WO2000050643A3 - Method for detecting radiation exposure - Google Patents

Method for detecting radiation exposure Download PDF

Info

Publication number
WO2000050643A3
WO2000050643A3 PCT/US2000/004897 US0004897W WO0050643A3 WO 2000050643 A3 WO2000050643 A3 WO 2000050643A3 US 0004897 W US0004897 W US 0004897W WO 0050643 A3 WO0050643 A3 WO 0050643A3
Authority
WO
WIPO (PCT)
Prior art keywords
genes
nucleic acid
radiation
exposed
radiation exposure
Prior art date
Application number
PCT/US2000/004897
Other languages
French (fr)
Other versions
WO2000050643A2 (en
Inventor
Albert J Fornace Jr
Sally A Amundson
Jeffrey M Trent
Original Assignee
Us Gov Health & Human Serv
Albert J Fornace Jr
Sally A Amundson
Jeffrey M Trent
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv, Albert J Fornace Jr, Sally A Amundson, Jeffrey M Trent filed Critical Us Gov Health & Human Serv
Priority to US09/913,171 priority Critical patent/US7008768B1/en
Priority to AU38610/00A priority patent/AU3861000A/en
Publication of WO2000050643A2 publication Critical patent/WO2000050643A2/en
Publication of WO2000050643A3 publication Critical patent/WO2000050643A3/en
Priority to US11/370,079 priority patent/US7563573B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/142—Toxicological screening, e.g. expression profiles which identify toxicity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method is disclosed for detecting exposure of organisms to biologically significant or hazardous amounts of ionizing radiation. The method uses nucleic acid microarray hybridization to evaluate biological effects, such as patterns of expression of genes after radiation exposure. Numerous genes are provided which have been found to be responsive to radiation exposure in a variety of cell lines. These genes are incorporated into probe sets, which are exposed to a labeled nucleic acid composition from a test cell, such as cDNA reverse transcribed from mRNA in the test cell, which specifically hybridizes to members of the probe set when the cell has been exposed to a biologically significant amount of ionizing radiation. Whether the nucleic acid composition hybridizes to the nucleic acid molecules representing genes that are differentially expressed is determined. The invention also includes methods for determining a dose response relationship between radiation exposure and differential expression of one or more genes, for example to determine a probable radiation dose in cells that have actually or potentially been exposed to the ionizing radiation. The invention also includes probe sets and microarrays used in this method.
PCT/US2000/004897 1999-02-26 2000-02-25 Method for detecting radiation exposure WO2000050643A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US09/913,171 US7008768B1 (en) 1999-02-26 2000-02-25 Method for detecting radiation exposure
AU38610/00A AU3861000A (en) 1999-02-26 2000-02-25 Method for detecting radiation exposure
US11/370,079 US7563573B2 (en) 1999-02-26 2006-03-06 Method for detecting radiation exposure

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12175699P 1999-02-26 1999-02-26
US60/121,756 1999-02-26

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09913171 A-371-Of-International 2000-02-25
US11/370,079 Division US7563573B2 (en) 1999-02-26 2006-03-06 Method for detecting radiation exposure

Publications (2)

Publication Number Publication Date
WO2000050643A2 WO2000050643A2 (en) 2000-08-31
WO2000050643A3 true WO2000050643A3 (en) 2001-07-12

Family

ID=22398603

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/004897 WO2000050643A2 (en) 1999-02-26 2000-02-25 Method for detecting radiation exposure

Country Status (2)

Country Link
AU (1) AU3861000A (en)
WO (1) WO2000050643A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7003403B1 (en) 2000-06-15 2006-02-21 The United States Of America As Represented By The Department Of Health And Human Services Quantifying gene relatedness via nonlinear prediction of gene
US20050048491A1 (en) * 2001-10-09 2005-03-03 WEICHSELBAUM Ralph R. Methods and kits for use in selecting approaches to treating cancer
US7879545B2 (en) * 2003-11-05 2011-02-01 H. Lee Moffitt Cancer Center And Research Institute, Inc. Identification of novel targets for radio sensitization using a genomic-based radiation sensitivity classifier
DE10357677A1 (en) * 2003-12-10 2005-07-14 Greiner Bio-One Gmbh Primers and probes for detecting genital HPV genotypes
JP2008517601A (en) * 2004-10-22 2008-05-29 プロメガ コーポレイション Method and kit for detecting germ cell genomic instability
US9255348B2 (en) * 2006-08-25 2016-02-09 The Trustees Of Columbia University In The City Of New York Systems and methods for biodosimetry with biochip using gene expression signatures
US20080234946A1 (en) 2007-03-22 2008-09-25 University Of South Florida Predictive radiosensitivity network model
US20090076734A1 (en) 2007-03-22 2009-03-19 Torres-Roca Javier F Gene Signature for the Prediction of Radiation Therapy Response
WO2011006214A1 (en) * 2009-07-16 2011-01-20 Peter Maccallum Cancer Institute Method of detecting radiation exposure and adverse toxicity thereto
US10370712B2 (en) 2012-09-24 2019-08-06 Duke University Signatures of radiation response
JP2020517580A (en) * 2017-05-17 2020-06-18 シェンチェン・インターナショナル・インスティテュート・フォー・バイオメディカル・リサーチＳｈｅｎｚｈｅｎ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｉｎｓｔｉｔｕｔｅ ｆｏｒ Ｂｉｏｍｅｄｉｃａｌ Ｒｅｓｅａｒｃｈ Methods and compositions for treating ionizing radiation resistant tumors
CN111621559A (en) * 2020-06-11 2020-09-04 北京市化工职业病防治院(北京市职业病防治研究院) Screening of Low dose ionizing radiation Effector genes in Association of transcriptome and proteome data

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999055913A2 (en) * 1998-04-27 1999-11-04 Sidney Kimmel Cancer Center Reduced complexity nucleic acid targets and methods of using same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999055913A2 (en) * 1998-04-27 1999-11-04 Sidney Kimmel Cancer Center Reduced complexity nucleic acid targets and methods of using same

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; AMUNDSON, S. A. ET AL: "Identification of gamma-ray responsive genes by cDNA array hybridization.", XP002155693, retrieved from STN *
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; AMUNDSON, SALLY A. ET AL: "Induction of stress genes by low doses of gamma rays", XP002155694, retrieved from STN Database accession no. 131:283349 HCA *
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; AMUNDSON S A ET AL: "Fluorescent cDNA microarray hybridization reveals complexity and heterogeneity of cellular genotoxic stress responses.", XP002155695, retrieved from STN Database accession no. 1999308597 *
DERISI ET AL: "Use of a cDNA microarray to analyse gene expression patterns in human cancer", NATURE GENETICS, vol. 14, December 1996 (1996-12-01), pages 457 - 460, XP000971491 *
IORDANOV, MIHAIL S. ET AL: "Ultraviolet radiation triggers the ribotoxic stress response in mammalian cells.", JOURNAL OF BIOLOGICAL CHEMISTRY, (JUNE 19, 1998) VOL. 273, NO. 25, PP. 15794-15803., XP002155692 *
ONCOGENE, (1999 JUN 17) 18 (24) 3666-72. *
PRASAD ET AL: "Activation of nuclear factor kB in human lymphoblastoid cells by low dose ionizing radiation", RADIATION RES, vol. 138, 1994, pages 367 - 372, XP002155691 *
PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH ANNUAL MEETING, (MARCH, 1998) VOL. 39, PP. 454. MEETING INFO.: 89TH ANNUAL MEETING OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH NEW ORLEANS, LOUISIANA, USA MARCH 28-APRIL 1, 1998 AMERICAN *
RADIAT. RES. (1999), 152(3), 225-231 *

Also Published As

Publication number Publication date
AU3861000A (en) 2000-09-14
WO2000050643A2 (en) 2000-08-31

Similar Documents

Publication Publication Date Title
Pantel et al. Species-specific alternative splice mimicry at the growth hormone receptor locus revealed by the lineage of retroelements during primate evolution: a novel mechanism accounting for protein diversity between and within species
Jiang et al. Real-time expression profiling of microRNA precursors in human cancer cell lines
Slaby et al. Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer
Choi et al. Multiplexed detection of mRNA using porosity-tuned hydrogel microparticles
Varilo et al. The interval of linkage disequilibrium (LD) detected with microsatellite and SNP markers in chromosomes of Finnish populations with different histories
Wu et al. Development and evaluation of microarray-based whole-genome hybridization for detection of microorganisms within the context of environmental applications
Ronald et al. Simultaneous genotyping, gene-expression measurement, and detection of allele-specific expression with oligonucleotide arrays
Meloni et al. A tetranucleotide polymorphic microsatellite, located in the first intron of the tyrosine hydroxylase gene, acts as a transcription regulatory element in vitro
Cox et al. A survey of homozygous deletions in human cancer genomes
Theodorakis et al. Genetic ecotoxicology II: population genetic structure in mosquitofish exposed in situ to radionuclides
Chen et al. Highly sensitive and specific microRNA expression profiling using BeadArray technology
Duitama et al. Large-scale analysis of tandem repeat variability in the human genome
Hehir-Kwa et al. Genome-wide copy number profiling on high-density bacterial artificial chromosomes, single-nucleotide polymorphisms, and oligonucleotide microarrays: a platform comparison based on statistical power analysis
CN106544419B (en) Primer, probe and kit for detecting ApoE and SLCO1B1 gene polymorphism
Driggers et al. Mapping frequencies of endogenous oxidative damage and the kinetic response to oxidative stress in a region of rat mtDNA
WO2000050643A3 (en) Method for detecting radiation exposure
WO2000077260A8 (en) Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
WO2002048310A3 (en) Methods and compositions for diagnosing and treating rheumatoid arthritis
CN101448955A (en) Method for identifying altered vitamin D metabolism
WO2009099905A2 (en) Molecular signatures and biomarkers associated with melanoma and methods of use thereof
Liang et al. The mutation spectrum of the phenylalanine hydroxylase (PAH) gene and associated haplotypes reveal ethnic heterogeneity in the Taiwanese population
EP2185729A1 (en) Method of identifying individuals at risk of thiopurine drug resistance and intolerance
Gribble et al. Linkage analysis of urine arsenic species patterns in the strong heart family study
Ding et al. The methodology used to measure differential gene expression affects the outcome
Van Leeuwen et al. Transcriptome analysis in peripheral blood of humans exposed to environmental carcinogens: a promising new biomarker in environmental health studies

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

ENP Entry into the national phase

Ref country code: US

Ref document number: 2001 913171

Date of ref document: 20010808

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 09913171

Country of ref document: US

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase