WO2000022097A3 - Periferal blood mensenchymal precursor cells - Google Patents

Periferal blood mensenchymal precursor cells Download PDF

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Publication number
WO2000022097A3
WO2000022097A3 PCT/GB1999/003402 GB9903402W WO0022097A3 WO 2000022097 A3 WO2000022097 A3 WO 2000022097A3 GB 9903402 W GB9903402 W GB 9903402W WO 0022097 A3 WO0022097 A3 WO 0022097A3
Authority
WO
WIPO (PCT)
Prior art keywords
pbmpc
mensenchymal
precursor cells
periferal blood
blood
Prior art date
Application number
PCT/GB1999/003402
Other languages
French (fr)
Other versions
WO2000022097A2 (en
Inventor
Nathan Joseph Zvaifler
Lilia Marinova-Mutafchieva
Ravinder Nath Maini
Original Assignee
Kennedy Rheumatology Inst
Nathan Joseph Zvaifler
Marinova Mutafchieva Lilia
Ravinder Nath Maini
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kennedy Rheumatology Inst, Nathan Joseph Zvaifler, Marinova Mutafchieva Lilia, Ravinder Nath Maini filed Critical Kennedy Rheumatology Inst
Priority to EP99949225A priority Critical patent/EP1135464A2/en
Publication of WO2000022097A2 publication Critical patent/WO2000022097A2/en
Publication of WO2000022097A3 publication Critical patent/WO2000022097A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/21—Chemokines, e.g. MIP-1, MIP-2, RANTES, MCP, PF-4

Abstract

Isolated PBMPC's are described. Such PBMPC's behave like mesenchymal stem cells in that they lack differentiation markers and may be stimulated to differentiate into different types of cells. PBMPC's are obtainable by elutriating human blood. Such PBMPC's may be used for gene therapy or tissue engineering.
PCT/GB1999/003402 1998-10-14 1999-10-14 Periferal blood mensenchymal precursor cells WO2000022097A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP99949225A EP1135464A2 (en) 1998-10-14 1999-10-14 Periferal blood mensenchymal precursor cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9822434A GB9822434D0 (en) 1998-10-14 1998-10-14 Mesenchymal precursor cells
GB9822434.8 1998-10-14

Publications (2)

Publication Number Publication Date
WO2000022097A2 WO2000022097A2 (en) 2000-04-20
WO2000022097A3 true WO2000022097A3 (en) 2000-08-10

Family

ID=10840574

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/003402 WO2000022097A2 (en) 1998-10-14 1999-10-14 Periferal blood mensenchymal precursor cells

Country Status (3)

Country Link
EP (1) EP1135464A2 (en)
GB (1) GB9822434D0 (en)
WO (1) WO2000022097A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2326772B1 (en) * 2007-09-26 2010-07-26 Fundacion Progreso Y Salud PROCESS FOR OBTAINING MESENQUIMAL MOTHER CELLS WITH PLURIPOTENT CAPACITY.

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997039104A1 (en) * 1996-04-17 1997-10-23 Osiris Therapeutics, Inc. Cryopreservation and extensive subculturing of human mesenchymal stem cells
WO1999001145A1 (en) * 1997-07-03 1999-01-14 Osiris Therapeutics, Inc. Human mesenchymal stem cells from peripheral blood

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997039104A1 (en) * 1996-04-17 1997-10-23 Osiris Therapeutics, Inc. Cryopreservation and extensive subculturing of human mesenchymal stem cells
WO1999001145A1 (en) * 1997-07-03 1999-01-14 Osiris Therapeutics, Inc. Human mesenchymal stem cells from peripheral blood

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LAZARUS H M ET AL: "Human bone marrow-derived mesenchymal (stromal) progenitor cells (MPCs) cannot be recovered from peripheral blood progenitor cell collections.", JOURNAL OF HEMATOTHERAPY, (1997 OCT) 6 (5) 447-55., XP000906887 *
SANDMAIER B M ET AL: "Safety of infusing ex vivo expanded autologous mesenchymal stem cells with G-CSF-mobilized peripheral blood mononuclear cells into canines following lethal irradiation.", 40TH ANNUAL MEETING OF THE AMERICAN SOCIETY OF HEMATOLOGY;MIAMI BEACH, FLORIDA, USA; DECEMBER 4-8, 1998 BLOOD NOV. 15, 1998, vol. 92, no. 10 SUPPL. 1 PART 1-2, 15 November 1998 (1998-11-15), pages 289B, XP000906888, ISSN: 0006-4971 *

Also Published As

Publication number Publication date
EP1135464A2 (en) 2001-09-26
GB9822434D0 (en) 1998-12-09
WO2000022097A2 (en) 2000-04-20

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