WO2000043199A1 - Magnetically responsive gel particle - Google Patents
Magnetically responsive gel particle Download PDFInfo
- Publication number
- WO2000043199A1 WO2000043199A1 PCT/US2000/001311 US0001311W WO0043199A1 WO 2000043199 A1 WO2000043199 A1 WO 2000043199A1 US 0001311 W US0001311 W US 0001311W WO 0043199 A1 WO0043199 A1 WO 0043199A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- gel
- particle
- scavenger
- nanometers
- magnetically responsive
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
- B01J20/28047—Gels
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
- B01J20/06—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising oxides or hydroxides of metals not provided for in group B01J20/04
- B01J20/08—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising oxides or hydroxides of metals not provided for in group B01J20/04 comprising aluminium oxide or hydroxide; comprising bauxite
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
- B01J20/10—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
- B01J20/103—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate comprising silica
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28009—Magnetic properties
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
- B01J20/28016—Particle form
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
- B01J20/28069—Pore volume, e.g. total pore volume, mesopore volume, micropore volume
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/5434—Magnetic particles using magnetic particle immunoreagent carriers which constitute new materials per se
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/90—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving iron binding capacity of blood
Definitions
- the invention relates to gel particles.
- iron In humans the metabolic requirements for iron can be determined by the rate of absorption which is matched by a normally fixed rate of loss. A state of iron deficiency can occur if dietary intake of iron is insufficient, if iron abso ⁇ tion by mucosal membranes is impaired, or if excessive bleeding occurs. Severe cases may lead to iron deficiency anemia. Conversely, genetic defects in the abso ⁇ tion of iron can result in an overload of iron which can lead to chronic liver disease and premature death. Iron is stored as a component of the protein ferritin and as part of insoluble hemosiderin. Iron is transported from the abso ⁇ tion and storage sites to the sites of use via specific iron-binding proteins — most notably transferrin, which is usually one-third saturated with iron.
- TIBC total iron-binding capacity
- the typical absorants/adsorbants that have been used to remove excess ferric ion, without stripping transferrin-bound iron include magnesium carbonate, amberlite resin, and activated alumina. These all usually require centrifugation, which yields a supernate that contains the protein-bound iron for analysis.
- N popular modification uses dry alumina columns for adso ⁇ tion. In this format, the diluted serum, containing excess iron, is poured onto the column and the eluate is collected for TIBC analysis.
- the invention features a gel particle that includes a scavenger, a magnetically responsive particle (MRP) and a gel.
- the scavenger can be, for example, a cell, a receptor, charcoal, silica or alumina.
- the scavenger and the MRP are disposed within the gel.
- a scavenger is a particle that can have an analyte (e.g., DNN, a polynucleotide, a protein, a metalloprotein, a metal ion, or a nucleoside acid, such as cyclic AMP, cyclic GMP or their intracellular messengers) attach thereto.
- analyte e.g., DNN, a polynucleotide, a protein, a metalloprotein, a metal ion, or a nucleoside acid, such as cyclic AMP, cyclic GMP or their intracellular messengers
- examples of scavengers include a cell, a receptor, charcoal, silica and alumina.
- N gel particle is a particle that includes a gel having a scavenger and an MRP disposed within the gel (e.g., the scavenger and the MRP are encapsulated in the gel, which is a shape-retaining gel
- the scavenger may or may not be attached (e.g., chemically bound) to the MRP.
- examples of gels include polyacrylamide, agarose and alginate. Gels can be gelled or cross-linked.
- Gel particles can be formed by adding a scavenger and an MRP to a solution of liquefied gel (e.g., sodium alginate) to form a suspension, and then expanding the suspension through an air jet. Gel particles can also be formed using emulsification, dripping and Rayleigh jet. Methods of preparing a gel particle containing a cell are disclosed in, for example, U.S. Patents No. 4,701,326; 5,567,451; and 5,846,530.
- the invention features a gel particle that includes a scavenger, an MRP and a gel.
- the scavenger and the MRP are disposed within the gel.
- the gel should have a porosity that allows one or more desired analytes through while not allowing undesired analytes through.
- the particular undesired analytes will vary depending upon the desired analyte(s). Examples of desired analytes include DNA, a protein, a metalloprotein or a metal ion. Examples of undesired analytes include cellular debris, cell walls, proteins or antibodies.
- a desired analyte can be a metal ion (e.g., iron ion), and an undesired analyte can be the desired analyte bound to a protein (e.g., a metal ion, such as an iron ion, bound to a protein, such as transferrin).
- a desired analyte is iron ion
- an undesired analyte is iron-bound transferrin.
- the porosity of gel can be, for example, about 1-1000 nanometers (mn) (e.g., about 20-200 mn, or about 200-500 nm).
- the invention features a sample (e.g., from a patient) interacting with one of these gel particles.
- the invention features a method of removing an analyte from a sample.
- the analyte can be, for example, a protein, DNA, a metal ion, a metalloprotein, a steroid, or a metal-containing analyte.
- the method includes contacting the sample with a gel particle that a magnetically responsive particle.
- the method also includes removing the analyte from the sample.
- TIBC methods of the invention can (a) eliminate centrifugation, (b) be rapid, (c) use small amounts of serum (e.g,. less than about 500 uL), and/or (d) can be adapted to on-line automation or semi-automation.
- Fig. 1 is a graph of a regression analysis of a correlation study
- Fig. 2 is a graph of a regression analysis of a linearity study.
- the preferred sample is serum, but plasma can also be used.
- Suitable sample sizes can be less than 1,000 microliters (e.g., less than about 500 microliters, such as about 200 microliters or 100 microliters). They can be 10-500 microliters (e.g., 5-10 microliters, or 50-75 microliters).
- the sample can be from a patient (e.g., a human patient) or from a culture (e.g., tissue culture or fermentation process. Sequestration
- Embodiments of the invention can allow, for example, the determination of TIBC in a sample, without a centrifugation step.
- the free iron in a sample can be sequestered by contacting the sample with a gel particle containing a scavenger (e.g., alumina) and an MRP.
- a scavenger e.g., alumina
- the gel particle can serve to separate the sample into a TIBC phase and a phase which includes sequestered Fe.
- the partition into phases can be effected without centrifugation and/or without removing either phase from the container which holds the sample.
- Scavengers should be capable of binding an analyte of interest.
- Scavengers include cell receptors, ion absorbers (e.g., alumina, silica or charcoal), metal complexing agents (e.g., crown ether, and dendrimers) and ion exchange resins.
- Other scavengers include polyacrylic acid, deferoxamine and other iron-binding iminocarboxylic acids (e.g., EDTN, EGTA, ⁇ TA and/or HEDTN), and other iron- binding carboxylic acids (e.g., tartaric acid, malonic acid, succinic acid, citric acid and/or tricarballic acid).
- the scavenger does not need to be specific for iron as long as it absorbs Fe "1-1" and/or Fe 4" ⁇ .
- the scavenger can be neutral grade alumina, such as commercially available from Scientific Adsorbents, Inc. (Atlanta, GA), J.T. Baker (Phillipsburg, NJ), or Sigma Chemical Co. (St. Louis, MO).
- Magnetically Responsive Particles (MRP's) MRP's are particles which respond to a magnetic field.
- MRP's which include an iron binding ligand can be included in the gel particle.
- the free iron passes through the gel, and is bound to the MRP's.
- a magnetic field is applied to the sample to partition the gel particles (e.g., by bringing them to the bottom of the sample container) and to form a phase which is free of MRP's, the TIBC phase.
- the iron in the TIBC phase (e.g., supernatant phase) is then measured.
- An MRP can be made by the entrapment or precipitation of iron, nickel or cobalt salts in the particle rendering the particle paramagnetic.
- the particles should not release or leach free iron.
- a suitable example is a particle made from styrene co- polymerized with acrylic acid in the presence of an iron, nickel or cobalt salt which are encapsulated with an additional co-polymerized surface layer. Similar particles are available commercially as ESTAPOR ® supe ⁇ aramagnetic particles.
- Another suitable example is a particle is polyacrolein polymerized with precipitated metal salts (e.g., iron salts, such as magnetite), and then ground to size.
- Such particles are commercially available from, for example, Cortex Biochem, Inc., under the trademark MagAcrolein .
- Other suitable particles are commercially available from Polysciences, Inc. (Warrington, PN, trademark PolyMag ® and Bangs Laboratories (Fishers, IN).
- suitable particles are those which are the product of the polymerization of monomers such as methacrylate, divinylbenzene, acrylamide, acrylic acid, or polyethylene in various combinations and proportions. Polymerization should be performed in the presence of iron, nickel or cobalt salts and an additional polymerization step should be used to encapsulate the particles.
- MRP's include particles of cellulose, agarose (e.g., type IN agarose, commercially available from Sigma Chemical Co., located in St. Louis, MO, or commercially available from FMC Bioproducts, located in Rockland, ME), or other polysaccharides with an iron, nickel or cobalt salt precipitated within the particle. These particles can be washed in strong acid to remove surface iron, nickel or cobalt which could leach from the particle. An example of such a particle is sold by Cortex under the name MAGCELL ® .
- Iron, nickel or cobalt can also be covalently attached to a particle.
- Iron chelating polymers can be used as iron binding ligands in methods of the invention.
- U.S. Patents No. 5,487,888 and 3,899,472 describe a variety of chelating polymers. These polymers can be used to encapsulate magnetic particles for use in methods described herein.
- the particle is preferably less than about 1 ,000 microns in diameter, and more preferably less than about 10 microns, or less than about 1 micron in diameter. In some embodiments, the particle is about 1 micron in diameter.
- Iron in a sample can be measured (e.g., with a colorometric method).
- the sample can be reacted with a dye (e.g., ferene or ferrozine, or other pyridyl or phenanthroline dyes which form colored complexes with iron).
- a dye e.g., ferene or ferrozine, or other pyridyl or phenanthroline dyes which form colored complexes with iron.
- the following method can be used to measure TIBC.
- the Fe solution can be added to the sample (e.g., serum) first, and allowed time to saturate all the available sites.
- the gel particles can be added to remove excess iron, the sample can be incubated, a magnetic field can be applied, and the measurement of supernatant iron can be made.
- the gel particles and high Fe concentration are mixed, and used as a single reagent.
- the sample e.g., serum
- the sample is added to this reagent and allowed time for the iron to transfer to the available sites on the serum proteins, a magnetic field is applied, and a measurement of supernatant iron is made.
- the affinity of iron for the sites in the sample e.g., serum protein sites
- the affinity of iron for the sites in the sample is much greater than the affinity of iron for the solid-phase ligand.
- the gel particles and the sample are first combined, then the high Fe concentration solution is added (wherein the serum proteins and the gel particles compete for the excess iron sample), allowed to incubate, a magnetic field applied, and a measurement of supernatant iron made.
- the alumina was neutral grade alumina purchased from Scientific Adsorbents, Inc. (Atlanta, GN)
- the agarose was type IN purchased from Sigma Chemical Co. (St. Louis, MO)
- the paramagnetic particles were MagAcrolein ® polyacrolein paramagnetic particles purchased from Cortex Biochem, Inc. (San Diego, CA). Correlation Study
- the magnetic separation TIBC methodology used an iron saturating reagent that contained 24 mg/L ferric chloride (i.e., 800ug/dL Fe ⁇ ) in 1 milliMolar (mM) citric acid solution (Reference Diagnostics product number 4021) and a magnetic separation reagent that contained 300 mg/mL magnetically responsive alumina particles in 20mm MOPS buffer, pH 7.5 (Reference Diagnostics product number 4022).
- the alumina column separation TIBC methodology used an iron saturating solution that contained not less than 500 ug/dL in 1mm citric acid solution and disposable columns that were prefilled with 300mg of alumina (J&S Medical Associates, Inc., product numbers ICM 117 and ICM 127).
- Serum iron reagents and a calibrator were used with ferene chromogen at 40 niM (DMA, Inc. product number 1580-300).
- the magnetic TIBC method is not interfered by: bilirubin at a level of up to at least 35 mg/dL; ascorbic acid at a level of up to at least 50 mg/dL; hemoglobin at a level of up to at least 200 mg/dL; and triglyceride at a level of up to at least 1450 mg/dL.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Inorganic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
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Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2000594639A JP2002535634A (en) | 1999-01-20 | 2000-01-19 | Magnetically responsive gel particles |
AU27317/00A AU2731700A (en) | 1999-01-20 | 2000-01-19 | Magnetically responsive gel particle |
EP00905670A EP1152888A4 (en) | 1999-01-20 | 2000-01-19 | Magnetically responsive gel particle |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11663399P | 1999-01-20 | 1999-01-20 | |
US60/116,633 | 1999-01-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000043199A1 true WO2000043199A1 (en) | 2000-07-27 |
Family
ID=22368343
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2000/001311 WO2000043199A1 (en) | 1999-01-20 | 2000-01-19 | Magnetically responsive gel particle |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1152888A4 (en) |
JP (1) | JP2002535634A (en) |
AU (1) | AU2731700A (en) |
WO (1) | WO2000043199A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2579032C (en) * | 2004-09-02 | 2011-11-29 | Cosmo Oil Co., Ltd. | Use of an .delta.-amino acid as thymocyte growth agent |
JP2006199810A (en) * | 2005-01-20 | 2006-08-03 | Yokohama National Univ | Composite particle and method for producing the same |
CN110102082B (en) * | 2019-05-21 | 2022-05-17 | 浙江省海洋水产研究所 | Method for removing heavy metal cadmium in shellfish enzymolysis liquid |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3981776A (en) * | 1967-02-16 | 1976-09-21 | Rolf Saxholm | Magnetically responsive, biologically active substance and associated methods and apparatus |
US4628037A (en) * | 1983-05-12 | 1986-12-09 | Advanced Magnetics, Inc. | Binding assays employing magnetic particles |
US4695392A (en) * | 1983-05-12 | 1987-09-22 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
US4992377A (en) * | 1967-02-16 | 1991-02-12 | Rolf Saxholm | Article for carrying out biological or chemical procedures containing magnetically responsive material |
US5091206A (en) * | 1987-10-26 | 1992-02-25 | Baxter Diagnostics Inc. | Process for producing magnetically responsive polymer particles and application thereof |
US5445970A (en) * | 1992-03-20 | 1995-08-29 | Abbott Laboratories | Magnetically assisted binding assays using magnetically labeled binding members |
US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2005019B (en) * | 1977-09-28 | 1982-08-18 | Technicon Instr | Magnetically attractable material |
DE69815282T2 (en) * | 1997-12-25 | 2004-05-06 | Tosoh Corp., Shinnanyo | Magnetic carrier, its manufacture and method for extracting nucleic acids |
-
2000
- 2000-01-19 WO PCT/US2000/001311 patent/WO2000043199A1/en not_active Application Discontinuation
- 2000-01-19 EP EP00905670A patent/EP1152888A4/en not_active Withdrawn
- 2000-01-19 JP JP2000594639A patent/JP2002535634A/en not_active Withdrawn
- 2000-01-19 AU AU27317/00A patent/AU2731700A/en not_active Abandoned
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3981776A (en) * | 1967-02-16 | 1976-09-21 | Rolf Saxholm | Magnetically responsive, biologically active substance and associated methods and apparatus |
US4992377A (en) * | 1967-02-16 | 1991-02-12 | Rolf Saxholm | Article for carrying out biological or chemical procedures containing magnetically responsive material |
US4992377B1 (en) * | 1967-02-16 | 1996-10-15 | Saxholm As | Article for carrying out biological or chemical procedures containing magnetically responsive material |
US4628037A (en) * | 1983-05-12 | 1986-12-09 | Advanced Magnetics, Inc. | Binding assays employing magnetic particles |
US4695392A (en) * | 1983-05-12 | 1987-09-22 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
US5091206A (en) * | 1987-10-26 | 1992-02-25 | Baxter Diagnostics Inc. | Process for producing magnetically responsive polymer particles and application thereof |
US5445970A (en) * | 1992-03-20 | 1995-08-29 | Abbott Laboratories | Magnetically assisted binding assays using magnetically labeled binding members |
US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
Non-Patent Citations (1)
Title |
---|
See also references of EP1152888A4 * |
Also Published As
Publication number | Publication date |
---|---|
JP2002535634A (en) | 2002-10-22 |
EP1152888A4 (en) | 2003-08-06 |
AU2731700A (en) | 2000-08-07 |
EP1152888A1 (en) | 2001-11-14 |
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