WO2000053616A3 - Nucleotides and polynucleotides immobilised on a solid support via a disulphide linkage - Google Patents

Nucleotides and polynucleotides immobilised on a solid support via a disulphide linkage Download PDF

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Publication number
WO2000053616A3
WO2000053616A3 PCT/GB2000/000843 GB0000843W WO0053616A3 WO 2000053616 A3 WO2000053616 A3 WO 2000053616A3 GB 0000843 W GB0000843 W GB 0000843W WO 0053616 A3 WO0053616 A3 WO 0053616A3
Authority
WO
WIPO (PCT)
Prior art keywords
solid support
nucleotides
support via
polynucleotide
nucleotide
Prior art date
Application number
PCT/GB2000/000843
Other languages
French (fr)
Other versions
WO2000053616A2 (en
Inventor
Angelika Josefine Rosler
Original Assignee
Amersham Pharm Biotech Uk Ltd
Angelika Josefine Rosler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharm Biotech Uk Ltd, Angelika Josefine Rosler filed Critical Amersham Pharm Biotech Uk Ltd
Priority to AU29308/00A priority Critical patent/AU2930800A/en
Priority to EP00907844A priority patent/EP1259524A2/en
Priority to CA002365226A priority patent/CA2365226A1/en
Priority to JP2000604051A priority patent/JP2003520766A/en
Publication of WO2000053616A2 publication Critical patent/WO2000053616A2/en
Publication of WO2000053616A3 publication Critical patent/WO2000053616A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method of immobilising a nucleotide or polynucleotide on a solid support, comprises: providing the solid support having a functionalised surface of the formula B - S - S - L where B is the solid support and L is a leaving group; providing a nucleotide or polynucleotide comprising at least one phosphorothioate group; and coupling the two together by means of a sulphide exchange reaction. The product has the structure B - S - S - P(O)R1R2, where - POR1R2 represents a nucleotide or a polynucleotide. Arrays of the immobilised polynucleotides are useful for performing APEX or SBH assays.
PCT/GB2000/000843 1999-03-09 2000-03-07 Nucleotides and polynucleotides immobilised on a solid support via a disulphide linkage WO2000053616A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU29308/00A AU2930800A (en) 1999-03-09 2000-03-07 Immobilising polynucleotides
EP00907844A EP1259524A2 (en) 1999-03-09 2000-03-07 Nucleotides and polynucleotides immobilised on a solid support via a disulphide linkage
CA002365226A CA2365226A1 (en) 1999-03-09 2000-03-07 Immobilising polynucleotides
JP2000604051A JP2003520766A (en) 1999-03-09 2000-03-07 Fixed polynucleotide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99301764.9 1999-03-09
EP99301764 1999-03-09

Publications (2)

Publication Number Publication Date
WO2000053616A2 WO2000053616A2 (en) 2000-09-14
WO2000053616A3 true WO2000053616A3 (en) 2002-09-12

Family

ID=8241258

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/000843 WO2000053616A2 (en) 1999-03-09 2000-03-07 Nucleotides and polynucleotides immobilised on a solid support via a disulphide linkage

Country Status (5)

Country Link
EP (1) EP1259524A2 (en)
JP (1) JP2003520766A (en)
AU (1) AU2930800A (en)
CA (1) CA2365226A1 (en)
WO (1) WO2000053616A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030143331A1 (en) * 2001-10-03 2003-07-31 Micrologix Biotech Inc. Attachment of thiophosphate tethered oligonucleotides to a solid surface
DE10159904A1 (en) * 2001-12-06 2003-07-03 Adnagen Ag Oligonucleotide arrangement, method for nucleotide detection and device therefor
GB0326073D0 (en) 2003-11-07 2003-12-10 Solexa Ltd Improvements in or relating to polynucleotide arrays
US8067164B2 (en) 2007-08-12 2011-11-29 Integrated Dna Technologies, Inc. Microarray system with improved sequence specificity

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990001564A1 (en) * 1988-08-09 1990-02-22 Microprobe Corporation Methods for multiple target analyses through nucleic acid hybridization
WO1998039481A1 (en) * 1997-03-05 1998-09-11 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990001564A1 (en) * 1988-08-09 1990-02-22 Microprobe Corporation Methods for multiple target analyses through nucleic acid hybridization
WO1998039481A1 (en) * 1997-03-05 1998-09-11 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHASSIGNOL M ET AL: "Phosphodisulfide Bond: A New Linker for the Oligonucleotide Conjugation", TETRAHEDRON LETTERS, vol. 39, no. 45, 5 November 1998 (1998-11-05), pages 8271-8274, XP004139391, ISSN: 0040-4039 *
H.SALO ET AL.: "Disulfide-Tethered Solid Supports for Synthesis of Photoluminescent Oligonucleotide Conjugates : Hydrolytic Stability and Labelling on the Support.", BIOCONJUGATE CHEMISTRY., vol. 9, no. 3, May 1998 (1998-05-01) - June 1998 (1998-06-01), AMERICAN CHEMICAL SOCIETY, WASHINGTON., US, pages 365 - 371, XP002112924, ISSN: 1043-1802 *
MILLOT M C ET AL: "HPLC DETERMINATION OF BIOLOGICAL THIOLS USING POLYMER-COATED SOLID -PHASE REAGENTS CARRYING DISULFIDE FUNCTIONS", REACTIVE POLYMERS, vol. 13, no. 1 + 02, 1 September 1990 (1990-09-01), pages 177 - 190, XP000160693, ISSN: 0923-1137 *
P.KUMAR ET AL.: "A Versatile Solid Phase Method for the Synthesis of Oligonucleotide-3'-Phosphates.", TETRAHEDRON LETTERS., vol. 32, no. 6, 4 February 1991 (1991-02-04), ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM., NL, pages 967 - 970, XP002112931, ISSN: 0040-4039 *
S.ALEFELDER ET AL.: "Incorporation of Terminal Phosphorothioates into Oligonucleotides.", NUCLEIC ACIDS RESEARCH., vol. 26, no. 21, 1 November 1998 (1998-11-01), OXFORD UNIVERSITY PRESS, SURREY., GB, pages 4983 - 4988, XP002112925, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2000053616A2 (en) 2000-09-14
JP2003520766A (en) 2003-07-08
AU2930800A (en) 2000-09-28
EP1259524A2 (en) 2002-11-27
CA2365226A1 (en) 2000-09-14

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