WO2000004152A2 - Androgen receptor coactivators - Google Patents
Androgen receptor coactivators Download PDFInfo
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- WO2000004152A2 WO2000004152A2 PCT/US1999/016122 US9916122W WO0004152A2 WO 2000004152 A2 WO2000004152 A2 WO 2000004152A2 US 9916122 W US9916122 W US 9916122W WO 0004152 A2 WO0004152 A2 WO 0004152A2
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
- G01N2333/723—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
Definitions
- Androgens constitute a class of hormones that control the development and proper function of mammalian male reproductive systems, including the prostate and epididymis. Androgens also affect the physiology of many non-reproductive systems, including muscle, skin, pituitary, lymphocytes, hair growth, and brain. Androgens exert their effect by altering the level of gene expression of specific genes in a process that is mediated by binding of androgen to an androgen receptor.
- the androgen receptor which is a member of the steroid receptor super family, plays an important role in male sexual differentiation and in prostate cell proliferation. Binding of androgen by the androgen receptor allows the androgen receptor to interact with androgen responsive element (AREs) , DNA sequences found on genes whose expression is regulated by androgen.
- AREs androgen responsive element
- Androgen-mediated regulation of gene expression is a complicated process that may involve multiple co-activators (Adler et al . , Proc. National Acad. Sci . USA 89:6319-6325, 1992) .
- a fundamental question in the field of steroid hormone biology is how specific androgen-activated transcription can be achieved in vivo when several different receptors recognize the same DNA sequence.
- the androgen receptor (AR) , the glucocorticoid receptor (GR) , and the progesterone receptor (PR) all recognize the same sequence but activate different transcription activities.
- Prostate cancer is the most common malignant neoplasm in aging males in the United States. Standard treatment includes the surgical or chemical castration of the patient in combination with the administration of anti-androgens such as 17 ⁇ estradiol (E2) or hydroxyflutamide (HF) .
- anti-androgens such as 17 ⁇ estradiol (E2) or hydroxyflutamide (HF) .
- E2 17 ⁇ estradiol
- HF hydroxyflutamide
- Prostate cancers treated with androgen ablation and anti-androgens progress from an androgen- dependant to an androgen- independent state, causing a high incidence of relapse within 18 months (Crawford, Br . J. Urology 70: suppl . 1, 1992) .
- the mechanisms by which prostate cancer cells become resistant to hormonal therapy remain unclear.
- A1B1 was identified as estrogen receptor coactivator that is expressed at higher levels in ovarian cancer cell lines and breast cancer cells than in noncancerous cells (Anzick, et al . Science 277:965-968,
- the present invention includes an isolated polynucleotide that encodes a co-activator for human androgen receptor, the polynucleotide comprising a sequence that encodes a polypeptide selected from the group consisting of an ARA54 polypeptide, an ARA55 polypeptide, an ARA24 polypeptide, and an Rb polypeptide.
- Another aspect of the present invention is a genetic construct comprising a promoter functional in a prokaryotic or eukaryotic cell operably connected to a polynucleotide that encodes a polypeptide selected from the group consisting of an ARA54 polypeptide, an ARA55 polypeptide, an ARA24 polypeptide and an Rb polypeptide.
- the present invention provides a method for screening candidate pharmaceutical molecules for the ability to promote or inhibit the interaction of ARs and AREs to modulate androgenic activity comprising the steps of: (a) providing a genetic construct comprising a promoter functional in a eukaryotic cell operably connected to a polynucleotide comprising a sequence that encodes a polypeptide selected from the group consisting of an ARA54 polypeptide, an ARA55 polypeptide, an ARA24 polypeptide, and a retinoblastoma polypeptide; (b) cotransforming a suitable eukaryotic cell with the construct of step a, and a construct comprising at least a portion of an expressible androgen receptor sequence;
- One aspect of the present invention is an isolated polynucleotide that encodes a co-activator for human androgen receptor, the polynucleotide comprising a sequence that encodes a polypeptide selected from the group consisting of an ARA54 polypeptide, an ARA55 polypeptide, an ARA24 polypeptide and an Rb polypeptide.
- Another aspect of the present invention is a genetic construct comprising a promoter functional in a prokaryotic or eukaryotic cell operably connected to a polynucleotide that encodes a polypeptide selected from the group consisting of an ARA54 polypeptide, an ARA55 polypeptide, an ARA24 polypeptide and an Rb polypeptide.
- the present invention includes a method for screening candidate pharmaceutical molecules for the ability to promote or inhibit the ARs and AREs to result in modulation of androgenic effect comprising the steps of:
- a genetic construct comprising a promoter functional in a eukaryotic cell operably connected to a polynucleotide comprising a sequence that encodes a polypeptide selected from the group consisting of an ARA54 polypeptide, an ARA55 polypeptide, an ARA24 polypeptide, and a retinoblastoma polypeptide;
- a suitable eukaryotic cell with the construct of step a, and a construct comprising at least a portion of an expressible androgen receptor sequence;
- the human androgen receptor is comprised of a ligand binding domain (LBD) , a DNA binding domain (DBD) , a hinge domain containing nuclear localization signals, and a transactivation domain in the hyper-variable N-terminus. Truncation or deletion of the LBD results in constitutive transactivation by the N- terminal domain.
- progression of prostate cancer from androgen dependent- to androgen independent -stage may be caused by a mutation in the LBD that alters the ligand specificity of the mAR (Taplan et al . , New Engl . J. Med . 332:1393-1398 (1995); Gaddipati et al . , Cancer Res . 54:2861-2864 (1994)) .
- mAR ligand specificity of wild type AR and mAR involves the use of different androgen receptor-associated (ARA) proteins or coactivators by these receptors.
- a yeast two-hybrid system with mART887S as bait was used to screen the human prostate cDNA library.
- the sequences of two clones encoding a putative coactivators are shown in SEQ ID NO : 1 and SEQ ID NO : 3 , respectively.
- the putative amino acid sequences of ARA54 and ARA55 are shown in SEQ ID NO : 2 and SEQ ID NO : 4 , respectively.
- Also provided are the DNA and amino acid sequences of ARA24 (SEQ ID NO: 5 and SEQ ID NO : 6 , respectively) and Rb (SEQ ID NO : 7 and SEQ ID NO : 8 , respectively) .
- ARA54 is a 54 kDa protein that interacts with AR in an androgen-dependent manner. Coexpression of ARA54 and AR in a mammalian two-hybrid system demonstrated that reporter gene activity was enhanced in an androgen- dependent manner. ARA54 functions as a coactivator relatively specific for AR-mediated transcription. However, ARA54 may also function as a general coactivator of the transcriptional activity for other steroid receptors through their cognate ligands and response elements. ARA54 was found to enhance the transcriptional activity of AR and PR up to 6 fold and 3-5 fold, respectively. In contrast, ARA54 has only marginal effects (less than 2 fold) on glucocorticoid receptor (GR) and estrogen receptor (ER) in DU145 cells.
- GR glucocorticoid receptor
- ER estrogen receptor
- the C-terminal domain of ARA54 (a. a. 361-471 of SEQ ID NO:l) serves as a dominant negative inhibitor of AR- mediated gene expression of target genes. Coexpression of exogenous full-length ARA54 can reduce this squelching effect in a dose-dependent manner.
- ARA54 enhanced transactivation of wtAR in the presence of DHT (10- 10 to 10 '8 M) by about 3-5 fold. However, transactivation of wtAR was enhanced only marginally with E2 (10 "9 -10- 7 M) or HF (10- 7 -10- 5 M) as the ligand.
- E2 (10 "9 -10- 7 M)
- HF 10- 7 -10- 5 M
- the ability of ARA54 to enhance transactivation by two mutant receptors (mARt877a and mARe708k) that exhibit differential sensitivities to E2 and HF was also examined.
- mutant mARt877a which is found in many prostate tumors (23), was activated by E2 (10- 9 -10- 7 M) and HF (10 "7 -10- 5 M) , and ARA54 could further enhance E2- or HF-mediated AR transactivation.
- mutant mARe708k first identified in a yeast genetic screening (Wang, C.,Ph.D. Thesis of Universi ty of Wisconsin -Madison (1997) ) , exhibited ligand specificity and response to ARE54 comparable to that of wtAR.
- An ARA54 polypeptide is a polypeptide that is capable of enhancing transactivation of AR in an androgen-dependent manner, enhancing E2 or HF transactivation by the mutant receptor mARt877a, and reducing inhibition of AR-mediated gene expression caused by overexpression of the C-terminal domain of ARA54 (a. a. 361-471 of SEQ ID N0:1) .
- sequence information presented in this application can be used to identify, clone or sequence allelic variations in the ARA54 genes as well as the counterpart genes from other mammalian species, it is also contemplate that truncations of the native coding region can be made to express smaller polypeptides that will retain the same biological activity.
- ARA55 The polynucleotide sequence of ARA55 (SEQ ID NO : 3 ) exhibits high homology to the C-terminus of mouse hic5 (hydrogen peroxide inducible clone) (Pugh, B., Curr . Opin . Cell Biol . 8:303-311 (1996)), and like hic5, ARA55 expression is induced by TGFb .
- Cotransfection assays of transcriptional activation which are described in detail below, revealed that ARA55 is able to bind to both wtAR and mART887S in a ligand-dependent manner to enhance AR transcriptional activities.
- ARA55 enhanced transcriptional activation by wtAR in the presence of 10 ⁇ 9 M DHT or T, but not 10 ⁇ 9 M E2 or HF .
- ARA55 can enhance transcriptional activation by mART887S in the presence of DHT, testosterone (T) , E2 , or HF.
- ARA55 did not enhance transcriptional activation of mARe708k in the presence of E2 , but can enhance transcription in the presence of DHT or T.
- the C-terminal domain of ARA55 (amino acids 251-444 of SEQ ID NO: 3) is sufficient for binding to ARs , but does not enhance transcriptional activation by ARs.
- an ARA55 polypeptide it meant a polypeptide that is capable of enhancing transactivation of wtAR, , the mutant receptor mARt877a, in the presence of DHT, E2, or HF or intact receptor mARe708k in the presence of DHT or T.
- Such polypeptides include allelic variants and the corresponding genes from other mammalian species as well as truncations.
- the AR N-terminal domain comprises a polymorphic poly- glutamine (Q) stretch and a polymorphic poly-glycine (G) stretch that account for variability in the length of human AR cDNA observed.
- the length of the poly-Q region (normally 11-33 residues in length) is inversely correlated with the risk of prostate cancer, and directly correlated with the SBMA, or Kennedy's disease (La Spada et al . , Nature (London) 352:77-79 (1991)).
- the incidence of higher grade, distant metastatic, and fatal prostate cancer is higher in men having shorter AR poly-Q stretches.
- the ARA24 clone has an ORF that is identical to the published ORF for human Ran, an abundant, ras-like small GTPase (Beddow et al . Proc . Na tl . Acad . Sci . USA 92:3328- 3332, 1995) .
- Overexpression of ARA24 in the presence of DHT does enhance transcriptional activation by AR over that observed in cells transfected with AR alone.
- expression of antisense ARA24 (ARA24as) does reduce DHT- induced transcriptional activation.
- An ARA24 polypeptide is one that interacts with the poly-Q region of an AR.
- An ARA24 polypeptide is further characterized by its ability to increase transactivation when overexpressed in eukaryotic cells having some endogenous ARA24, but expression of an ARA24 antisense RNA reduces AR receptor transactivation.
- Androgen receptor mutations do not account for all cases of androgen-independent tumors, because some androgen- independent tumors retain wild-type AR.
- a significant percentage of androgen- insensitive tumors have been correlated with reduced expression of retinoblastoma protein (Rb) (Bookstein, et al . , Science 247 :712-715, (1990) ) , expression a truncated Rb protein (Bookstein, et al . Proc . Na tl . Acad . Sci . USA 87:7762-7766 (1990)), or a missing Rb allele (Brooks, et al . Prosta te 26:35-39, (1995) ) .
- Rb retinoblastoma protein
- the prostate cancer cell line DU145 has an abnormal short mRNA transcript of Rb exon 21 (Sarkar, et al . Prostate 21:145-152(1992)) and transfecton of the wild- type Rb gene into DU145 cells was shown to repress the malignant phenotype (Bookstein, et al . Proc . Natl . Acad . Sci . USA 87:7762-7766 (1990)).
- Rb functions in the control of cell proliferation and differentiation (Weinberg, R.A., Cell 81:323-330 (1995); Kranenburg et al . , FEBS Lett . 367:103-106 (1995)).
- hypophophorylated Rb prevents inappropriate entry of cells into the cell division cycle.
- Phosphorylation of Rb by cyclin-dependent kinases relieves Rb-mediated growth suppression, and allows for cell proliferation (Dowdy et al . , Cell 73:499-511 (1993); Chen et al., Cell 58:1193-1198 (1989)).
- dephosphorylation of Rb during Gl progression induces growth arrest or cell differentiation (Chen et al .
- Rb is dephosphorylated during mitotic exit and Gl entry (Ludlow et al . , Mol . Cell . Biol . 13:367-372 (1993)). This dephosphorylation activates Rb for the ensuing Gl phase of the cell cycle, during which Rb exerts it growth suppressive effects.
- Rb can induce transcriptional activity of wtAR or mARs877t in the presence of DHT, E2 , or HF, and mARe708k in the presence of DHT.
- Rb and ARA70 transciptional activity act synergistically to enhance transciptional activity of ARs.
- the sequence of the cloned Rb gene and the deduced amino acid sequence of the ORF are shown in SEQ ID NO: 7 and SEQ ID NO : 8 , respectively.
- Rb polypeptide is a polypeptide that is substantially homologous to SEQ ID NO: 8, that interacts with the N-terminal domain of AR, and which acts synergistically with ARA70 in enhancing transactivation by AR.
- various eukaryotic cell types including yeast, prostate cells having mutant AR and cells lacking AR, were used to evaluate the ability of the putative androgen coactivators to enhance transactivation by AR. It is expected that in the method of the present invention, any eukaryotic cell could be employed in an assay for AR activity. This feature allows the investigator flexibility in designing assays. As described below, cells were transfected using a calcium phosphate technique. It is expected that the method of the present invention could be practiced using any transfection means including, for example, electroporation or particle bombardment.
- Changes in the level of transactivation by AR can be assessed by any means, including measuring changes in the level of mRNA for a gene under the control of AR, or by quantitating the amount of a particular protein expressed using an antibody specific for a protein, the expression of which is under the control of AR. Most conveniently, transactivation by AR can be assessed by means of a reporter gene .
- a reporter gene is a gene under the control of an androgen receptor, the gene encoding a protein susceptible to quantitation by a colormetric or fluorescent assay.
- a chloramphenicol acetyltransferase or a luciferase gene were used as reporter genes.
- the gene may. either be resident in a chromosome of the host cell, or may be introduced into the host cell by cotransfection with the coactivator gene.
- the following nonlimiting examples are intended to be purely illustrative.
- a human prostate library in pACT2 yeast expression vector (a gift from Dr. S. Elledge) consists of the GAL4 activation domain (GAL4AD, a. a. 768-881) fused with human prostate cDNA.
- GAL4AD GAL4 activation domain
- pSG5 wtAR was constructed as described previously (Yeh and Chang, Proc. Natl. Acad. Sci USA 93:5517-5521, 1996) .
- pGALO-AR wild-type was obtained from D. Chen (University of Massachusetts) .
- pGALO contains the GAL4 DNA binding domain (DBD) .
- pAS2-wtAR or -mAR For construction of pAS2-wtAR or -mAR, the C-terminal fragments (aa 595-918) from wtAR, mARt877s (Dr. S.P. Balk, Beth Israel Hospital, Boston, MA) , or mARe708k (H. Shim, Hyogo Medical College, Japan) were inserted in pAS2 yeast- expression vector (Clontech) .
- Another AR mutant (mARv888m) derived from androgen insensitive syndrome patient, was constructed as previously described (Mowszowicz, et al . Endocrine 1:203-209, 1993).
- pGAL4-VP16 was used to construct a fusion of ARA70.
- pGAL4-VP16 contains the GAL4 DBD linked to the acidic activation domain of VP16.
- pCMX-Gal-N-RB and pCMX-VP16-AR were constructed by inserting fragments Rb (aa 370-928) and AR (aa 590-918) into pCMX-gal-N and pCMX-VP16, respectively. The sequence of construction junction was verified by sequencing.
- pYX-ARA24/Ran was constructed by placing the ARA24 gene under the control of the gal-1 promoter of yeast expression plasmid pYX243 (Ingenus) .
- a cDNA fragment encoding the AR poly-Q stretch and its flanking regions (AR a. a.
- pCMV-antisense ARA24/Ran (ARA24as) expression plasmid was constructed by inserting a 334 -bp Bgl II fragment of ARA24/Ran, which spans 5 ' -untranslated region and the translation start codon of ARA24/Ran (nucleotides 1-334 of SEQ ID NO:5), into pCMV vector in the antisense orientation.
- the MMTV-CAT and MMTV-Luc reporter genes were used for AR transactivation assay.
- pSG5-AR and pSV- ⁇ gal are under the regulation of SV40 promoter and ⁇ - globulin gene intron-1 enhancer.
- p6R-ARQl, p6R-ARQ25, p6R- ARQ49 were kindly provided by Dr. Roger L. Meisfield (Chamberlain, et al . Nucleic Acids Res. 22:3181-3186, 1994)
- pSG5-GAL4DBD-ARA24 was generated by inserting the coding sequence of Gal4DBD-ARA24 hybrid protein into pSG5 vector.
- pVP16-ARN-Ql, pVP16-ARN-Q25 , pVP16 -ARN-Q25 , pVP16- ARN-Q35, pVP16-ARN-Q49 were generated by inserting each poly-Q AR N-terminal domain (a. a.
- pVP16 vector (Clontech) to be expressed as a VP16AD hybrid protein.
- GALOAR plasmid which contains GAL4DBD fused to E region of human AR, was a gift from Dr. D. Chen.
- the pSG5-CAT reporter plasmid (Clontech) contains five GAL4 binding sites upstream of the Elb TATA box, linked to the CAT gene.
- pSG5-AR and pSG5-ARA70 were constructed as previously described (Yeh and Chang, Proc. Natl. Acad. Sci
- Clones used in the two-hybrid system to evaluate the role of Rb in AR transactivation were made by ligating an Rb fragment (aa 371-928) to the DBD of GAL4. Similarly, near full-length (aa 36-918) AR (nAR) and AR-LBD (aa 590- 918) fragments ligated to transcriptional activator VP16.
- a pACT2 -prostate cDNA library was cotransformed into Y190 yeast cells with a plasmid of pAS2mAR (mART877S) which contains GAL4DBD(aa 1-147) fused with the C-terminal domain of this mAR.
- Transformants were selected for growth on SD plates with 3-aminotriazole (25mM) and DHT (lOOnM) lacking histidine, leucine and tryptophan (-3SD plates) .
- Colonies were also filter-assayed for ⁇ -galactosidase activity. Plasmid DNA from positive cDNA clones were found to interact with mtARt877s but not GAL4TR4 was isolated from yeast, amplified in E. coli , and the inserts confirmed by DNA sequencing. To identify clones that interact with the poly-Q region of the N-terminal domain, the AR poly-Q stretch (aa 11-208) was inserted into the pAS2 yeast expression plasmid and cotransformed into Y190 yeast cells with a human brain cDNA library fused to the Gal4 activation domain.
- Transformants were selected for growth on SD plates lacking histidine, leucine and tryptophan and supplemented with 3- aminotriazole (40 mM) .
- ARA54 SEQ ID NO:l
- ARA55 SEQ ID NO : 3
- the missing 5 ' coding region of the ARA54 gene was isolated from H1299 cells using the gene-specific antisense primer shown in SEQ ID NO: 9 and following PCR reaction conditions: 94°C for 1 min, 5 cycles of 94°C for 5 sec ⁇ 72°C for 3 min, 5 cycles of 94°C for 5 sec-70°C for 3 min, then 25 cycles of 94°C for 5 sec-68°C for 3 min.
- the PCR product was subcloned into pT7-Blue vector (Novagen) and sequenced.
- ARA55 was amplified by PCR from the HeLa cell line using an ARA55-specific antisense primer (SEQ ID NO: 10) and the PCR reaction conditions described for isolation of ARA54.
- the in- vi tro translation product is a polypeptide with an apparent molecular mass of 54 ⁇ 2 kDA, consistent with the calculated molecular weight (53.8 kDa) .
- the middle portion of ARA54 (a. a.
- RING finger a cysteine-rich region that may form a zinc finger motif called the RING finger, defined as CX 2 CX 9-27 CXHX 2 CX 2 CX 6-17 CX 2 C (SEQ ID NO: 11), a domain conserved among several human transcriptional factor or proto-oncogeny proteins, including BRCA1 , RING1 , " PML and MEL-18 (Miki et al . , Science 266:66-71 (1994); Borden et al . , EMBO J. 14:1532-1541 (1995); Lovering et al., Proc . Na tl . Acad . Sci .
- ARA54 also contains a second cysteine-rich motif which has a B box like structure located at 43 amino acids downstream from the RING finger motif.
- ARA54 differs from members of the RING finger-B-box family in that it lacks a predicted coiled- coil domain immediately C-terminal to the B box domain, which is highly conserved in the RING finger-B-box family. Therefore, ARA54 may represent a new subgroup of this family.
- the full-length human ARA55 has an open reading frame that encodes a 444 aa polypeptide (SEQ ID NO: 4) with a predicted molecular weight of 55 kD that ARA55 shares 91% homology with mouse hic5.
- Human ARA55 has four LIM motifs in the C-terminal region.
- An LIM motif is a cysteine-rich zinc-binding motif with consensus sequence: CX 2 CX 16 _ 23 HX 2 CX 2 CX 2 CX 16-21 CX 2 (C,H,D) (SEQ ID NO:12) (Sadler, et al . , J. Cell Biol . 119:1573-1587(1992)).
- LIM motif Although the function of the LIM motif has not been fully defined, some data suggest that it may play a role in protein-protein interaction (Schmeichel & Beckerle, Cell 79:211-219, 1994). Among all identified SR associated proteins, only ARA55 and thyroid hormone interacting protein 6 (Trip 6) (Lee, et al . Mol . Endocrinol . 9:243-254 (1995)) have LIM motifs. A clone that showed strong interaction with the poly-Q bait was identified and subsequently subjected to sequence analysis.
- This clone contains 1566 bp insert (SEQ ID NO: 5) with an open reading frame encoding a 216 aa polypeptide (SEQ ID NO: 6) with a calculated molecular weight of 24 kDa .
- GenBank sequence comparison showed that this clone has the same open reading frame sequence as Ran/TC4 , an abundant ras-like small GTPase involved in nucleocytoplasmic transport that is found in a wide variety of cell types (Beddow et al . , Proc. Natl. Acad. Sci. U.S.A. 92:3328-333.2, 1995) . Accordingly, the factor was designated ARA24/Ran.
- the cDNA sequence of the ARA24 clone (SEQ ID NO: 5) (GenBank accession number AF052578) is longer than that of the published ORF for human Ran, in that it includes 24 and 891 bp of 5'- and 3 ' -untranslated regions, respectively.
- RNA The total RNA (25 ⁇ g) was fractionated on a 1% formaldehyde-MOPS agarose gel, transferred onto a Hybond-N nylon membrane (Amersham) and prehybridized.
- a probe corresponding to the 900 bp C-terminus of ARA55 or an ARA54 -specific sequence was 32 P-labeled in vi tro using Random Primed DNA Labeling Kit (Boehringer-Mannheim) according to the manufacture's protocol and hybridized overnight. After washing, the blot was exposed and quantified by Molecular Dynamics Phosphorlmager . ⁇ -actin was used to monitor the amount of total RNA in each lane .
- Northern blot analysis indicated the presence of a 2 kb ARA55 transcript in Hela and prostate PC3 cells.
- the transcript was not detected in other tested cell lines, including HepG2 , H1299, MCF7, CHO, PC12, P19, and DU145 cells.
- the ARA54 transcript was found in H1299 cells, as well as in prostate cancer cell lines PC3 and LNCaP.
- Lysates from in-vi tro translated full-length of AR and ARA54 were incubated with or without 10 "8 M DHT in the modified RIPA buffer (50mM Tris-HCL pH 7.4, 150mM NaCl, 5mM EDTA, 0.1% NP40, ImM PMSF, aprotinin, leupeptin, pepstatin, 0.25% Na-deoxycholate, 0.25% gelatin) and rocked at 4°C for
- ARA54 and AR were found in a complex when immunoprecipitated in the presence of 10 ⁇ 8 M DHT, but not in the absence of DHT. This result suggests that ARA54 interacts with AR in an androgen-dependent manner.
- ST-Rb 1-928 was expressed and purified from E. coli. strain Bl21pLys as described recently (Zarkowska & Mittnacht, J. Biol. Chem. 272:12738-12746, 1997). Approximately 2 ⁇ g of His-tag column purified baculovirus AR was mixed with GST- loaded glutathione-Sepharose beads in 1 ml of NET-N (20 mM Tris-HCL(pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% (v/v) Noniodet P-40) and incubated with gentle rocking for 3 hr at 4°C.
- NET-N 20 mM Tris-HCL(pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% (v/v) Noniodet P-40
- the clarified supernatant was mixed with GST-Rb- loaded glutathione-Sepharose beads in the presence or absence of 10 mM DHT and incubated for an additional 3 hr with gentle rocking at 4°C.
- the pelleted beads were washed 5 times with NET-N, mixed with SDS-sample buffer, boiled, and the proteins separated by electrophoresis on a 7.5% polyacrylamide gel.
- a Western blot of the gel was incubated with anti-AR polyclonal antibody NH27 and developed with alkaline phosphatase-conjugated secondary antibodies .
- AR was coprecipitated with GST-Rb, but not GST alone, indicating that AR and Rb are associated in a complex together.
- Human prostate cancer DU145 or PC3 cells, or human - lung carcinoma cells NCI H1299 were grown in Dulbecco's minimal essential medium (DMEM) containing penicillin (25U/ml) , streptomycin (25 ⁇ g/ml) , and 5% fetal calf serum (FCS) .
- DMEM Dulbecco's minimal essential medium
- FCS fetal calf serum
- Phenol red- free and serum- free media were used on the experiments employing E2 or TGF ⁇ , respectively.
- a ⁇ -galactosidase expression plasmid, pCMV- ⁇ -gal was used as an internal control for transfection efficiency.
- Cells were transfected using the calcium phosphate technique (Yeh, et al . Molec . Endocrinol . 8:77-88, 1994). The medium was changed 24 hr posttransfection and the cells treated with either steroid hormones or hydroxyflutamide , and cultured for an additional 24 hr. Cells were harvested and assayed for CAT activity after the cell lysates were normalized by using ⁇ -galactosidase as an internal control. Chloramphenicol acetyltransferase (CAT) activity was visualized by Phosphorlmager (Molecular Dynamics) and quantitated by ImageQuant software (Molecular Dynamics) .
- CAT Chloramphenicol acetyltransferase
- the mammalian two-hybrid system employed was essentially the protocol of Clontech (California) , with the following modifications.
- the GAL4DBD (a. a. 1-147) was fused to pSG5 under the control of an SV40 promoter, and named pGALO .
- the hinge and LBD of wtAR were then inserted into pGALO .
- the VP16 activation domain was fused to pCMX under the control of a CMV promoter, and designated pCMX-
- ARA54 functions as a coactivator relatively specific for AR-mediated transcription. ARA54 induces the transcriptional activity of AR and PR by up to 6 fold and 3-5 fold, respectively. In contrast, ARA54 showed only marginal effects (less than 2 fold) on GR and ER in DU145 cells. These data suggest that ARA54 is less specific to AR as relative to ARA70, which shows higher specificity to AR. However, we can not rule out the possibility that
- ARA54 might be more general to other steroid receptors in other cell types under different conditions.
- the positive control is able to enhance ' the AR transcriptional activity in the presence of 10 ⁇ 9 - 10 "7 M E2 and 10 ⁇ 7 - 10 "5 HF, that matches well with previous reports (Yeh, PNAS, Miyamoto, PNAS) .
- CAT activity in DU145 cells cotransfected with a plasmid encoding the hormone binding domain of wtAR fused to the GAL4 DBD (GALOAR) and a plasmid encoding full-length ARA55 fused to the activation domain of VP16 (VP16-ARA55) was significantly induced by the cotransfection of VP16- ARA55 and GALOAR in the presence of 10 nM DHT, but not induced by E2 or HF .
- Combination of GAL0 empty vector and VP16-ARA55 did not show any CAT activity.
- Combination of GALOAR and VP16 vector showed negligible CAT activity.
- Transient transfection assays were conducted to investigate the role of ARA55 in the transactivation activity of AR.
- DU145 cells were cotransfected with MMTV- CAT reporter, increasing amounts of ARA55 and wtAR under eukaryotic promoter control.
- Ligand- free AR has minimal MMTV-CAT reporter activity in the presence or absence of ARA55.
- ARA55 alone also has only minimal reporter activity
- Addition of 10 nM DHT resulted in 4.3 fold increase of AR transcriptional activity and ARA55 further increased this induction by 5.3 fold (from 4.3 fold to 22.8 fold) in a dose-dependent manner.
- the induced activity reached a plateau at the ratio of AR:ARA55 to 1:4.5.
- the mARt877s receptor is found in LNCaP cells and/or advanced prostate cancers and has a point mutation at codon 877 (Thr to Ser) (Gaddipati et al . , Cancer Res . 54:2861-2864 (1994); Veldscholte et al . , Biochem . Biophys . Commun . 173:534-540 (1990) ) .
- the mARe708k receptor has a point mutation at codon 708 (Glu to Lys) , was isolated by a yeast genetic screening and exhibits reduced sensitivity to HF and E2 relative to wtAR(Wang, C. , PhD thesis of Universi ty of Wisconsin -Madison (1997) ) .
- the transcriptional activities of wtAR, mARt877s, and mARe708k are induced by DHT (10 "11 to 10" 8 M) .
- ARA55 enhanced the transactivation of all three receptors by 4-8 fold. In the presence of E2 or HF, wtAR responded marginally only at higher concentrations (10 ⁇ 7 M for E2 and 10 ⁇ 5 M for HF) .
- Cotransfection of wtAR with ARA55 at a 1:4.5 ratio increases AR transcriptional activity at 10 ⁇ 8 -10 ⁇ 7 M for E2 or 10 ⁇ 6 to 10 ⁇ 5 M for HF.
- the LNCaP mAR responded much better to E2 and HF and ARA55 significantly enhanced its transcriptional activity.
- ARA55 may be needed for the proper or maximal DHT- , E2-, or HF-mediated AR transcriptional activity.
- ARA55 The effect of ARA55 on transcriptional activation by GR, PR, and ER was tested in DU145 cells.
- ARA55 is relatively specific to AR, although it may also enhance GR and PR to a lesser degree, and has only a marginal effect on ER.
- ARA70 shows much higher specificity to AR than ARA55, relative to the other tested steroid receptors. Although ARA55 enhances AR-mediated transcription to a greater degree than GR- , PR-, or ER-mediated transcription, it appears to be less specific than ARA70.
- TGF ⁇ may increase AR transcriptional activity via induction of ARA55 in prostate may represent the first evidence to link a negative regulatory protein function in a positive manner, by inducing the transcriptional activity of AR, the major promoter for the prostate tumor growth.
- ARA55 ability of ARA55 to induce transcriptional activity of both wtAR and mARt877s in the presence of DHT, E2 , and HF suggests an important role for ARA55 in the progression of prostate cancer and the development of resistance to hormonal therapy. Evaluation of molecules that interfere with the function of ARA55 may aid in the identification of potential chemotherapeutic pharmaceuticals.
- ARA24/Ran Human small lung carcinoma H1299 cell line, which has no endogenous AR protein, were transfected with AR and ARA24/Ran. Because ARA24/Ran is one of the most abundant and ubiquitously expressed proteins in various cells, both sense and antisense ARA24/Ran mammalian expression plasmids were tested. Overexpression of sense ARA24/Ran did not significantly enhance the AR transactivation, a result that is not surprising, in view of the abundance of endogenous ARA24/RAN. However, expression of antisense ARA24/Ran (ARA24as) markedly decreased DHT- induced CAT activity in a dose dependent manner.
- the affinity between ARA24/Ran and AR is inversely related to the length of AR poly-Q stretch. AR transactivation decreases with increasing AR poly-Q length. Reciprocal two-hybrid assays with exchanged fusion partners, Gal4DBD-ARA24/Ran and VP16AD-ARNS (a. a. 34-555 with poly-Q lengths of 1, 25, 35, 49 residues) were conducted using mammalian CHO cells. These results consistently show that the affinity between ARA24/Ran and AR poly-Q region is inversely correlated with AR poly-Q length in both yeast and mammalian CHO cells.
- ARA24/Ran The regulation of AR transactivation by ARA24/Ran correlates with their affinity. These results suggest that ARA24/Ran could achieve differential transactivation of AR, with ARs having different poly-Q length could existing in a single cell or cell system.
- ARA24as was again used in the ARE-Luc transfection assays to address the role of AR poly- Q length in the regulation of AR by ARA24/Ran. ARs of poly-Q lengths 1, 25, and 49 residues, and increasing amounts (1, 2, and 4 ⁇ g) of ARA24as expression vectors were co-transfected with equal amounts of reporter plasmid (pMMTV-Luc) in CHO cells.
- Rb and AR are unique in the following ways: first, the interaction is androgen- independent and binding is specific but relatively weak as compared to other AR associated protein, such as ARA70 (3 fold vs. 12 fold induced CAT activity in mammalian two- hybrid assay, data not shown) . Second, unlike most identified steroid receptor associated proteins that bind to C-terminal domain of steroid receptor, Rb binds to N- terminal domain of AR. Third, no interaction occurred between Rb and ARA70, two AR associated proteins in DU145 cells.
- AR associated protein such as ARA70 (3 fold vs. 12 fold induced CAT activity in mammalian two- hybrid assay, data not shown) .
- ARA70 3 fold vs. 12 fold induced CAT activity in mammalian two- hybrid assay, data not shown
- DU145 cells containing mutated Rb (Singh et al . , Na ture 374: 562-565 (1995)) were cultured with charcoal- stripped FCS in the presence or absence of 1 nM DHT. No AR transcriptional activity was observed in DU145 cells transiently transfected with wild type AR and Rb at the ratio of 1:3 in the absence of DHT. When However, AR transcriptional activity could be induced 5-fold when wild type AR was expressed in the presence of 1 nM DHT.
- Cotransfection of Rb with AR can further enhance the AR transcriptional activity from 5-fold to 21-fold in the presence of 1 nM DHT.
- cotransfection of ARA70 the first identified AR coactivator
- DU145 cells transcriptional activity from 5-fold to 36-fold.
- the induction of AR transcriptional activity was synergistically increased from 5-fold to 64-fold.
- Rb and ARA70 can induce transcriptional activity of both wild type AR and mutated AR that occur in many prostate tumors may also argue strongly the importance of Rb and ARA70 in normal prostate as well as prostate tumor. Also, the differential induction of DHT vs. E2/HF may suggest the position of 708 in AR may play vital role for the recognition of androgen vs anti- androgens to AR.
- Rb and ARA70 are more specific coactivators for AR in prostate DU145 cells.
- Rb in cell cycle control is related essentially to its ability to bind to several proteins, thus modulating their activity.
- many cellular proteins have been reported which bind to Rb (Weinberg, R.A., Cell 81:323-330 (1995)) . These include a number of transcription factors, a putative regulator of ras, a nuclear structural protein, a protein phosphatase, and several protein kinases. Whether all of these proteins actually complex, and are regulated by Rb, in cells remains to be seen.
- Rb can bind to AR and induce the AR transcriptional activity.
- this is the first demonstration of a negative growth regulatory protein functioning in a positive manner, by initiating transcription via a signal transduction mechanism involving binding to a nuclear receptor.
- these data suggest a previously undescribed function for Rb which underscores the importance of this protein in regulating transcription by direct binding to transcription factor, but this protein can also regulate transcription by stimulating at least one type of signal transduction mechanism.
Abstract
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JP2000560250A JP2002520056A (en) | 1998-07-17 | 1999-07-16 | Androgen receptor coactivator |
CA002335101A CA2335101A1 (en) | 1998-07-17 | 1999-07-16 | Androgen receptor coactivators |
AU51069/99A AU771564B2 (en) | 1998-07-17 | 1999-07-16 | Androgen receptor coactivators |
EP99935630A EP1098976A2 (en) | 1998-07-17 | 1999-07-16 | Androgen receptor coactivators |
AU2004203835A AU2004203835B2 (en) | 1998-07-17 | 2004-08-11 | Androgen receptor coactivators |
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US60/100,243 | 1998-09-14 |
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JP (1) | JP2002520056A (en) |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2002142769A (en) * | 2000-11-07 | 2002-05-21 | Japan Science & Technology Corp | Method for obtaining gene specific to neurogenesis of vertebrate |
EP1227150A2 (en) * | 2001-01-17 | 2002-07-31 | Veterans General Hospital | Androgen receptor complex-associated protein |
DE10121710A1 (en) * | 2001-05-04 | 2002-11-14 | Jenapharm Gmbh | Assessing hormonal effects of compounds, useful in testing drugs, from effect on interaction of nuclear receptor and co-modulator, also diagnosing diseases caused by improper co-modulation |
EP1270591A1 (en) * | 2001-06-25 | 2003-01-02 | Okazaki National Research Institutes | Transgenic cells and a method for detecting disruptors for androgens by using said transgenic cells |
EP1386157A2 (en) * | 2001-04-06 | 2004-02-04 | University of Rochester | Suppression of androgen receptor transactivation through new pathways to ar and ar coactivators and repressors |
EP1400596A1 (en) * | 2002-07-25 | 2004-03-24 | Taipei-Veterans General Hospital | Androgen receptor complex-associated protein |
EP1532163A2 (en) * | 2002-06-06 | 2005-05-25 | University of Rochester | Androgen receptor coregulators |
EP1955700A2 (en) | 1999-09-30 | 2008-08-13 | Hollis-Eden Pharmaceuticals Inc. | Therapeutic treatment of androgen receptor driven conditions |
US7993475B2 (en) | 2004-06-17 | 2011-08-09 | Nof Corporation | Firing agent for gas generating device |
Citations (1)
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WO1997044490A1 (en) * | 1996-05-23 | 1997-11-27 | Wisconsin Alumni Research Foundation | Specific co-activator for human androgen receptor |
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WO1997044490A1 (en) * | 1996-05-23 | 1997-11-27 | Wisconsin Alumni Research Foundation | Specific co-activator for human androgen receptor |
Non-Patent Citations (4)
Title |
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HILLIER ET AL.: "WashU-Merck EST Project 1997" EMBL ACC NO: AA448471, 10 June 1997 (1997-06-10), XP002121287 * |
KANG ET AL.: "Cloning and characterization of human prostate coactivator ARA54, a novel protein that associates with the androgen receptor" THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 13, 26 March 1999 (1999-03-26), pages 8570-8576, XP002121288 * |
MIYAMOTO ET AL.: "Promotion of agonist activity of antiandrogens by the androgen receptor coactivator, ARA70, in human prostate cancer DU145 cells" PROC. NATL. ACAD. SCI. USA, vol. 95, June 1998 (1998-06), pages 7379-7384, XP002121286 cited in the application * |
YEH ET AL.: "Cloning and characterization of a specific coactivator, ARA70, for the androgen receptor in human prostate cells" PROC. NATL. ACAD. SCI. USA, vol. 93, May 1996 (1996-05), pages 5517-5521, XP002121285 cited in the application * |
Cited By (14)
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EP1955700A2 (en) | 1999-09-30 | 2008-08-13 | Hollis-Eden Pharmaceuticals Inc. | Therapeutic treatment of androgen receptor driven conditions |
JP2002142769A (en) * | 2000-11-07 | 2002-05-21 | Japan Science & Technology Corp | Method for obtaining gene specific to neurogenesis of vertebrate |
EP1227150A2 (en) * | 2001-01-17 | 2002-07-31 | Veterans General Hospital | Androgen receptor complex-associated protein |
EP1227150A3 (en) * | 2001-01-17 | 2002-10-09 | Veterans General Hospital | Androgen receptor complex-associated protein |
CN100447157C (en) * | 2001-01-17 | 2008-12-31 | 台北荣民总医院 | Androgen receptor compound relative protein |
US6974683B2 (en) | 2001-01-17 | 2005-12-13 | Veterans General Hospital | Nucleic acid encoding androgen receptor complex-associated protein |
EP1386157A4 (en) * | 2001-04-06 | 2005-06-22 | Univ Rochester | Suppression of androgen receptor transactivation through new pathways to ar and ar coactivators and repressors |
EP1386157A2 (en) * | 2001-04-06 | 2004-02-04 | University of Rochester | Suppression of androgen receptor transactivation through new pathways to ar and ar coactivators and repressors |
DE10121710A1 (en) * | 2001-05-04 | 2002-11-14 | Jenapharm Gmbh | Assessing hormonal effects of compounds, useful in testing drugs, from effect on interaction of nuclear receptor and co-modulator, also diagnosing diseases caused by improper co-modulation |
EP1270591A1 (en) * | 2001-06-25 | 2003-01-02 | Okazaki National Research Institutes | Transgenic cells and a method for detecting disruptors for androgens by using said transgenic cells |
EP1532163A2 (en) * | 2002-06-06 | 2005-05-25 | University of Rochester | Androgen receptor coregulators |
EP1532163A4 (en) * | 2002-06-06 | 2007-02-21 | Univ Rochester | Androgen receptor coregulators |
EP1400596A1 (en) * | 2002-07-25 | 2004-03-24 | Taipei-Veterans General Hospital | Androgen receptor complex-associated protein |
US7993475B2 (en) | 2004-06-17 | 2011-08-09 | Nof Corporation | Firing agent for gas generating device |
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JP2002520056A (en) | 2002-07-09 |
AU2004203835B2 (en) | 2007-12-13 |
AU2004203835A1 (en) | 2004-09-09 |
AU771564B2 (en) | 2004-03-25 |
AU5106999A (en) | 2000-02-07 |
EP1098976A2 (en) | 2001-05-16 |
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CA2335101A1 (en) | 2000-01-27 |
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