WO2000033635A3 - Primer-independent rna synthesis catalyzed by hcv polymerase - Google Patents

Primer-independent rna synthesis catalyzed by hcv polymerase Download PDF

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Publication number
WO2000033635A3
WO2000033635A3 PCT/US1999/029649 US9929649W WO0033635A3 WO 2000033635 A3 WO2000033635 A3 WO 2000033635A3 US 9929649 W US9929649 W US 9929649W WO 0033635 A3 WO0033635 A3 WO 0033635A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna
templates
methods
primer
nucleotides
Prior art date
Application number
PCT/US1999/029649
Other languages
French (fr)
Other versions
WO2000033635A2 (en
Inventor
Xin-Lai Sun
Q May Wang
Original Assignee
Lilly Co Eli
Sun Xin Lai
Q May Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli, Sun Xin Lai, Q May Wang filed Critical Lilly Co Eli
Priority to AU21818/00A priority Critical patent/AU2181800A/en
Publication of WO2000033635A2 publication Critical patent/WO2000033635A2/en
Publication of WO2000033635A3 publication Critical patent/WO2000033635A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Communicable Diseases (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Provided are methods for identifying inhibitors of primer-independent hepatitis C virus RNA-dependent RNA polymerase de novo RNA synthesis activity, methods for inhibiting the polymerase using such inhibitors, and methods for inhibiting hepatitis C virus replication. These methods employ homopolyC templates; homopolyU templates; synthetic heteropolymeric RNA templates comprising a cluster of cytidylate nucleotides, a cluster of uridylate nucleotides, or a mixed cluster of cytidylate and uridylate nucleotides; and synthetic or naturally occurring RNA templates comprising an RNA synthesis initiation pyrimidine nucleotide. Reactions can be carried out in buffers containing Mn++ alone, or in combination with Mg++ ions.
PCT/US1999/029649 1998-12-10 1999-12-10 Primer-independent rna synthesis catalyzed by hcv polymerase WO2000033635A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU21818/00A AU2181800A (en) 1998-12-10 1999-12-10 Primer-independent rna synthesis catalyzed by hcv polymerase

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11177398P 1998-12-10 1998-12-10
US60/111,773 1998-12-10
US11182598P 1998-12-11 1998-12-11
US60/111,825 1998-12-11

Publications (2)

Publication Number Publication Date
WO2000033635A2 WO2000033635A2 (en) 2000-06-15
WO2000033635A3 true WO2000033635A3 (en) 2001-03-15

Family

ID=26809212

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/029649 WO2000033635A2 (en) 1998-12-10 1999-12-10 Primer-independent rna synthesis catalyzed by hcv polymerase

Country Status (2)

Country Link
AU (1) AU2181800A (en)
WO (1) WO2000033635A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1278837A2 (en) * 2000-04-21 2003-01-29 Boehringer Ingelheim (Canada) Ltd. Internal de novo initiation sites of the hcv ns5b polymerase and use thereof
GB0029539D0 (en) * 2000-12-04 2001-01-17 Isis Innovation Method for identifying modulatorss of transcription
DE602005020761D1 (en) 2004-03-24 2010-06-02 Achillion Pharmaceuticals Inc R RNA IN A CELL-FREE SYSTEM AND IDENTIFICATION OF INHIBITORS OF RNA SYNTHESIS

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996037619A1 (en) * 1995-05-25 1996-11-28 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Method for reproducing in vitro the rna-dependent rna polymerase and terminal nucleotidyl transferase activities encoded by hepatitis c virus (hcv)
WO1997012033A1 (en) * 1995-09-27 1997-04-03 Emory University Recombinant hepatitis c virus rna replicase
WO2000040759A2 (en) * 1999-01-05 2000-07-13 Advanced Research And Technology Institute, Inc. Uses of flavivirus rna-dependent rna polymerases

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996037619A1 (en) * 1995-05-25 1996-11-28 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Method for reproducing in vitro the rna-dependent rna polymerase and terminal nucleotidyl transferase activities encoded by hepatitis c virus (hcv)
WO1997012033A1 (en) * 1995-09-27 1997-04-03 Emory University Recombinant hepatitis c virus rna replicase
WO2000040759A2 (en) * 1999-01-05 2000-07-13 Advanced Research And Technology Institute, Inc. Uses of flavivirus rna-dependent rna polymerases

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KAO C C ET AL: "De novo initiation of RNA synthesis by a recombinant flaviridae RNA-dependent RNA polymerase", VIROLOGY,ACADEMIC PRESS,ORLANDO,US, vol. 253, 1999, pages 1 - 7, XP002110174, ISSN: 0042-6822 *
LOHMAN V ET AL: "Biochemical and kinetic analyses of NS5B RNA-dependent RNA polymerase of the hepatitis C virus", VIROLOGY, vol. 249, 1998, pages 108 - 18, XP002141668 *
LOHMANN V ET AL: "BIOCHEMICAL PROPERTIES OF HEPATITIS C VIRUS NS5B RNA-DEPENDENT RNA POLYMERASE AND IDENTIFICATION OF AMINO ACID SEQUENCE MOTIFS ESSENTIAL FOR ENZYMATIC ACTIVITY", JOURNAL OF VIROLOGY,THE AMERICAN SOCIETY FOR MICROBIOLOGY,US, vol. 71, no. 11, November 1997 (1997-11-01), pages 8416 - 8428, XP000877461, ISSN: 0022-538X *
YUAN Z -H ET AL: "EXPRESSION, PURIFICATION, AND PARTIAL CHARACTERIZATION OF HCV RNA POLYMERASE", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,US,ACADEMIC PRESS INC. ORLANDO, FL, vol. 232, no. 1, 1997, pages 231 - 235, XP000877460, ISSN: 0006-291X *

Also Published As

Publication number Publication date
WO2000033635A2 (en) 2000-06-15
AU2181800A (en) 2000-06-26

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