WO2000043505A3 - Protease with two aspartate radicals in the catalytically active structure - Google Patents

Protease with two aspartate radicals in the catalytically active structure Download PDF

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Publication number
WO2000043505A3
WO2000043505A3 PCT/EP2000/000390 EP0000390W WO0043505A3 WO 2000043505 A3 WO2000043505 A3 WO 2000043505A3 EP 0000390 W EP0000390 W EP 0000390W WO 0043505 A3 WO0043505 A3 WO 0043505A3
Authority
WO
WIPO (PCT)
Prior art keywords
aspartate
protease
radicals
catalytically active
active structure
Prior art date
Application number
PCT/EP2000/000390
Other languages
German (de)
French (fr)
Other versions
WO2000043505A2 (en
Inventor
Kay Hofmann
Original Assignee
Memorec Medical Molecular Res
Kay Hofmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19902550A external-priority patent/DE19902550A1/en
Application filed by Memorec Medical Molecular Res, Kay Hofmann filed Critical Memorec Medical Molecular Res
Priority to CA002360585A priority Critical patent/CA2360585A1/en
Priority to AU25421/00A priority patent/AU2542100A/en
Priority to EP00903587A priority patent/EP1144603A3/en
Publication of WO2000043505A2 publication Critical patent/WO2000043505A2/en
Publication of WO2000043505A3 publication Critical patent/WO2000043505A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Catalysts (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to protease with two aspartate radicals in a catalytically active structure, a first aspartate radical being in a sequence X1GX2GD and a second aspartate radical being in a sequence X3X4DX5, X1, X2, X3 and X5 being selected independently of each other from the following: Ala, Val, Leu, Met and Ile; and X4 being an aromatic amino acid. The sequences X1GX2GD and X3X4DX5 lie in a transmembrane region.
PCT/EP2000/000390 1999-01-22 2000-01-19 Protease with two aspartate radicals in the catalytically active structure WO2000043505A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002360585A CA2360585A1 (en) 1999-01-22 2000-01-19 Protease
AU25421/00A AU2542100A (en) 1999-01-22 2000-01-19 Protease
EP00903587A EP1144603A3 (en) 1999-01-22 2000-01-19 Protease

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE19902550A DE19902550A1 (en) 1999-01-22 1999-01-22 A novel aspartate protease, coding sequence, inhibitors and antibodies, useful for treatment and diagnosis of Alzheimer's disease
DE19902550.9 1999-01-22
DE19925946 1999-06-08
DE19925946.1 1999-06-08
DE19929115.2 1999-06-24
DE19929115 1999-06-24

Publications (2)

Publication Number Publication Date
WO2000043505A2 WO2000043505A2 (en) 2000-07-27
WO2000043505A3 true WO2000043505A3 (en) 2001-11-29

Family

ID=27218935

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/000390 WO2000043505A2 (en) 1999-01-22 2000-01-19 Protease with two aspartate radicals in the catalytically active structure

Country Status (4)

Country Link
EP (1) EP1144603A3 (en)
AU (1) AU2542100A (en)
CA (1) CA2360585A1 (en)
WO (1) WO2000043505A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8609087B2 (en) 1999-07-12 2013-12-17 Trustees Of Dartmouth College Compounds and methods for identifying compounds which inhibit a new class of aspartyl proteases
US6887677B1 (en) 1999-07-12 2005-05-03 Trustees Of Dartmouth College Compounds and methods for identifying compounds which inhibit a new class of aspartyl proteases
GB0126782D0 (en) * 2001-11-07 2002-01-02 Medical Res Council Assay

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998033916A2 (en) * 1997-01-31 1998-08-06 Genetics Institute, Inc. Secreted proteins and polynucleotides encoding them
WO1998040404A2 (en) * 1997-03-11 1998-09-17 Genetics Institute, Inc. Secreted proteins and polynucleotides encoding them
WO1999003990A1 (en) * 1997-07-16 1999-01-28 Human Genome Sciences, Inc. 64 human secreted proteins
WO1999033873A1 (en) * 1997-12-26 1999-07-08 Ono Pharmaceutical Co. Ltd. NOVEL POLYPEPTIDES, cDNAS ENCODING THE SAME AND UTILIZATION THEREOF
WO1999043693A1 (en) * 1998-02-26 1999-09-02 Human Genome Sciences, Inc. 36 human secreted proteins
WO2000017222A1 (en) * 1998-09-23 2000-03-30 Human Genome Sciences, Inc. 31 human secreted proteins

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998033916A2 (en) * 1997-01-31 1998-08-06 Genetics Institute, Inc. Secreted proteins and polynucleotides encoding them
WO1998040404A2 (en) * 1997-03-11 1998-09-17 Genetics Institute, Inc. Secreted proteins and polynucleotides encoding them
WO1999003990A1 (en) * 1997-07-16 1999-01-28 Human Genome Sciences, Inc. 64 human secreted proteins
WO1999033873A1 (en) * 1997-12-26 1999-07-08 Ono Pharmaceutical Co. Ltd. NOVEL POLYPEPTIDES, cDNAS ENCODING THE SAME AND UTILIZATION THEREOF
EP1043333A1 (en) * 1997-12-26 2000-10-11 Ono Pharmaceutical Co., Ltd. NOVEL POLYPEPTIDES, cDNAS ENCODING THE SAME AND UTILIZATION THEREOF
WO1999043693A1 (en) * 1998-02-26 1999-09-02 Human Genome Sciences, Inc. 36 human secreted proteins
WO2000017222A1 (en) * 1998-09-23 2000-03-30 Human Genome Sciences, Inc. 31 human secreted proteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GENEMBL 1 August 1998 (1998-08-01), LAMERDIN ET AL: "FOS39554_1", XP002141740 *
DATABASE GENEMBL 8 August 1996 (1996-08-08), HILLIER ET AL.: "zc51b02.r1 Soares senescentr fibroblasts NbHSF Homo sapiens cDNA clone IMAGE:325803", XP002157596 *

Also Published As

Publication number Publication date
EP1144603A3 (en) 2002-02-06
EP1144603A2 (en) 2001-10-17
AU2542100A (en) 2000-08-07
WO2000043505A2 (en) 2000-07-27
CA2360585A1 (en) 2000-07-27

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