WO2000056878A1 - Methods of obtaining full-length nucleic acid sequences using e. coli topoisomerase iii and its homologs - Google Patents
Methods of obtaining full-length nucleic acid sequences using e. coli topoisomerase iii and its homologs Download PDFInfo
- Publication number
- WO2000056878A1 WO2000056878A1 PCT/US2000/006560 US0006560W WO0056878A1 WO 2000056878 A1 WO2000056878 A1 WO 2000056878A1 US 0006560 W US0006560 W US 0006560W WO 0056878 A1 WO0056878 A1 WO 0056878A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- mrna
- cdna
- expression vector
- sequence
- tag
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU33978/00A AU3397800A (en) | 1999-03-19 | 2000-03-13 | Methods of obtaining full-length nucleic acid sequences using (e. coli) topoisomerase iii and its homologs |
CA002367723A CA2367723A1 (en) | 1999-03-19 | 2000-03-13 | Methods of obtaining full-length nucleic acid sequences using e. coli topoisomerase iii and its homologs |
JP2000606737A JP2002539784A (en) | 1999-03-19 | 2000-03-13 | Method for obtaining full-length nucleic acid sequence using E. coli topoisomerase III and homologs thereof |
EP00912216A EP1165768A1 (en) | 1999-03-19 | 2000-03-13 | METHODS OF OBTAINING FULL-LENGTH NUCLEIC ACID SEQUENCES USING $i(E. COLI) TOPOISOMERASE III AND ITS HOMOLOGS |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12512699P | 1999-03-19 | 1999-03-19 | |
US60/125,126 | 1999-03-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000056878A1 true WO2000056878A1 (en) | 2000-09-28 |
Family
ID=22418306
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2000/006560 WO2000056878A1 (en) | 1999-03-19 | 2000-03-13 | Methods of obtaining full-length nucleic acid sequences using e. coli topoisomerase iii and its homologs |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1165768A1 (en) |
JP (1) | JP2002539784A (en) |
AU (1) | AU3397800A (en) |
CA (1) | CA2367723A1 (en) |
WO (1) | WO2000056878A1 (en) |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001004289A1 (en) * | 1999-07-13 | 2001-01-18 | Incyte Pharmaceuticals, Inc. | METHODS AND COMPOSITIONS FOR PRODUCING FULL LENGTH cDNA LIBRARIES |
WO2002028876A2 (en) * | 2000-10-05 | 2002-04-11 | Riken | Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers. |
US6548277B1 (en) | 1994-12-19 | 2003-04-15 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
US6653106B1 (en) | 1997-06-12 | 2003-11-25 | Sloan-Kettering Institute For Cancer Research | Topoisomerase-based ligation and cloning methods |
US6916632B2 (en) | 2000-08-21 | 2005-07-12 | Invitrogen Corporation | Methods and reagents for molecular cloning |
US7033801B2 (en) | 2000-12-08 | 2006-04-25 | Invitrogen Corporation | Compositions and methods for rapidly generating recombinant nucleic acid molecules |
US7078501B2 (en) | 2000-02-25 | 2006-07-18 | Invitrogen Corporation | Topoisomerase linker-mediated amplification methods |
US8207318B2 (en) | 2000-12-08 | 2012-06-26 | Life Technologies Corporation | Methods and compositions for generating recombinant nucleic acid molecules |
US8945884B2 (en) | 2000-12-11 | 2015-02-03 | Life Technologies Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites |
US9534252B2 (en) | 2003-12-01 | 2017-01-03 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0373914A2 (en) * | 1988-12-13 | 1990-06-20 | THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING (McGILL UNIVERSITY) | Isolation of full-length cDNA clones and capped mRNA |
EP0625572A1 (en) * | 1992-09-25 | 1994-11-23 | The Kanagawa Academy Of Science And Technology Foundation | PROCESS FOR SYNTHESIZING COMPLETE-LENGTH cDNA, PROCESS FOR PRODUCING INTERMEDIATE THEREFOR, AND PROCESS FOR PRODUCING RECOMBINANT VECTOR CONTAINING COMPLETE-LENGTH cDNA |
WO1996019497A1 (en) * | 1994-12-19 | 1996-06-27 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia dna topoisomerase |
WO1996034981A2 (en) * | 1995-05-02 | 1996-11-07 | Genset Sa | METHOD FOR THE SPECIFIC COUPLING OF THE CAP OF THE EXTREMITY 5' OF A FRAGMENT mRNA AND PREPARATION OF mRNA AND COMPLETE cDNA |
WO1997024455A2 (en) * | 1996-01-03 | 1997-07-10 | Clontech Laboratories, Inc. | METHODS AND COMPOSITIONS FOR FULL-LENGTH cDNA CLONING |
WO1998020122A1 (en) * | 1996-11-01 | 1998-05-14 | The Institute Of Physical And Chemical Research | METHOD FOR FORMING FULL-LENGTH cDNA LIBRARY |
WO1998055502A1 (en) * | 1997-06-04 | 1998-12-10 | Smithkline Beecham Corporation | METHODS FOR RAPID CLONING OF FULL LENGTH cDNAs |
WO1998056943A1 (en) * | 1997-06-12 | 1998-12-17 | Sloan-Kettering Institute For Cancer Research | Covalent joining of dna strands to rna strands catalyzed by vaccinia topoisomerase |
-
2000
- 2000-03-13 JP JP2000606737A patent/JP2002539784A/en active Pending
- 2000-03-13 EP EP00912216A patent/EP1165768A1/en not_active Withdrawn
- 2000-03-13 CA CA002367723A patent/CA2367723A1/en not_active Abandoned
- 2000-03-13 WO PCT/US2000/006560 patent/WO2000056878A1/en active Search and Examination
- 2000-03-13 AU AU33978/00A patent/AU3397800A/en not_active Abandoned
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0373914A2 (en) * | 1988-12-13 | 1990-06-20 | THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING (McGILL UNIVERSITY) | Isolation of full-length cDNA clones and capped mRNA |
EP0625572A1 (en) * | 1992-09-25 | 1994-11-23 | The Kanagawa Academy Of Science And Technology Foundation | PROCESS FOR SYNTHESIZING COMPLETE-LENGTH cDNA, PROCESS FOR PRODUCING INTERMEDIATE THEREFOR, AND PROCESS FOR PRODUCING RECOMBINANT VECTOR CONTAINING COMPLETE-LENGTH cDNA |
WO1996019497A1 (en) * | 1994-12-19 | 1996-06-27 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia dna topoisomerase |
WO1996034981A2 (en) * | 1995-05-02 | 1996-11-07 | Genset Sa | METHOD FOR THE SPECIFIC COUPLING OF THE CAP OF THE EXTREMITY 5' OF A FRAGMENT mRNA AND PREPARATION OF mRNA AND COMPLETE cDNA |
WO1997024455A2 (en) * | 1996-01-03 | 1997-07-10 | Clontech Laboratories, Inc. | METHODS AND COMPOSITIONS FOR FULL-LENGTH cDNA CLONING |
WO1998020122A1 (en) * | 1996-11-01 | 1998-05-14 | The Institute Of Physical And Chemical Research | METHOD FOR FORMING FULL-LENGTH cDNA LIBRARY |
WO1998055502A1 (en) * | 1997-06-04 | 1998-12-10 | Smithkline Beecham Corporation | METHODS FOR RAPID CLONING OF FULL LENGTH cDNAs |
WO1998056943A1 (en) * | 1997-06-12 | 1998-12-17 | Sloan-Kettering Institute For Cancer Research | Covalent joining of dna strands to rna strands catalyzed by vaccinia topoisomerase |
Non-Patent Citations (9)
Title |
---|
A. HAGHIGHAT AND N. SONENBERG: "eIF4G dramatically enhances the binding of eIF4E to the mRNA 5'-Cap structure", J. BIOL. CHEM., vol. 272, no. 35, 29 August 1997 (1997-08-29), AM. SOC. BIOCHEM. MOL.BIOL.,INC.,BALTIMORE,US, pages 21677 - 21680, XP002141735 * |
A. HAGHIGHAT ET AL.: "The eIF4G-eIF4E complex is the target for direct cleavage by the rhinovirus 2A proteinase", J. VIROLOGY, vol. 70, no. 12, December 1996 (1996-12-01), AM.SOC.MICROBIOL.,WASHINGTON,US, pages 8444 - 8450, XP002141736 * |
CARNINCI P ET AL: "High-efficiency full-length cDNA cloning by biotinylated CAP trapper", GENOMICS,US,ACADEMIC PRESS, SAN DIEGO, vol. 37, no. 3, 1 November 1996 (1996-11-01), pages 327 - 336, XP002081729, ISSN: 0888-7543 * |
EDERY I ET AL: "AN EFFICIENT STRATEGY TO ISOLATE FULL-LENGTH CDNAS BASED ON AN MRNA CAP RETENTION PROCEDURE (CAPTURE)", MOLECULAR AND CELLULAR BIOLOGY,US,WASHINGTON, DC, vol. 15, June 1995 (1995-06-01), pages 3363 - 3371, XP002915963, ISSN: 0270-7306 * |
INVITROGEN PRODUCT KATALOG 1998, Carlsbad, CA, US, pages 18,29,43,44,49-52, XP002141734 * |
KATO S ET AL: "CONSTRUCTION OF A HUMAN FULL-LENGTH CDNA BANK", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 150, 1994, pages 243 - 250, XP002915961, ISSN: 0378-1119 * |
MARUYAMA K ET AL: "OLIGO-CAPPING: A SIMPLE METHOD TO REPLACE THE CAP STRUCTURE OF EUROKARYOTIC MRNAS WITH OLIGONUCLEOTIDES", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 138, 1 January 1994 (1994-01-01), pages 171 - 174, XP002017391, ISSN: 0378-1119 * |
P. CARNINCI ET AL.: "High efficiency selection of full-length cDNA by improved biotinylated cap trapper", DNA RESEARCH, vol. 4, 1997, KAZUSA DNA RES. UNIV.ACAD.PRESS,INC.,TOKYO,JP, pages 61 - 66, XP002915968 * |
R J DIGATE ET AL: "Molecular Cloning and DNA Sequence Analysis of Escherichia coli topB, the gene encoding Topoisomerase III", JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 264, no. 30, 25 October 1989 (1989-10-25), pages 17924 - 17930-17930, XP002122019, ISSN: 0021-9258 * |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7550295B2 (en) | 1994-12-19 | 2009-06-23 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
US8551739B2 (en) | 1994-12-19 | 2013-10-08 | Memorial Sloan-Kettering Cancer Center | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
US6548277B1 (en) | 1994-12-19 | 2003-04-15 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
US7026141B2 (en) | 1994-12-19 | 2006-04-11 | Sloan-Kettering Institute For Cancer Research | Topoisomerase-based reagents and methods for molecular cloning |
US8192932B2 (en) | 1997-06-12 | 2012-06-05 | Sloan-Kettering Institute For Cancer Research | Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase |
US6653106B1 (en) | 1997-06-12 | 2003-11-25 | Sloan-Kettering Institute For Cancer Research | Topoisomerase-based ligation and cloning methods |
US7662556B2 (en) | 1997-06-12 | 2010-02-16 | Sloan Kettering Institute For Cancer Research | Covalent joining of DNA to RNA by vaccinia topoisomerase and uses thereof |
WO2001004289A1 (en) * | 1999-07-13 | 2001-01-18 | Incyte Pharmaceuticals, Inc. | METHODS AND COMPOSITIONS FOR PRODUCING FULL LENGTH cDNA LIBRARIES |
US7078501B2 (en) | 2000-02-25 | 2006-07-18 | Invitrogen Corporation | Topoisomerase linker-mediated amplification methods |
US6916632B2 (en) | 2000-08-21 | 2005-07-12 | Invitrogen Corporation | Methods and reagents for molecular cloning |
US7528241B2 (en) | 2000-08-21 | 2009-05-05 | Life Technologies Corporation | Methods and reagents for molecular cloning |
US8883991B2 (en) | 2000-08-21 | 2014-11-11 | Life Technologies Corporation | Methods and reagents for molecular cloning |
US9309520B2 (en) | 2000-08-21 | 2016-04-12 | Life Technologies Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
WO2002028876A3 (en) * | 2000-10-05 | 2002-08-01 | Riken | Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers. |
WO2002028876A2 (en) * | 2000-10-05 | 2002-04-11 | Riken | Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers. |
US8809518B2 (en) | 2000-10-05 | 2014-08-19 | Riken | Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers |
US7033801B2 (en) | 2000-12-08 | 2006-04-25 | Invitrogen Corporation | Compositions and methods for rapidly generating recombinant nucleic acid molecules |
US8207318B2 (en) | 2000-12-08 | 2012-06-26 | Life Technologies Corporation | Methods and compositions for generating recombinant nucleic acid molecules |
US8945884B2 (en) | 2000-12-11 | 2015-02-03 | Life Technologies Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites |
US9534252B2 (en) | 2003-12-01 | 2017-01-03 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
Also Published As
Publication number | Publication date |
---|---|
AU3397800A (en) | 2000-10-09 |
CA2367723A1 (en) | 2000-09-28 |
EP1165768A1 (en) | 2002-01-02 |
JP2002539784A (en) | 2002-11-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU746657B2 (en) | Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase | |
US20070128724A1 (en) | System for the rapid manipulation of nuculeic acid sequences | |
Xu et al. | Intein-mediated ligation and cyclization of expressed proteins | |
KR20190059966A (en) | S. The Piogenes CAS9 mutant gene and the polypeptide encoded thereby | |
GERARD et al. | Influence on stability in Escherichia coli of the carboxy-terminal structure of cloned Moloney murine leukemia virus reverse transcriptase | |
WO2000056878A1 (en) | Methods of obtaining full-length nucleic acid sequences using e. coli topoisomerase iii and its homologs | |
JP7258361B2 (en) | Cell-free protein expression using double-stranded concatemer DNA | |
EP3976770A1 (en) | Methods and compositions for manufacturing polynucleotides | |
CN114606215B (en) | Argonaute protein derived from eukaryote and application thereof | |
AU2020209757B2 (en) | A method for assembling circular and linear DNA molecules in an ordered manner | |
JP4808361B2 (en) | New DNA synthase | |
CN115197300B (en) | Protein with non-sequence specificity and high affinity for RNA and application thereof | |
WO2022210748A1 (en) | Novel polypeptide having ability to form complex with guide rna | |
JPWO2006123537A1 (en) | Novel endoribonuclease | |
JP2006180886A (en) | Method for producing dna polymerase | |
JP2002510502A (en) | Method for producing a library of expressible gene sequences | |
PAQUETTE et al. | Dcp1–Dcp2 decapping enzyme for transcription start site mapping | |
RU2020137717A (en) | COMPOSITIONS AND METHODS FOR GENE EDITING | |
AU2002300348A9 (en) | Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
ENP | Entry into the national phase |
Ref document number: 2367723 Country of ref document: CA Kind code of ref document: A Ref document number: 2000 606737 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 514308 Country of ref document: NZ |
|
WWE | Wipo information: entry into national phase |
Ref document number: 33978/00 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2000912216 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 2000912216 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 09937112 Country of ref document: US |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 2000912216 Country of ref document: EP |
|
DPE2 | Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101) |