WO2000050613A3 - Plantes transgeniques et procede de transformation d'oeillets - Google Patents

Plantes transgeniques et procede de transformation d'oeillets Download PDF

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Publication number
WO2000050613A3
WO2000050613A3 PCT/IL2000/000110 IL0000110W WO0050613A3 WO 2000050613 A3 WO2000050613 A3 WO 2000050613A3 IL 0000110 W IL0000110 W IL 0000110W WO 0050613 A3 WO0050613 A3 WO 0050613A3
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WO
WIPO (PCT)
Prior art keywords
explants
carnation
dna molecule
shoots
transforming
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PCT/IL2000/000110
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English (en)
Other versions
WO2000050613A2 (fr
WO2000050613A8 (fr
Inventor
Alexander Vainstein
Amir Zuker
Marianna Ovadis
Original Assignee
Yissum Res Dev Co
Alexander Vainstein
Amir Zuker
Marianna Ovadis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Res Dev Co, Alexander Vainstein, Amir Zuker, Marianna Ovadis filed Critical Yissum Res Dev Co
Priority to US09/914,146 priority Critical patent/US7129393B1/en
Priority to AU26870/00A priority patent/AU2687000A/en
Publication of WO2000050613A2 publication Critical patent/WO2000050613A2/fr
Publication of WO2000050613A3 publication Critical patent/WO2000050613A3/fr
Publication of WO2000050613A8 publication Critical patent/WO2000050613A8/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne un procédé de transformation du génome d'un oeillet (Dianthus L.) grâce à une molécule d'ADN. Le procédé consiste : (a) à préparer des explants de tiges à partir de boutures d'oeillets ; (b) à pratiquer des lésions sur les explants par bombardement avec des microprojectiles ; (c) à co-cultiver les explants lésés avec Agrobacterium comprenant la molécule d'ADN dans des conditions définies d'exposition à l'obscurité puis à la lumière ; (d) à exciser les pousses provenant des explants lésés et à retirer les feuilles des pousses ; et (e) à mettre en culture les feuilles pour obtenir des pousses transgéniques transformées à l'aide de la molécule d'ADN. L'invention concerne également un oeillet transgénique rolC possédant des caractéristiques agronomiques améliorées et au parfum des fleurs mis en valeur grâce à l'élimination antisens du gène flavonoïde $i(fht.)
PCT/IL2000/000110 1999-02-22 2000-02-22 Plantes transgeniques et procede de transformation d'oeillets WO2000050613A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US09/914,146 US7129393B1 (en) 1999-02-22 2000-02-22 Transgenic plants and method for transforming carnations
AU26870/00A AU2687000A (en) 1999-02-22 2000-02-22 Transgenic plants and method for transforming carnations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12123999P 1999-02-22 1999-02-22
US60/121,239 1999-02-22

Publications (3)

Publication Number Publication Date
WO2000050613A2 WO2000050613A2 (fr) 2000-08-31
WO2000050613A3 true WO2000050613A3 (fr) 2000-12-14
WO2000050613A8 WO2000050613A8 (fr) 2001-02-22

Family

ID=22395426

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2000/000110 WO2000050613A2 (fr) 1999-02-22 2000-02-22 Plantes transgeniques et procede de transformation d'oeillets

Country Status (2)

Country Link
AU (1) AU2687000A (fr)
WO (1) WO2000050613A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19927568A1 (de) * 1999-06-17 2000-12-21 Basf Ag Verfahren zur Erhöhung der Widerstandskraft von Kulturpflanzen gegen chemischen Streß
DE19927574A1 (de) * 1999-06-17 2000-12-21 Basf Ag Verfahren zur Herstellung von Pflanzen mit erhöhtem Gehalt an Flavonoiden und phenolischen Verbindungen
DE19927575A1 (de) * 1999-06-17 2000-12-21 Basf Ag Verfahren zur Erhöhung der Widerstandskraft von Kulturpflanzen gegen phytopathogene Pilze und Bakterien mit Hilfe molekulargenetischer Methoden
US20010044379A1 (en) 1999-12-10 2001-11-22 Alan Blowers Modification of floral scent in flowering plants
US20030088888A1 (en) * 2001-11-06 2003-05-08 Alexander Vainstein Method of plant transformation

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0486233A2 (fr) * 1990-11-14 1992-05-20 Pioneer Hi-Bred International, Inc. Procédé de transformation de plantes utilisants des espèces d'Agrobacterium
WO1992017056A1 (fr) * 1991-04-01 1992-10-15 Florigene B.V. Procedes de transformation et de developpement des ×illets.
WO1993018142A1 (fr) * 1992-03-09 1993-09-16 Washington State University Research Foundation Procede de regulation de la fertilite des plantes
WO1994028140A1 (fr) * 1993-05-20 1994-12-08 International Flower Developments Pty. Ltd. Plantes a fleurs transgeniques
WO1995006741A1 (fr) * 1993-08-30 1995-03-09 Biocem Procede de production de plantes transgeniques, entierement transformees en generation to, a partir de meristemes
WO1996020595A1 (fr) * 1994-12-30 1996-07-11 Proguard, Inc. Modulation du niveau des metabolites toxiques dans les produits de consommation
WO1996036716A1 (fr) * 1995-05-16 1996-11-21 International Flower Developments Pty. Ltd. Plantes transgeniques presentant des couleurs florales modifiees et procedes permettant de les obtenir
WO1996039827A1 (fr) * 1995-06-07 1996-12-19 Proguard, Inc. Compositions repulsives contenant des aldehydes de flavonoides
WO1997015584A2 (fr) * 1995-10-12 1997-05-01 The Regents Of The University Of Michigan Utilisation de synthase de linalol dans le genie genetique de la production d'odeurs
WO1997021816A1 (fr) * 1995-12-13 1997-06-19 Zeneca Limited Modulation genetique du murissement des fruits
WO1997035471A2 (fr) * 1996-03-25 1997-10-02 Proguard, Inc. Utilisation d'aldehydes aromatiques comme pesticides
WO1998050570A2 (fr) * 1997-05-08 1998-11-12 The Regents Of The University Of Michigan Procedes et compositions ayant trait a l'utilisation d'(iso)eugenol methyltransferase
WO1999037794A1 (fr) * 1998-01-26 1999-07-29 Unilever Plc Procede et compositions pour la modulation de la teneur en flavonoides

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0486233A2 (fr) * 1990-11-14 1992-05-20 Pioneer Hi-Bred International, Inc. Procédé de transformation de plantes utilisants des espèces d'Agrobacterium
WO1992017056A1 (fr) * 1991-04-01 1992-10-15 Florigene B.V. Procedes de transformation et de developpement des ×illets.
WO1993018142A1 (fr) * 1992-03-09 1993-09-16 Washington State University Research Foundation Procede de regulation de la fertilite des plantes
WO1994028140A1 (fr) * 1993-05-20 1994-12-08 International Flower Developments Pty. Ltd. Plantes a fleurs transgeniques
WO1995006741A1 (fr) * 1993-08-30 1995-03-09 Biocem Procede de production de plantes transgeniques, entierement transformees en generation to, a partir de meristemes
WO1996020595A1 (fr) * 1994-12-30 1996-07-11 Proguard, Inc. Modulation du niveau des metabolites toxiques dans les produits de consommation
WO1996036716A1 (fr) * 1995-05-16 1996-11-21 International Flower Developments Pty. Ltd. Plantes transgeniques presentant des couleurs florales modifiees et procedes permettant de les obtenir
WO1996039827A1 (fr) * 1995-06-07 1996-12-19 Proguard, Inc. Compositions repulsives contenant des aldehydes de flavonoides
WO1997015584A2 (fr) * 1995-10-12 1997-05-01 The Regents Of The University Of Michigan Utilisation de synthase de linalol dans le genie genetique de la production d'odeurs
WO1997021816A1 (fr) * 1995-12-13 1997-06-19 Zeneca Limited Modulation genetique du murissement des fruits
WO1997035471A2 (fr) * 1996-03-25 1997-10-02 Proguard, Inc. Utilisation d'aldehydes aromatiques comme pesticides
WO1998050570A2 (fr) * 1997-05-08 1998-11-12 The Regents Of The University Of Michigan Procedes et compositions ayant trait a l'utilisation d'(iso)eugenol methyltransferase
WO1999037794A1 (fr) * 1998-01-26 1999-07-29 Unilever Plc Procede et compositions pour la modulation de la teneur en flavonoides

Non-Patent Citations (17)

* Cited by examiner, † Cited by third party
Title
BRITSCH L ET AL: "Molecular characterization of flavone-3[beta]-hydroxylases", EUROPEAN JOURNAL OF BIOCHEMISTRY,DE,BERLIN, vol. 217, no. 2, 15 October 1993 (1993-10-15), pages 745 - 754, XP002108555, ISSN: 0014-2956 *
DATABASE EMBL 16 March 1993 (1993-03-16), BRITSCH L., ET AL.: "D.caryophyllus mRNA for flavanone 3-beta-hydroxylase", XP002149238 *
DATABASE EMBL 23 February 1993 (1993-02-23), DEDIO, J., ET AL.: "D.caryophyllus fht gene for narigenin 3-dioxygenase", XP002149237 *
DEDIO J ET AL: "Molecular cloning of the flavanone 3-beta-hydroxylase gene (FHT) from carnation (Dianthus caryophyllus) and analysis of stable and unstable FHT mutants.", THEORETICAL AND APPLIED GENETICS, vol. 90, no. 5, 1995, pages 611 - 617, XP000915009, ISSN: 0040-5752 *
FIROOZABADY, E., ET AL.: "Efficient transformation and regeneration of carnation cultivars using Agrobacterium", MOLECULAR BREEDING, vol. 1, 1995, pages 283 - 293, XP002140940 *
GUTTERSON NEAL: "Anthocyanin biosynthetic genes and their application to flower color modification through sense suppression.", HORTSCIENCE, vol. 30, no. 5, 1995, pages 964 - 966, XP000922821, ISSN: 0018-5345 *
MSC THESIS. AHRONI A.. 1996 (THE HEBREW UNIVERSITY OF JERUSALEM ISRAEL) *
OVADIS, M., ET AL.: "A highly efficient procedure for generating carnation plants with novel traits", PROCEEDINGS OF THE NINETEENTH INTERNATIONAL SYMPOSIUM ON IMPROVEMENT OF ORNAMENTAL PLANTS. BREEDING ORNAMENTALS IN THE FUTURE: GOALS, GENES, TOOLS, ANGERS, FRANCE, 27-30 JULY, 1998. ACTA HORTICULTURAE, (2000) NO. 508, PP. 49-51., ISSN: 0567-7572; ISBN: 90-6605-832-3, XP000946845 *
OVADIS, M., ET AL.: "Generation of transgenic carnation plants with novel characteristics by combining microprojectile bombardment with Agrobacterium tumefaciens transformation", CURRENT PLANT SCIENCE AND BIOTECHNOLOGY IN AGRICULTURE VOL. 36., Plant biotechnology and in vitro biology in the 21st century. Proceedings of the IXth International Congress of the International Association of Plant Tissue Culture and Biotechnology,Jerusalem, Israe, pages 189 - 192, XP000921439 *
PELLEGRINESCHI A ET AL: "IMPROVEMENT OF ORNAMENTAL CHARACTERS AND FRAGRANCE PRODUCTION IN LEMON-SCENTED GERANIUM THROUGH GENETIC TRANSFORMATION BY AGROBACTERIUM RHIZOGENES", BIO/TECHNOLOGY,US,NATURE PUBLISHING CO. NEW YORK, vol. 12, no. 1, 1994, pages 64 - 68, XP002036390, ISSN: 0733-222X *
TANAKA YOSHIKAZU ET AL: "Metabolic engineering to modify flower color.", PLANT AND CELL PHYSIOLOGY, vol. 39, no. 11, November 1998 (1998-11-01), pages 1119 - 1126, XP000922977, ISSN: 0032-0781 *
WATAD ABED A ET AL: "Adventitious shoot formation from carnation stem segments: A comparison of different culture procedures.", SCIENTIA HORTICULTURAE (AMSTERDAM), vol. 65, no. 4, 1996, pages 313 - 320, XP000921442, ISSN: 0304-4238 *
ZUKER A ET AL: "Genetic engineering for cut-flower improvement", BIOTECHNOLOGY ADVANCES,GB,ELSEVIER PUBLISHING, BARKING, vol. 16, no. 1, 1998, pages 33 - 79, XP004099136, ISSN: 0734-9750 *
ZUKER AMIR ET AL: "Transformation of carnation by microprojectile bombardment.", SCIENTIA HORTICULTURAE (AMSTERDAM), vol. 64, no. 3, 1995, pages 177 - 185, XP000921441, ISSN: 0304-4238 *
ZUKER AMIR ET AL: "Wounding by bombardment yields highly efficient Agrobacterium-mediated transformation of carnation (Dianthus caryophyllus L.).", MOLECULAR BREEDING, vol. 5, no. 4, 1999, pages 367 - 375, XP002140941, ISSN: 1380-3743 *
ZUKER, A., ET AL., ABST. IX. INTERNATIONAL CONGRESS ON PLANT TISSUE AND CELL CULTURE, 1998, pages 35 *
ZUKER, A., ET AL.: "A highly efficient method for carnation transformation", ACTA HORTICULTURAE, (1997) NO. 447, PP. 373-375., Proceedings of the third international ISHS symposium on in vitro culture and horticultural breeding, Jerusalem, Israel,16-21 June, 1996.ISSN: 0567-7572; ISBN: 90-6605-909-5, XP000921438 *

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WO2000050613A2 (fr) 2000-08-31
WO2000050613A8 (fr) 2001-02-22
AU2687000A (en) 2000-09-14

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