WO2000015222A1 - FUSED PYRIDINE INHIBITORS OF cGMP PHOSPHODIESTERASE - Google Patents

FUSED PYRIDINE INHIBITORS OF cGMP PHOSPHODIESTERASE Download PDF

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Publication number
WO2000015222A1
WO2000015222A1 PCT/US1999/021070 US9921070W WO0015222A1 WO 2000015222 A1 WO2000015222 A1 WO 2000015222A1 US 9921070 W US9921070 W US 9921070W WO 0015222 A1 WO0015222 A1 WO 0015222A1
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Prior art keywords
cycloalkyl
substituted
aryl
heterocyclo
heteroaryl
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PCT/US1999/021070
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French (fr)
Inventor
John E. Macor
Guixue Yu
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Bristol-Myers Squibb Company
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Priority to AU61438/99A priority Critical patent/AU751486B2/en
Priority to CA002342583A priority patent/CA2342583A1/en
Priority to EP99948211A priority patent/EP1113796A4/en
Priority to JP2000569806A priority patent/JP2002524512A/en
Publication of WO2000015222A1 publication Critical patent/WO2000015222A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/10—Drugs for genital or sexual disorders; Contraceptives for impotence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

  • the present invention relates ro fused pyridine compounds, to methods of using such compounds in the treatment of cGMP-associated conditions such as erectile dysfunction, and tc pharmaceutical compositions containing such compounds.
  • Erectile dysfunction is the inability to obtain and maintain a penile erection sufficient fcr sexual intercourse or other sexual expression.
  • a number of factors can place an individual at risk for this disorder, for example, trauma, pelvic surgery, hypercholesterolemia, ischemic heart disease, peripheral vascular disease, chronic renal failure, diabetes, cr the use cf medicaments such as certain antihypertensive medications or digoxin, or illicit drugs, cigarettes or alcohol.
  • Methods for the treatment cf erectile dysfunction include the use of vacuum devices and penile implants, as well as the administration of medicaments such as yohimbine, papaverine and apomorphine .
  • Improved methods for the treatment of this disorder are sought, however, as the aforementioned methods do not provide sufficient efficacy, and/or are accompanied by drawbacks or side effects such as erosion, pain, priapism or gastrointestinal discomfort.
  • cGMP cyclic guanosine 3' , 5' -monophosphate
  • cGMP PDE cGMP phosphodiesterase
  • cGMP PDE V a selective inhibitor of cGMP PDE Type V
  • the present invention provides novel compounds which are potent and selective inhibitors of cGMP PDE V which may be employed in the treatment of erectile dysfunction.
  • the present compounds can also be employed in the treatment of other disorders responding to the inhibition of cGMP PDE such as various cardiovascular disorders.
  • This invention is directed to the novel fused pyridine compounds of formulas I and II shown below including pharmaceutically acceptable salts thereof, pharmaceutical compositions containing one or more fused pyridines of formulas I and II, and the use of such compounds as inhibitors of cGMP PDE, especially type V. 0/15222
  • This invention is also directed to the use of the fused pyridine compounds of formula III shown below including pharmaceutically acceptable salts thereof as inhibitors of cGMP PDE, especially type V.
  • Ei is -O-Ri, -S-Ri, -NH-Ai-cycloalkyl, -NH-
  • E 2 is -NH-Ai-alkoxy, -NH-A ⁇ -C0 2 alkyl,
  • Ri is -Ai-cycloalkyl, -Ai-subsituted cycloalkyl, -Ai-
  • Xi is -0-A ⁇ -R 2 , -0-R 9 , -N(R 9 ) (R ⁇ o)
  • X 2 is -0-A ⁇ -R 25 , Rc a monocyclic ring
  • a spiro ring X 3 is -0-Rg , -O-A1-O-R 9 , -N ( R 9 ) ( R ⁇ o )
  • Ai is an alkylene or substituted alkylene bridge of 1 to 10 carbons.
  • Y is nitrogen or C(R 6 ).
  • Z is nitrogen or C(R ) with the proviso that at least one of Y or Z is nitrogen.
  • R 3 is hydrogen, alkyl, cycloalkyl, substituted cycloalkyl, substituted alkyl, -Ai-aryl, -A—substituted aryl, -Ai-cycloalkyl, or -A-substituted cycloalkyl.
  • R ⁇ and R7 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, -Ai-cycloalkyl, -Ai-substituted cycloalkyl, -Ai-aryl, Ai-substituted aryl, -Ai-heterocyclo, and Ai-heteroaryl.
  • R 4 is hydrogen, -N(R i2 ) (R i3 ) , -0R i2 or 1- or 3- imidazolyl .
  • a 2 is a direct bond, an alkylene or substituted alkylene bridge of 1 to 10 carbons, an alkenyl or substituted alkenyl bridge of 2 to 10 carbons having one or more double bonds, or an alkynyl or substituted alkynyl bridge of 2 to 10 carbons having one or more triple bonds.
  • R 2 is cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, heteroaryl, cycloalkyl-A 3 - cycloalkyl, cycloalkyl-A 3 -substituted cycloalkyl, cycloalkyl-A 3 -aryl, cycloalkyl-A 3 -substituted aryl, cycloalkyl-A 3 -heterocyclo, cycloalkyl-A 3 -heteroaryl, substituted cycloalkyl-A 3 -cycloalkyl, substituted cycloalkyl-A 3 -substituted cycloalkyl, substituted cycloalkyl-A 3 -aryl, substituted cycloalkyl-A 3 -substituted cycloalkyl, substituted cycloalkyl-A 3 -aryl, substituted cycloalky
  • aryl substituted cycloalkyl-A 3 -heterocyclo, substituted cycloalkyl-A 3 -heteroaryl, aryl-A 3 -cycloalkyl, aryl-A 3 - substituted cycloalkyl, aryl-A 3 -aryl, aryl-A 3 -substituted aryl, aryl-A 3 -heterocyclo, aryl-A 3 -heteroaryl, substituted aryl-A 3 -cycloalkyl, substituted aryl-A 3 -substituted cycloalkyl, substituted aryl-A 3 -aryl, substituted aryl-A 3 - substituted aryl, substituted aryl-A 3 -heterocyclo, substituted aryl-A 3 -heteroaryl, heterocyclo-A 3 -cycloalkyl, heterocyclo-A 3 -substituted
  • a : is not a direct bond, -NR n C0 2 R ⁇ 9 , - (C 0) N (Rn) CH 2 C0 2 R 19 , nitrogen when A 2 is alkynyl ending in a triple bond, or NH when A 2 is alkenyl ending in a double bond.
  • R 25 is cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, heteroaryl, cycloalkyl-A 3 - cycloalkyl, cycloalkyl-A 3 -substituted cycloalkyl, cycloalkyl-A 3 -aryl, cycloalkyl-A.
  • d is zero or an integer from 1 to 6.
  • e is zero or an integer from 1 to 6.
  • R 5 is hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, aryl, -Ai-aryl, substituted aryl, -Ai-substituted aryl, heterocyclo, -A ⁇ heterocyclo, heteroaryl or -Ai-heteroaryl.
  • g Rio, Rii/ R12, R ⁇ 3 , R15, i6 and R i9 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, heteroaryl, -Ai-cycloalkyl, -Ai-substituted cycloalkyl, -A x -aryl, -Ai-subsituted aryl, -Ai-heterocyclo and -Ai-heteroaryl, or R ⁇ 2 and R ⁇ 3 taken together with the N atom to which they are attached represent a heterocyclo ring. 0/15222
  • ⁇ o represents a monocyclic heterocyclo or heteroaryl ring of 4 to 8 atoms containing up to 3 additional heteroatoms (up to 2 additional heteroatoms when the ring is 4 atoms) which are selected from one or two oxygen atoms and/or one or two sulfur atoms and/or one, two or three nitrogen atoms.
  • R 2 ⁇ is attached to an available carbon or nitrogen atom and is hydrogen, alkyl, halogen, hydroxy, trifluoromethyl, amino, alkoxy or carboxy.
  • n is one or two.
  • m is zero or one.
  • R 23 is alkyl, -N(R 9 )(R ⁇ 0 ), -A x -hydroxy, -Ai-N (R 9 ) (R 10 ) , -Ai-carboxy, -A 2 -cycloalkyl, -A 2 -substituted cycloalkyl, -A 2 -aryl, -A 2 -substituted aryl, -A 2 -heterocyclo or -A 2 -heteroaryl .
  • -- ⁇ ' represents a fused bicyclic ring
  • monocyclic ring iits defined previously and represents a cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo or heteroaryl ring having a
  • alkyl refers to straight or branched chain hydrocarbon groups having 1 to 12 carbon atoms, preferably 1 to 8 carbon atoms. Lower alkyl groups, that is, alkyl groups of 1 to 4 carbon atoms, are most preferred.
  • alkoxy refers to an alkyl group bonded through an oxygen (-0-) .
  • alkylthio refers to an alkyl group bonded through a sulfur (-S-) .
  • substituted alkyl refers to an alkyl chain as defined above having one, two, or three substituents selected from halo, cyano, hydroxy, alkoxy, alkylthio, amino, -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2 , carboxy and -C0 2 -alkyl.
  • alkylene refers to a bridge of 1 to 10 carbons such as -CH 2 -, -(CH 2 ) 4 -, etc.
  • substituted alkylene refers to an alkylene bridge as previously defined having one, two, or 0/15222
  • alkyl substituted alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2 , carboxy, -C0 2 -alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, and heteroaryl.
  • substituted alkenyl refers to a bridge of 2 to 10 carbons containing at least one double bond as defined previously having, one, two or three substituents selected from alkyl, substituted alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino, -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2 , carboxy, -C0 2 -alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocycle, and heteroaryl.
  • alkynyl refers to a bridge of 2 to 10 carbons containing at least one triple bond such as -C ⁇ C-, -CH 2 -C ⁇ C-, etc.
  • substituted alkynyl refers to a bridge of 2 to 10 carbons containing at least one triple bond as defined previously having one, two, or three substituents selected from alkyl, subsituted alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino, -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2 , carboxy, -C0 2 -alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo and heteroaryl.
  • cycloalkyl refers to cyclic hydrocarbon groups of 3 to 9 carbon atoms, preferably 3 to 7 carbon atoms, which can be fully saturated or partially unsaturated. Also included within this definition are bicyclic cycloalkyl rings having a fused phenyl ring such 0/15222
  • bi C y C ii c rings having a carbon-carbon bridge of 3 or 4 carbons such as
  • aryl refers to phenyl, 1-naphthyl or 2-naphthyl, with phenyl being preferred.
  • oxygen atoms such as or
  • Substituted phenyl having one, two or three substituents as described above is the preferred substituted aryl.
  • heterocyclo refers to substituted and unsubstituted fully saturated or partially unsaturated 3 to 7 membered monocyclic groups, 7 to 11 membered bicyclic groups and 10 to 15 membered tricyclic groups which have at least one heteroatom (0, S or N) in at least one of the rings.
  • Each ring of the heterocyclo group containing a heteroatom can contain one or two oxygen or sulfur atoms and/or from one to four nitrogen atoms provided that the total number of heteroatoms in each ring is four or less, and further provided that each ring contains at least one carbon atom.
  • the fused ring completing the bicyclic and tricyclic groups may be a cycloalkyl, substituted cycloalkyl, aryl or subsituted aryl as defined above.
  • the bicyclic ring may also be formed by having a bridge of 2 or 3 carbons between available carbon atoms or between an available carbon and
  • the nitrogen and sulfur atoms may optionally be oxidized and the nitrogen atom may optionally be quaternized.
  • the heterocyclo group may be attached at any available nitrogen or carbon atom.
  • Exemplary monocyclic groups include azetidinyl, pyrrolidinyl, oxetanyl, imidazolinyl, oxazolidinyl, isoxazolinyl, thiazolidinyl, isothiazolidinyl, tetrahydrofuranyl, piperidinyl, piperazinyl, 2- oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolodinyl, 2- oxoazepinyl, azepinyl, 4-piperidonyl, tetrahydropyranyl, morpholinyl, thiamorpholinyl, thiamorpholinyl sulfoxide, thiamorpholinyl sulfone, 1, 3-dioxolane and tetrahydro- 1 , 1-dioxothienyi and the like.
  • Exemplary bicyclic heterocyclo groups include quinuclidiny
  • heteroaryl refers to substituted and unsubstituted aromatic 5 or 6 membered monocyclic groups, 9 or 10 membered bicyclic groups, and 11 to 14 membered tricyclic groups which have at least one heteroatom (0, S or N) in at least one of the rings.
  • Each ring of the heteroaryl group containing a heteroatom can contain one or two oxygen or sulfur atoms and/or from one to four nitrogen atoms provided that the total number of heteroatoms in each ring is four or less.
  • the fused rings completing the bicyclic and tricyclic groups may be a cycloalkyl, substituted cycloalkyl, aryl or substituted aryl as defined above.
  • the nitrogen and sulfur atoms may optionally be oxidized and the nitrogen atoms may optionally be quaternized.
  • Heteroaryl groups which are bicyclic or tricyclic must include at least one fully aromatic ring but the other fused ring or rings may be aromatic or non-aromatic.
  • the heteroaryl group may be attached at any available nitrogen or carbon atom of any ring.
  • the heteroaryl ring system may contain one, two or three substituents attached to an available carbon or nitrogen atom selected from alkyl, substituted alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino, 15222
  • Exemplary monocyclic heteroaryl groups include pyrrolyl, pyrazolyl, pyrazolinyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, thiadiazolyl, isothiazolyl, furanyl, thienyl, oxadiazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, and the like.
  • Exemplary bicyclic heteroaryl groups include indolyl, benzothiazolyl, benzoxazolyl, benzothienyl, quinolinyl, tetrahydroisoquinoiinyl, isoquinolinyl, benzimidazolyl, benzopyranyl, indolizinyl, benzofuranyl, chromonyl, coumarinyl, benzopyranyl, cinnolinyl, quinoxalinyl, indazolyl, pyrrolopyridyl, furopyridinyl, dihydroisoindolyl, tetrahydroquinolinyl and the like.
  • Exemplary tricyclic heteroaryl groups include carbazolyl, benzidolyl, phenanthrolinyl, acridinyl, phenanthridinyl, xanthenyl and the like.
  • Salts of the compounds of the formulas I, II and III may be formed, for example, by reacting a compound I, II or III with an amount of acid or base, such as an equivalent amount, in a medium such as one in which the salt precipitates or in an aqueous medium followed by lyophilization.
  • the compounds of formulas I, II and III which contain a basic moiety, such as , but not limited to an amine or a pyridine or imidazole ring, may form salts with a variety of organic and inorganic acids.
  • Exemplary acid addition salts include acetates (such as those formed with acetic acid or trihaloacetic acid, for example, trifluoroacetic acid) , adipates, alginates, ascorbates, aspartates, benzoates, benzenesulfonates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, cyclopentanepropionates, digluconates, dodecylsulfates, ethanesulfonates, fumarates, glucoheptanoates, glycerophosphates, hemisulfates, heptanoates, hexanoates, hydrochlorides (formed with hydroch
  • the compounds of formulas I, II and III which contain an acidic moiety, such as, but not limited to a carboxylic acid, may form salts with a variety of organic and inorganic bases.
  • Exemplary basic salts include ammonium salts, alkali metal salts such as sodium, lithium, and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, salts with organic bases (for example, organic amines) such as benzathines, dicyclohexylamines, hydrabamines (formed with N,N- bis (dehydroabietyl) ethylenediamine) , N-methyl-D- glucamines, N-methyl-D-glucamides, t-butyl amines, and salts with amino acids such as arginine, lysine and the like.
  • Basic nitrogen-containing groups may be quaternized with agents such as lower alkyl halides (e.g. methyl, ethyl, propyl, and butyl chlorides, bromides and iodides), dialkyl sulfates (e.g. dimethyl, diethyl, dibutyl, and diamyl sulfates), long chain halides (e.g. decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides), aralkyl halides (e.g. benzyl and phenethyl bromides), and others.
  • lower alkyl halides e.g. methyl, ethyl, propyl, and butyl chlorides, bromides and iodides
  • dialkyl sulfates e.g. dimethyl, diethyl, dibutyl, and diamyl sulfates
  • Prodrugs and solvates of the compounds of the invention are also contemplated herein.
  • the term "prodrug”, as employed herein, denotes a compound which, upon administration to a subject, undergoes chemical conversion by metabolic or chemical processes to yield a compound of formulas I, II or III or a salt and/or solvate thereof.
  • Solvates of the compounds of formulas I, II and III are preferably hydrates.
  • stereoisomers of the compounds of the invention may, for example, be substantially free of other isomers, or may be admixed, for example, as racemates or with all other, or other selected, stereoisomers.
  • the chiral centers of the present invention can have the S or R configuration as defined by the IUPAC 1974 Recommendations.
  • the compounds of the present invention may be prepared by methods such as those illustrated in the following Scheme I to IV. Solvents, temperatures, pressures, and other reaction conditions may readily be selected by one of ordinary skill in the art. Starting materials are commercially available or readily prepared by one of ordinary skill in the art.
  • High Speed Analoging may be employed in the preparation of compounds, for example, where the intermediates possess a carboxylic acid group or activated aromatic position, such as the 4 position of a 4-halopyridine .
  • substitutions on the fused five membered ring, such as pyrazoles, imidazoles, and triazoles, may also be achieved through HSA.
  • Exemplary carboxylic acid activating agents include carbonyldiimidazole, dicyclohexylcarbodiimide, pentofluorophenol trifluoroacetate, or 1- (3-dimethylaminopropyl) -3- ethylcarbodiimide.
  • Exemplary inert solvents include ethers, including tetrahydrofuran and dioxane, N,N- dimethylformamide, acetonitrile, or methylene chloride.
  • Compounds of formula IV can be prepared by the hydrolysis of compounds of formula V using a hydroxide source.
  • exemplary hydroxide sources include sodium 15222
  • hydroxide or lithium hydroxide examples include water, alcohols, and mixtures of ethers/water.
  • Compounds of formula V can be prepared by reacting compounds of formula VI with an amine, thiol or alcohol of the formulas Ei-H or E 2 -H.
  • the reaction may be performed in an inert solvent as appropriate, such as ethanol or N,N-dimethylformamide, in the presence of an appropriate base, such as triethylamine for amines and sodium hydride for thiol or alcohols, and is typically performed at elevated temperatures.
  • Compounds of formula VI can be prepared from compounds of either formula VIII or formula VII by reacting with an appropriate dehydrating agent typically under elevated temperatures.
  • exemplary dehydrating agents include P0C1 3 , PC1 5 , S0C1 2 and oxalyl chloride.
  • Compounds of formula VII can be prepared from compounds of formula VIII via an intramolecular cyclization typically perfomed at elevated temperatures in an inert solvent as appropriate or in neat form.
  • Compounds of formula VIII can be prepared by combining compounds of formula X and IX either neat or in an inert solvent as appropriate, typically such reaction is performed at elevated temperatures .
  • Compounds of formula lb, lib or IHb [wherein E is Ei or E 2 , X is X ⁇ , X 2 or X 3 , and Y and Z are both nitrogen] can be prepared via the aminolysis or esterification of a compound of formula XI using an appropriate carboxylic acid activating reagent and an appropriate amine or alcohol of the formula X-H in an inert solvent.
  • carboxylic acid activating agents include carbonyldiimidazole, dicyclohexylcarbodiinri.de, pentofluorophenol trifluoroacetate, or 1- (3- dimethylaminopropyl) -3-ethylcarbodiimide.
  • Exemplary inert solvents include ethers, including tetrahydrofuran and dioxane, N,N-dimethylformamide, acetonitrile, or methylene chloride.
  • Compounds of formula XI can be prepared from by the hydrolysis of compounds of formula XII using a hydroxide source.
  • exemplary hydroxide sources include sodium hydroxide or lithium hydroxide.
  • exemplary solvents include water, alcohols, and mixtures of ethers/water.
  • Compounds of formula XII can be prepared by treating a compound of formula XIII with a diazatizing reagent in an acidic aqueous medium.
  • Sodium nitrite is an exemplary 15222
  • diazatizing reagent and dilute (IN) HC1 is an exemplary reaction solvent.
  • Compounds of formula XIII can be prepared via the reduction of a compound of formula XIV in an inert solvent.
  • This reduction may, for example, be mediated via a platinum or palladium-catalyzed hydrogenation using platinum or palladium on carbon, hydrogen and an inert solvent such as ethanol or methanol or, alternatively, by use of a stoichiometric reducing agent, such as stannous(II) chloride, in an inert solvent such as ethyl acetate.
  • Compounds of formula XIV can be prepared by reacting compounds of formula XV with amines of the formula R 3 NH 2 .
  • the reaction may be performed in an inert solvent as appropriate, such as ethanol, in the presence of appropriate base, such as triethylamine, and typically under elevated temperatures.
  • Compounds of formula XV can be prepared by reacting compounds of formula XVI with an amine, thiol or alcohol of the formula E-H. The reaction may be performed in an inert solvent as appropriate, such as N,N- dimethylformamide, in the presence of an appropriate base, such as triethylamine for amines and sodium hydride for thiols or alcohols.
  • Methods of synthesis of compounds of formulas XVII, XVI, XV, XIV, XIII, XII, and XI are known to one skilled in the art. For example such methodology can be found in US 4,070,362, US 4,003,908, and US 4,048,182.
  • Compounds of formula XVII are either commercially available or prepared by methods known to one skilled in the art. Scheme III
  • Compounds cf formula Ic, lie and IIIc [wherein E is Ei or E 2 and X is Xi, X 2 or X 3 , Y is C(R 6 ) and Z is nitrogen] can be prepared via the aminolysis or esterification of a compound of formula XVIII using an appropriate carboxylic acid activating reagent and an appropriate amine or alcohol of the formula X-H in an inert solvent.
  • carboxylic acid activating agents include carbonyldiimidazole, dicyclohexylcarbodiimide, pentofluorophenol trifluoroacetate, or 1- (3-dimethylaminopropyl) -3- ethylcarbodiimide.
  • Exemplary inert solvents include ethers, including tetrahydrofuran and dioxane, N,N- dimethylformamide, acetonitrile, or methylene chloride.
  • Compounds of formula XVIII can be prepared from by the hydrolysis of compounds of formula XIX using a hydroxide source.
  • Exemplary hydroxide sources include sodium hydroxide or lithium hydroxide.
  • Exemplary solvents include water, alcohols, and mixtures of ethers/water.
  • activated esters include acid chlorides derived from R 6 - C0 2 H, N,N-dialkylamide acetals (including, for example, N, N-dimethylformamide dimethyl acetal) and activated esters derived from the reaction of R ⁇ -C0 2 H with exemplary carboxylic acid activating agents such as carbonyldiimidazole, dicyclohexylcarbodiiirri.de, pentofluorophenol trifluoroacetate, or l-(3- dimethylaminopropyl) -3-ethylcarbodiimide .
  • carboxylic acid activating agents such as carbonyldiimidazole, dicyclohexylcarbodiiirri.de, pentofluorophenol trifluoroacetate, or l-(3- dimethylaminopropyl) -3-ethylcarbodiimide .
  • Exemplary bases include sodium hydride, potassium hydride, cesium carbonate, potassium carbonate, potassium hexamethyldisilazide, and potassium t-butoxide.
  • Exemplary inert solvents include ethers, N,N- dimethyIformamide, and acetonitrile.
  • reaction may be performed in an inert solvent as appropriate, such as N,N-dimethylformamide, in the presence of an appropriate base, such as triethylamine for amines and sodium hydride for alcohols, and typically under elevated temperatures.
  • an inert solvent such as N,N-dimethylformamide
  • an appropriate base such as triethylamine for amines and sodium hydride for alcohols, and typically under elevated temperatures.
  • Compounds XX can be prepared by reacting compounds of formula XXI with an appropriate amine or alcohol of the formula X-H in an inert solvent in the presence of an appropriate base such as triethylamine.
  • inert solvents include ethers, including tetrahydrofuran and dioxane, N,N-dimethylformamide, acetonitrile, or methylene chloride.
  • Compounds XXI can be prepared from compounds of formula XXII by reacting with an appropriate dehydrating agent typically under elevated temperatures.
  • exemplary dehydrating agents include P0C1 3 , PCI 5 , S0C1 2 and oxalyl chloride.
  • Compounds XXII can be prepared from by the hydrolysis of compounds of formula VII using a hydroxide source.
  • exemplary hydroxide sources include sodium hydroxide or lithium hydroxide.
  • exemplary solvents include water, alcohols, and mixtures of ethers/water.
  • Y is nitrogen.
  • Z is nitrogen or C(R ).
  • Ei is -O-Ri, -NH-Ai-cycloalkyl, -NH-Ai-heterocyclo, or -NH-Ai-heteroaryl.
  • E 2 is -NH-Ai-alkoxy, , -NH-A substituted phenyl, or -NH-A ⁇ -C0 2 -alkyl .
  • Ri is -Ai-substituted phenyl.
  • Xi is -O-Ai-heteroaryl, -O-Ai-heterocyclo,
  • X 2 is -0-A 2 -heteroaryl
  • R 7 is hydrogen
  • R 4 is hydrogen
  • R 3 is straight or branched chain alkyl of 1 to 4 carbons .
  • Rs is hydrogen, alkyl, -C0 2 -alkyl, -Ai-phenyl, or -Ai-heteroaryl wherein alkyl is straight or branched chain of 1 to 4 carbons.
  • Ai is an alkylene or substituted alkylene bridge of
  • heterocyclo in the preferred definitions refers to a substituted or unsubstituted fully saturated or partially saturated 5 to 7 membered monocyclic rings containing one or two heteroatoms selected from oxygen, sulfur and nitrogen and bicyclic rings wherein the monocyclic ring as defined above is fused to a phenyl cr substituted phenyl or wherein a bridge of 2 or 3 carbons is present between available carbon and nitrogen atom.
  • the nitrogen and sulfur atoms may optionally be oxidized and the nitrogen atom may optionally be quaternized.
  • the heterocyclo group may be attached at any available nitrogen or carbon atom.
  • the heterocyclo ring may contain one or two substituents attached to an available carbon or nitrogen atom selected from alkyl, keto and -C0 2 -alkyl, wherein alkyl is straight or branched chain of 1 to 4 carbons.
  • heteroaryl in the preferred definitions refers to a substituted or unsubstituted aromatic 5 or 6 membered monocyclic ring containing one or two oxygen or sulfur atoms and/or from one to four nitrogen atoms provided that the total number of heteroatoms in the ring is four or less, and bicyclic rings wherein the monocyclic ring as defined above is fused to a phenyl or substituted phenyl.
  • the nitrogen and sulfur atoms may optionally be oxidized and the nitrogen atom may optionally be quaternized.
  • the heteroaryl group may be attached at any available nitrogen or carbon atom.
  • the heteroaryl ring may contain one or two substituents attached to an available carbon or nitrogen atom selected from straight or branched chain alkyl of 1 to 4 carbons and halo.
  • cycloalkyl in the preferred definitions refers to a fully saturated cyclic hydrocarbon group of 3 to 7 carbons and such cycloalkyl rings fused to a phenyl ring or such cycloalkyl rings 5 to 7 carbons having a carbon-carbon bridge of 3 or 4 carbons.
  • substituted phenyl refers to a phenyl ring having one, two, or three substituents selected from alkyl, halo, hydroxy, trifluoromethyl, alkoxy of 1 to 4 carbons, -N (alkyl) 2 , and S0 2 NH 2 wherein alkyl is straight or branched chain of 1 to 4 carbons, and a phenyl ring substituted with a fused five membered ketal, i.e.
  • R 9 , Rio, Rn, R ⁇ 2 , R13, R ⁇ 5 , Rie and R ⁇ 9 are independently selected from the group consisting of . hydrogen, straight or branched chain alkyl of 1 to 4 carbons, phenyl, subsituted phenyl and -Ai-phenyl.
  • a 3 is a direct bond, an alkylene bridge of 1 to 6 0
  • .s a 5 to 7 membered heterocyclo ring which can contain an additional nitrogen atom or can contain an oxygen or sulfur atom.
  • R 2 ⁇ is attached to an available carbon or nitrogen atom and is hydrogen, straight or branched chain alkyl of 1 to 4 carbons, hydroxy or amino.
  • R 23 is alkyl, -N(R 9 ) (Rio), or -A 2 -heteroaryl wherein alkyl is straight or branched chain of 1 to 4 carbons.
  • -J3> represents a fused bicyclic ring
  • Y, Z, E 2 , R 4 and R 3 are as defined for the preferred compounds of formula II, and
  • Y is nitrogen.
  • Z is CH.
  • R 3 is ethyl.
  • R 4 is hydrogen
  • Ei is or -0-CH 2 - disubstituted phenyl .
  • E 2 is -NH-CH 2 -disubstituted phenyl, 0/15222
  • disubstituted phenyl refers to a phenyl ring having two substituents independently selected from halogen and methoxy or wherein said disubstituted phenyl
  • Xi is -O-Ai-heterocyclo, -O-Ai-heteroaryl, -NH-A 2 -R 2 ,
  • X 2 is -O-Ai-heterocyclo, -O-Ai-heteroaryl, -NH-A 2 -R 25
  • heterocyclo and “heteroaryl” are as defined in the preferred definitions.
  • Y, Z, E 2 , R 4 and R 3 are as defined for the preferred compounds of formula II, and 0/15222
  • X 3 is -NH-A-OR 9 wherein A 2 and Rg are as defined for the preferred compounds of formula II.
  • Y is nitrogen .
  • Z is CH .
  • R 3 is ethyl .
  • R 4 is hydrogen .
  • Xi and X 2 are independently selected from the group consisting of
  • the compounds of the present invention inhibit cGMP PDE, and in particular are potent and selective inhibitors of cGMP PDE V.
  • the present compounds are useful in the treatment of cGMP-associated conditions.
  • a v cGMP-associated condition denotes a disorder which can be treated by inhibiting cGMP PDE or elevating the level of cGMP in a subject, wherein treatment comprises prevention, partial alleviation or cure of the disorder.
  • Inhibition of cGMP PDE or elevation of the cGMP level may occur locally, for example, within certain tissues of the subject, or more extensively throughout a subject being treated for such a disorder. Treatment may be facilitated wherein elevation of the cGMP level potentiates additional beneficial therapeutic effects, such as where elevation of the cGMP level potentiates the effects of endothelium-derived. relaxing factor.
  • the compounds of the present invention are useful for the treatment of a variety of cardiovascular diseases including, but not limited to, hypertension, angina (stable, unstable, and variant) , (congestive) heart failure, restenosis, atherosclerosis, and dyslipidemia, as well as reduced blood vessel patency, thrombus, both venous and arterial, myocardial infarction, peripheral vascular disease, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, glaucoma, diseases characterized by disorders of gut motility, and forms of cancer responsive to the inhibition of cGMP PDE.
  • cardiovascular diseases including, but not limited to, hypertension, angina (stable, unstable, and variant) , (congestive) heart failure, restenosis, atherosclerosis, and dyslipidemia, as well as reduced blood vessel patency, thrombus, both venous and arterial, myocardial infarction, peripheral vascular disease, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis,
  • these compounds are useful in the treatment of sexual dysfunction in both men (erectile dysfunction, for example, due to diabetes mellitus, spinal cord injury, radical prostatectomy, psychogenic etiology or any other cause) and women by improving blood flow to the genitalia, especially, the corpus cavernosum.
  • the present invention thus provides methods for the treatment of cGMP-associated conditions, comprising the step of administering to a subject in need thereof at least one compound of formulas I, II or III in an amount effective therefor.
  • Other therapeutic agents such as those described below may be employed with the inventive compounds in the present methods.
  • such other therapeutic agent (s) may be administered prior to, simultaneously with or following the administration of the compound (s) of the present invention.
  • the present invention also provides pharmaceutical compositions comprising at least one of the compounds of formulas I, II or III capable of treating a cGMP- associated condition in an amount effective therefor, and a pharmaceutically acceptable vehicle or diluent.
  • the compositions of the present invention may contain other therapeutic agents as described below, and may be formulated, for example, by employing conventional solid or liquid vehicles or diluents, as well as pharmaceutical additives of a type appropriate to the mode of desired administration (for example, excipients, binders, preservatives, stabilizers, flavors, etc.) according to techniques such as those well known in the art of pharmaceutical formulation.
  • the compounds of formulas I, II and III may be administered by any suitable means, for example, orally, such as in the form of tablets, capsules, granules or powders; sublingually; bucally; parenterally, such as by subcutaneous, intravenous, intramuscular, or intrasternal injection or infusion techniques (e.g., as sterile injectable aqueous or non-aqueous solutions or suspensions) ; nasally such as by inhalation spray; topically, such as in the form of a cream or ointment; or rectally such as in the form of suppositories; in dosage unit formulations containing non-toxic, pharmaceutically acceptable vehicles or diluents.
  • suitable means for example, orally, such as in the form of tablets, capsules, granules or powders; sublingually; bucally; parenterally, such as by subcutaneous, intravenous, intramuscular, or intrasternal injection or infusion techniques (e.g., as sterile injectable a
  • the present compounds may, for example, be administered in a form suitable for immediate release or extended release. Immediate release or extended release may be achieved by the use of suitable pharmaceutical compositions comprising the present compounds, or, particularly in the case of extended release, by the use of devices such as subcutaneous implants or osmotic pumps.
  • the present compounds may also be administered liposomally.
  • compositions for oral administration include suspensions which may contain, for example, microcrystalline cellulose for imparting bulk, alginic acid or sodium alginate as a suspending agent, methylcellulose as a viscosity enhancer, and sweeteners or flavoring agents such as those known in the art; and immediate release tablets which may contain, for example, microcrystalline cellulose, dicalcium phosphate, starch, magnesium stearate and/or lactose and/or other excipients, binders, extenders, disintegrants, diluents and lubricants such as those known in the art.
  • the compounds of formulas I, II and III may also be delivered through the oral cavity by sublingual and/or buccal administration.
  • Molded tablets, compressed tablets or freeze-dried tablets are exemplary forms which may be used.
  • Exemplary compositions include those formulating the present compound (s) with fast dissolving diluents such as mannitol, lactose, sucrose and/or cyclodextrins. Also included in such formulations may be high molecular weight excipients such as celluloses (Avicel®) or polyethylene glycols (PEG) .
  • Such formulations may also include an excipient to aid mucosal adhesion such as hydroxy propyl cellulose (HPC) , hydroxy propyl methyl cellulose (HPMC) , sodium carboxy methyl cellulose (SCMC) , maleic anhydride copolymer (e.g., Gantrez®) , and agents to control release such as polyacrylic copolymer (e.g., Carbopol® 934).
  • HPC hydroxy propyl cellulose
  • HPMC hydroxy propyl methyl cellulose
  • SCMC sodium carboxy methyl cellulose
  • agents to control release such as polyacrylic copolymer (e.g., Carbopol® 934).
  • Lubricants, glidants, flavors, coloring agents and stabilizers may also be added for ease of fabrication and use.
  • compositions for nasal aerosol or inhalation administration include solutions in saline which may contain, for example, benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, and/or other solubilizing or dispersing agents such as those known in the art.
  • compositions for parenteral administration include injectable solutions or suspensions which may contain, for example, suitable non- toxic, parenterally acceptable diluents or solvents, such as mannitol, 1, 3-butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid.
  • suitable non- toxic, parenterally acceptable diluents or solvents such as mannitol, 1, 3-butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid.
  • compositions for rectal administration include suppositories which may contain, for example, a suitable non-irritating excipient, such as cocoa butter, synthetic glyceride esters or polyethylene glycols, which 222
  • compositions for topical administration include a topical carrier such as Plastibase (mineral oil gelled with polyethylene) .
  • a topical carrier such as Plastibase (mineral oil gelled with polyethylene) .
  • the effective amount of a compound of the present invention may be determined by one of ordinary skill in the art, and includes exemplary dosage amounts for an adult human of from about 0.05 to 100 mg/kg of body weight of active compound per day, which may be administered in a single dose or in the form of individual divided doses, such as from 1 to 4 times per day. It will be understood that the specific dose level and frequency cf dosage for any particular subject may be varied and will depend upon a variety of factors including the activity of the specific compound employed, the metabolic stability and length of action of that compound, the species, age, body weight, general health, sex and diet of the subject, the mode and time of administration, rate of excretion, drug combination, and severity of the particular condition.
  • Preferred subjects for treatment include animals, most preferably mammalian species such as humans, and domestic animals such as dogs, cats, horses and the like, subject to cGMP- associated conditions.
  • the compounds of the present invention may be employed alone or in combination with each other and/or other suitable therapeutic agents useful in the treatment of cGMP-associated conditions such as other cGMP PDE inhibitors, particularly other cGMP PDE V inhibitors, prostanoids, ⁇ -adrenergic agonists, endothelin antagonists, angiotensin II (especially, subtype ATi) antagonists, angiotensin converting enzyme (ACE) inhibitors, renin inhibitors, and serotonin (5-HT 2c ) agonists.
  • cGMP PDE inhibitors particularly other cGMP PDE V inhibitors
  • prostanoids ⁇ -adrenergic agonists
  • endothelin antagonists angiotensin II (especially, subtype ATi) antagonists
  • angiotensin II (especially, subtype ATi) antagonists angiotensin converting enzyme (ACE) inhibitors
  • renin inhibitors especially, subtype ATi
  • Exemplary such other therapeutic agents include the following: phentolamine, yohimbine, papaverine, apomorphine, sildenafil (see Drugs of the Future, 22, 138-143 (1997)), pyrazolopyrimidinones as described in U.S. Patent Nos. 5,272,147; 5,250,534; 5,426,107; and
  • PDE V inhibitors selected from imidazoquinazolines (see WO 98/08848), carbazoles (see WO 97/03675, WO 97/03985 and WO 95/19978), imidazopurinones (see WO 97/19947), benzimidazoles (see WO 97/24334), pyrazoloquinolines (see U.S. Patent No. 5,488,055), anthranilic acid derivatives (see WO 95/18097), fused heterocycles (see WO 98/07430) and thienopyrimidines (see DE 19632423) ; and 5-HT 2c agonists selected from indoles (see J. Med. Chem . , 40, 2762-2769 (1997), EP 655440 and EP 657426).
  • the following assay can be employed in ascertaining the degree of activity of a compound as a cGMP PDE inhibitor.
  • Compounds described in the following Examples have been tested in this assay, and have shown activity.
  • Sonicated human platelet homogenates are prepared by the method of Seiler, et al. (Seiler, S., Gillespie, E., Arnold, A.J., Brassard, C.L., Meanwell, N.A. and Fleming, J.S., " Imidazoquinoline derivatives: potent inhibitors of platelet cAMP phosphodiesterase which elevate cAMP levels and activate protein kinase in platelets," Thrombosis Research, 62: 31-42 (1991)).
  • PDE V is abundant in human platelets, and accounts for approximately 90% of the cGMP hydrolytic activity in the homogenates.
  • PDE V can be resolved from other PDE activities in the homogenates by anion exchange chromatography on a fast protein liquid chromatography system (FPLC) using a Mono- Q anion exchange column (Pharmacia) eluted with a linear gradient of 10 mM - 450 mM NaCl.
  • FPLC fast protein liquid chromatography system
  • Pharmacia Mono- Q anion exchange column
  • the phosphodiesterase activity is assayed using a commercially available phosphodiesterase [ 3 H]cGMP scintillation proximity (SPA) assay kit (Amersham) .
  • SPA phosphodiesterase
  • the manufacturer's protocol is followed explicitly except that the reactions are carried out at room temperature and 3 mM nonradioactive cGMP is included in the suspension of SPA beads to prevent the synthesis of any additional radioactive products.
  • P0C1 3 phosphorus oxychloride
  • MgS0 4 magnesium sulfate
  • Na 2 S0 4 sodium sulfate
  • the resulting aqueous mixture was extracted with diethyl ether (3x150 mL), and the organic extracts were discarded.
  • the aqueous layer was acidified with 1 N HCI, and the resulting white precipitate was collected by filtration and washed sequentially with 1 N HCI, H 2 O, EtOH and Et 2 O to afford the desired 4-aminopyrazolopyridine.
  • the resulting product contained ⁇ 5% free pentafluorophenol and was used directly in subsequent reactions.
  • a second crop may be obtained from the mother liquors. If a solid is not obtained by this method, then purification of the extraction residue via column chromatography using silica gel and elution with 5% CH 2 CI 2 -CH 3 OH afforded the appropriate title compound.
  • Method B The appropriate 4-amino pyrazolopyridine-5-carboxylic acid (2.8 mmol) was suspended in anhydrous CH 2 Cl 2 (20 mL), and oxalyl chloride (0.72 mL, 8.4 mmol) was added to this mixture followed by 2 drops of DMF. The resulting suspension was stirred at rt for 1 h after the suspension cleared. The resulting reaction solution was evaporated under reduced pressure, and the residue was redissolved in anhydrous CH 2 CI 2 (20 mL). To this solution was added sequentially Et 3 N (1.9 mL, 14.0 mmol) and then the appropriate amine or alcohol of the formula X-H (4.2 mmol). The solution was stirred at rt for 2 hr.
  • R is hydrogen
  • Y is nitrogen
  • Z is CH
  • Ei is

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Abstract

Compounds of the formulas (I), (II) and (III) are useful as inhibitors of cGMP PDE, especially type V.

Description

FUSED PYRIDINE INHIBITORS OF cGMP PHOSPHODIESTERASE
Field of the Invention
The present invention relates ro fused pyridine compounds, to methods of using such compounds in the treatment of cGMP-associated conditions such as erectile dysfunction, and tc pharmaceutical compositions containing such compounds.
Background of the Invention Erectile dysfunction is the inability to obtain and maintain a penile erection sufficient fcr sexual intercourse or other sexual expression. A number of factors can place an individual at risk for this disorder, for example, trauma, pelvic surgery, hypercholesterolemia, ischemic heart disease, peripheral vascular disease, chronic renal failure, diabetes, cr the use cf medicaments such as certain antihypertensive medications or digoxin, or illicit drugs, cigarettes or alcohol. Methods for the treatment cf erectile dysfunction include the use of vacuum devices and penile implants, as well as the administration of medicaments such as yohimbine, papaverine and apomorphine . Improved methods for the treatment of this disorder are sought, however, as the aforementioned methods do not provide sufficient efficacy, and/or are accompanied by drawbacks or side effects such as erosion, pain, priapism or gastrointestinal discomfort.
As penile erection is dependent upon the presence of adequate levels of cyclic guanosine 3' , 5' -monophosphate (cGMP) , especially in corpora cavernosa tissue, 0/15222
administration of an inhibitor of a cGMP phosphodiesterase (cGMP PDE) (and particularly, a selective inhibitor of cGMP PDE Type V (cGMP PDE V) ) provides a means for achieving and maintaining an erection, and therefore for treating erectile dysfunction. See Trigo-Rocha et al., "Nitric Oxide and cGMP: mediators of pelvic nerve-stimulated erection in dogs," Am . J. Physiol . , Vol. 264 (Feb. 1993); Bowman et al., "Cyclic GMP mediates neurogenic relaxation in the bovine retractor penis muscle," Br. J. Pharma c. , 81 , 665- 674 (1984); and Rajfer et al., "Nitric Oxide as a Mediator of Relaxation of the Corpus Cavernosum in Response to Nonadrenergic, Noncholinergic Neurotransmission," New England J. Med. , 326, 2 , 90-94 (Jan. 1992). Sildenafii, for example, has been described as a phosphodiesterase Type V inhibitor useful for the treatment of erectile dysfunction. See Drugs of the Future, 22, 138-143 (1997).
The present invention provides novel compounds which are potent and selective inhibitors of cGMP PDE V which may be employed in the treatment of erectile dysfunction. In view of their activity, the present compounds can also be employed in the treatment of other disorders responding to the inhibition of cGMP PDE such as various cardiovascular disorders.
Summary of the Invention
This invention is directed to the novel fused pyridine compounds of formulas I and II shown below including pharmaceutically acceptable salts thereof, pharmaceutical compositions containing one or more fused pyridines of formulas I and II, and the use of such compounds as inhibitors of cGMP PDE, especially type V. 0/15222
(U
(ID
Figure imgf000005_0001
This invention is also directed to the use of the fused pyridine compounds of formula III shown below including pharmaceutically acceptable salts thereof as inhibitors of cGMP PDE, especially type V.
(in:
Figure imgf000005_0002
In the above formulas : Ei is -O-Ri, -S-Ri, -NH-Ai-cycloalkyl, -NH-
Ai-substituted cycloalkyl, -NH-Ai-heterocyclo, or -NH-Ai-heteroaryl . /15222
E2 is -NH-Ai-alkoxy, -NH-Aχ-C02alkyl,
-NH-Ai-aryl, or -NH-Ai-substituted
Figure imgf000006_0001
aryl
Ri is -Ai-cycloalkyl, -Ai-subsituted cycloalkyl, -Ai-
alkoxy, , -Ai-aryl, -A:-substituted aryl,
Figure imgf000006_0002
-Ai-heterocyclc, or -Ai-heteroaryl ,
Xi is -0-Aι-R2, -0-R9, -N(R9) (Rιo)
Figure imgf000006_0003
monocylic ring / (R9->) , a fused bicyclic ring
<R2ι>„
Figure imgf000006_0004
~A2 R25
X2 is -0-Aι-R25, Rc a monocyclic ring
a fused bicyclic ring m
Figure imgf000006_0006
Figure imgf000006_0005
or a spiro ring
Figure imgf000006_0007
X3 is -0-Rg , -O-A1-O-R9 , -N ( R9 ) ( Rιo )
Figure imgf000007_0001
Figure imgf000007_0002
Ai is an alkylene or substituted alkylene bridge of 1 to 10 carbons.
Y is nitrogen or C(R6).
Z is nitrogen or C(R ) with the proviso that at least one of Y or Z is nitrogen.
R3 is hydrogen, alkyl, cycloalkyl, substituted cycloalkyl, substituted alkyl, -Ai-aryl, -A—substituted aryl, -Ai-cycloalkyl, or -A-substituted cycloalkyl.
Rβ and R7 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, -Ai-cycloalkyl, -Ai-substituted cycloalkyl, -Ai-aryl, Ai-substituted aryl, -Ai-heterocyclo, and Ai-heteroaryl.
R4 is hydrogen, -N(Ri2) (Ri3) , -0Ri2 or 1- or 3- imidazolyl .
A2 is a direct bond, an alkylene or substituted alkylene bridge of 1 to 10 carbons, an alkenyl or substituted alkenyl bridge of 2 to 10 carbons having one or more double bonds, or an alkynyl or substituted alkynyl bridge of 2 to 10 carbons having one or more triple bonds. R2 is cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, heteroaryl, cycloalkyl-A3- cycloalkyl, cycloalkyl-A3-substituted cycloalkyl, cycloalkyl-A3-aryl, cycloalkyl-A3-substituted aryl, cycloalkyl-A3-heterocyclo, cycloalkyl-A3-heteroaryl, substituted cycloalkyl-A3-cycloalkyl, substituted cycloalkyl-A3-substituted cycloalkyl, substituted cycloalkyl-A3-aryl, substituted cycloalkyl-A3-substituted /15222
aryl, substituted cycloalkyl-A3-heterocyclo, substituted cycloalkyl-A3-heteroaryl, aryl-A3-cycloalkyl, aryl-A3- substituted cycloalkyl, aryl-A3-aryl, aryl-A3-substituted aryl, aryl-A3-heterocyclo, aryl-A3-heteroaryl, substituted aryl-A3-cycloalkyl, substituted aryl-A3-substituted cycloalkyl, substituted aryl-A3-aryl, substituted aryl-A3- substituted aryl, substituted aryl-A3-heterocyclo, substituted aryl-A3-heteroaryl, heterocyclo-A3-cycloalkyl, heterocyclo-A3-substituted cycloalkyl, heterocyclo-A3- aryl, heterocyclo-A3-substituted aryl, heterocyclo-A3- heterocyclo, heterocyclo-A3-heteroaryl, heteroaryl-A3- cycloalkyl, heteroaryl-A3-substituted cycloalkyl, heteroaryl-A3-aryl, heteroaryl-A3-heterocyclo, heteroaryl- A3-heteroaryl, cyano, -OR9, -SR9, -(C=0)R9, -N(R9)(Rι0), -C02R9, -(C=0)N(Ri2) (R13), -S02N(Rι2) (Ri3), -NRn (C=0) R19,
-NRu(C=0)N(R12) (R13) , -0-(C=0)N(R1 ) (R13) provided that A: is not a direct bond, -NRnC02Rι9, - (C=0) N (Rn) CH2C02R19, nitrogen when A2 is alkynyl ending in a triple bond, or NH when A2 is alkenyl ending in a double bond. R25 is cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, heteroaryl, cycloalkyl-A3- cycloalkyl, cycloalkyl-A3-substituted cycloalkyl, cycloalkyl-A3-aryl, cycloalkyl-A.3-substituted aryl, cycloalkyl-A3-heterocyclo, cycloalkyl-A3-heteroaryl, subsituted cycloalkyl-A3-cycloalkyl, substituted cycloalkyl-A3-substituted cycloalkyl, substituted cycloalkyl-A3-aryl, substituted cycloalkyl-A3-substituted aryl, substituted cycloalkyl-A3-heterocyclo, substituted cycloalkyl-A3-heteroaryl, aryl-A3-cycloalkyl, aryl-A3- substituted cycloalkyl, aryl-A3-aryl, aryl-A3-substituted aryl, aryl-A3-heterocyclo, aryl-A3-heteroaryl, substituted aryl-A3-cycloalkyl, substituted aryl-A3-substituted cycloalkyl, substituted aryl-A3-aryl, substituted aryl-A3-substituted aryl, substituted aryl-A3- heterocyclo, substituted aryl-A3-heteroaryl, heterocyclo- 15222
A3-cycloalkyl, heterocyclo-A3-substituted cycloalkyl, heterocyclo-A3-aryl, heterocyclo-A3-substituted aryl, heterocyclo-A3-heterocyclo, heterocyclo-A3-heteroaryl, heteroaryl-A3-cycloalkyl, heteroaryl-A3-substituted cycloalkyl, heteroaryl-A3-aryl, heteroaryl-A3-substituted aryl, heteroaryi-A3-heterocyclo, heteroaryl-A3-heteroaryl, cyano, -S-R9, -;C=0)RU/ -C02R19, - (C=0) N (R12) (R13) , -SO2N(R12)R13),-NF9(C=O)R10, -NRU (C=0) N (R12) (R13) , -0-(C=0)N(R12) (R:3) provided that A2 is not a direct bond, -NR C02Ri9, - (C=0)N(Rn)CH2C02R19, nitrogen when A2 is alkynyl ending in a triple bond, or NH when A2 is alkenyl ending in a double bond.
A3 is a direct bond, an alkylene or substituted alkylene bridge of 1 to 10 carbons, an alkenyl or substituted alkenyl bridge of 2 to 10 having one or more double bonds, an alkynyl or substituted alkynyl bridge of 2 to 10 carbons having one or more triple bonds, -(CH2)d-0-(CH2)e-, -(CH2)d-S-(CH2)e-, or -(CH2)d-(C=0)-(CH2)e-. d is zero or an integer from 1 to 6. e is zero or an integer from 1 to 6. R5 is hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, aryl, -Ai-aryl, substituted aryl, -Ai-substituted aryl, heterocyclo, -Aι~ heterocyclo, heteroaryl or -Ai-heteroaryl. g Rio, Rii/ R12, Rι3, R15, i6 and Ri9 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, heteroaryl, -Ai-cycloalkyl, -Ai-substituted cycloalkyl, -Ax-aryl, -Ai-subsituted aryl, -Ai-heterocyclo and -Ai-heteroaryl, or Rι2 and Rχ3 taken together with the N atom to which they are attached represent a heterocyclo ring. 0/15222
■o represents a monocyclic heterocyclo or heteroaryl ring of 4 to 8 atoms containing up to 3 additional heteroatoms (up to 2 additional heteroatoms when the ring is 4 atoms) which are selected from one or two oxygen atoms and/or one or two sulfur atoms and/or one, two or three nitrogen atoms.
R2ι is attached to an available carbon or nitrogen atom and is hydrogen, alkyl, halogen, hydroxy, trifluoromethyl, amino, alkoxy or carboxy. R22 is attached to an available carbon or nitrogen atom and is keto, -(C=0)R23, -C02-R25, -NH- (C=0) -R23, -N (alkyl) 2, -Ai-hydroxy, -Ai-N (R9) (R10; , -Ai-alkoxy, -Ai- carboxy, -A2-cycloalkyl, -A2-substituted cycloalkyl, -A2- aryl, -A2-substituted aryl, -A2-heterocyclo, or -A2-heteroaryl . n is one or two. m is zero or one.
R23 is alkyl, -N(R9)(Rι0), -Ax-hydroxy, -Ai-N (R9) (R10) , -Ai-carboxy, -A2-cycloalkyl, -A2-substituted cycloalkyl, -A2-aryl, -A2-substituted aryl, -A2-heterocyclo or -A2-heteroaryl .
--^' represents a fused bicyclic ring wherein
the monocyclic ring v*-^ is defined previously and
Figure imgf000010_0001
represents a cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo or heteroaryl having two carbon atoms in common with the monocyclic ring
"O /15222
"0Θ represents a spiro ring wherein the
monocyclic ring
Figure imgf000011_0001
iits defined previously and represents a cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo or heteroaryl ring having a
common carbon with the monocyclic ring •o
Detailed Description of the Invention
The following are definitions of terms used in this specification. The initial definition provided for a group or term herein applies to that group or term throughout the present specification, individually or as part of another group, unless otherwise indicated. The term "alkyl" refers to straight or branched chain hydrocarbon groups having 1 to 12 carbon atoms, preferably 1 to 8 carbon atoms. Lower alkyl groups, that is, alkyl groups of 1 to 4 carbon atoms, are most preferred. The term "alkoxy" refers to an alkyl group bonded through an oxygen (-0-) . The term "alkylthio" refers to an alkyl group bonded through a sulfur (-S-) .
The term "substituted alkyl" refers to an alkyl chain as defined above having one, two, or three substituents selected from halo, cyano, hydroxy, alkoxy, alkylthio, amino, -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2, carboxy and -C02-alkyl.
The term "alkylene" refers to a bridge of 1 to 10 carbons such as -CH2-, -(CH2)4-, etc. The term "substituted alkylene" refers to an alkylene bridge as previously defined having one, two, or 0/15222
three substituents selected from alkyl, substituted alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2, carboxy, -C02-alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, and heteroaryl.
The term "alkenyl" refers to a bridge of 2 to 10 carbons containing at least one double bond such as -CH=CH-, -CH2-CH=CH-, etc.
The term "subsituted alkenyl" refers to a bridge of 2 to 10 carbons containing at least one double bond as defined previously having, one, two or three substituents selected from alkyl, substituted alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino, -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2, carboxy, -C02-alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocycle, and heteroaryl. the term "alkynyl" refers to a bridge of 2 to 10 carbons containing at least one triple bond such as -C≡C-, -CH2-C≤C-, etc. The term "substituted alkynyl" refers to a bridge of 2 to 10 carbons containing at least one triple bond as defined previously having one, two, or three substituents selected from alkyl, subsituted alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino, -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2, carboxy, -C02-alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo and heteroaryl.
The term "cycloalkyl" refers to cyclic hydrocarbon groups of 3 to 9 carbon atoms, preferably 3 to 7 carbon atoms, which can be fully saturated or partially unsaturated. Also included within this definition are bicyclic cycloalkyl rings having a fused phenyl ring such 0/15222
as
Figure imgf000013_0001
biCyCiic rings having a carbon-carbon bridge of 3 or 4 carbons such as
Figure imgf000013_0002
The term "substituted cycloalkyl" refers to such cycloalkyl groups as defined above having one, two or three substituents selected from alkyl, substituted, alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino, -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2, carboxy, -C02-alkyl, ketc, =N-0H, =N-0-lower alkyl, and a five c six membered ketal, i.e. 1, 3-dioxolane or 1,3-dioxane.
The term "aryl" refers to phenyl, 1-naphthyl or 2-naphthyl, with phenyl being preferred.
The term "substituted aryl" refers to such aryl groups as defined previously having one, two or three substituents selected from alkyl, substituted alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino, -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2, carboxy, -C02-alkyl, keto, -(C=0)NH2, - (C=0) NH (alkyl) , - (C=0)NH (cycloalkyl) , - (C=0) N (alkyl) 2, - (C=0) alkyl,
-S02NH2, -0-(C=0) alkyl, -NH2-CH2-carboxy, -NH-CH2-C02- alkyl, and a five or six membered ring containing two
oxygen atoms such as
Figure imgf000013_0003
or
Substituted phenyl having one, two or
Figure imgf000013_0004
three substituents as described above is the preferred substituted aryl.
The term "halo" refers to chloro, bromo, fluoro and iodo. The term "heterocyclo" refers to substituted and unsubstituted fully saturated or partially unsaturated 3 to 7 membered monocyclic groups, 7 to 11 membered bicyclic groups and 10 to 15 membered tricyclic groups which have at least one heteroatom (0, S or N) in at least one of the rings. Each ring of the heterocyclo group containing a heteroatom can contain one or two oxygen or sulfur atoms and/or from one to four nitrogen atoms provided that the total number of heteroatoms in each ring is four or less, and further provided that each ring contains at least one carbon atom. The fused ring completing the bicyclic and tricyclic groups may be a cycloalkyl, substituted cycloalkyl, aryl or subsituted aryl as defined above. The bicyclic ring may also be formed by having a bridge of 2 or 3 carbons between available carbon atoms or between an available carbon and
nitrogen atoms such as
Figure imgf000014_0001
The nitrogen and sulfur atoms may optionally be oxidized and the nitrogen atom may optionally be quaternized. The heterocyclo group may be attached at any available nitrogen or carbon atom. The heterocyclo ring may contain one, two or three substituents attached to an available carbon or nitrogen atom selected from alkyl, substituted alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino, -NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2, carboxy, -C02-alkyl, keto, -(C=0)NH2,
- (C=0)NH (alkyl) , - (C=0) NH (cycloalkyl) , - (C=0) N (alkyl) 2, -(C=0) alkyl, -0- (C=0) alkyl, -NH-CH2-carboxy, -NH-CH2-C02-alkyl, =N-0H, =N-0-alkyl, and a five or six membered ring, i.e., 1, 3-dioxolane or 1,3-dioxane.
Exemplary monocyclic groups include azetidinyl, pyrrolidinyl, oxetanyl, imidazolinyl, oxazolidinyl, isoxazolinyl, thiazolidinyl, isothiazolidinyl, tetrahydrofuranyl, piperidinyl, piperazinyl, 2- oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolodinyl, 2- oxoazepinyl, azepinyl, 4-piperidonyl, tetrahydropyranyl, morpholinyl, thiamorpholinyl, thiamorpholinyl sulfoxide, thiamorpholinyl sulfone, 1, 3-dioxolane and tetrahydro- 1 , 1-dioxothienyi and the like. Exemplary bicyclic heterocyclo groups include quinuclidinyl .
The term "heteroaryl" refers to substituted and unsubstituted aromatic 5 or 6 membered monocyclic groups, 9 or 10 membered bicyclic groups, and 11 to 14 membered tricyclic groups which have at least one heteroatom (0, S or N) in at least one of the rings. Each ring of the heteroaryl group containing a heteroatom can contain one or two oxygen or sulfur atoms and/or from one to four nitrogen atoms provided that the total number of heteroatoms in each ring is four or less. The fused rings completing the bicyclic and tricyclic groups may be a cycloalkyl, substituted cycloalkyl, aryl or substituted aryl as defined above. The nitrogen and sulfur atoms may optionally be oxidized and the nitrogen atoms may optionally be quaternized. Heteroaryl groups which are bicyclic or tricyclic must include at least one fully aromatic ring but the other fused ring or rings may be aromatic or non-aromatic. The heteroaryl group may be attached at any available nitrogen or carbon atom of any ring. The heteroaryl ring system may contain one, two or three substituents attached to an available carbon or nitrogen atom selected from alkyl, substituted alkyl, halo, cyano, hydroxy, alkoxy, alkylthio, amino, 15222
-NH (alkyl), -NH (cycloalkyl) , -N (alkyl) 2, carboxy, -C02- alkyl, keto, -(C=0) NH2, - (C=0) NH (alkyl) , - (C=0)NH (cycloalkyl) , - (C=0) N (alkyl) 2, - (C=0) alkyl, -0-(C=0) alkyl, -NH-CH2-carboxy, -NH-CH2-CH2-C02-alkyl, and a five or six membered ring, i.e., 1, 3-dioxolane or 1, 3-dioxane.
Exemplary monocyclic heteroaryl groups include pyrrolyl, pyrazolyl, pyrazolinyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, thiadiazolyl, isothiazolyl, furanyl, thienyl, oxadiazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, and the like. Exemplary bicyclic heteroaryl groups include indolyl, benzothiazolyl, benzoxazolyl, benzothienyl, quinolinyl, tetrahydroisoquinoiinyl, isoquinolinyl, benzimidazolyl, benzopyranyl, indolizinyl, benzofuranyl, chromonyl, coumarinyl, benzopyranyl, cinnolinyl, quinoxalinyl, indazolyl, pyrrolopyridyl, furopyridinyl, dihydroisoindolyl, tetrahydroquinolinyl and the like. Exemplary tricyclic heteroaryl groups include carbazolyl, benzidolyl, phenanthrolinyl, acridinyl, phenanthridinyl, xanthenyl and the like.
Throughout the specification, groups and substituents thereof may be chosen to provide stable moieties and compounds . The compounds of formulas I, II and III form salts which are also within the scope of this invention. Reference to a compound of formulas I, II or III herein is understood to include reference to salts thereof, unless otherwise indicated. The term "salt(s)", as employed herein, denotes acidic and/or basic salts formed with inorganic and/or organic acids and bases. In addition, when a compound of formula I contains a both a basic moiety, such as, but not limited to a pyridine or imidazole, and an acidic moiety, such as, but not limited to a carboxylic acid, zwitterions ("inner salts") may be 22
formed and are included within the term "salt(s)" as used herein. Pharmaceutically acceptable (i.e., non-toxic, physiologically acceptable) salts are preferred, although other salts are also useful, e.g., in isolation or purification steps which may be employed during preparation. Salts of the compounds of the formulas I, II and III may be formed, for example, by reacting a compound I, II or III with an amount of acid or base, such as an equivalent amount, in a medium such as one in which the salt precipitates or in an aqueous medium followed by lyophilization.
The compounds of formulas I, II and III which contain a basic moiety, such as , but not limited to an amine or a pyridine or imidazole ring, may form salts with a variety of organic and inorganic acids. Exemplary acid addition salts include acetates (such as those formed with acetic acid or trihaloacetic acid, for example, trifluoroacetic acid) , adipates, alginates, ascorbates, aspartates, benzoates, benzenesulfonates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, cyclopentanepropionates, digluconates, dodecylsulfates, ethanesulfonates, fumarates, glucoheptanoates, glycerophosphates, hemisulfates, heptanoates, hexanoates, hydrochlorides (formed with hydrochloric acid), hydrobromides (formed with hydrogen bromide) , hydroiodides, 2-hydroxyethanesulfonates, lactates, maleates (formed with maleic acid) , methanesulfonates (formed with methanesulfonic acid.) , 2-naphthalenesulfonates, nicotinates, nitrates, oxalates, pectinates, persulfates, 3-phenylpropionates, phosphates, picrates, pivalates, propionates, salicylates, succinates, sulfates (such as those formed with sulfuric acid) , sulfonates (such as those mentioned herein) , tartrates, thiocyanates, toluenesulfonates such as tosylates, undecanoates, and the like. /15222
The compounds of formulas I, II and III which contain an acidic moiety, such as, but not limited to a carboxylic acid, may form salts with a variety of organic and inorganic bases. Exemplary basic salts include ammonium salts, alkali metal salts such as sodium, lithium, and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, salts with organic bases (for example, organic amines) such as benzathines, dicyclohexylamines, hydrabamines (formed with N,N- bis (dehydroabietyl) ethylenediamine) , N-methyl-D- glucamines, N-methyl-D-glucamides, t-butyl amines, and salts with amino acids such as arginine, lysine and the like. Basic nitrogen-containing groups may be quaternized with agents such as lower alkyl halides (e.g. methyl, ethyl, propyl, and butyl chlorides, bromides and iodides), dialkyl sulfates (e.g. dimethyl, diethyl, dibutyl, and diamyl sulfates), long chain halides (e.g. decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides), aralkyl halides (e.g. benzyl and phenethyl bromides), and others.
Prodrugs and solvates of the compounds of the invention are also contemplated herein. The term "prodrug", as employed herein, denotes a compound which, upon administration to a subject, undergoes chemical conversion by metabolic or chemical processes to yield a compound of formulas I, II or III or a salt and/or solvate thereof. Solvates of the compounds of formulas I, II and III are preferably hydrates.
Compounds of the formulas I, II and III and salts thereof, may exist in their tautomeric form (for example, as an amide or imino ether) . All such tautomeric forms are contemplated herein as part of the present invention. All stereoisomers of the present compounds, such as those which may exist due to asymmetric carbons on various substituents, including enantiomeric forms (which /15222
may exist even in the absence of asymmetric carbons) and diastereomeric forms, are contemplated within the scope of this invention. Individual stereoisomers of the compounds of the invention may, for example, be substantially free of other isomers, or may be admixed, for example, as racemates or with all other, or other selected, stereoisomers. The chiral centers of the present invention can have the S or R configuration as defined by the IUPAC 1974 Recommendations.
Methods of Preparation
The compounds of the present invention may be prepared by methods such as those illustrated in the following Scheme I to IV. Solvents, temperatures, pressures, and other reaction conditions may readily be selected by one of ordinary skill in the art. Starting materials are commercially available or readily prepared by one of ordinary skill in the art.
High Speed Analoging (HSA) may be employed in the preparation of compounds, for example, where the intermediates possess a carboxylic acid group or activated aromatic position, such as the 4 position of a 4-halopyridine . In the same manner, substitutions on the fused five membered ring, such as pyrazoles, imidazoles, and triazoles, may also be achieved through HSA.
222
SCHEME I
Figure imgf000020_0001
Compounds of formulas la, Ila and Ilia [wherein E is Ei or E2, X is Xi, X2 or X3, Y is nitrogen, and Z is C(R )j can be prepared via the aminolysis or esterification of a compound of formula IV using an appropriate carboxylic acid activating reagent and an appropriate amine or alcohol of the formulas Xi-H, X2-H or X3-H in an inert solvent. Exemplary carboxylic acid activating agents include carbonyldiimidazole, dicyclohexylcarbodiimide, pentofluorophenol trifluoroacetate, or 1- (3-dimethylaminopropyl) -3- ethylcarbodiimide. Exemplary inert solvents include ethers, including tetrahydrofuran and dioxane, N,N- dimethylformamide, acetonitrile, or methylene chloride.
Compounds of formula IV can be prepared by the hydrolysis of compounds of formula V using a hydroxide source. Exemplary hydroxide sources include sodium 15222
hydroxide or lithium hydroxide. Exemplary solvents include water, alcohols, and mixtures of ethers/water.
Compounds of formula V can be prepared by reacting compounds of formula VI with an amine, thiol or alcohol of the formulas Ei-H or E2-H. The reaction may be performed in an inert solvent as appropriate, such as ethanol or N,N-dimethylformamide, in the presence of an appropriate base, such as triethylamine for amines and sodium hydride for thiol or alcohols, and is typically performed at elevated temperatures.
Compounds of formula VI can be prepared from compounds of either formula VIII or formula VII by reacting with an appropriate dehydrating agent typically under elevated temperatures. Exemplary dehydrating agents include P0C13, PC15, S0C12 and oxalyl chloride. Compounds of formula VII can be prepared from compounds of formula VIII via an intramolecular cyclization typically perfomed at elevated temperatures in an inert solvent as appropriate or in neat form. Compounds of formula VIII can be prepared by combining compounds of formula X and IX either neat or in an inert solvent as appropriate, typically such reaction is performed at elevated temperatures .
Compounds of formula X and formula IX are either commercially available or available via methods known to one skilled in the art. For example, compounds of formula X may be prepared as described in French Patent 1,403,372 [ Chemical Abstracts, 1965, Volume 63, 14871a]. /15222
SCHEME I I
Figure imgf000022_0001
XVII XVI XV XIV
Figure imgf000022_0002
Compounds of formula lb, lib or IHb [wherein E is Ei or E2, X is Xλ , X2 or X3, and Y and Z are both nitrogen] can be prepared via the aminolysis or esterification of a compound of formula XI using an appropriate carboxylic acid activating reagent and an appropriate amine or alcohol of the formula X-H in an inert solvent. Exemplary carboxylic acid activating agents include carbonyldiimidazole, dicyclohexylcarbodiinri.de, pentofluorophenol trifluoroacetate, or 1- (3- dimethylaminopropyl) -3-ethylcarbodiimide. Exemplary inert solvents include ethers, including tetrahydrofuran and dioxane, N,N-dimethylformamide, acetonitrile, or methylene chloride.
Compounds of formula XI can be prepared from by the hydrolysis of compounds of formula XII using a hydroxide source. Exemplary hydroxide sources include sodium hydroxide or lithium hydroxide. Exemplary solvents include water, alcohols, and mixtures of ethers/water.
Compounds of formula XII can be prepared by treating a compound of formula XIII with a diazatizing reagent in an acidic aqueous medium. Sodium nitrite is an exemplary 15222
diazatizing reagent and dilute (IN) HC1 is an exemplary reaction solvent.
Compounds of formula XIII can be prepared via the reduction of a compound of formula XIV in an inert solvent. This reduction may, for example, be mediated via a platinum or palladium-catalyzed hydrogenation using platinum or palladium on carbon, hydrogen and an inert solvent such as ethanol or methanol or, alternatively, by use of a stoichiometric reducing agent, such as stannous(II) chloride, in an inert solvent such as ethyl acetate.
Compounds of formula XIV can be prepared by reacting compounds of formula XV with amines of the formula R3NH2. The reaction may be performed in an inert solvent as appropriate, such as ethanol, in the presence of appropriate base, such as triethylamine, and typically under elevated temperatures.
Compounds of formula XV can be prepared by reacting compounds of formula XVI with an amine, thiol or alcohol of the formula E-H. The reaction may be performed in an inert solvent as appropriate, such as N,N- dimethylformamide, in the presence of an appropriate base, such as triethylamine for amines and sodium hydride for thiols or alcohols. Methods of synthesis of compounds of formulas XVII, XVI, XV, XIV, XIII, XII, and XI are known to one skilled in the art. For example such methodology can be found in US 4,070,362, US 4,003,908, and US 4,048,182. Compounds of formula XVII are either commercially available or prepared by methods known to one skilled in the art. Scheme III
Figure imgf000024_0001
Figure imgf000024_0002
Compounds cf formula Ic, lie and IIIc [wherein E is Ei or E2 and X is Xi, X2 or X3, Y is C(R6) and Z is nitrogen] can be prepared via the aminolysis or esterification of a compound of formula XVIII using an appropriate carboxylic acid activating reagent and an appropriate amine or alcohol of the formula X-H in an inert solvent. Exemplary carboxylic acid activating agents include carbonyldiimidazole, dicyclohexylcarbodiimide, pentofluorophenol trifluoroacetate, or 1- (3-dimethylaminopropyl) -3- ethylcarbodiimide. Exemplary inert solvents include ethers, including tetrahydrofuran and dioxane, N,N- dimethylformamide, acetonitrile, or methylene chloride. Compounds of formula XVIII can be prepared from by the hydrolysis of compounds of formula XIX using a hydroxide source. Exemplary hydroxide sources include sodium hydroxide or lithium hydroxide. Exemplary solvents include water, alcohols, and mixtures of ethers/water.
Compounds of formula XIX can be prepared from the condensation of compounds of formula XIII with an activated ester derivative from an acid of the formula 15222
R6-C02H under basic conditions in an inert solvent typically under elevated temperatures. Exemplary activated esters include acid chlorides derived from R6- C02H, N,N-dialkylamide acetals (including, for example, N, N-dimethylformamide dimethyl acetal) and activated esters derived from the reaction of Rδ-C02H with exemplary carboxylic acid activating agents such as carbonyldiimidazole, dicyclohexylcarbodiiirri.de, pentofluorophenol trifluoroacetate, or l-(3- dimethylaminopropyl) -3-ethylcarbodiimide . Exemplary bases include sodium hydride, potassium hydride, cesium carbonate, potassium carbonate, potassium hexamethyldisilazide, and potassium t-butoxide. Exemplary inert solvents include ethers, N,N- dimethyIformamide, and acetonitrile.
Compounds of formula XIII are prepared as discussed in Scheme II.
Scheme IV
Figure imgf000025_0001
Compounds of formula la, Ila and Ilia [wherein E is Ei or E2, X is Xx, X2 or X3, Y is nitrogen, and Z is C(R7)] can also be prepared by reacting compounds of formula XX /15222
with an amine, thiol or alcohol of the formula E-H. The reaction may be performed in an inert solvent as appropriate, such as N,N-dimethylformamide, in the presence of an appropriate base, such as triethylamine for amines and sodium hydride for alcohols, and typically under elevated temperatures..
Compounds XX can be prepared by reacting compounds of formula XXI with an appropriate amine or alcohol of the formula X-H in an inert solvent in the presence of an appropriate base such as triethylamine. Exemplary inert solvents include ethers, including tetrahydrofuran and dioxane, N,N-dimethylformamide, acetonitrile, or methylene chloride.
Compounds XXI can be prepared from compounds of formula XXII by reacting with an appropriate dehydrating agent typically under elevated temperatures. Exemplary dehydrating agents include P0C13, PCI5, S0C12 and oxalyl chloride.
Compounds XXII can be prepared from by the hydrolysis of compounds of formula VII using a hydroxide source. Exemplary hydroxide sources include sodium hydroxide or lithium hydroxide. Exemplary solvents include water, alcohols, and mixtures of ethers/water.
Compound VII can be prepared as described in Scheme 1.
Preferred Compounds and Method
The following compounds of formula I and II are preferred:
Y is nitrogen. Z is nitrogen or C(R ).
Ei is -O-Ri, -NH-Ai-cycloalkyl, -NH-Ai-heterocyclo, or -NH-Ai-heteroaryl. 222
E2 is -NH-Ai-alkoxy, , -NH-A
Figure imgf000027_0001
substituted phenyl, or -NH-Aι-C02-alkyl . Ri is -Ai-substituted phenyl. Xi is -O-Ai-heteroaryl, -O-Ai-heterocyclo,
N A,— R,
-0-R9, -N(R9) (Rio),
R.
Figure imgf000027_0002
■N A, -R 25
X2 is -0-A2-heteroaryl, ζ
Figure imgf000027_0003
R7 is hydrogen.
R4 is hydrogen.
R3 is straight or branched chain alkyl of 1 to 4 carbons .
Rs is hydrogen, alkyl, -C02-alkyl, -Ai-phenyl, or -Ai-heteroaryl wherein alkyl is straight or branched chain of 1 to 4 carbons.
R2 is -(C=0)R9, -S-R9, -O-Rg, -N(R9) (Rιo), -NRnC02Ri9, - (C=0)N(Rn)CH2C02Ri9, -NRu (C=0) R19, -C02R9, nitrogen when A2 is alkynyl ending in a triple bond, -(C=0)N(Rι2) (R13) , phenyl, substituted phenyl, cycloalkyl, heterocyclo, heteroaryl, cycloalkyl -A3-phenyl, phenyl-A3-heteroaryl, heterocyclo-A3-phenyl, or heterocyclo-A3-heterocyclo.
R25 is -S-Rg, -NRiiC02Ri9/ nitrogen when A2 is alkynyl ending in a triple bond, - (C=0) N (Rn) CH2C02Ri9, /15222
Figure imgf000028_0001
-C02R9, - (C=0) N (R12) (Ri3) , phenyl, substituted phenyl, cycloalkyl, heterocyclo, heteroaryl, cycloalkyl-A3-phenyl, heterocyclo-A3-phenyl, phenyl-A3- heteroaryl or heterocyclo-A3-heterocyclo. Ai is an alkylene or substituted alkylene bridge of
1 to 6 carbons wherein said substituent is a straight or branched chain alkyl of 1 to 4 carbons.
A2 is a direct bond, an alkylene or substituted alkylene bridge of 1 to 6 carbons wherein said substituent is one or two members selected from alkyl, phenyl, substituted phenyl, -C02-alkyl, carboxy, hydroxy, -NH-(C=0) -alkyl, and -CH2- (C=0) -NH2, an alkenyl bridge of
2 to 4 carbons having one double bond, or an alkynyl bridge of 2 to 3 carbons having one triple bond wherein alkyl is straight or branched chain of 1 to 4 carbons.
The term "heterocyclo" in the preferred definitions refers to a substituted or unsubstituted fully saturated or partially saturated 5 to 7 membered monocyclic rings containing one or two heteroatoms selected from oxygen, sulfur and nitrogen and bicyclic rings wherein the monocyclic ring as defined above is fused to a phenyl cr substituted phenyl or wherein a bridge of 2 or 3 carbons is present between available carbon and nitrogen atom. The nitrogen and sulfur atoms may optionally be oxidized and the nitrogen atom may optionally be quaternized. The heterocyclo group may be attached at any available nitrogen or carbon atom. The heterocyclo ring may contain one or two substituents attached to an available carbon or nitrogen atom selected from alkyl, keto and -C02-alkyl, wherein alkyl is straight or branched chain of 1 to 4 carbons.
The term "heteroaryl" in the preferred definitions refers to a substituted or unsubstituted aromatic 5 or 6 membered monocyclic ring containing one or two oxygen or sulfur atoms and/or from one to four nitrogen atoms provided that the total number of heteroatoms in the ring is four or less, and bicyclic rings wherein the monocyclic ring as defined above is fused to a phenyl or substituted phenyl. The nitrogen and sulfur atoms may optionally be oxidized and the nitrogen atom may optionally be quaternized. The heteroaryl group may be attached at any available nitrogen or carbon atom. The heteroaryl ring may contain one or two substituents attached to an available carbon or nitrogen atom selected from straight or branched chain alkyl of 1 to 4 carbons and halo.
The term "cycloalkyl" in the preferred definitions refers to a fully saturated cyclic hydrocarbon group of 3 to 7 carbons and such cycloalkyl rings fused to a phenyl ring or such cycloalkyl rings 5 to 7 carbons having a carbon-carbon bridge of 3 or 4 carbons.
The term "substituted phenyl" refers to a phenyl ring having one, two, or three substituents selected from alkyl, halo, hydroxy, trifluoromethyl, alkoxy of 1 to 4 carbons, -N (alkyl) 2, and S02NH2 wherein alkyl is straight or branched chain of 1 to 4 carbons, and a phenyl ring substituted with a fused five membered ketal, i.e.
Figure imgf000029_0001
R9, Rio, Rn, Rι2, R13, Rι5, Rie and Rι9 are independently selected from the group consisting of . hydrogen, straight or branched chain alkyl of 1 to 4 carbons, phenyl, subsituted phenyl and -Ai-phenyl.
A3 is a direct bond, an alkylene bridge of 1 to 6 0
II carbons, or CH2-C- -N^
.s a 5 to 7 membered heterocyclo ring which can contain an additional nitrogen atom or can contain an oxygen or sulfur atom.
R2ι is attached to an available carbon or nitrogen atom and is hydrogen, straight or branched chain alkyl of 1 to 4 carbons, hydroxy or amino.
R22 is attached to an available carbon or nitrogen atom and is keto, -(C=0)R23, -C02R23, -NH- (C=0) -R23, -N (alkyl) 2, -Ai-hydroxy, -Ai-N (R9) (Rio) , -Ai-alkoxy, -A2- phenyl, -A2-substituted phenyl, or -A2-heteroaryl wherein alkyl is straight or branched chain of 1 to 4 carbons and alkoxy is such an alkyl bonded through an oxygen, n is one or two. m is zero or one. R23 is alkyl, -N(R9) (Rio), or -A2-heteroaryl wherein alkyl is straight or branched chain of 1 to 4 carbons.
-J3> represents a fused bicyclic ring wherein
the monocyclic ring ^— ^ is defined previously and
Figure imgf000030_0001
represents a substituted phenyl having two carbon
atoms in common with the monocyclic ring V— ' .
Figure imgf000030_0002
represents a spiro ring wherein the
monocyclic ring ^— is defined previously and — ^ represents a heterocyclo ring having a common carbon with
the monocyclic ring s—^ . /15222
Also preferred is the method of treating a cGMP- associated condition, particularly erectile dysfunction, with the preferred compounds of formulas I and II as defined above or with a compound of formula III wherein:
Y, Z, E2, R4 and R3 are as defined for the preferred compounds of formula II, and
Figure imgf000031_0001
—N R5, Ai A2, Rg, Rio, and ]" are as defined for (R2l)n the preferred compounds of formula II.
More Preferred Compounds And Method
The following compounds of formula I and II are more- preferred:
Y is nitrogen. Z is CH. R3 is ethyl.
R4 is hydrogen.
Ei is or -0-CH2- disubstituted
Figure imgf000031_0002
phenyl .
E2 is -NH-CH2-disubstituted phenyl, 0/15222
The term "disubstituted phenyl" refers to a phenyl ring having two substituents independently selected from halogen and methoxy or wherein said disubstituted phenyl
Figure imgf000032_0001
Xi is -O-Ai-heterocyclo, -O-Ai-heteroaryl, -NH-A2-R2,
Figure imgf000032_0002
X2 is -O-Ai-heterocyclo, -O-Ai-heteroaryl, -NH-A2-R25
Figure imgf000032_0003
Ai, A2,R2, R25, "heterocyclo" and "heteroaryl" are as defined in the preferred definitions.
Also more preferred is the method of treating a cGMP associated condition, particularly erectile dysfunction, with the more preferred compounds of formula I and II as defined above or with a compound of formula III wherein:
Y, Z, E2, R4 and R3 are as defined for the preferred compounds of formula II, and 0/15222
X3 is -NH-A-OR9 wherein A2 and Rg are as defined for the preferred compounds of formula II.
Most Preferred Compounds And Method
The following compounds of formulas I and II are most preferred :
Y is nitrogen . Z is CH . R3 is ethyl . R4 is hydrogen .
Figure imgf000033_0001
Xi and X2 are independently selected from the group consisting of
Figure imgf000033_0002
Figure imgf000034_0001
— HN- (CHJJJ-N
Figure imgf000034_0003
Figure imgf000034_0004
Figure imgf000034_0002
Figure imgf000034_0005
-NH— (CH2)3 — C02CH3 Also most preferred is the method of treating a cGMP associated condition, particularly erectile dysfunction, with the most preferred compounds of formulas I and II as defined above or with a compound of formula III wherein: Y, Z, E2, R4 and R3 are as defined the most preferred compounds of formula II, and
—NH-CH—CH,—OCH, X3 is I
C2H5
Utility
The compounds of the present invention inhibit cGMP PDE, and in particular are potent and selective inhibitors of cGMP PDE V. The present compounds are useful in the treatment of cGMP-associated conditions. A vcGMP-associated condition", as used herein, denotes a disorder which can be treated by inhibiting cGMP PDE or elevating the level of cGMP in a subject, wherein treatment comprises prevention, partial alleviation or cure of the disorder. Inhibition of cGMP PDE or elevation of the cGMP level may occur locally, for example, within certain tissues of the subject, or more extensively throughout a subject being treated for such a disorder. Treatment may be facilitated wherein elevation of the cGMP level potentiates additional beneficial therapeutic effects, such as where elevation of the cGMP level potentiates the effects of endothelium-derived. relaxing factor.
The compounds of the present invention are useful for the treatment of a variety of cardiovascular diseases including, but not limited to, hypertension, angina (stable, unstable, and variant) , (congestive) heart failure, restenosis, atherosclerosis, and dyslipidemia, as well as reduced blood vessel patency, thrombus, both venous and arterial, myocardial infarction, peripheral vascular disease, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, glaucoma, diseases characterized by disorders of gut motility, and forms of cancer responsive to the inhibition of cGMP PDE. In addition, these compounds are useful in the treatment of sexual dysfunction in both men (erectile dysfunction, for example, due to diabetes mellitus, spinal cord injury, radical prostatectomy, psychogenic etiology or any other cause) and women by improving blood flow to the genitalia, especially, the corpus cavernosum.
The present invention thus provides methods for the treatment of cGMP-associated conditions, comprising the step of administering to a subject in need thereof at least one compound of formulas I, II or III in an amount effective therefor. Other therapeutic agents such as those described below may be employed with the inventive compounds in the present methods. In the methods of the present invention, such other therapeutic agent (s) may be administered prior to, simultaneously with or following the administration of the compound (s) of the present invention.
The present invention also provides pharmaceutical compositions comprising at least one of the compounds of formulas I, II or III capable of treating a cGMP- associated condition in an amount effective therefor, and a pharmaceutically acceptable vehicle or diluent. The compositions of the present invention may contain other therapeutic agents as described below, and may be formulated, for example, by employing conventional solid or liquid vehicles or diluents, as well as pharmaceutical additives of a type appropriate to the mode of desired administration (for example, excipients, binders, preservatives, stabilizers, flavors, etc.) according to techniques such as those well known in the art of pharmaceutical formulation.
The compounds of formulas I, II and III may be administered by any suitable means, for example, orally, such as in the form of tablets, capsules, granules or powders; sublingually; bucally; parenterally, such as by subcutaneous, intravenous, intramuscular, or intrasternal injection or infusion techniques (e.g., as sterile injectable aqueous or non-aqueous solutions or suspensions) ; nasally such as by inhalation spray; topically, such as in the form of a cream or ointment; or rectally such as in the form of suppositories; in dosage unit formulations containing non-toxic, pharmaceutically acceptable vehicles or diluents. The present compounds may, for example, be administered in a form suitable for immediate release or extended release. Immediate release or extended release may be achieved by the use of suitable pharmaceutical compositions comprising the present compounds, or, particularly in the case of extended release, by the use of devices such as subcutaneous implants or osmotic pumps. The present compounds may also be administered liposomally.
Exemplary compositions for oral administration include suspensions which may contain, for example, microcrystalline cellulose for imparting bulk, alginic acid or sodium alginate as a suspending agent, methylcellulose as a viscosity enhancer, and sweeteners or flavoring agents such as those known in the art; and immediate release tablets which may contain, for example, microcrystalline cellulose, dicalcium phosphate, starch, magnesium stearate and/or lactose and/or other excipients, binders, extenders, disintegrants, diluents and lubricants such as those known in the art. The compounds of formulas I, II and III may also be delivered through the oral cavity by sublingual and/or buccal administration. Molded tablets, compressed tablets or freeze-dried tablets are exemplary forms which may be used. Exemplary compositions include those formulating the present compound (s) with fast dissolving diluents such as mannitol, lactose, sucrose and/or cyclodextrins. Also included in such formulations may be high molecular weight excipients such as celluloses (Avicel®) or polyethylene glycols (PEG) . Such formulations may also include an excipient to aid mucosal adhesion such as hydroxy propyl cellulose (HPC) , hydroxy propyl methyl cellulose (HPMC) , sodium carboxy methyl cellulose (SCMC) , maleic anhydride copolymer (e.g., Gantrez®) , and agents to control release such as polyacrylic copolymer (e.g., Carbopol® 934). Lubricants, glidants, flavors, coloring agents and stabilizers may also be added for ease of fabrication and use.
Exemplary compositions for nasal aerosol or inhalation administration include solutions in saline which may contain, for example, benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, and/or other solubilizing or dispersing agents such as those known in the art.
Exemplary compositions for parenteral administration include injectable solutions or suspensions which may contain, for example, suitable non- toxic, parenterally acceptable diluents or solvents, such as mannitol, 1, 3-butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid.
Exemplary compositions for rectal administration include suppositories which may contain, for example, a suitable non-irritating excipient, such as cocoa butter, synthetic glyceride esters or polyethylene glycols, which 222
are solid at ordinary temperatures, but liquefy and/or dissolve in the rectal cavity to release the drug.
Exemplary compositions for topical administration include a topical carrier such as Plastibase (mineral oil gelled with polyethylene) .
The effective amount of a compound of the present invention may be determined by one of ordinary skill in the art, and includes exemplary dosage amounts for an adult human of from about 0.05 to 100 mg/kg of body weight of active compound per day, which may be administered in a single dose or in the form of individual divided doses, such as from 1 to 4 times per day. It will be understood that the specific dose level and frequency cf dosage for any particular subject may be varied and will depend upon a variety of factors including the activity of the specific compound employed, the metabolic stability and length of action of that compound, the species, age, body weight, general health, sex and diet of the subject, the mode and time of administration, rate of excretion, drug combination, and severity of the particular condition. Preferred subjects for treatment include animals, most preferably mammalian species such as humans, and domestic animals such as dogs, cats, horses and the like, subject to cGMP- associated conditions.
The compounds of the present invention may be employed alone or in combination with each other and/or other suitable therapeutic agents useful in the treatment of cGMP-associated conditions such as other cGMP PDE inhibitors, particularly other cGMP PDE V inhibitors, prostanoids, α-adrenergic agonists, endothelin antagonists, angiotensin II (especially, subtype ATi) antagonists, angiotensin converting enzyme (ACE) inhibitors, renin inhibitors, and serotonin (5-HT2c) agonists. /15222
Exemplary such other therapeutic agents include the following: phentolamine, yohimbine, papaverine, apomorphine, sildenafil (see Drugs of the Future, 22, 138-143 (1997)), pyrazolopyrimidinones as described in U.S. Patent Nos. 5,272,147; 5,250,534; 5,426,107; and
5,346,901, quinazolinones as described in U.S. Patent No. 5,482,941; ATi antagonists selected from losartan, irbesartan, valsartan and candesartan; ETA antagonists selected from bosentan, ABT-627, and those described in U.S. Patent No. 5,612,359 and U.S. Patent Application Serial No. 60/035,832, filed January 30, 1997; PDE V inhibitors selected from imidazoquinazolines (see WO 98/08848), carbazoles (see WO 97/03675, WO 97/03985 and WO 95/19978), imidazopurinones (see WO 97/19947), benzimidazoles (see WO 97/24334), pyrazoloquinolines (see U.S. Patent No. 5,488,055), anthranilic acid derivatives (see WO 95/18097), fused heterocycles (see WO 98/07430) and thienopyrimidines (see DE 19632423) ; and 5-HT2c agonists selected from indoles (see J. Med. Chem . , 40, 2762-2769 (1997), EP 655440 and EP 657426).
The above other therapeutic agents, when employed in combination with the compounds of the present invention, may be used, for example, in those amounts indicated in the Physicians' Desk Reference (PDR) or as otherwise determined by one of ordinary skill in the art.
The following assay can be employed in ascertaining the degree of activity of a compound as a cGMP PDE inhibitor. Compounds described in the following Examples have been tested in this assay, and have shown activity. PDE Scintillation Proximity Assay Protocol
Sonicated human platelet homogenates are prepared by the method of Seiler, et al. (Seiler, S., Gillespie, E., Arnold, A.J., Brassard, C.L., Meanwell, N.A. and Fleming, J.S., " Imidazoquinoline derivatives: potent inhibitors of platelet cAMP phosphodiesterase which elevate cAMP levels and activate protein kinase in platelets," Thrombosis Research, 62: 31-42 (1991)). PDE V is abundant in human platelets, and accounts for approximately 90% of the cGMP hydrolytic activity in the homogenates. When necessary, PDE V can be resolved from other PDE activities in the homogenates by anion exchange chromatography on a fast protein liquid chromatography system (FPLC) using a Mono- Q anion exchange column (Pharmacia) eluted with a linear gradient of 10 mM - 450 mM NaCl.
The phosphodiesterase activity is assayed using a commercially available phosphodiesterase [3H]cGMP scintillation proximity (SPA) assay kit (Amersham) . The manufacturer's protocol is followed explicitly except that the reactions are carried out at room temperature and 3 mM nonradioactive cGMP is included in the suspension of SPA beads to prevent the synthesis of any additional radioactive products.
All documents cited in the present specification are incorporated herein by reference in their entirety. The following Examples illustrate embodiments of the present invention, and are not intended to limit the scope of the claims. Abbreviations
DMF = dimethylformamide
DMSO = dimethylsulfoxide Et - ethyl
HPLC = high pressure liquid chromatography
LRMS = low resolution mass spectrometry
Me = methyl
MeOH = methanol mp = melting point
THF = tetrahydrofuran tic = thin layer chromatography rt = room temperature h = hours H20 = water
P0C13 = phosphorus oxychloride
EtOH = ethanol
H3P04 = phosphoric acid
HC1 = hydrogen chloride NaOH = sodium hydroxide min = minutes
Et3N = triethylamine
Et20 = ethyl ether
EtOAc = ethyl acetate NaHC03 = sodium bicarbonate
MgS04 = magnesium sulfate
CH2C12 = methylene chloride
Na2S04 = sodium sulfate
K2C03 = potassium carbonate Preparation of Starting Materials
Preparation 1 Preparation of (1-Ethylpyrazol-5-yl-amino)methylenemalonate diethyl ester
Figure imgf000043_0001
A neat solution of 5-amino-1-ethylpyrazole (20.0 g, 180 mmol) and diethyl ethoxymethylenemaionate (42.8 g, 198 mmol) was heated at 120 °C for 5 h. This material was used directly without further purification. If needed, the product can be distilled at 154-160 °C (0.1 mm Hg) to afford the title compound as a liquid which solidified to afford the title compound as a pale colored solid: mp 50-53 °C.
Preparation 2 Preparation of 1-Ethyl-4-hydroxy-1H-pyrazolo[3.4-b]pyridine-5-carboxylic acid ethyl ester
Figure imgf000043_0002
(1-Ethylpyrazol-5-yl-amino)methylenemalonate diethyl ester (180 mmol from previous reaction) was dissolved in diphenyi ether (200 mL), and the resulting solution was placed in a preheated oil bath at 255 °C. The reaction solution was heated for 5 h, and then the diphenyi ether was removed via distillation. The resulting brown reaction mixture was cooled to room temperature and poured to hexane (1L). Cooling this solution to -78 °C, followed by filtration of the resulting precipitate afforded the title compound as a beige colored needle shaped solid that was >90% pure by HPLC and was used directly (25 g, 60% for 2 steps). A portion was recrystallized using ethanol-H2O to afford a white solid: mp 85-86 °C; LRMS (m/z) 236 (MH+). Preparation 3 Preparation of 4-Chloro-1-ethyl-1 H-pyrazolo[3,4-b]pyridine-5-carboxylic acid ethyl ester
Figure imgf000044_0001
1-Ethyl-4-hydroxy-1H-pyrazolo[3,4-b]pyridine-5-carboxyiic acid ethyl ester (15 g, 63.8 mmol) was dissolved in POCI3 (100 mL), and the resulting solution was heated at reflux for 4 h. The remaining POCI3 was removed via evaporation under reduced pressure. The residual light brown solid was recrystallized from EtOH-hexane to afford the title compound as a white solid (14 g, 55.3 mmol, 87%): HPLC (YMC S5 ODS 4.6x50 mm column, 4 minute gradient- 0% B to 100% B, 4 mL/min flow, solvent A: 10% MeOH-90% H2O- 0.2% H3PO4, solvent B: 90% MeOH-10% H2O-0.2% H3PO4) retention time 3.84 minutes showed a purity of 96%; LRMS (m/z) 254 (MH+).
Preparation 4
Preparation of 4-Chloro-1-ethyl-1 H-pyrazolo[3,4-b]pyridine-5-carboxylic acid ethyl ester
Figure imgf000044_0002
(1-Ethylpyrazo!-5-yl-amino)methylenemaionate diethyl ester (10.0 g, 42.6 mmol) was dissolved in 50 mL POCI3. This solution was heated at reflux for 10 h before the POCI3 was removed under reduced pressure. The resulting brown residue was diluted with 5 mL EtOH and extracted with hot hexane (200 mL x 3). The combined organic layers were evaporated under reduced pressure to afford the title compounds which formed light green needle shaped crystals upon standing at room temperature (5.4 g, 21.3 mmol, 50% yield). This material is identical to the one obtained in Preparation 3 (Η NMR, 13C NMR, MS, and HPLC).
Preparation 5 (3-Chloro-4-methoxyphenyl)methylamine hydrogen chloride
Figure imgf000045_0001
(4-Methoxyphenyl)methylamine (75.0 g, 0.55 mol) was dissolved in 400 mL diethyl ether. Hydrogen chloride (4.0 M in dioxane, 1.1 mol ) was added dropwise with vigorous stirring. After the addition completed, the white solid was filtered and washed thoroughly with diethyl ether. The solid was air dried over night (95.0 g, 100%).
Chlorine gas was bubbled into 400 mL glacial acetic acid with stirring until the weight gained equaled 7% of the starting acetic acid. In a 2 L round bottom flask, 4-methoxybenzylamine hydrogen chloride (32.0 g, 0.18 mol) was suspended in 400 mL glacial acetic acid with vigorous stirring. The chlorine solution (1.5 eq Cl2) was added in rapid drops in 30 min at room temperature. The resulted suspension was stirred for another 20 min before N2 was bubbled in to remove Cl2 and HCI into a 6 N NaOH trap. The acetic acid was evaporated under reduced pressure to 100 mL. To this white slurry, diethyl ether (300 mL) was used to loosen the solid which was then filtered. The solid was resuspended with 50 mL acetic acid followed by the addition of 50 mL diethyl ether and filtration. This process was repeated twice. The white solid was transferred to a 1 L Erhlenmeyer flask and suspended in 400 mL THF. This suspension was heated to boiling for 10 min before filtration. The undissolved solid was filtered, and twice resuspended in boiling THF (100 mL) with filtration to afford the title compound (27.0 g, 71%) as a white solid. This material contained <2% starting material and <2% dichlorinated material. Preparation 6 General Procedure to Prepare 4-Aminopyrazolopyridine-5-carboxylic acids
Figure imgf000046_0001
The appropriate 4-chloro-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid ethyl ester ( 4.0 mmol) and the appropriate amine (4.4 mmol)) were suspended in EtOH (20 mL). To this suspension was added of Et3N (2.8 mL, 20 mmol), and the resulting solution was heated at reflux for 10 h. To the resulting suspension was added 6 N NaOH (2.7 mL, 16 mmol), and the resulting mixture was heated at reflux for 3 h. EtOH was then removed via evaporation under reduced pressure, and the residual white solid was dissolved in 25 mL 0.1 N NaOH. The resulting aqueous mixture was extracted with diethyl ether (3x150 mL), and the organic extracts were discarded. The aqueous layer was acidified with 1 N HCI, and the resulting white precipitate was collected by filtration and washed sequentially with 1 N HCI, H2O, EtOH and Et2O to afford the desired 4-aminopyrazolopyridine.
Using the above described procedure, the following compounds were prepared:
A. 4-[[(3-Chloro-4-methoxyphenyi)methyl]amino]-1 -ethyl- 1 H- pyrazolo[3,4-b]pyridine-5-carboxylic acid
Figure imgf000046_0002
4-Chloro-1-ethyl-1 H-pyrazolo[3,4-b]pyridine-5-carboxyiic acid ethyl ester and (3-chloro-4-methoxyphenyl)methylamine was used to afford the title compound (87%) as a white solid: mp 250 °C (decomposed).
B. (R)-4-[(1 -Cyclohexylethyi)amino]-1 -ethyl-1 H-pyrazolo[3,4- b]pyridine-5-carboxylic acid
Figure imgf000047_0001
4-Chloro-1-ethyl-1 H-pyrazolo[3,4-b]pyridine-5-carboxylic acid ethyl ester and (R)-2-cyclohexylethylamine was used to afford the title compound (80%o) as a white solid: mp 200 °C (decomposed).
C. 4-[[(3,4-Dioxomethylenephenyl)methyl]amino]-1 -ethyl-1 H- pyrazolo[3,4-b]pyridine-5-carboxylic acid
Figure imgf000047_0002
4-Chloro-1 -ethyl-1 H-pyrazolo[3,4-b]pyridine-5-carboxylic acid ethyl ester and (3,4-dioxomethylenephenyl)methylamine were used to afford the title compound (80%) as a white solid: mp 230 °C (decomposed). Preparation 7
(R)-4-[(1 -Cyclohexylethyl)amino]-1 -ethyl-1 H-pyrazolo[3,4-blpyridine-5- carboxylic acid pentafluorophenyl ester
Figure imgf000048_0001
The (R)-4-[(1 -Cyclohexylethyl)amino]-1 -ethyl-1 H-pyrazolo[3,4- b]pyridine-5-carboxyiic acid (0.3 g, 0.95 mmol) was suspended in 2 mL DMF with the subsequent addition of pyridine (0.15 g, 1.9 mmol), pentofiuorophenol trifluoroacetate ( 0.53 g, 1.9 mmol), and a catalytic amount of pentafluorophenol (10 mg). This mixture was stirred at rt for 48 h, and the resulting mixture was diluted with EtOAc (50 mL) and washed with 0.1 N HCI (50 mL), 5% NaHCO3 (3 x 50 mL), and brine (5 x 50 mL). The organic extract was dried over MgSO4 and concentrated to afford the title compound as a viscous oil (0.44 g, 95%): HPLC (YMC S5 ODS 4.6x50 mm column, 4 minute gradient- 50% B to 100% B, 4.0 mL min flow, solvent A: 10% MeOH-90% H2O-0.2% H3PO4, solvent B: 90% MeOH-10% H2O-0.2% H3PO4) retention time 5.06 minutes showed a purity of 95%; LRMS (m/z) 483 (MH+).
The resulting product contained ~5% free pentafluorophenol and was used directly in subsequent reactions.
Preparation 8
Preparation of 1-Ethyl-4-hydroxy-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid
Figure imgf000048_0002
1-Ethyl-4-hydroxy-1 H-pyrazoIo[3,4-b]pyridine-5-carboxylic acid ethyl ester (10.0 g, 42.6 mmol) was suspended in 50 mL EtOH. To this suspension was added 6 N NaOH (21.3 mL, 3 eq). The resulting light brown solution was heated at reflux for 3 hr before being diluted with 100 mL water. The aqueous solution was extracted with diethyl ether (50 mL x 3) and the organic layer was discarded. The aqueous layer was acidified with 1 N HCI and a white precipitate formed. The title compound was collected by filtration and rinsed with water and diethyl ether affording a white solid (7.9 g, 90%): mp 218 °C (decomposed); LRMS (m/z) 208 (MH+).
Preparation 9 4-Chloro-1 -ethyl-1 H-pyrazolo[3,4-b]pyridine-5-carboxylic acid chloride
Figure imgf000049_0001
1-Ethyl-4-hydroxy-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid (0.92 g, 4.4 mmol) was dissolved in 10 mL POCI3, and the solution was heated at reflux for 2 hr. The excess POCI3 was removed under reduced pressure and azeotroped three times with toluene. The brown residue was dissolved in 20 mL CH2CI2, Et3N (5 eq) and an amine such as 4-aminomethylpyridine (1.2 eq) were then added sequentially. The resulting solution was stirred at rt for 2 h before being diluted with 100 mL water. The organic layer was separated and the aqueous layer was extracted with CH2CI2 (50 mL X 3). The combined organic layer was washed with NaHCO3 and water. The organic layer was dried over Na2SO4 and the solvent was removed. The residue was chromatographed using silica gel column (5% MeOH-CH2CI2) to afford the title compound as a white solid (0.6 g, 44%). 15222
Example 1
General Procedures for the Preparation of 4-Aminopyrazolopyridines-5- carboxylates and 4-Aminopyrazolopyridines-5-carboxamides
Figure imgf000050_0001
Method A: To a mixture of the appropriate 4-amino pyrazolopyridine-
5-carboxylic acid (5.6 mmol), 1-[3-(dimethylamino)propylJ-3-ethylcarbodiimide hydrochloride (EDAC* HCI, 1.6 g, 8.4 mmol), and 1 -hydroxybenzotriazole (1.1 g, 8.4 mmol) in anhydrous THF (50 mL) was added triethylamine (4.0 mL, 28.0 mmol), and the resulting solution was stirred at rt for 10 min. To the resulting reaction solution was then added the appropriate amine or alcohol of the formula X-H (6.7 mmol, 1.2 eq), and this solution was stirred at rt for 24 h. The resulting reaction solution was concentrated via evaporation under reduced pressure, and the residual solid was resuspended in EtOAc (250 mL). This EtOAc suspension was washed with H2O (200 mL), NaOH (0.1 N, 2 x 200 mL), potassium phosphate buffer (50 mM, pH 7, 2 x 200 mL) and H2O (200 mL). The organic layer was then dried over anhydrous Na2SO4 and concentrated via evaporation under reduced pressure until a solid began to precipitate. This mixture was allowed to stand at rt, and the precipitated solid was collected and washed thoroughly with Et2O and once with 50% Et2O- EtOAc to afford the appropriate title compound as a solid. A second crop may be obtained from the mother liquors. If a solid is not obtained by this method, then purification of the extraction residue via column chromatography using silica gel and elution with 5% CH2CI2-CH3OH afforded the appropriate title compound.
Method B: The appropriate 4-amino pyrazolopyridine-5-carboxylic acid (2.8 mmol) was suspended in anhydrous CH2Cl2 (20 mL), and oxalyl chloride (0.72 mL, 8.4 mmol) was added to this mixture followed by 2 drops of DMF. The resulting suspension was stirred at rt for 1 h after the suspension cleared. The resulting reaction solution was evaporated under reduced pressure, and the residue was redissolved in anhydrous CH2CI2 (20 mL). To this solution was added sequentially Et3N (1.9 mL, 14.0 mmol) and then the appropriate amine or alcohol of the formula X-H (4.2 mmol). The solution was stirred at rt for 2 hr. The solvent was removed and the resulted residue was suspended in 100 mL EtOAc. The organic was subsequently washed with water, 1 N NaOH, and 1 N HCI. The organic layer was then dried over anhydrous Na2S04 and concentrated via evaporation under reduced pressure. The resulted crude products have been purified by either crystallization (>100 mg scale) or preparative HPLC (<100 mg scale).
Method C: The appropriate 4-amino-1 -ethyl-1 H-pyrazolo[3,4- b]pyridine-5-carboxylic acid pentafluorophenyl ester (0.16 mmol) was dissolved in anhydrous THF (2 mL) and triethylamine (49 mg, 0.48 mmol). The appropriate amine or alcohol of the formula X-H (0.32 mmol, 2.0 eq) was then added, and the reaction solution was stirred at rt for 12 h. The resulting reaction solution was diluted with EtOAc (5 mL), and the organic layer was washed with 0.1 N NaOH (5 mL), potassium phosphate buffer (pH 7) and brine. The organic layer was then dried over anhydrous Na2SO4 and concentrated via evaporation under reduced pressure to afford the title compound. Purification of the crude product if necessary can be accomplished by either crystallization or preparative HPLC.
Compounds listed in the following Tables prepared by these procedures are denoted as methods 1A, 1B, or 1C.
^9- Example 2
General Procedure for the Preparation of 4-Aminopyrazolopyridines-5- carboxylates and 4-Aminopyrazolopyridines-5-carboxamides
Figure imgf000052_0001
To a solution of the appropriate 4-chloro-1 H-pyrazolo[3,4-b]pyridine-5- carboxyiic acid chloride (4.4 mmol) in CH2CI2 was added the appropriate amine or alcohol of the formula X-H (6.6 mmol) and Et3N (22.0 mmol) at 0 °C. The reaction was stirred from 0 °C to rt until completion. The solvent was removed via evaporation under reduced pressure, and the residue was purified by silica gel chromatography.
To a solution of the resulting 4-chloropyrazolopyridines-5-carboxamide or ester (0.21 mmol) in ethanol (5 mL) was added an appropriate amine of the formula E-H (0.24 mmol) and Et3N (1.4 mmol). The solution was heated at reflux for 2 hr before being diluted with CH2CI2. The organic layer was sequentially washed with 1N HCI, and water, dried, and evaporated under reduced pressure. The residue was purified by silica gel chromatography to afford the title compound.
Compounds listed in the following Tables prepared by this procedure are denoted as method 2.
Example 3
General Procedure for the Preparation of 4-Oxypyrazolopyridines-5- carboxylates and 4-Oxypyrazolopyridines-5-carboxamides
Figure imgf000052_0002
To a solution of the appropriate 4-chloro-1H-pyrazolo[3,4-b]pyridine-5- carboxylic acid chloride (4.4 mmol) in CH2CI2 was added the appropriate amine or alcohol of the formula XrH (6.6 mmol) and Et3N (22.0 mmol) at 0 °C. The reaction was stirred from 0 °C to rt until completion. The solvent was removed via evaporation under reduced pressure, and the residue was purified by silica gel chromatography.
To a solution of the resulting 4-chloropyrazolopyridine-5-carboxamide or ester (0.21 mmol) in DMF (2 mL) was added an appropriate alcohol of the formula RrOH (0.24 mmol) and K2CO3 (0.42 mmol). The mixture was stirred at 50 °C for 2 hr before being diluted with CH2CI2. The organic layer was sequentially washed with 1 N HCI, and water, dried, and evaporated under reduced pressure. The residue was purified by silica gel chromatography to afford the title compound.
Compounds listed in the following Tables prepared by this procedure are denoted as method 3.
Example 4 General Procedure for the Preparation of 4-Thiopyrazolopyridines-5- carboxylates and 4-Thiopyrazolopyridines-5-carboxamides
Figure imgf000053_0001
To a solution of the appropriate 4-chloro-1H-pyrazolo[3,4-b]pyridine-5- carboxylic acid chloride (4.4 mmol) in CH2CI2 was added the appropriate amine or alcohol of the formula XrH (6.6 mmol) and Et3N (22.0 mmol) at 0 °C. The reaction was stirred from 0 °C to rt until completion. The solvent was removed via evaporation under reduced pressure, and the residue was purified by silica gel chromatography. To a solution of the resulting 4-chloropyrazolopyridine-5-carboxamide or ester (0.21 mmol) in DMF (2 mL) was added an appropriate thiol of the formula RrSH (0.24 mmol) and K2CO3 (0.42 mmol). The mixture was stirred at 50 °C for 2 hr before being diluted with CH2CI2. The organic layer was sequentially washed with 1 N HCI, and water, dried, and evaporated under reduced pressure. The residue was purified by silica gel chromatography to afford the title compound.
Example 5 Preparation of 4-[[(3-Chloro-4-methoxyphenyl)methyl]amino]-
1-ethyl-N-(4-pyridinylmethyl)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
Figure imgf000054_0001
Using the procedure of method 1A, triethylamine (4.0 ml, 28. 0 mmol) was added to a mixture of 4-[[(3-chloro-4-methoxyphenyl)methyl]amino]-1- ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid (2.0 g, 5.6 mmol), 1-[3- (dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (EDAC»HCI, 1.6 g, 8.4 mmol), and 1-hydroxybenzotriazole (1.1 g, 8.4 mmol) in anhydrous THF (50 ml). The resulting solution was stirred at rt for 10 min. To the resulting reaction solution was then added 4-aminomethylpyridine (0.72 g, 6.7 mmol) and this solution was stirred at rt for 24 h. The resulting reaction solution was concentrated via evaporation under reduced pressure, and the residual solid was resuspended in EtOAc (250 mL). This EtOAc suspension was washed with H2O (200 ml), NaOH (0.1 N, 2 x 200 ml), potassium phosphate buffer (50 mM, pH 7, 2 x 200 ml) and H2O (200 mL). The organic layer was then dried over anhydrous Na2SO4 and concentrated via evaporation under reduced pressure until a solid began to precipitate. This mixture was allowed to stand at rt, and the precipitated solid was collected and washed thoroughly with Et2O and once with 50% Et2O-EtOAc to afford a white solid (2.2 g, 90%): mp: 209-210.5 C; LRMS (m/z) 450; 13C NMR (CDCI3): δ 171.8, 156.4, 153.7, 153.1 , 151.7, 150.8, 150.5, 135.7, 132.0, 130.5, 128.3, 124.5, 124.3, 114.2, 105.6, 104.4, 57.4, 49.8, 43.7, 43.6, 15.9. Anal. Calc'd for C23H23C1N6O2: C, 61.26; H, 5.14; N, 18.64; Cl, 7.86. Found: C, 61.03; H, 5.22; N, 18.66; Cl, 8.02.
Table
The following compounds of formula I were prepared wherein R3 is
ethyl, R is hydrogen, Y is nitrogen, Z is CH, and Ei is
Figure imgf000056_0001
Figure imgf000056_0002
22
Figure imgf000057_0001
Figure imgf000058_0001
Figure imgf000059_0001
22
Figure imgf000060_0001
Figure imgf000061_0001
222
Figure imgf000062_0001
22
Figure imgf000063_0001
222
Figure imgf000064_0001
Figure imgf000065_0001
Figure imgf000066_0001
0/15222
Figure imgf000067_0001
0/15222
Figure imgf000068_0001
Figure imgf000069_0002
The following compounds of formula I were prepared wherein R3 is
Figure imgf000069_0001
Figure imgf000069_0003
Figure imgf000070_0001
00/15222
The following compounds of formula I were prepared wherein R3 is ethyl, R4 is hydrogen, Y is nitrogen, and Z is CH:
Figure imgf000071_0001
0/15222
The following compounds of formula II were prepared wherein R3 is ethyl, R is hydrogen, Y is nitrogen, Z is CH, and E2 is
Figure imgf000072_0001
Figure imgf000072_0002
0/15222
Figure imgf000073_0001
Figure imgf000074_0001
0/15222
Figure imgf000075_0001
Figure imgf000076_0001
0/15222
Figure imgf000077_0001
Figure imgf000078_0001
0/15222
Figure imgf000079_0001
Figure imgf000080_0001
00/15222
Figure imgf000081_0001
00/15222
Figure imgf000082_0001
0/15222
Figure imgf000083_0001
00/15222
The following compounds of formula II were prepared wherein R3 is ethyl, R4 is hydrogen, Y is nitrogen, and Z is CH2:
Figure imgf000084_0001
Figure imgf000085_0001
00/15222
The following compounds of formula III were prepared wherein R3 is ethyl, R4 is hydrogen, Y is nitrogen, Z is CH2, and Ei is
Figure imgf000086_0001
Figure imgf000086_0002
Figure imgf000087_0001
/15222
Figure imgf000088_0001
Figure imgf000089_0001

Claims

What is claimed is:
1. A compound of the formulas
(I)
(Hi
Figure imgf000090_0001
including a pharmaceutically acceptable salt thereof wherein:
Ex is -O-Ri, -S-Ri, -NH-Ai-cycloalkyl, -NH- Aχ-substituted cycloalkyl, -NH-Ai-heterocyclo, or -NH-Ai-heteroaryl ;
E2 is -NH-Ai-alkoxy, -NH-Ai-C02alkyl,
, -NH-Ai-aryl, or -NH-Ai-substituted
Figure imgf000090_0002
aryl;
Ri is -Ai-cycloalkyl, -Ai-subsituted cycloalkyl, -Aι~
R 15
"An -N alkoxy, \ -Ai-aryl, -Ai-substituted aryl,
R 16 -Ai-heterocyclo, or -Ai-heteroaryl; 00/15222
Xi is -0-Aι-R2, -0-R9, -N(R9) (Rι0) ,
Figure imgf000091_0001
monocylic ring R 2222) I m a fused bicyclic ring
Figure imgf000091_0002
Figure imgf000091_0003
-N A, -R 25
X2 is -0-A;-R25, κ5 a monocyclic ring
m ,
Figure imgf000091_0004
or a spiro ring
Figure imgf000091_0005
-N-A2—0-R9
X3 is -O-Rg, -O-Aχ-0-Rg, -N(R9) (R10), I
Rc
'
Figure imgf000091_0006
Ai is an alkylene or substituted alkylene bridge of 1 to 10 carbons;
Y is nitrogen or C(Rε);
Z is nitrogen or C(R7) with the proviso that at least one of Y or Z is nitrogen;
R3 is hydrogen, alkyl, cycloalkyl, substituted cycloalkyl, substituted alkyl, -Aχ-aryl, -Ai-substituted aryl, -Ai-cycloalkyl, or -Ai-substituted cycloalkyl; Re and R are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, -Ai-cycloalkyl, -Ai-substituted cycloalkyl, -Ai-aryl, Ai-substituted aryl, -Ai-heterocyclo, and Ai-heteroaryl;
R4 is hydrogen, -N(Ri2) (Ri3) , -OR12 or 1- or 3- i idazolyl;
A2 is a direct bond, an alkylene or substituted alkylene bridge of 1 to 10 carbons, an alkenyl or substituted alkenyl bridge of 2 to 10 carbons having one or more double bonds, or an alkynyl or substituted alkynyl bridge of 2 to 10 carbons having one or more triple bonds;
R2 is cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, heteroaryl, cycloalkyl-A3- cycloalkyl, cycloalkyl-A3-substituted cycloalkyl, cycloalkyl-A3-aryl, cycloalkyl-A3-substituted aryl, cycloalkyl-A3-heterocyclo, cycloalkyl-A3-heteroaryl, substituted cycloalkyl~A3-cycloalkyl, substituted cycloalkyl-A3-substituted cycloalkyl, substituted cycloalkyl-A3-aryl, substituted cycloalkyl-A3-substituted aryl, substituted cycloalkyl-A3-heterocyclo, substituted cycloalkyl-A3-heteroaryl, aryl-A3-cycloalkyl, aryl-A- substituted cycloalkyl, aryl-A3-aryl, aryl-A3-substituted aryl, aryl-A3-heterocyclo, aryl-A3-heteroaryl, substituted aryl-A3-cycloalkyl, substituted aryl-A3-substituted cycloalkyl, substituted aryl-A3-aryl, substituted aryl-A3- substituted aryl, substituted aryl-A3-heterocyclo, substituted aryl-A3-heteroaryl, heterocyclo-A3-cycloalkyl, heterocyclo-A3-substituted cycloalkyl, heterocyclo-A3- aryl, heterocyclo-A3-substituted aryl, heterocyclo-A3- heterocyclo, heterocyclo-A3-heteroaryl, heteroaryl-A3- cycloalkyl, heteroaryl-A3-substituted cycloalkyl, heteroaryl-A3-aryl, heteroaryl-A3-heterocyclo, heteroaryl- A3-heteroaryl, cyano, -OR9, -SR9, -(C=0)R9, -N(R9)(Rι0), -C02R9, -(C=0)N(R12) (R13) , -S02N(R12) (R13), -NRn (OO) Rι9, -NRn(C=0)N(Rι2) (R13) , -O- (C=0) N (Ri2) (Ri3) provided that A2 is not a direct bond, -NRnC02Rι9, - (C=0) N (Ru) CHC02Rι9, nitrogen v/hen A2 is alkynyl ending in a triple bond, or NH when A2 is alkenyl ending in a double bond;
R25 is cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, heteroaryl, cycloalkyl-A3- cycloalkyl, cycloalkyl-A3-substituted cycloalkyl, cycloalkyi-A3-aryl, cycloalkyl-A3-substituted aryl, cycloalkyi-A3-heterocyclo, cycloalkyl-A3-heteroaryl, subsituted cycloalkyl-A3-cycioalkyl, substituted cycloalkyl-A3-substituted cycloalkyl, substituted cycloalkyl-A3-aryl, substituted cycloalkyl-A3-substituted aryl, substituted cycloalkyl-A3-heterocyclo, substituted cycloalkyi-A3-heteroaryl , aryl-A3~cycioaikyl, aryl-A3- substituted cycloalkyl, aryi-A3-aryl, aryl-A3-substir.uted aryl, aryl-A3-heterocyclo, aryl-A3-heteroaryi, substituted aryl-A3-cycloalkyl, substituted aryl-A3-substituted cycloalkyl, substituted aryi-A3-aryl, substituted aryl-A3-substituted aryl, substituted aryl-A3- heterocyclo, substituted aryl-A3-heteroaryl, heterocyclo- A3-cycloalkyl, heterocyclo-A3-substituted cycloalkyl, heterocycio-A3-aryl, heterocyclo-A3-suDStituted aryl, heterocycio-A3-heterocyclo, heterocyclo-A3-heteroaryl, heteroaryl-A3-cycloalkyl, heteroaryl-A3-substituted cycloalkyl, heteroaryl-A3-aryl, heteroaryl-A3-substituted aryl, heteroaryl-A3-heterocyclo, heteroaryl-A3-heteroaryl, cyano, -S-R9, -(C=0)Rn, -C02Rι9, - (C=0) N (R12) (Ri3) , -S02N(Rι2)R13),-NR9(C=0)Rιo, -NRU (C=0) N (R12) (R13) , -0- (C=0)N (Ri2) (Rι3) provided that A2 is not a direct bond, -NRuC02Rι9, " (C=0)N(Ru) CH2C02Ri9, nitrogen when A2 is alkynyl ending in a triple bond, or NH when A2 is alkenyl ending in a double bond;
A3 is a direct bond, an alkylene or substituted alkylene bridge of 1 to 10 carbons, an alkenyl or substituted alkenyl bridge of 2 to 10 having one or more double bonds, an alkynyl or substituted alkynyl bridge of 2 to 10 carbons having one or more triple bonds, -(CH2)d-0-(CH2)e-, -(CH2)d-S-(CH2)e-, or -(CH2)d-(C=0)-(CH2)e-; d is zero or an integer from 1 to 6; e is zero or an integer from 1 to 6;
R5 is hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, aryl, -Aχ-aryl, substituted aryl, -Aj-substituted aryl, heterocyclo, -Ai- heterccyclo, heteroaryl or -A-heteroaryl;
Rs, R10, Rn, R12, 13, i5, i6, and Ri9 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo, heteroaryl, -Ai-cycloalkyl, -Ax-substituted cycloalkyl, -Ai-aryl, -Ai-subsituted aryl, -Ai-heterocyclo and -Ai-heteroaryl, or Ri2 and Ri3 taken together with the N atom to which they are attached represent a heterocyclo ring;
Vy— represents a monocyclic heterocyclo or heteroaryl ring of 4 to 8 atoms containing up to 3 additional heteroatoms (up to 2 additional heteroatoms when the ring is 4 atoms) which are selected from one or two oxygen atoms and/or one or two sulfur atoms and/or one, two or three nitrogen atoms;
R2ι is attached to an available carbon or nitrogen atom and is hydrogen, alkyl, halogen, hydroxy, trifluoromethyl, amino, alkoxy or carboxy; R22 is attached to an available carbon or nitrogen atom and is keto, -(C=0)R23, -C02-R23, -NH- (C=0) -R23, -N (alkyl) 2, -Ax-hydroxy, -Ai-N (R9) (Rio) , -Ai-alkoxy, -Ai- carboxy, -A2-cycloalkyl, -A2-substituted cycloalkyl, -A2- aryl, -A2-substituted aryl, 0/15222
-A2-heterocyclo, or -A-heteroaryl; n is one or two; m is zero or one;
R23 is alkyl, -N(R9) (R10), -Ai-hydroxy, -Ai-N (R9) (Ri0) , -Ax-carboxy, -A2-cycloalkyl, -A2-substituted cycloalkyl, -A2-aryl, -A2-substituted aryl, -A2-heterocyclo or -A2-heteroaryl ;
Figure imgf000095_0001
represents a fused bicyclic ring wherein
the monocyclic ring -o ,is defined previously and
Figure imgf000095_0002
represents a cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo or heteroaryl having two carbon atoms in common with the monocyclic ring
Figure imgf000095_0003
represents a spiro ring wherein the
Figure imgf000095_0004
monocyclic ring iss ddeeffiinned previously and represents a cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heterocyclo or heteroaryl ring having a
common carbon with the monocyclic ring -o
2. A compound of Claim 1 including a pharmaceutically acceptable salt thereof wherein:
Y is nitrogen;
Z is nitrogen or C(R7);
Ei is -O-Ri, -NH-Ai-cycloalkyl, -NH-Ai-heterocyclo, or -NH-Ai-heteroaryl; 0/15222
E2 is -NH-Ai-alkoxy, -NH-Ai-
Figure imgf000096_0001
substituted phenyl, or -NH-Ai-C02-alkyl; Ri is -Ai-substituted phenyl; Xi is -O-Ai-heteroaryl, -O-Ai-heterocyclo,
Figure imgf000096_0002
■N- -A-—R,
X2 is -0-A2-heteroaryl, Rκ
Figure imgf000096_0003
R7 is hydrogen; R4 is hydrogen;
R3 is straight or branched chain alkyl of 1 to 4 carbons;
R5 is hydrogen, alkyl, -C02-alkyl, -Ai-phenyl, or -Ai-heteroaryl wherein alkyl is straight or branched chain of 1 to 4 carbons;
R2 is -(C=0)R9, -S-R9, -0-R9, -N(R9) (Rιo), -NRnC02Ri9, - (C=0)N(Rn)CH2C02Ri9, -NRu (C=0) Ri9, -C02R9, nitrogen when A2 is alkynyl ending in a triple bond, - (C=0) N (Ri2) (RX3) , phenyl, substituted phenyl, cycloalkyl, heterocyclo, heteroaryl, cycloalkyl-A3-phenyl, phenyl-A3-heteroaryl, heterocyclo-A3-phenyl, or heterocyclo-A3-heterocyclo; 00/15222 rL 1,u
R25 is -S-Rg, -NRnC02Ri9, nitrogen when A2 is alkynyl ending in a triple bond, - (C=0) N (Rn) CH2C02Rι9 -NRιι(C=0)Ri9, -C02R9, - (C=0) N (R12) (R13) , phenyl, substituted phenyl, cycloalkyl, heterocyclo, heteroaryl, cycloalkyl-A3-phenyl, heterocyclo-A3-phenyl, phenyl-A3- heteroaryl or heterocyclo-A3-heterocyclo;
Ai is an alkylene or substituted alkylene bridge of 1 to 6 carbons wherein said substituent is a straight or branched chain alkyl of 1 to 4 carbons; A2 is a direct bond, an alkylene or substituted alkylene bridge of 1 to 6 carbons wherein said substituent is one or two members selected from alkyl, phenyl, substituted phenyl, -C0-alkyi, carboxy, hydroxy, -NH- (C=0) -alkyl, and -CH2- (C=0) -NH2, an alkenyl bridge of 2 to 4 carbons having one double bond, or an alkynyl bridge of 2 to 3 carbons having one triple bond wherein alkyl is straight or branched chain of 1 to 4 carbons; the term "heterocyclo" refers to a substituted or unsubstituted fully saturated or partially saturated 5 to 7 membered monocyclic rings containing one or two heteroatoms selected from oxygen, sulfur and nitrogen and bicyclic rings wherein the monocyclic ring as defined above is fused to a phenyl cr substituted phenyl or wherein a bridge of 2 or 3 carbons is present between available carbon and nitrogen atom, said nitrogen and sulfur atoms may optionally be oxidized and said nitrogen atom may optionally be quaternized; said heterocyclo group may be attached at any available nitrogen or carbon atom, and said heterocyclo ring may contain one or two substituents attached to an available carbon or nitrogen atom selected from alkyl, keto and -C02-alkyl, wherein alkyl is straight or branched chain of 1 to 4 carbons; the term "heteroaryl" refers to a substituted or unsubstituted aromatic 5 or 6 membered monocyclic ring containing one or two oxygen or sulfur atoms and/or from one to four nitrogen atoms provided that the total number of heteroatoms in the ring is four or less, and bicyclic rings wherein the monocyclic ring as defined above is fused to a phenyl or substituted phenyl, said nitrogen and sulfur atoms may optionally be oxidized and said nitrogen atom may optionally be quaternized, said heteroaryl group may be attached at any available nitrogen or carbon atom, and said heteroaryl ring may contain one or two substituents attached to an available carbon or nitrogen atom selected from straight or branched chain alkyl of 1 to 4 carbons and halo; the term "cycloalkyl" refers to a fully saturated cyclic hydrocarbon group of 3 tc ~ :arbons and such cycloalkyl rings fused to a phenyl ring or such cycloalkyl rings 5 tc 7 carbons naving a carbon-carbon bridge of 3 or 4 carbons; the term "substituted phenyl" refers to a phenyl ring having one, two, or three substituents selected from alkyl, halo, hydroxy, trifluoromethyl, alkoxy of 1 to 4 carbons, -N (alkyl) 2, and S02NH2 wherein alkyl is straight or branched chain of 1 to 4 carbons, and a phenyl ring substituted with a fused five membered ketal;
R5, Rio, Rn, Rn, Rι3, Rι5, Rι6 and Rι9 are independently selected from the group consisting of hydrogen, straight or branched chain alkyl of 1 to 4 carbons, phenyl, subsituted phenyl and -Ai-phenyl;
A3 is a direct bond, an alkylene bridge of 1 to 6 0
II carbons, or 2 ;
■ov— ' is a 5 to 7 membered heterocyclo ring which can contain an additional nitrogen atom or can contain an oxygen or sulfur atom; R21 is attached to an available carbon or nit αen atom and is hydrogen, straight or branched chain ■ -yl of 1 to 4 carbons, hydroxy or amino;
R22 is attached to an available carbon or nit ;en atom and is keto, -(C=0)R23, -C02R23, -NH- (C=0) -R23,
-N(alkyl)2, -Ai-hydroxy, -Aχ-N (Rg) (Ri0) , -Ai-alkoxy, -A2- phenyl, -A2-substituted phenyl, or -A2-heteroaryl v trein alkyl is straight or branched chain of 1 to 4 carb -~.s and alkoxy is such an alkyl bonded through an oxygen; n is one or two; m is zero or one;
R23 is alkyl, -N(R9) (Rio), or -A2-heteroaryl wh _ein alkyl is straight or branched chain of 1 to 4 carb -,s;
Figure imgf000099_0001
represents a fused bicyclic ring v 'em
the monocyclic ring ^— is defined previously .ci
Figure imgf000099_0002
represents a substituted phenyl having two :oon
atoms in common with the monocyclic ring Vs— ; '.:
Figure imgf000099_0003
represents a spiro ring wherein
monocyclic ring
Figure imgf000099_0004
represents a heterocyclo ring having a common carb with
-N~ the monocyclic ring Vs—
3. A compound of Claim 2 including a pharmaceutically acceptable salt thereof wherein: Z is CH;
R3 is ethyl; 0/15222
Ei is or -0-CH2- disubstituted
Figure imgf000100_0001
phenyl;
E2 is -NH-CH2-disubstituted phenyl; the term "disubstituted phenyl" refers to a phenyl ring having two substituents independently selected from halogen and methoxy or wherein said disubstituted phenyl
Figure imgf000100_0002
X: is -0-Aχ-heterocyclo, -O-Ai-heteroaryl, -NH-A2-R2,
Figure imgf000100_0003
X2 i s -O-Ai-heterocyclo , -O-Ai-heteroaryl , -NH-A2-R25 ,
Figure imgf000101_0001
4. A compound of Claim 3 including a pharmaceutically acceptable salt thereof wherein:
Figure imgf000101_0002
Xi and X2 are independently selected from the group consistinσ of
Figure imgf000101_0003
Figure imgf000102_0001
O C02C(CH3)3
-HN— (CH2)2-N NH2
N ' "CH H 2- χ
Figure imgf000102_0002
-0-(CH2)2-N O -NH— (CH2)2- N
Figure imgf000102_0003
Figure imgf000102_0004
— NH-(CH2)3— -C02CH3
5. The compound of Claim 4 including a pharmaceutically acceptable salt thereof wherein:
Figure imgf000103_0001
6. A compound of Claim 4 including a pharmaceutically acceptable salt thereof wherein:
Figure imgf000103_0002
X? i s
Figure imgf000103_0003
Figure imgf000104_0001
H2
-o— c- -0-(CH2)2-N -NH-(CH2)2- W X N
Figure imgf000104_0002
-NH-(CH2)3 — C02CH3
7. The compound of Claim 6 including a pharmaceutically acceptable salt thereof wherein:
Figure imgf000104_0003
8. A compound of Claim 1 selected from the group consisting of:
4- [ t [4- f [ (3-chloro-4-methoxyphenyl) methyl] amino] -1- ethyl-lH-pyrazolo [3, 4-b] pyridin-5-yl] carbonyl] amino] -1- piperidinecarboxylic acid, ethyl ester; 4- [ [ (3-chloro-4-methoxyphenyl)methyl] amino] -1-ethyl- N- [3- (2-oxo-l-pyrrolidinyl) propyl] -IH-pyrazolo [3,4- b] pyridine-5-carboxamide;
4- [ [ (3-chloro-4-methoxyphenyl) methyl] amino] -1-ethyl- N- (2-pyridinylmethyl) -IH-pyrazolo [3, 4-b] pyridine-5- carboxamide;
4- [ [ (3-chloro-4-methoxyphenyl)methyl] amino] -1-ethyl- N- (3-pyridinylmethyl) -IH-pyrazolo [3, 4-b] pyridine-5- carooxamide;
4- [ [ ; 3-chioro-4-methoxyphenyl) methyl j amino] -1-etnyl- - (4-pyridinylmethyl) -IH-pyrazolo [3, -bj pyridine-5- carboxamide;
4- [ [ ( 3-chloro-4-methoxyphenyl)methyl] amino] -1-ethyl- N- [ ( -hydroxy-3-methoxyphenyl) ethyl] -IH-pyrazolo [3,4- b] pyridine-5-carboxamide;
4- [ [ ( 3-chloro-4-methoxyphenyl) methyl j amino] -1-ethyl- N- [ [4- (1, 2, 3-thiadiazol-4-yl)phenyl]methyij -IH- pyrazolo [3, 4-b] pyridine-5-carboxamide;
N- [ [4- [ [ (3-chloro-4-methoxyphenyl) methyl] amino] -1- ethyl-IH-pyrazolo [3, 4-b] pyridine-5-yl] carbonyl] glycine, ethyl ester;
4- [ [ (3-chloro-4-methoxyphenyl)methyl] amino] -1-ethyl- N- (3-lH-imidazol-l-ylpropyl) -IH-pyrazolo [3, 4-b] pyridine- 5-carboxamide; N- [ (3-chloro-4-methoxyphenyl) methyl] -4- [ [ (3-chloro- 4-methoxyphenyl) methyl] amino] -1-ethyl-IH-pyrazolo [3,4- b] pyridine-5-carboxamide;
(R) -α- [ [4- [ [ ( 3-chloro-4-methoxyphenyl) methyl] amino] - 1-ethyl-IH-pyrazolo [3, -b] pyridin-5-yl] carbonyl] - amino] benzeneacetic acid, ethyl ester;
4- [ [ (3-chloro-4 -methoxyphenyl) methyl] amino] -1-ethyl- N- [1- (phenylmethyl) -4-piperidinyl] -IH-pyrazolo [3, 4- b] pyridine-5-carboxamide;
4- [ [ (3-chloro-4 -me hoxyphenyl) ethyl] a ino] -i-ethyl- N- [3- (4-morpholinyl) propyl] -IH-pyrazolo [3, 4-b] pyridine-5- carboxamide;
4- [ [ (3-chloro-4-metnoxyphenyi)me hyi j amino] -1-ethyl- N- [ (4-pyridinyl-l-oxide) methyl] -IH-pyrazolo [3,4- b] pyridine-5-carboxamide;
4- [ [ (3-chloro-4-methoxyphenyl) methyl] amino] -1-ethyl- N- (2-furanylmethyl ) -IH-pyrazolo [3, 4-b] pyridine-5- carboxamide;
N- [ [4- [ [ (3-chloro-4-methoxyphenyl) methyl] amino] -1- ethyl-IH-pyrazolo [3, 4-b] pyridin-5-yl] carbonyl] -L-valine, ethyl ester;
4- [ [ (3-chloro-4-methoxyphenyl) methyl] amino] -1-ethyl- N- (1,2, 3, 4-tetrahydro-l-naphthylenyl) -IH-pyrazolo [3, 4- b] pyridine-5-carboxamide;
4- [ [ (3-chloro- -methoxyphenyl) methyl] amino] -1-ethyl- N- (tetrahydro-2-furanylmethyl) -IH-pyrazolo [3, 4- b]pyridine-5-carboxamide; 4- [ [ (3-chloro-4-methoxyphenyl)methyl] amino] -N- [ [4- (dimethylamino) phenyl]methyl] -1-ethyl-lH-pyrazolo [3, 4- b]pyridine-5-carboxamide;
N- [ [4- [ [ (3-chloro-4-methoxyphenyl)methyl] amino] -1- ethyl-lH-pyrazolo [3, 4-b] pyridin-5-yl] carbonyl] -L-alanine, methyl ester;
4- [ [ (3-chloro- -methoxyphenyl) methyl] amino] -1-ethyl- N- [ [4-fluoro-2- ( trifluoromethyl) phenyl] methyl] -IH- pyrazolo [3, -b] pyridine-5-carboxamide;
N- [ [4- [ [ (3-chloro-4 -methoxyphenyl) methyl] amino] -1- ethyl-lH-pyrazolo [3, 4-b] pyridin-5-yl] carbonyl] -L- methionine, methyl ester;
2- [ [ [4- [ [ ( 3-chloro-4-methoxyphenyl) methyl] amino] -1- ethyl-lH-pyrazolo [3, 4-b] pyridin-5-yl] carbonyl] - amino] propanedioic acid, diethyl ester;
4- [ [ (3-chloro- -methoxyphenyl) methyl] amino] -1-ethyl- N- (2-oxo-2-phenylethyl) -IH-pyrazolo [3, 4-b] pyridine-5- carboxamide;
4- [ [ (3-chloro-4-methoxyphenyl)methyl] amino] -1-ethyl- N- [2- (4-morpholinyl) ethyl] -IH-pyrazolo [3, 4-b] pyridine-5- carboxamide;
N- [ [4- [ [ (3-chloro-4-methoxyphenyl)methyl] amino] -1- ethyl-lH-pyrazolo [3, 4-b]pyridin-5-yl] carbonyl] -L- asparagine, 1, 1-dimethylethyl ester;
(S) -4- [ [ (3-chloro-4-methoxyphenyl) methyl] amino] -1- ethyl-N- [2-oxo-3- [2-oxo-2- (1-pyrrolidinyl) - ethyl] cycloheptyl] -IH-pyrazolo [3, -b] pyridine-5- carboxamide;
(S) -α- [ [ [4- [ [ (3-chloro-4-methoxyphenyl)methyl] - amino] -1-ethyl-IH-pyrazolo [3, 4-b] pyridine-5- yl] carbonyl] amino] -4-hydroxybenzeneacetic acid;
4- [ [ (3-chloro-4-methoxyphenyl) methyl] amino] -1-ethyl- N- (2-pyridinylmethyl) -IH-pyrazolo [3, -b] pyridin-5- carboxylate;
4- [ [ (3-chloro-4-methoxyphenyl ) methyl] amino] -1-ethyl- N- (4-pyridinylmethyl} -IH-pyrazolo [ 3 , 4-b] pyri in-5- carboxyiate;
4- [ [ (3-chloro-4-methoxyphenyl) methyl] amino] -1-ethyl- N- [3- i -morpholinyl) ethyl] -IH-pyrazolo [3, 4-b] pyridin-5- carboxylate;
4- [ [ (3-chloro- -methoxyphenyl) methyl] amino] -1-ethyl- N- (4-pyridinylethyi] -IH-pyrazolo [3, -b] pyridin-5- carboxa ide;
4- [ [ (3-chloro- -methoxyphenyl) methyl] amino] -1-ethyl- N- (2-pyridinylethyl) -IH-pyrazolo [3, 4-b] pyridin-5- carboxamide;
(R) -4- [ [ (3-chloro-4-methoxyphenyl) ethyl] amino] -1- ethyl-N- (2-tetrahydrofurylmethyl) -IH-pyrazolo [3, 4- b]pyridine-5-carboxamide;
(S) -4- [ [ (3-chloro-4-methoxyphenyl) methyl] amino] -1- ethyl-N- (2-tetrahydrofurylmethyl) -IH-pyrazolo [3,4- b]pyridine-5-carboxamide; 4- [ [ (3-chloro-4-methoxyphenyl)methyl] amino] -1-ethyl- N- (hexahydro-2-oxo-lH-azepin-3-yl) -IH-pyrazolo [3,4- b] pyridin-5-carboxamide ;
4- [ [ (3-chloro-4-methoxyphenyl) methyl] amino] -N- (cyanomethyl) -1-ethyl-lH-pyrazolo [3, 4-b] pyridin-5- carboxamide;
(S) -α- [ [ [4- [ [ (3-chloro-4-methoxyphenyl) - methyl] amino] -1-ethyl-lH-pyrazolo [3, 4-b] pyridine-5- yl] carbonyl] amino-4-hydroxybenzeneacetic acid;
4- [ [ [4- [ [ (3-chloro-4-methoxyphenyl) ethyl] amino] -1- ethyl-lH-pyrazoio [3, 4-b] pyridine-5- yl] carbonyl] amino] butanoic acid, methyl ester; and;
4- [ [ (3-chloro-4-methoxyphenyl) methyl] hydroxyl] -1- ethyl-N- (4-pyridinylmethyl) -IH-pyrazolo [3, 4-b] pyridin-5- carboxamide or a pharmaceutically acceptable salt of any of the above compounds.
9. A pharmaceutical composition useful for treating a cGMP associated condition comprising a compound of Claim 1 including a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier.
10. A pharmaceutical composition useful for treating a cGMP associated condition comprising a compound of Claim 8 including a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier.
11. The pharmaceutical composition of Claim 10 wherein the compound is 4- [ [ (3-chloro-4- methoxyphenyl) methyl] amino] -1-ethyl-N- (4- pyridinylmethyl) -IH-pyrazolo [3, 4-b] pyridine-5-carboxamide or a pharmaceutically acceptable salt thereof.
12. A pharmaceutical composition useful for treating a cGMP associated condition comprising a pharmaceutically acceptable carrier and a compound of the formula
Figure imgf000110_0001
including a pharmaceutically acceptable salt thereof wherein E2, Y, Z, R3 and R4 are as defined in Claim 1;
X3 is -O-Rg, -O-Aι-0-Rg, -N(R9)(Rιo)
Figure imgf000110_0002
' and
Figure imgf000110_0003
Figure imgf000110_0004
Claim 1.
13. The composition of Claim 12 wherein:
Y is nitrogen;
Z is CH;
R3 is ethyl;
R4 is hydrogen;
Figure imgf000111_0001
—NH-CH—CH2—OCH3
X3 is
C2H5
14. A method of treating a mammalian host to relieve a cGMP associated condition comprising administering to said host an effective amount of a composition of Claim 9.
15. The method of Claim 14 wherein the cGMP associated condition being treated is erectile dysfunction.
16. A method of treating a mammalian host to relieve a cGMP associated condition comprising administering to said host an effective amount of a composition of Claim 10.
17. The method of Claim 16 wherein the cGMP associated condition being treated is erectile dysfunction.
18. A method of treating a mammalian host to relieve a cGMP associated condition comprising administering to said host an effective amount of a composition of Claim 12.
19. A method of Claim 18 wherein the cGMP associated condition being treated is erectile dysfunction.
PCT/US1999/021070 1998-09-16 1999-09-13 FUSED PYRIDINE INHIBITORS OF cGMP PHOSPHODIESTERASE WO2000015222A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU61438/99A AU751486B2 (en) 1998-09-16 1999-09-13 Fused pyridine inhibitors of cGMP phosphodiesterase
CA002342583A CA2342583A1 (en) 1998-09-16 1999-09-13 Fused pyridine inhibitors of cgmp phosphodiesterase
EP99948211A EP1113796A4 (en) 1998-09-16 1999-09-13 FUSED PYRIDINE INHIBITORS OF cGMP PHOSPHODIESTERASE
JP2000569806A JP2002524512A (en) 1998-09-16 1999-09-13 Condensed pyridine inhibitors of cGMP phosphodiesterase

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Cited By (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6187779B1 (en) 1998-11-20 2001-02-13 Cell Pathways, Inc. Method for inhibiting neoplastic cells and related conditions by exposure to 2,8-disubstituted quinazoline derivatives
US6211177B1 (en) 1998-11-24 2001-04-03 Cell Pathways, Inc. Method for treating neoplasia by exposure to substituted 2-aryl-benzimidazole derivatives
US6211220B1 (en) 1998-11-23 2001-04-03 Cell Pathways, Inc. Method for treating neoplasia with amino or pyridylamino cyclobutene derivatives
US6316438B1 (en) 1999-03-22 2001-11-13 Bristol-Myers Squibb Co. Fused pyridopyridazine inhibitors of cGMP phosphodiesterase
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US6348032B1 (en) 1998-11-23 2002-02-19 Cell Pathways, Inc. Method of inhibiting neoplastic cells with benzimidazole derivatives
US6358992B1 (en) 1998-11-25 2002-03-19 Cell Pathways, Inc. Method of inhibiting neoplastic cells with indole derivatives
US6369092B1 (en) 1998-11-23 2002-04-09 Cell Pathways, Inc. Method for treating neoplasia by exposure to substituted benzimidazole derivatives
US6410584B1 (en) 1998-01-14 2002-06-25 Cell Pathways, Inc. Method for inhibiting neoplastic cells with indole derivatives
US6486155B1 (en) 1998-11-24 2002-11-26 Cell Pathways Inc Method of inhibiting neoplastic cells with isoquinoline derivatives
WO2004024728A2 (en) * 2002-09-16 2004-03-25 Glaxo Group Limited Pyrazolo[3,4-b]pyridine compounds, and their use as phosphodiesterase inhibitors
JP2004518719A (en) * 2001-02-13 2004-06-24 アベンティス・ファーマ・ドイチユラント・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Acylated indanylamine and its use as a medicament
JP2004520268A (en) * 2000-08-22 2004-07-08 ベーリンガー インゲルハイム ファルマ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト Pharmaceutical composition containing an angiotensin II antagonist and an angiotensin I converting enzyme inhibitor
WO2004056823A1 (en) * 2002-12-23 2004-07-08 Glaxo Group Limited PYRAZOLO[3,4-b]PYRIDINE COMPOUNDS, AND THEIR USE AS PHOSPHODIESTERASE INHIBITORS
US6777421B2 (en) 2001-04-10 2004-08-17 Ortho-Mcneil Pharmaceutical, Inc. 1,3,8-Triazaspiro[4.5]decan-4-one derivatives useful for the treatment of ORL-1 receptor mediated disorders
FR2851247A1 (en) * 2003-02-19 2004-08-20 Exonhit Therapeutics Sa Use of a phosphodiesterase type 4 (PDE4) inhibitor particularly a pyrazolopyridine or a nucleic acid that inhibits the transcription of the PDE4B gene for the treatment of ocular degenerative disorders
WO2005021537A1 (en) 2003-08-21 2005-03-10 Osi Pharmaceuticals, Inc. N-substituted pyrazolyl-amidyl-benzimidazolyl c-kit inhibitors
WO2005021544A2 (en) 2003-08-21 2005-03-10 Osi Pharmaceuticals, Inc. N3-substituted imidazopyridine-derivatives as c-kit inhibitors
WO2005058892A1 (en) * 2003-12-19 2005-06-30 Glaxo Group Limited Pyrazolo [3,4-b] pyridine compounds, and their use as phosphodiesterase inhibitors
WO2006004188A1 (en) * 2004-07-05 2006-01-12 Astellas Pharma Inc. Pyrazolopyridine derivatives
US7442709B2 (en) 2003-08-21 2008-10-28 Osi Pharmaceuticals, Inc. N3-substituted imidazopyridine c-Kit inhibitors
US7709497B2 (en) 2004-03-16 2010-05-04 Glaxo Group Limited Pyrazolo[3,4-b]pyridine compound, and its use as a PDE4 inhibitor
US7915286B2 (en) 2005-09-16 2011-03-29 Ranbaxy Laboratories Limited Substituted pyrazolo [3,4-b] pyridines as phosphodiesterase inhibitors
US8034811B2 (en) 2003-12-03 2011-10-11 Leo Pharma A/S Hydroxamic acid esters and pharmaceutical use thereof
US8067408B2 (en) 2008-02-06 2011-11-29 Glaxo Group Limited Dual pharmacophores—PDE4-muscarinic antagonistics
US8071588B2 (en) 2008-02-06 2011-12-06 Glaxo Group Limited Dual pharmacophores—PDE4-muscarinic antagonistics
US8084449B2 (en) 2008-02-06 2011-12-27 Glaxo Group Limited Dual pharmacophores—PDE4-muscarinic antagonistics
US8642624B2 (en) 2001-01-12 2014-02-04 Amgen Inc. Substituted alkylamine derivatives and methods of use
US8697875B2 (en) 2008-12-23 2014-04-15 The Trustees Of Columbia University In The City Of New York Phosphodiesterase inhibitors and uses thereof
US8703948B2 (en) 2006-11-28 2014-04-22 Janssen Pharmaceutica Nv Salts of 3-(3-amino-2-(R)-hydroxy-propyl)-1-(4-fluoro-phenyl)-8-(8-methyl-naphthalen-1-ylmethyl)-1,3,8-triaza-spiro[4.5]decan-4-one
US8741916B2 (en) 2007-04-09 2014-06-03 Janssen Pharmaceutica Nv 1,3,8-trisubstituted-1,3,8-triaza-spiro[4.5]decan-4-one derivatives as ligands of the ORL-1 receptor
US8778956B2 (en) 2002-09-09 2014-07-15 Janssen Pharmaceutica Nv Hydroxy alkyl substituted 1,3,8-triazaspiro[4.5]decan-4-one derivatives useful for the treatment of ORL-1 receptor mediated disorders
CN108395377A (en) * 2018-01-16 2018-08-14 吴江信凯医药科技有限公司 A kind of preparation method of 3- chloro-4-methoxies benzylamine hydrochloride
US11491140B2 (en) 2016-07-04 2022-11-08 H. Lundbeck A/S 1H-pyrazolo[4,3-b]pyridines as PDE1 inhibitors
US11535611B2 (en) 2017-12-20 2022-12-27 H. Lundbeck A/S Pyrazolo[3,4-B]pyridines and imidazo[1,5-B]pyridazines as PDE1 inhibitors
US11634416B2 (en) 2017-12-14 2023-04-25 H. Lundbeck A/S Combination treatments comprising administration of 1H-pyrazolo[4,3-b]pyridines
US11851425B2 (en) 2017-12-14 2023-12-26 H. Lundbeck A/S Combination treatments comprising administration of 1H-pyrazolo[4,3-B]pyridines

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6962926B2 (en) * 2001-01-31 2005-11-08 Telik, Inc. Antagonist of MCP-1 function, and compositions and methods of use thereof
US6670364B2 (en) * 2001-01-31 2003-12-30 Telik, Inc. Antagonists of MCP-1 function and methods of use thereof
CZ20032990A3 (en) * 2001-05-05 2004-04-14 Smithkline Beecham P. L. C. N-aroyl cyclic amines
US20040192673A1 (en) * 2001-05-05 2004-09-30 Pascale Gaillard N-aroyl cyclic amine derivatives as orexin receptor antagonists
EP1705179A1 (en) * 2001-06-28 2006-09-27 Smithkline Beecham Plc N-aroyl cyclic amine derivatives as orexin receptor antagonists
GB0115862D0 (en) * 2001-06-28 2001-08-22 Smithkline Beecham Plc Compounds
EP1388535A1 (en) 2002-08-07 2004-02-11 Aventis Pharma Deutschland GmbH Acylated arylcycloalkylamines and their use as pharmaceuticals
PT1644021E (en) * 2003-06-13 2013-01-30 Ironwood Pharmaceuticals Inc Methods and compositions for the treatment of gastrointestinal disorders
UA82395C2 (en) * 2003-08-21 2008-04-10 Оси Фармасьютикалз, Инк. N-substituted benzimidazolyl c-kit inhibitors
GB0405937D0 (en) * 2004-03-16 2004-04-21 Glaxo Group Ltd Compounds
KR20070086865A (en) * 2004-12-01 2007-08-27 오에스아이 파마슈티컬스, 인코포레이티드 N-substituted benzimidazolyl c-kit inhibitors and combinatorial benzimidazole library
EP3317279B1 (en) * 2015-07-03 2021-01-27 H. Hoffnabb-La Roche Ag Triaza-spirodecanones as ddr1 inhibitors
WO2017168174A1 (en) 2016-04-02 2017-10-05 N4 Pharma Uk Limited New pharmaceutical forms of sildenafil
WO2018002673A1 (en) 2016-07-01 2018-01-04 N4 Pharma Uk Limited Novel formulations of angiotensin ii receptor antagonists
JOP20190126A1 (en) * 2016-12-22 2019-05-28 H Lundbeck As Pyrazolo[3,4-b]pyridines and imidazo[1,5-b]pyridazines as pde1 inhibitors

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3855675A (en) 1971-05-25 1974-12-24 Squibb & Sons Inc 1-(2-furanylmethyl)-1h-pyrazolo(3,4-b)pyridine-5-methanones
BE787249A (en) 1971-08-05 1973-02-05 Squibb & Sons Inc AMINO DERIVATIVES OF PYRAZOLOPYRIDINE CARBOXYLIC ACIDS, THEIR ESTERS AND THE SALTS OF SUCH COMPOUNDS, AS WELL AS THEIR PREPARATION PROCESSES
US3787430A (en) 1972-07-13 1974-01-22 Squibb & Sons Inc Derivatives of dipyrazolo(3,4-b;3',4'-d)pyridines
US3840546A (en) 1972-11-15 1974-10-08 Squibb & Sons Inc Amino derivatives of pyrazolopyridine carboxamides
US3979399A (en) 1972-11-15 1976-09-07 E. R. Squibb & Sons, Inc. Amino derivatives of pyrazolopyridine carboxamides
US4051236A (en) 1973-02-15 1977-09-27 E. R. Squibb & Sons, Inc. Inhibition of blood platelet aggregation
US4070362A (en) 1974-02-21 1978-01-24 E. R. Squibb & Sons, Inc. Amino derivatives of triazolo(4,5-B)pyridines
GB1460059A (en) 1974-05-06 1976-12-31 Squibb & Sons Inc Derivatives of pyrazolopyridine
US4003903A (en) 1975-02-12 1977-01-18 Florida Board Of Regents N-acyl-N-norsalutaridines and process for making them
US3987051A (en) 1975-04-16 1976-10-19 E. R. Squibb & Sons, Inc. Method for producing 1-unsubstituted pyrazolo[3,4-b]pyridine ketones
US4003908A (en) 1976-03-11 1977-01-18 E. R. Squibb & Sons, Inc. Derivatives of imidazo(4,5-b)pyridines
GB8425104D0 (en) 1984-10-04 1984-11-07 Ici America Inc Amide derivatives
US5250534A (en) 1990-06-20 1993-10-05 Pfizer Inc. Pyrazolopyrimidinone antianginal agents
GB9114760D0 (en) 1991-07-09 1991-08-28 Pfizer Ltd Therapeutic agents
GB9121028D0 (en) * 1991-10-03 1991-11-13 Pfizer Ltd Therapeutic agents
GB9126260D0 (en) 1991-12-11 1992-02-12 Pfizer Ltd Therapeutic agents
GB9213623D0 (en) * 1992-06-26 1992-08-12 Pfizer Ltd Therapeutic agents
GB9315017D0 (en) * 1993-07-20 1993-09-01 Glaxo Lab Sa Chemical compounds
TW334423B (en) 1993-10-22 1998-06-21 Hoffmann La Roche Tricyclic 1-aminoethylpyrrole-derivatives
TW270114B (en) 1993-10-22 1996-02-11 Hoffmann La Roche
GB9401090D0 (en) 1994-01-21 1994-03-16 Glaxo Lab Sa Chemical compounds
GB9514465D0 (en) 1995-07-14 1995-09-13 Glaxo Lab Sa Chemical compounds
US5612359A (en) 1994-08-26 1997-03-18 Bristol-Myers Squibb Company Substituted biphenyl isoxazole sulfonamides
US5614530A (en) * 1995-03-10 1997-03-25 Sterling Winthrop Inc. Substituted N-arylmethyl and heterocyclmethyl-1H-pyrazolo[3,4-b]quinolin-4-amines and compositions and methods of use thereof
US5488055A (en) 1995-03-10 1996-01-30 Sanofi Winthrop Inc. Substituted N-cycloalkylmethyl-1H-pyrazolo(3,4-b)quinolin-4 amines and compositions and methods of use thereof
GB9514464D0 (en) 1995-07-14 1995-09-13 Glaxo Lab Sa Medicaments
ZA969888B (en) 1995-11-28 1997-05-26 Schering Corp 2'-[[4'-halo-[1,1-biphenyl]-4-yl]methyl]-5'-methyl-spiro[cyclopentane-1,7'(8'H)-[3H]imidazo[2,1-b]purin]-4'(5'H)-ones
WO1997023480A1 (en) 1995-12-22 1997-07-03 The Du Pont Merck Pharmaceutical Company Novel integrin receptor antagonists
DE19625263A1 (en) 1996-06-25 1998-01-02 Bayer Ag Substituted N- (4-pyridyl) thioamides
CN1121220C (en) 1996-08-20 2003-09-17 卫材株式会社 Remedy for erection failure comprising fused pyridazine compound
WO1998008848A1 (en) 1996-08-30 1998-03-05 Kyowa Hakko Kogyo Co., Ltd. Imidazoquinazoline derivatives
EP1043998B1 (en) * 1997-12-13 2007-03-07 Bristol-Myers Squibb Company USE OF PYRAZOLO [3,4-b] PYRIDINE AS CYCLIN DEPENDANT KINASE INHIBITORS

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
BIOCHEM. PHARMACOL., vol. 38, no. 19, 1989, pages 3331 - 3339 *
DATABASE CA ON STN CHEMICAL ABSTRACTS (COLUMBUS, OHIO, USA); AHN H.S.: "Effects of selective inhibitors on cyclic nucleotide phosphodiesterases of rabbit aorta" *
DATABASE CA ON STN CHEMICAL ABSTRACTS (COLUMBUS, OHIO, USA); DAVIS A.: "Strategic approaches to drug design" *
DATABASE CA ON STN CHEMICAL ABSTRACTS (COLUMBUS, OHIO, USA); KURTZ M.K.: "Cyclic nucleotide phosphodiesterases in cultured normal and RCS rat pigment epithelium: kinetics of cyclic AMP and cyclic GMP hydrolysis" *
DATABASE CA ON STN CHEMICAL ABSTRACTS (COLUMBUS, OHIO, USA); OQUIST N.L.: "Inotropic responses to selective (RO 20-1724 and SQ 65,442) and nonselective (trequinsin) inhibitors of cyclic AMP-specific class IV phosphodiesterase in newborn, immature and adult rabbit myocardium" *
DATABASE CA ON STN CHEMICAL ABSTRACTS (COLUMBUS, OHIO, USA); TOMES C.: "cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase" *
EXP. EYE RES., vol. 45, no. 1, 1987, pages 67 - 75 *
INT. J. BIOCHEM., vol. 22, no. 9, 1990, pages 1047 - 1051 *
J. COMPUT.-AIDED MOL. DES., vol. 1, no. 2, 1987, pages 97 - 119 *
PEDIATR. RES., 1992 *
See also references of EP1113796A4 *

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US6410584B1 (en) 1998-01-14 2002-06-25 Cell Pathways, Inc. Method for inhibiting neoplastic cells with indole derivatives
US6187779B1 (en) 1998-11-20 2001-02-13 Cell Pathways, Inc. Method for inhibiting neoplastic cells and related conditions by exposure to 2,8-disubstituted quinazoline derivatives
US6348032B1 (en) 1998-11-23 2002-02-19 Cell Pathways, Inc. Method of inhibiting neoplastic cells with benzimidazole derivatives
US6211220B1 (en) 1998-11-23 2001-04-03 Cell Pathways, Inc. Method for treating neoplasia with amino or pyridylamino cyclobutene derivatives
US6369092B1 (en) 1998-11-23 2002-04-09 Cell Pathways, Inc. Method for treating neoplasia by exposure to substituted benzimidazole derivatives
US6486155B1 (en) 1998-11-24 2002-11-26 Cell Pathways Inc Method of inhibiting neoplastic cells with isoquinoline derivatives
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US6316438B1 (en) 1999-03-22 2001-11-13 Bristol-Myers Squibb Co. Fused pyridopyridazine inhibitors of cGMP phosphodiesterase
JP2004520268A (en) * 2000-08-22 2004-07-08 ベーリンガー インゲルハイム ファルマ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト Pharmaceutical composition containing an angiotensin II antagonist and an angiotensin I converting enzyme inhibitor
US8642624B2 (en) 2001-01-12 2014-02-04 Amgen Inc. Substituted alkylamine derivatives and methods of use
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US8163751B2 (en) 2001-02-13 2012-04-24 Sanofi-Aventis Deutschland Gmbh Acylated indanyl amines and their use as pharmaceuticals
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US7053101B2 (en) 2001-04-10 2006-05-30 Alfonzo Jordan 1,3,8-triazaspiro[4,5]decan-4-one derivatives useful for the treatment of ORL-1 receptor mediated disorders
US6777421B2 (en) 2001-04-10 2004-08-17 Ortho-Mcneil Pharmaceutical, Inc. 1,3,8-Triazaspiro[4.5]decan-4-one derivatives useful for the treatment of ORL-1 receptor mediated disorders
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AU2004212736B2 (en) * 2003-02-19 2009-04-30 Exonhit Therapeutics S.A. Methods involving PDE4, compositions, and the screening thereof, for the treatment of degenerative ocular pathologies
WO2004073711A3 (en) * 2003-02-19 2005-04-14 Exonhit Therapeutics Sa Methods involving pde4, compositions, and the screening thereof, for the treatment of degenerative ocular pathologies
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US7442709B2 (en) 2003-08-21 2008-10-28 Osi Pharmaceuticals, Inc. N3-substituted imidazopyridine c-Kit inhibitors
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US7767673B2 (en) 2003-08-21 2010-08-03 Osi Pharmaceuticals, Inc. N-substituted imidazopyridine c-Kit inhibitors
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US8034811B2 (en) 2003-12-03 2011-10-11 Leo Pharma A/S Hydroxamic acid esters and pharmaceutical use thereof
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WO2006004188A1 (en) * 2004-07-05 2006-01-12 Astellas Pharma Inc. Pyrazolopyridine derivatives
US7915286B2 (en) 2005-09-16 2011-03-29 Ranbaxy Laboratories Limited Substituted pyrazolo [3,4-b] pyridines as phosphodiesterase inhibitors
US8703948B2 (en) 2006-11-28 2014-04-22 Janssen Pharmaceutica Nv Salts of 3-(3-amino-2-(R)-hydroxy-propyl)-1-(4-fluoro-phenyl)-8-(8-methyl-naphthalen-1-ylmethyl)-1,3,8-triaza-spiro[4.5]decan-4-one
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AR021811A1 (en) 2002-08-07
AU751486B2 (en) 2002-08-15
US6326379B1 (en) 2001-12-04
EP1113796A4 (en) 2003-07-30

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