an analyte of interest in a sample using filters and microstructured surfaces
WO2013003308A1 (en) 2011-06-30 2013-01-03 3M Innovative Properties Company Systems and methods for detecting an analyte of interest in a sample using microstructured surfaces

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985002201A1 (en) * 1983-11-08 1985-05-23 Scientific Diagnostics, Inc. System and methods for cell selection
EP0751393A2 (en) * 1995-06-27 1997-01-02 Becton, Dickinson and Company Method and apparatus for detecting microorganisms
US5837200A (en) * 1995-06-02 1998-11-17 Bayer Aktiengesellschaft Sorting device for biological cells or viruses

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985002201A1 (en) * 1983-11-08 1985-05-23 Scientific Diagnostics, Inc. System and methods for cell selection
US5837200A (en) * 1995-06-02 1998-11-17 Bayer Aktiengesellschaft Sorting device for biological cells or viruses
EP0751393A2 (en) * 1995-06-27 1997-01-02 Becton, Dickinson and Company Method and apparatus for detecting microorganisms

Also Published As

Publication number Publication date
WO2000057178A2 (en) 2000-09-28

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