WO2000022139A3 - Dna sequences for enzymatic synthesis of polyketide or heteropolyketide compounds - Google Patents

Dna sequences for enzymatic synthesis of polyketide or heteropolyketide compounds Download PDF

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Publication number
WO2000022139A3
WO2000022139A3 PCT/US1999/023535 US9923535W WO0022139A3 WO 2000022139 A3 WO2000022139 A3 WO 2000022139A3 US 9923535 W US9923535 W US 9923535W WO 0022139 A3 WO0022139 A3 WO 0022139A3
Authority
WO
WIPO (PCT)
Prior art keywords
component parts
polyketide
gene cluster
cosmid
pks
Prior art date
Application number
PCT/US1999/023535
Other languages
French (fr)
Other versions
WO2000022139A2 (en
WO2000022139A9 (en
Inventor
Stefan Beyer
Helmut Bloecker
Petra Brandt
Paul M Cino
Brian A Dougherty
Steven L Goldberg
Gerhard Hofle
Rolf-Joachim Mueller
Hans Reichenbach
Original Assignee
Biotechnolog Forschung Gmbh
Bristol Myers Squibb Co
Stefan Beyer
Helmut Bloecker
Petra Brandt
Paul M Cino
Brian A Dougherty
Steven L Goldberg
Gerhard Hofle
Mueller Rolf Joachim
Hans Reichenbach
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotechnolog Forschung Gmbh, Bristol Myers Squibb Co, Stefan Beyer, Helmut Bloecker, Petra Brandt, Paul M Cino, Brian A Dougherty, Steven L Goldberg, Gerhard Hofle, Mueller Rolf Joachim, Hans Reichenbach filed Critical Biotechnolog Forschung Gmbh
Priority to EP99953117A priority Critical patent/EP1119628A2/en
Priority to CA002346499A priority patent/CA2346499A1/en
Priority to JP2000576029A priority patent/JP2002527067A/en
Priority to AU65126/99A priority patent/AU6512699A/en
Publication of WO2000022139A2 publication Critical patent/WO2000022139A2/en
Publication of WO2000022139A9 publication Critical patent/WO2000022139A9/en
Publication of WO2000022139A3 publication Critical patent/WO2000022139A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters

Abstract

The invention consists of: (1) cloned Sorangium cellulosum polyketide synthase (PKS) biosynthetic cluster DNA; and (2) the nucleotide sequence and predicted protein coding sequences of the cloned DNA. The invention can be used for, but not limited to: (a) increasing yields of PKS product in Sorangium cellulosum (e.g., by amplification or genetic modification of the epothilone gene cluster or its component parts); (b) increasing yields of polyketide product in a heterologous system by transfer of the epothilone gene cluster or its component parts, which may be followed by amplification or genetic modification of the PKS gene cluster or its component parts; (c) modification of the polyketide product chemical structure in either Sorangium cellulosum or a heterologous host (e.g., by genetic modification of the epothilone gene cluster or its component parts; and (d) for the detection of genes and gene products involved in making polyketides or related molecules in other organisms (e.g., by hybridization or complementation assays). DNA sequence and analysis is presented for the following cosmids and plasmids: A2 cosmid; the pEPOcos6 region (overlapping of pEPOcos6 and pEPOcos7); pEPOcos8 cosmid; A5 cosmid; Sau4 (10 kb plasmid).
PCT/US1999/023535 1998-10-09 1999-10-11 Dna sequences for enzymatic synthesis of polyketide or heteropolyketide compounds WO2000022139A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP99953117A EP1119628A2 (en) 1998-10-09 1999-10-11 Dna sequences for enzymatic synthesis of polyketide or heteropolyketide compounds
CA002346499A CA2346499A1 (en) 1998-10-09 1999-10-11 Dna sequences for enzymatic synthesis of polyketide or heteropolyketide compounds
JP2000576029A JP2002527067A (en) 1998-10-09 1999-10-11 DNA sequences for enzymatic synthesis of polyketide or heteropolyketide compounds
AU65126/99A AU6512699A (en) 1998-10-09 1999-10-11 Dna sequences for enzymatic synthesis of polyketide or heteropolyketide compounds

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1998146493 DE19846493A1 (en) 1998-10-09 1998-10-09 DNA sequence coding for products involved in the biosynthesis of polyketide or heteropolyketide compounds, especially epothilone
DE19846493.2 1998-10-09

Publications (3)

Publication Number Publication Date
WO2000022139A2 WO2000022139A2 (en) 2000-04-20
WO2000022139A9 WO2000022139A9 (en) 2000-09-08
WO2000022139A3 true WO2000022139A3 (en) 2001-01-18

Family

ID=7883888

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/023535 WO2000022139A2 (en) 1998-10-09 1999-10-11 Dna sequences for enzymatic synthesis of polyketide or heteropolyketide compounds

Country Status (6)

Country Link
EP (1) EP1119628A2 (en)
JP (1) JP2002527067A (en)
AU (1) AU6512699A (en)
CA (1) CA2346499A1 (en)
DE (1) DE19846493A1 (en)
WO (1) WO2000022139A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU756699B2 (en) 1996-12-03 2003-01-23 Sloan-Kettering Institute For Cancer Research Synthesis of epothilones, intermediates thereto, analogues and uses thereof
US6121029A (en) * 1998-06-18 2000-09-19 Novartis Ag Genes for the biosynthesis of epothilones
IL143069A0 (en) 1998-11-20 2002-04-21 Kosan Biosciences Inc Recombinant methods and materials for producing epothilone and epothilone derivatives
US6410301B1 (en) 1998-11-20 2002-06-25 Kosan Biosciences, Inc. Myxococcus host cells for the production of epothilones
DE60114865T2 (en) * 2000-04-28 2006-07-27 Kosan Biosciences, Inc., Hayward HETEROLOGIST PREPARATION OF POLYCETIDES
US6998256B2 (en) 2000-04-28 2006-02-14 Kosan Biosciences, Inc. Methods of obtaining epothilone D using crystallization and /or by the culture of cells in the presence of methyl oleate
US7649006B2 (en) 2002-08-23 2010-01-19 Sloan-Kettering Institute For Cancer Research Synthesis of epothilones, intermediates thereto and analogues thereof
EP2186811A1 (en) 2002-08-23 2010-05-19 Sloan-Kettering Institute For Cancer Research Synthesis of epothilones, intermediates thereto, analogues and uses thereof
CN112941002B (en) * 2021-02-08 2023-04-25 中国科学院天津工业生物技术研究所 Recombinant strain of escherichia coli for producing dopamine as well as construction method and application thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993013663A1 (en) * 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides
US5716849A (en) * 1994-06-08 1998-02-10 Novartis Finance Corporation Genes for the biosynthesis of soraphen
WO1998022461A1 (en) * 1996-11-18 1998-05-28 GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) Epothilone c, d, e and f, production process, and their use as cytostatic as well as phytosanitary agents
WO1999066028A2 (en) * 1998-06-18 1999-12-23 Novartis Ag Genes for the biosynthesis of epothilones
WO2000031247A2 (en) * 1998-11-20 2000-06-02 Kosan Biosciences, Inc. Recombinant methods and materials for producing epothilone and epothilone derivatives

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993013663A1 (en) * 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides
US5716849A (en) * 1994-06-08 1998-02-10 Novartis Finance Corporation Genes for the biosynthesis of soraphen
WO1998022461A1 (en) * 1996-11-18 1998-05-28 GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) Epothilone c, d, e and f, production process, and their use as cytostatic as well as phytosanitary agents
WO1999066028A2 (en) * 1998-06-18 1999-12-23 Novartis Ag Genes for the biosynthesis of epothilones
WO2000031247A2 (en) * 1998-11-20 2000-06-02 Kosan Biosciences, Inc. Recombinant methods and materials for producing epothilone and epothilone derivatives

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BEYER S ET AL: "METABOLIC DIVERSITY IN MYXOBACTERIA: IDENTIFICATION OF THE MYXALAMID AND THE STIGMATELLIN BIOSYNTHETIC GENE CLUSTER OF STIGMATELLA AURANTIACA SG A15 AND A COMBINED POLYKETIDE(POLY)PEPTIDE GENE CLUSTER FROM THE EPOTHILONE PRODUCING STRAIN SORANGIUM CELLULOSUM SO CE90", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, vol. 1445, no. 2, 14 May 1999 (1999-05-14), AMSTERDAM, pages 185 - 195, XP000915670, ISSN: 0167-4781 *
SCHUPP T ET AL: "SORANGIUM CELLULOSUM (MYXOBACTERIUM) GENE CLUSTER FOR THE BIOSYNTHESIS OF THE MACROLIDE ANTIBIOTIC SORAPHEN A: CLONING, CHARACTERIZATION, AND HOMOLOGY TO POLYKETIDE SYNTHASE GENES FROM ACTINOMYCETES", JOURNAL OF BACTERIOLOGY, vol. 177, no. 13, July 1995 (1995-07-01), WASHINGTON, DC, pages 3673 - 3679, XP000893003, ISSN: 0021-9193 *

Also Published As

Publication number Publication date
JP2002527067A (en) 2002-08-27
DE19846493A1 (en) 2000-04-13
WO2000022139A2 (en) 2000-04-20
AU6512699A (en) 2000-05-01
CA2346499A1 (en) 2000-04-20
WO2000022139A9 (en) 2000-09-08
EP1119628A2 (en) 2001-08-01

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