WO2000017342A3 - Procedes et reactifs de recombinaison a mediation intronique - Google Patents

Procedes et reactifs de recombinaison a mediation intronique Download PDF

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Publication number
WO2000017342A3
WO2000017342A3 PCT/US1999/021929 US9921929W WO0017342A3 WO 2000017342 A3 WO2000017342 A3 WO 2000017342A3 US 9921929 W US9921929 W US 9921929W WO 0017342 A3 WO0017342 A3 WO 0017342A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequences
nucleic acid
reagents
intron
ligation
Prior art date
Application number
PCT/US1999/021929
Other languages
English (en)
Other versions
WO2000017342A2 (fr
Inventor
Kevin A Jarrell
Svetlana Mulcheeva
William Donahue
Original Assignee
Univ Boston
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Boston filed Critical Univ Boston
Priority to AU62583/99A priority Critical patent/AU6258399A/en
Publication of WO2000017342A2 publication Critical patent/WO2000017342A2/fr
Publication of WO2000017342A3 publication Critical patent/WO2000017342A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des procédés et des réactifs permettant une manipulation nouvelle des acides nucléiques. On utilise l'aptitude des séquences introniques (par exemple, dérivées d'introns des groupes I, II ou de type nucléaire pré-ARNm) à assurer la médiation d'opérations spécifiques de clivage et de ligature de molécules discontinues d'acides nucléiques. Ainsi, il est possible d'élaborer de nouveaux gènes et produits géniques par admixtion de constructions d'acides nucléiques comprenant des séquences d'acides nucléiques exoniques flanquées de séquences introniques capables d'orienter mutuellement l'une vers l'autre les séquences exoniques en trans-épissage. Par complémentation intermoléculaire, les séquences introniques flanquantes peuvent former un complexe réactif qui favorise les réactions de transestérification requises pour la ligature de séquences nucléotidiques discontinues, moyennant quoi il est possible d'élaborer un gène de recombinaison renfermant les exons ligaturés.
PCT/US1999/021929 1998-09-21 1999-09-21 Procedes et reactifs de recombinaison a mediation intronique WO2000017342A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU62583/99A AU6258399A (en) 1998-09-21 1999-09-21 Intron-mediated recombinant techniques and reagents

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10132898P 1998-09-21 1998-09-21
US60/101,328 1998-09-21

Publications (2)

Publication Number Publication Date
WO2000017342A2 WO2000017342A2 (fr) 2000-03-30
WO2000017342A3 true WO2000017342A3 (fr) 2000-11-09

Family

ID=22284075

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/021929 WO2000017342A2 (fr) 1998-09-21 1999-09-21 Procedes et reactifs de recombinaison a mediation intronique

Country Status (2)

Country Link
AU (1) AU6258399A (fr)
WO (1) WO2000017342A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365377B1 (en) * 1999-03-05 2002-04-02 Maxygen, Inc. Recombination of insertion modified nucleic acids
WO2002097080A2 (fr) * 2002-04-30 2002-12-05 Icon Genetics Ag Vecteurs d'amplification a base de transepissage

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997022250A1 (fr) * 1995-12-15 1997-06-26 Intronn Llc Molecules therapeutiques produites par trans-epissure
US5780272A (en) * 1993-09-10 1998-07-14 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
WO1998040519A1 (fr) * 1997-03-11 1998-09-17 Trustees Of Boston University Techniques et reactifs de recombinaison par introns

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780272A (en) * 1993-09-10 1998-07-14 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
WO1997022250A1 (fr) * 1995-12-15 1997-06-26 Intronn Llc Molecules therapeutiques produites par trans-epissure
WO1998040519A1 (fr) * 1997-03-11 1998-09-17 Trustees Of Boston University Techniques et reactifs de recombinaison par introns

Also Published As

Publication number Publication date
WO2000017342A2 (fr) 2000-03-30
AU6258399A (en) 2000-04-10

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