WO2000003021A3 - Metabolically engineered microbial cell comprising a modified redox activity - Google Patents

Metabolically engineered microbial cell comprising a modified redox activity Download PDF

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Publication number
WO2000003021A3
WO2000003021A3 PCT/DK1999/000398 DK9900398W WO0003021A3 WO 2000003021 A3 WO2000003021 A3 WO 2000003021A3 DK 9900398 W DK9900398 W DK 9900398W WO 0003021 A3 WO0003021 A3 WO 0003021A3
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WIPO (PCT)
Prior art keywords
enzyme activity
metabolite
expressible enzyme
microbial cell
production
Prior art date
Application number
PCT/DK1999/000398
Other languages
French (fr)
Other versions
WO2000003021A2 (en
Inventor
Jens Nielsen
Torben Lauesgaard Nissen
Morten C Kielland-Brandt
Original Assignee
Jens Nielsen
Torben Lauesgaard Nissen
Kielland Brandt Morten C
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jens Nielsen, Torben Lauesgaard Nissen, Kielland Brandt Morten C filed Critical Jens Nielsen
Priority to CA002379034A priority Critical patent/CA2379034A1/en
Priority to EP99932683A priority patent/EP1097217A2/en
Priority to AU48979/99A priority patent/AU4897999A/en
Publication of WO2000003021A2 publication Critical patent/WO2000003021A2/en
Publication of WO2000003021A3 publication Critical patent/WO2000003021A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The present invention concerns a microbial cell comprising a first expressible enzyme activity, e.g., pyridine nucleotide transhydrogenase from Azotobacter vinelandii which, when expressed in said microbial cell, is controlling an intracellular redox system of said cell, said first expressible enzyme activity in said microbial cell being operably linked to an expression signal not natively associated with said first expressible enzyme activity. The expression of said first expressible enzyme activity is operably linked to an increased production of a first metabolite. The cell is preferably a yeast cell and may also comprise a further expressible enzyme activity, e.g., glutamate synthase and/or glutamine synthetase, said further expressible enzyme activity, when expressed, mediating a first biosynthetic reaction resulting in production of a first metabolite, said further expressible enzyme activity, when expressed at an increased level, resulting in an increased production of said first metabolite, said increased expression of said further expressible enzyme activity and/or said increased production of said first metabolite being operably linked to an increased expression of said first expressible enzyme activity. There is also provided a microbial cell wherein said expression of said first expressible enzyme activity is operably linked to an increased production of a first metabolite and a decreased production of a second metabolite. Cells according to the invention are useful in the production of a metabolite such as ethanol or glycerol.
PCT/DK1999/000398 1998-07-10 1999-07-12 Metabolically engineered microbial cell comprising a modified redox activity WO2000003021A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002379034A CA2379034A1 (en) 1998-07-10 1999-07-12 Metabolically engineered microbial cell comprising a modified redox activity
EP99932683A EP1097217A2 (en) 1998-07-10 1999-07-12 Metabolically engineered microbial cell comprising a modified redox activity
AU48979/99A AU4897999A (en) 1998-07-10 1999-07-12 Metabolically engineered microbial cell comprising a modified redox activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199800968 1998-07-10
DKPA199800968 1998-07-10

Publications (2)

Publication Number Publication Date
WO2000003021A2 WO2000003021A2 (en) 2000-01-20
WO2000003021A3 true WO2000003021A3 (en) 2000-04-20

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PCT/DK1999/000398 WO2000003021A2 (en) 1998-07-10 1999-07-12 Metabolically engineered microbial cell comprising a modified redox activity

Country Status (4)

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EP (1) EP1097217A2 (en)
AU (1) AU4897999A (en)
CA (1) CA2379034A1 (en)
WO (1) WO2000003021A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1325633C (en) * 2005-08-08 2007-07-11 天津大学 Glycerol channel protein gene deleted brewing microzyme strain capable of reducing glycerol output and increasing ethanol output and construction method thereof
CN100395339C (en) * 2006-02-28 2008-06-18 中国人民解放军第二军医大学 Method for expressing hepatitis C virus envelope protein E2 by mammal cell with high efficient secretion
WO2009053489A1 (en) * 2007-10-27 2009-04-30 Evonik Degussa Gmbh Fermentative production of alpha-ketoglutaric acid
CA2779262C (en) 2008-10-31 2021-09-07 Gevo, Inc. Engineered microorganisms capable of converting pyruvate to isobutanol under anaerobic conditions
EP2277989A1 (en) 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
US9605285B2 (en) 2011-01-25 2017-03-28 Cargill, Incorporated Compositions and methods for succinate production
WO2014018755A1 (en) * 2012-07-25 2014-01-30 Cargill Incorporated Yeast cells having nadp(h)-dependent reductive tca pathway from pyruvate to succinate
US9850507B2 (en) 2012-07-25 2017-12-26 Cargill, Incorporated Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P)+ transhydrogenase enzyme
CN103627698A (en) * 2013-12-05 2014-03-12 江南大学 Breeding of acetoin high-tolerance bacterial strain and acetoin fermentation production with bacterial strain
KR102384227B1 (en) 2015-05-18 2022-04-07 삼성전자주식회사 Genetically engineered yeast cell having increased production of NADPH, method for increasing NADPH levels in a yeast cell, method for preparing the yeast cell and method for producing a lactate using the yeast cell
CN107354180B (en) * 2016-05-09 2021-09-07 中国石化扬子石油化工有限公司 Regulation and control method for producing succinic acid by actinobacillus succinogenes
CN113564184B (en) * 2021-07-16 2023-04-18 昆明理工大学 Gastrodia elata glutamine synthetase gene and application thereof

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Publication number Publication date
CA2379034A1 (en) 2000-01-20
EP1097217A2 (en) 2001-05-09
WO2000003021A2 (en) 2000-01-20
AU4897999A (en) 2000-02-01

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