WO2000044438A1 - Delivery of macromolecules into cells - Google Patents
Delivery of macromolecules into cells Download PDFInfo
- Publication number
- WO2000044438A1 WO2000044438A1 PCT/US2000/000014 US0000014W WO0044438A1 WO 2000044438 A1 WO2000044438 A1 WO 2000044438A1 US 0000014 W US0000014 W US 0000014W WO 0044438 A1 WO0044438 A1 WO 0044438A1
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- WIPO (PCT)
- Prior art keywords
- electrodes
- electrode assembly
- electrode
- macromolecules
- molecules
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/18—Applying electric currents by contact electrodes
- A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
- A61N1/325—Applying electric currents by contact electrodes alternating or intermittent currents for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/18—Applying electric currents by contact electrodes
- A61N1/20—Applying electric currents by contact electrodes continuous direct currents
- A61N1/30—Apparatus for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body, or cataphoresis
- A61N1/303—Constructional details
- A61N1/306—Arrangements where at least part of the apparatus is introduced into the body
Definitions
- the present invention relates generally to methods and apparatus for delivery of macromolecules into cells. More specifically, the present invention provides methods and apparatus for delivering substances, such as macromolecules, e. g. polynucleotide vaccines (DNA vaccine and/or RNA vaccine) and protein-based vaccines, into selected cells in epidermal tissue with reduced sensation (reduced pain) .
- macromolecules e. g. polynucleotide vaccines (DNA vaccine and/or RNA vaccine) and protein-based vaccines
- the first DNA vaccination procedure in the prior art was called naked DNA vaccination because a liquid solution of DNA was injected into the muscle of mice with no additives to enhance transfection. This method does transfect a few cells and does induce an immune response to the expressed antigen in mice. However, in humans and primates, the method does not work well.
- an improvement in DNA vaccine efficiency was obtained by the use of a biolistic method for DNA delivery.
- the biolistic method is done by coating metal microbeads with DNA and shooting the particles into skin after accelerating the particles to a chosen velocity. This method works much better than naked DNA. Part of the reason is that the DNA coated particles are injected into the skin to a depth that increases the chance of transfecting Langerhans cells.
- the biolistic method has some disadvantages. First, it causes some skin damage that may scar in some individuals. Second, in spite of the increased efficiency, more efficiency is needed. Third, the ballistic particle remains inside the patient after treatment . In this respect, it would be desirable if a method for delivering DNA to biological cells were provided which does not cause skin damage that results in scarring. Also, it would be desirable if a method for delivering DNA to biological cells were provided which does not leave a residue of ballistic particles in cells that are treated. As a matter of interest, the following U. S. patents disclose biolistic methods: 5,036,006 and 5,478,744,
- U. S. Patent No. 5,019,034 of Weaver et al discloses a process for electroporation of tissues in which electrodes are placed on top of the tissue surface, such as skin, of a patient.
- Molecules that are used for treating the skin are placed in reservoirs on top of the skin surface, and the treatment molecules must penetrate into the skin tissues transdermally. That is, the treatment molecules must pass from outside the skin to inside the skin.
- the surface layer of the skin relatively impermeable, if the layers of the skin to be treated are near the basal lamina of the epidermis, then the treatment molecules must traverse considerable skin tissue before the cells to be treated are reached by the treatment molecules.
- Such a treatment method is inefficient for treating cells near the basal lamina.
- U. S. Patent No. 5,273,525 of Uoxmann discloses an apparatus for electroporation of drugs and genetic material into tissues which employs a hollow hypodermic needle for placing the drugs and genetic material in the vicinity of the tissues to be electroporated. Whenever a hollow hypodermic is employed in a tissue, the tissue is cut with a circular cut by the hollow hypodermic needle.
- U. S. Patent No. 5,318,514 of Hofmann discloses an applicator for the electroporation of drugs and genes into cells.
- the applicator includes a plurality of needle electrodes which can be penetrated into the skin of a patient.
- Material to be electroporated into the skin is retained in a fluid reservoir which wets an open cell foam elastomer carrier for the fluid. Because the material to be electroporated is retained in a fluid, in both the reservoir and the open cell foam elastomer, careful control of tlie amount of the .material -at -the -electrode surfaces is difficult.
- U. S. Patent No. 5,389,069 of Weaver discloses a method and apparatus for in vivo electroporation of tissues which employs a hollow cylindrical needle for providing treating substances deep into tissues. As mentioned above, avoiding the use hollow cylindrical needles would be desirable to avoid the pain involved therewith.
- U. S. Patent No. 5,697,901 of Eriksson discloses gene delivery into tissues by the use of a gene-carrying fluid medium that is pressurized in conjunction with hollow microneedles . Problems of control and flushing using fluid media have been discussed hereinabove. An electroporation step is not employed in the Eriksson patent. As a matter of interest, Eriksson addresses the subject of pain in two respects. There is a statement that the hollow microneedle system can be used for treating pain. There is a statement that pain in wounds can be relieved by cooling. It is noted by the present inventors herein that Eriksson does not discuss his treatment method per se as being of a pain free or reduced pain treatment method.
- the present inventors theorize that the pressurized fluid injection method that is employed by Eriksson is not conducive to a pain free or reduced pain treatment method.
- Still other features would be desirable in a method and apparatus for delivery of macromolecules into epidermal cells.
- the conductive base electrode portions and the conductive tips of the electrodes may exhibit electrical characteristics which are undesirable with respect to the electroporation process in general and the biological cells that are treated in particular.
- Electrodes assemblies having a plurality of needle electrodes have been employed on a patient, it may be a difficult task to clean and sterilize them for a subsequent use. In this respect, it would be desirable if a method and apparatus for delivery of macromolecules into cells were provided in which the electrode assemblies are disposable.
- a method for delivery of molecules into biological cells which includes the steps of:
- the pulse waveforms may be provided by applying a sequence of at least three single, operator-controlled, independently programmed, DC electrical pulses, to the biological cells.
- the sequence of at least three DC electrical pulses has one, two, or three of the following characteristics (a) at least two of the at least three pulses differ from each other in pulse amplitude, (b) at least two of the at least three pulses differ from each other in pulse width, and (c) a first pulse interval for a first set of two of the at least three pulses is different from a second pulse interval for a second set of two of the at least three pulses.
- the method can include a step of providing the electrode assembly holder with electrically conductive pathways between the electrode assembly and the waveform generator.
- the method can include a step of providing the electrode assembly in a sterile package. In such a case, the electrode assembly is removed from the sterile package prior to use. Further, the method can include the steps of providing the electrodes with electrically insulated outer surface electrode tip portions and electrically insulated outer surface electrode base portions .
- the molecules in the electrode coating can be in a solid phase.
- the molecules in the electrode coating are, preferably, macromolecules.
- the macromolecules in the electrode coating can include a polynucleotide vaccine (DNA vaccine and/or RNA vaccine) or a protein-based vaccine.
- the molecules can be delivered to Langerhans cells in epidermal tissue of a patient with reduced sensation (reduced pain or nearly painless or pain free) to the patient.
- the pulse waveforms have an absolute applied voltage in a range of 0.1 to 300 volts; (b) the electrodes of opposite polarity are separated by a separation distance in a range of from 50 to 500 microns; and (c) the electrodes are penetrated into the epidermal tissue a distance up to and slightly beyond the basal lamina layer of the epidermal tissue.
- the pulse waveforms which drive the coating molecules off of the electrodes are electrophoresis waveforms.
- the pulse waveforms which deliver the driven- off molecules into the biological cells are electroporation waveforms.
- common pulse waveforms both drive the coating molecules off of the electrodes and deliver the driven-off molecules into the biological cells.
- the biological cells can be in vivo, ex vivo, or in vitro. More specifically, the biological cells can be in epidermal tissue and can be Langerhans cells in the epidermal tissue.
- an apparatus for delivery of molecules into biological cells and includes a waveform generator which provides pulse waveforms.
- An electrode assembly holder is provided, and an electrode assembly is mechanically supported by the electrode assembly holder.
- the electrode assembly holder is also electrically connected to the waveform generator through electrically conductive pathways.
- the electrode assembly includes electrodes which are coated with the molecules to be delivered into the biological cells.
- the electrode assembly can be removable and replaceable from the electrode assembly holder.
- the electrode assembly includes electrode- assembly-conductive strips.
- the electrode assembly holder includes holder conductors which are registrable with the electrode-assembly-conductive strips when the electrode assembly is mechanically connected to the electrode assembly holder.
- the electrode assembly holder includes electrically conductive pathways between the holder conductors and the waveform generator.
- a sterile package can be provided for the electrode assembly.
- the sterile package is removed from the electrode assembly after the electrode assembly is mechanically supported by the electrode assembly holder and is electrically connected to the waveform generator.
- the waveform generator provides pulse waveforms which include a sequence of at least three single, operator-controlled, independently programmed, DC electrical pulses, to the biological cells.
- the sequence of at least three DC electrical pulses has one, two, or three of the following characteristics (a) at least two of the at least three pulses differ from each other in pulse amplitude, (b) at least two of the at least three pulses differ from each other in pulse width, and (c) a first pulse interval for a first set of two of the at least three pulses is different from a second pulse interval for a second set of two of the at least three pulses.
- the electrodes can include electrically insulated outer surface electrode tip portions and electrically insulated outer surface electrode base portions.
- the electrodes are coated with macromolecules, which may include a polynucleotide vaccine (a DNA vaccine and/or a RNA vaccine) and/or a protein-based vaccine.
- the polynucleotide vaccine or protein-based vaccine can be a solid form, coating the electrodes, prior to using the electrodes on a patient.
- a packaged sterile electrode assembly which includes a sterile electrode assembly which includes electrodes which are coated with the molecules to be delivered into biological cells.
- the electrode assembly includes electrode-assembly-conductive strips for connection to complementary electrically conductive pathways leading to the waveform generator.
- an internally sterile package encloses the sterile electrode assembly contained therein. With the packaged sterile electrode assembly, the electrodes can include electrically insulated outer surface electrode tip portions and electrically insulated outer surface electrode base portions.
- the electrodes are coated with macromolecules which can include a solid phase polynucleotide (DNA vaccine and/or RNA vaccine) and/or a solid phase protein-based vaccine.
- macromolecules which can include a solid phase polynucleotide (DNA vaccine and/or RNA vaccine) and/or a solid phase protein-based vaccine.
- transfection of cells with DNA in vivo, using electric field mediated transfection is an efficient process.
- electric fields can be used for the delivery of other macromolecules such as RNA and proteins into cells.
- the electric field delivery has one disadvantage, that being the pain induced by the high voltage electrical pulses required for the transfection.
- a method is provided for delivering macromolecules (DNA, RNA, and protein) to cells, in tissues in vivo, using painless (or nearly painless) and efficient electric field mediated delivery.
- the electric fields required for electric field mediated DNA transfection, by electroporation are in the range of 100 volts per cm to 20,000 volts per cm..
- this range is converted to a range of absolute voltages in a range of 5 to 1,000 volts.
- the range of absolute voltages is in a range of 0.1 to 1,000 volts.
- Pain sensation resulting from an electrical stimulus is dependent upon several factors. Among these are voltage, amperage, pulse rise time, pulse width, and pulse frequency. Above a threshold value applied directly to tissues in vivo, increased voltage or increased pulse width will result in an increase in pain.
- Two threshold values are important in painless in vivo electric field mediated delivery of macromolecules.
- a first threshold is the threshold for pain. It is desirable to reduce absolute voltage (and, if desired, also pulse width) applied directly to tissues in vivo below the threshold value for pain.
- an opposing second threshold is the minimal electric field required for electric field mediated delivery of macromolecules. Usually, increasing pulse parameters beyond this second threshold value results in increased efficiency in the delivery of macromolecules . An excessive increase will result in cell death.
- the important electric field parameter for electric field mediated delivery of macromolecules is volts per cm rather than absolute voltage.
- the absolute voltage needs to be decreased.
- the absolute voltage stays the same, the electric field increases.
- pulse width is also important. Very narrow pulse widths significantly increase the threshold voltage required for pain sensation. Electroporation efficiency, within certain limits, is proportional to the product of pulse voltage multiplied by the pulse width. This means that if voltage can be increased, the pulse width can be decreased. Therefore, a decrease in inter-electrode distance also will allow decreased pulse widths to be used.
- Another advantage of reduced inter-electrode distance is that fewer dermal nerves are located between the electrodes for the portions of the electrodes that may penetrate through the epidermis into the dermis. For a given applied voltage, the fewer nerves between the electrodes, the less pain is perceived. Another factor affecting pain is the proximity of the electric field to nerve endings. In the skin, nerves and nerve endings exist throughout the dermis but are absent in the epidermis. However, in the upper papillary layer of the dermis, nerves are relatively few and far between. This means that electrodes that only penetrate the epidermis or epidermis plus upper dermis (papillary layer of dermis) will not be near many nerves. Thus, very short electrodes would induce almost no pain upon insertion nor would pain be induced upon the application of an. electric field since the majority of the field is between the electrodes, which are located in the epidermis.
- a number of applications of the method and apparatus for delivery of macromolecules into cells, of the invention are contemplated. Briefly, such applications include polynucleotide vaccination, protein vaccination, and gene therapy.
- Proteins also can be introduced into cells using electric field mediated delivery.
- proteins are delivered outside cells using a hypodermic needle. This type of delivery is inefficient in inducing a cell mediated cytotoxic lymphocyte immune response.
- Some infectious diseases require a cytotoxic lymphocyte response as a component of the immune response for efficient clearance of the infection. Delivery of proteins into cells promotes the induction of that response.
- the method and apparatus of the present invention can be used to deliver therapeutic DNA into cells on the surface of any accessible organ in addition to the skin.
- the method of the invention is a method for painless, effective delivery of macromolecules to tissues, in vivo, for the purpose of vaccination (or treatment) , DNA vaccination, gene therapy, or other reasons.
- An electrode with at least one of two characteristics is used for delivery of macromolecules into cells in tissue.
- One of the two characteristics is an electrode length short enough that it does not penetrate to a depth in tissue with nerve endings.
- Another characteristic is that inter-electrode distances are small enough to allow pulse parameters (voltage and pulse width) to be used that are painless. Only one or the other of these characteristics is needed in any given application, however, they may be used together.
- Yet another object of the present invention is to provide a new and improved method and apparatus for delivery of macromolecules into cells that provides an electroporation method for delivering molecules to biological cells in the epidermis, near the basal lamina, without having the treatment molecules pass through the skin transdermally.
- Still a further object of the present invention is to provide a new and improved method and apparatus for delivery of macromolecules into cells which does not employ a hypodermic needle.
- Yet another object of the present invention is to provide a new and improved method and apparatus for delivery of macromolecules into cells that applies relatively low absolute voltage to cells undergoing electroporation.
- Still a further object of the present invention is to provide a new and improved method and apparatus for delivery of macromolecules into cells that can be used, if desired, to apply pulses having relatively short pulse width to the cells undergoing electroporation.
- Yet another object of the present invention is to provide a new and improved method and apparatus for delivery of macromolecules into cells which renders the base portions and tip portions of the electrodes nonconductive.
- Fig. 1 is a schematic illustration of the overall apparatus of the invention.
- Fig. 2 is a schematic illustration of the pre-coated electrodes of the invention penetrating an epidermal skin layer and being driven by pulse waveforms to deliver macromolecules into epidermal cells.
- Fig. 3 is a schematic illustration of tip portions of the electrodes .
- Fig. 4 is a side view of an electrode assembly.
- Fig. 5 is a bottom view of the electrode assembly shown in Fig. 4.
- Fig. 6 is a partial edge view of the electrode assembly shown in Fig. 5 illustrating the alternate polarity of alternate rows of electrodes.
- Fig. 7 is an enlarged top view of the electrode assembly shown in Fig. 4 which shows alternating conductors for contacting alternate rows of electrodes.
- Fig. 8 schematically shows an electrode assembly packaged in a sterile package.
- Fig. 9 schematically shows apparatus used for coating the electrodes with macromolecules.
- the method for delivery of molecules into biological cells employs the apparatus set forth and includes the steps of (a) coating electrodes 16 in an electrode assembly 12 with the molecules to be delivered, (b) attaching the electrode assembly 12 having coated electrodes 16 to an electrode assembly holder 13, (c) providing a waveform generator 15, (d) establishing electrically conductive pathways between the electrodes 16 and the waveform generator 15, (e) locating the electrodes 16 such that the biological cells are situated therebetween, and (f) providing pulse waveforms from the waveform generator 15 to the electrodes 16, such that molecules on the electrodes 16 are driven off of the electrodes 16 and delivered into the biological cells.
- the molecules are delivered with reduced sensation in a patient to Langerhans cells 22 in the epidermis 20 of a patient.
- the pulse waveforms have an absolute applied voltage in a range of from 0.1 to 300 volts. Electrodes 16 of opposite polarity are separated by a separation distance in a range of from 50 to 500 microns. The electrodes 16 are penetrated into the epidermal tissue up to and slightly beyond the basal lamina layer of the epidermal tissue.
- the pulse waveforms which drive the coating molecules off of the electrodes 16 are electrophoresis waveforms .
- the electrophoresis waveforms can be in a range of from 0.1 to 100 volts/cm..
- the pulse waveforms which deliver the driven-off molecules into the biological cells are electroporation waveforms.
- the electroporation waveforms can be in a range of from 100 to 10,000 volts/cm..
- Common pulse waveforms both drive the coating molecules off of the electrodes 16 and deliver the driven- off molecules into the biological cells.
- the biological cells to which the molecules are delivered can be in vivo, ex vivo, or in vitro. More specifically, the biological cells can be in the epidermis 20 (epidermal tissue) and can be Langerhans cells 22 in the epidermal tissue.
- the molecules driven off of the electrodes 16 by electrophoresis electrical pulses are delivered to the cells by electroporation pulses.
- the pulse waveforms are provided by the waveform generator 15 by applying a sequence of at least three single, operator-controlled, independently programmed, DC electrical pulses, to the biological cells.
- the sequence of at least three DC electrical pulses has one, two, or three of the following characteristics (1) at least two of the at least three pulses differ from each other in pulse amplitude, (2) at least two of the at least three pulses differ from each other in pulse width, and (3) a first pulse interval for a first set of two of the at least three pulses is different from a second pulse interval for a second set of two of the at least three pulses.
- the electrode assembly holder 13 is provided with electrically conductive pathways, which includes conductors 21, between the electrode assembly 12 and the waveform generator 15.
- the electrode assembly 12 can be provided in a sterile package 24 which is removed from the electrode assembly 12 prior to use.
- the electrodes 16 have conical tips, whereby they are referred to as needle electrodes .
- the electrodes 16 can be provided with electrically insulated outer surface electrode tip portions 17 and electrically insulated outer surface electrode base portions 19.
- the electrically insulated outer surface electrode base portions 19 minimize current flowing across the upper skin surface when the pulse voltage is applied.
- DNA binds poorly to the electrically insulated outer surface electrode base portions 19.
- the outer surface area of the electrodes 16 between the electrically insulated outer surface electrode tip portions 17 and the electrically insulated outer surface electrode base portions 19 does not have an outer insulation layer and is a good surface for DNA binding.
- the outer surface areas between the electrically insulated outer surface electrode tip portions 17 and the electrically insulated outer surface electrode base portions 19 is conductive and can be referred to as an active electrode area.
- the electrically insulated outer surface electrode tip portions 17 prevent large local electric field intensity which may cause burning in the tissue.
- An electrode assembly 12 that is suitable for delivering DNA vaccines to Langerhans cells 22 in the epidermis 20 of the forearm has the following characteristics : (a) electrode length -- 130 microns
- the lengths of the electrodes 16 are determined by the thickness of the epidermis 20.
- the thickness of the epidermis 20 varies in different parts of the human body. For example, the thickness of the epidermis 20 on the medial forearm or the lateral upper arm above the deltoid muscle is considerably thinner than the thickness of the epidermis 20 on the heel or sole of the foot.
- the molecules in the electrode coating are in a solid phase and are preferably macromolecules 18.
- the macromolecules 18 in the electrode coating can include a DNA vaccine and/or a protein-based vaccine.
- the DNA vaccine and the protein-based vaccine can be in the form of a solid phase DNA vaccine or protein-based vaccine applied to the electrodes 16.
- the electrode assembly 12 is removable and replaceable from the electrode assembly holder 13.
- the electrode assembly 12 includes electrode-assembly- conductive strips 26.
- the electrode assembly holder 13 includes holder conductors which are registrable with the electrode-assembly-conductive strips 26 when the electrode assembly 12 is mechanically connected to the electrode assembly holder 13.
- the electrode assembly holder 13 includes electrically conductive pathways between the holder conductors and the waveform generator 15.
- the waveform generator supplies the electrical pulses necessary for generating the electric field in the tissue.
- the electrode assembly 12 contains the electrodes 16, and the DNA or protein macromolecules are applied to the electrodes 16.
- the electrode assembly holder 13 connects the electrode assembly 12 to the waveform generator 15.
- the electrode assembly 12 can be in the form of an electrode array can be in the form of a disposable, onetime-use electrode array which has the macromolecules preloaded onto the electrodes. In this respect, the preloaded electrode array can be provided as a sterile package.
- the sterile package is opened, and the electrode array is connected to the electrode array holder.
- the electrode assembly holder is grasped by a person, the electrode assembly is pressed upon the skin of a patient, and the electrode assembly is pressed into the skin of the patient so that the electrode assembly penetrates the stratum corneum of the epidermis.
- the tips of the electrodes in the electrode assembly are located in the region of the Langerhans cells, which are dendritic cells of the epidermis.
- an electrode assembly 12 includes a non-conductive electrode carrier 14 and a plurality of individual needle electrodes 16 supported by the electrode carrier 14.
- the active areas 29 of the electrodes 16 are coated with macromolecules which are illustrated as small "x's" 18 on the surfaces of the electrodes 16.
- some of the macromolecules 18 are driven off of the electrodes 16 by electrophoresis voltage and enter the epidermis 20 and are delivered to the dendritic Langerhans cells 22 and the living epithelial cells 23 in the living epidermis above the basal lamina 25 in the epidermis 20 by electroporation voltage.
- the waveform generator 15 produces the pulses for the protocol .
- the output of the waveform generator can be conventional with a single selection of pulse parameters such as voltage, pulse width, interval between pulses, number of pulses and the vector of the pulse.
- the output of the waveform generator can be programmable with the ability to change any of the parameters (voltage, pulse width, interval between pulses, number of pulses,) from pulse to pulse.
- the variable output is needed for optimal performance because a different electric field is required for macromolecular movement off of the electrodes 16 than the electric field required for electric field mediated delivery of macromolecules into cells.
- a suitable programmable pulse generator is the PulseAgile (Registered in U. S.
- Model PA-4000 Electroporation System made by Cyto Pulse Sciences, Inc., P. 0. Box 609, Columbia, MD 21045. It is noted that the Model PA-4000 delivers rectangular waves of various amplitudes (voltages), width, and intervals.
- programmable control of two other parameters is desired.
- One is control of the direction or vector of the applied electric field.
- the other is control of electrode selection.
- electric field direction could be reversed to insure better distribution of the macromolecule.
- individual pairs of electrode arrays could be sequentially selected.
- a suitable device for electrode selection and the selection of electrode field direction is the programmable pulse switch, which is an optional component of the above- mentioned PA-4000 Electroporation System.
- the electrode assembly 12 serves two functions . It delivers the macromolecule to the desired site and it delivers the electric field to the tissue.
- the electrode assembly 12 consists of: 1. a non-conductive electrode carrier 14. 2. an array of needle electrodes 16 fabricated on the electrode carrier 14.
- Electrode-assembly-conductive strips 26 for electrical connection to the holder conductors on the electrode assembly holder 13 to connect electrically to the waveform generator 15.
- the tips of the needle electrodes 16 are pressed against the epidermis 20 of a patient with the needles puncturing the stratum corneum 27 and extending into the epidermis 20 and the upper dermis 31 as shown in Fig. 2.
- the needle array consists of N rows that are conductive. Each needle electrode 16 in a row is connected to all other needles in that row electrically. The rows are be driven by electrical pulses, either: 1. independently 2. in pairs
- the electrode assemblies (arrays) needed for electric field mediated macromolecular delivery (for DNA transfection and protein delivery) to Langerhans cells 22 in the epidermis 20 have the following specifications: 1. Multiple conductive rows per array, each needle electrode in a conductive row being electrically connected together. 2. Adjacent rows are electrically isolated from each other.
- Needle length is selected to conform to epidermal thickness in area treated.
- Distance between the nearest electrically linked electrode needles being selected to minimize tissue injury and to maximize electric field.
- Electrodes can be electrically addressed either individually, in rows, or in groups of rows.
- the electrode needles can have many shapes .
- needle electrode shapes are: cylindrical needles, flat needles, cone shaped needles, and blade needles.
- the needles can be pointed rounded or blunt. Each of these shapes can be single or multiple per electrode row
- the purposes of the electrode assembly holder are to establish an electrical connection between the waveform generator and the electrode assembly and to provide a support for the electrode assembly when the electrode assembly is applied to the patient. It provides a mechanical connection for application to the patient. It also provides a means of delivering the electrode assembly to the patient ' s tissue while maintaining sterility of the electrode assembly.
- the electrode assembly can have the following optional features . It can have a means to provide proper pressure on the electrode assembly to the tissue. It can have indicators that indicate correct application pressure, on-going electrical delivery and completion of electrical delivery. It can have a switch for initiation of the pulse protocol. It can have a means for automatically initiating a pulse protocol when proper pressure is applied to the electrode assembly holder.
- macromolecules including DNA and protein macromolecules
- electrophoresis voltages so that they can move through the extra-cellular spaces of tissue prior to the application of electroporation pulses for delivering the macromolecules into the targeted biological cells in the tissue.
- the macromolecules are initially bound to the external surfaces of the electrodes 16.
- a relatively high concentration of macromolecules 18 is dissolved or suspended in a solvent or liquid carrier.
- the electrodes 16 are then dipped into the solution or suspension. Then, the solvent or liquid carrier is evaporated, leaving a solid coating of macromolecules 18 on the electrodes 16.
- the electrodes 16 are coated by spraying. Other mechanical means of coating the electrodes 16 are possible.
- Macromolecules such as DNA bind with good efficiency to many surfaces.
- the physical and chemical properties of the material can be used to enhance binding to electrode surfaces .
- Molecules tend to bind to each other through various molecular interactions, each having a different binding strength. These same forces are active between solid substrates and soluble molecules as well as among molecules in solution.
- the molecular interactions are:
- Solvation Solvent binding. An interaction between the components of a molecule and the solvent molecules.
- Hydrophobic interaction A solute-solute interaction as a consequence of the inability to interact with the solvent; an avoidance interaction
- Van der Walls forces are weak attractions that exist between all molecules. It is effective only at short distances and can be stronger if interactions based upon complementary shape
- Hydrogen bonds are bonds formed between hydrogen and other molecules such as nitrogen and oxygen.
- DNA is both sparingly soluble in water and charged.
- the organic rings within the nucleotides impart the hydrophobic properties to DNA.
- the phosphate molecules in the DNA polymer impart a net negative charge.
- the strongest bond between an electrode surface and DNA is the hydrophobic bond.
- DNA moves towards the electrode thereby enhancing the interaction of DNA with the conductive hydrophobic surface.
- the electrical charge will be reversed. Migration from the electrode surface occurs as soon as the repelling force of like charges exceeds the force of the hydrophobic and other molecular interaction.
- DNA can be coated onto specific sites by binding the DNA to metal (such as an electrode surface) or another conductive material through the use of a positive charge. Subsequently, for driving the DNA off of the electrode surface and for subsequently delivering the DNA to biological cells, a negative charge is applied to the same surface. DNA, being negatively charged, will migrate in an electric field toward the positive electrode. This phenomenon is called electrophoresis. If the positive electrode is a hydrophobic surface as are most metals, the positive charge and the hydrophobic interaction will work together to hold the DNA to the surface.
- DNA for instance, is negatively charged at physiological pH. This means that a DNA molecule will migrate towards a positive electrode. This property is used to bring the macromolecule in contact with the electrode where binding occurs via the other molecular interactions listed. DNA, for instance, can bind because it is hydrophobic.
- DNA is negatively charged and therefore migrates to a positively charged electrode.
- Fig. 9 which illustrates an apparatus used for coating the electrodes 16
- DNA is added to a buffer solution and then placed into a chamber with an electrode that serves as the cathode.
- this electrode is separated from the buffer by a gel interface to prevent metal of the cathode coming into contact with the DNA.
- the electrode device is inserted into the liquid, and a positive charge is applied to the electrode device drawing the DNA to the surface of the electrode device.
- the DNA attaches to the surface of the electrode device by hydrophobic or other interaction until expelled by a reverse charge.
- the DNA is dried on to the device with or without a protectant, such as sugars, and with or without other carrier molecules. Substances can also be added to the coating on the electrodes which promote uptake of the treating material into the target cells.
- the amount of macromolecule on the electrode assembly varies depending upon the application.
- the electrode assembly is loaded with 0.01 to 100 micrograms of plasmid DNA.
- Sterile materials and a sterile local environment can be used in the manufacture of the electrode assembly with the macromolecule. Alternatively, the assembly can be sterilized after manufacture.
- a typical sequence of steps in administering macromolecules 18 to a patient using the method and apparatus of the invention are described as follows.
- the waveform generator 15 would be connected to the electrode assembly holder 13.
- an electrode assembly 12 whose electrodes 16 have been loaded with the desired macromolecule is selected.
- the electrode assembly 12 is then mechanically connected to the electrode assembly holder 13.
- the electrodes 16 are pressed onto the patient's tissue (typically skin) .
- the electrodes 16 penetrate into the epidermis 20 and extend substantially only to the basal lamina layer.
- the macromolecular delivery process is started, and the selected pattern of electric fields is initiated. After completion of the delivery protocol, the electrodes 16 are removed from the epidermis 20, and the electrode assembly 12 is discarded.
- the electrical protocol is designed to drive the macromolecules off of the electrodes 16 into the tissue, followed by delivery of the macromolecules into cells in the tissue.
- a typical sequence of electrical pulses is as follows. First, a series of low voltage (electrophoresis) pulses are applied to the electrodes 16 to remove the DNA from all negatively charged electrodes. Typically, alternating rows of electrodes are negatively charged. Next, higher voltage electroporation pulses are applied to the electrodes 16 to drive the DNA into cells. Next, electrode polarity is reversed and low voltage pulses delivered with opposite polarity to remove the DNA from the remaining electrodes. Higher voltage electroporation pulses are then applied to force the DNA into cells.
- low voltage (electrophoresis) pulses are applied to the electrodes 16 to remove the DNA from all negatively charged electrodes. Typically, alternating rows of electrodes are negatively charged.
- higher voltage electroporation pulses are applied to the electrodes 16 to drive the DNA into cells.
- electrode polarity is reversed and low voltage pulses delivered with opposite
- a significant use of this macromolecular delivery system is to deliver macromolecules to skin.
- electrode needle length is chosen to allow penetration of the electrode to the stratum basalis and basement membrane (basal lamina) . Some slight penetration into the dermis may occur.
- an electrode length of 130 microns is selected. This depth allows treatment of cells of the epidermis.
- the cells transfected by this delivery method are dendritic cells (skin Langerhans cells 22) and epithelial cells.
- the method and apparatus of the invention can be used in other biological environments, such as tissues during surgery and with plants.
- Standard microchip manufacturing processes can be adapted to make the conductive microneedles on a non- conductive support, as in accordance with the invention.
- a blank consisting of a silicon or other non conductive layer and a metal layer would be used.
- the mask would be designed to encourage more etching between rows than within rows, resulting in conductive rows of electrodes with nonconductive spaces between rows.
- Electrode material and adjacent insulating material are prepared by mixing a ceramic, metal or other powder with a thermoplastic binder.
- the individual components are assembled and warm pressed to stick together.
- the resulting rod is extruded to reduce its size.
- the new rods are assembled in a rod composed of a multiple of the extruded rod.
- This newest rod is re-extruded to reduce the size of the multiple rods to the size of the first extruded rod.
- the parts can be heated to remove the binder.
- a second, higher heat is used to sinter the metal or ceramic powders together.
- the rods are cut into disks before or after the sintering. Differential sand blasting or other mechanical or chemical techniques can be used to raise the needles above the surface of the insulator.
- Another method for manufacture would be to use laser milling techniques to remove material from a sandwich composed of conductive and nonconductive layers.
- the distance between the electrodes is large enough for mechanical assembly.
- An example of such assembly follows. Wire of the desired metal composition and diameter is arranged on spools for assembly. The wires are fed into an apparatus that aligns the wire to the correct distance apart. Ceramic or plastic material is injected into a flow through system that results in complete filling of the gap between the electrodes and forms the shape of the outside rim of the electrode. The plastic or ceramic is hardened and cut into discs. The resulting disks are differentially eroded, taking advantage of the softer matrix. The erosion can be done using mechanical methods, chemical methods or a combination of methods . The surface erosion leaves needles of the desired length protruding above the supporting matrix.
- Stainless steel needles 30 mm in length and 120 microns in diameter are obtained.
- One source is from an acupuncture supply company. Seirin No. 02 needles are an example.
- the needles are cut from the handle if one is present.
- a number of needles are selected for each row of the device. Thirty-five needles per row are used for this example.
- the needles are carefully placed side by side with the tips of the needles in line. This step requires care and a jig made of a microscope slide glued at 90 degrees on top of another microscope slide is a tool to help in the alignment.
- the slide also is be used to check the alignment on a microscope.
- the needle row (needle bundle) is taped together with 50 micron thick tape.
- Two or more of the needle bundles are stacked to form an electrode array with the tips of each bundle aligned with the next bundle.
- the needles are silver soldered to a wire, and alternating needle bundles are connected together electrically.
- An overall support structure is provided to support the electrode array of needle bundles .
- the present invention accomplishes all of the objects set forth by providing a method and an apparatus for delivery of macromolecules into cells that do not cause skin damage that results in scarring.
- a method and an apparatus for delivery of macromolecules into cells are provided which do not leave a residue of ballistic particles in cells that are treated.
- an electroporation method for delivering molecules to biological cells in the epidermis, near the basal lamina does not have the treatment molecules pass through the skin transdermally.
- a method and an apparatus for delivery of macromolecules into cells are provided which do not employ a hypodermic needle .
- a method and an apparatus for delivery of macromolecules into cells are provided which do not employ a fluid medium that flows down onto the electrodes as the electroporation process is being carried out on the patient.
- a method and an apparatus for delivery of macromolecules into cells which do not employ a pressurized fluid injection step for injecting fluid into a patient.
- relatively low absolute voltages are applied to cells undergoing electroporation.
- pulses that are applied to the cells can have, if desired, relatively short pulse width to the cells undergoing electroporation.
- a method and an apparatus for delivery of macromolecules into cells are provided which can employ, if desired, electrodes in which the base portions and tip portions of the electrodes are nonconductive.
- a method and an apparatus for delivery of macromolecules into cells provide disposable electrode assemblies .
- a method and an apparatus for delivery of macromolecules into cells are provided in which electrode assemblies are packaged in sterile packaging.
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Abstract
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Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00903076A EP1163024B1 (en) | 1999-01-28 | 2000-01-12 | Delivery of macromolecules into cells |
US09/889,581 US6603998B1 (en) | 1999-01-28 | 2000-01-12 | Delivery of macromolecules into cells |
CA002372409A CA2372409C (en) | 1999-01-28 | 2000-01-12 | Delivery of macromolecules into cells |
AT00903076T ATE256484T1 (en) | 1999-01-28 | 2000-01-12 | INTRODUCTION OF MACROMOLECULES INTO CELLS |
JP2000595738A JP4499295B2 (en) | 1999-01-28 | 2000-01-12 | Delivery of macromolecules into cells |
DE60007290T DE60007290T2 (en) | 1999-01-28 | 2000-01-12 | INTRODUCTION OF MACROMOLECULES IN CELLS |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11775599P | 1999-01-28 | 1999-01-28 | |
US60/117,755 | 1999-01-28 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
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US09/889,581 A-371-Of-International US6603998B1 (en) | 1999-01-28 | 2000-01-12 | Delivery of macromolecules into cells |
US09/920,861 Continuation-In-Part US6713291B2 (en) | 1999-01-28 | 2001-08-03 | Electrodes coated with treating agent and uses thereof |
Publications (1)
Publication Number | Publication Date |
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WO2000044438A1 true WO2000044438A1 (en) | 2000-08-03 |
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ID=22374647
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PCT/US2000/000014 WO2000044438A1 (en) | 1999-01-28 | 2000-01-12 | Delivery of macromolecules into cells |
Country Status (8)
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US (1) | US6603998B1 (en) |
EP (1) | EP1163024B1 (en) |
JP (1) | JP4499295B2 (en) |
CN (1) | CN1191872C (en) |
AT (1) | ATE256484T1 (en) |
CA (1) | CA2372409C (en) |
DE (1) | DE60007290T2 (en) |
WO (1) | WO2000044438A1 (en) |
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- 2000-01-12 CA CA002372409A patent/CA2372409C/en not_active Expired - Lifetime
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- 2000-01-12 CN CNB008054509A patent/CN1191872C/en not_active Expired - Lifetime
- 2000-01-12 US US09/889,581 patent/US6603998B1/en not_active Expired - Lifetime
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Also Published As
Publication number | Publication date |
---|---|
ATE256484T1 (en) | 2004-01-15 |
CA2372409A1 (en) | 2000-08-03 |
JP2002535100A (en) | 2002-10-22 |
DE60007290T2 (en) | 2004-09-23 |
CN1191872C (en) | 2005-03-09 |
JP4499295B2 (en) | 2010-07-07 |
EP1163024B1 (en) | 2003-12-17 |
EP1163024A1 (en) | 2001-12-19 |
US6603998B1 (en) | 2003-08-05 |
CA2372409C (en) | 2005-03-29 |
EP1163024A4 (en) | 2002-10-24 |
CN1346290A (en) | 2002-04-24 |
DE60007290D1 (en) | 2004-01-29 |
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