WO2000000593A3 - Sphingolipid-desaturase - Google Patents

Sphingolipid-desaturase Download PDF

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Publication number
WO2000000593A3
WO2000000593A3 PCT/DE1999/001859 DE9901859W WO0000593A3 WO 2000000593 A3 WO2000000593 A3 WO 2000000593A3 DE 9901859 W DE9901859 W DE 9901859W WO 0000593 A3 WO0000593 A3 WO 0000593A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzymes
sphingolipids
capnoids
sphingobase
dna sequences
Prior art date
Application number
PCT/DE1999/001859
Other languages
German (de)
French (fr)
Other versions
WO2000000593A2 (en
Inventor
Ernst Heinz
Ulrich Zaehringer
Petra Sperling
Hermann Schmidt
Original Assignee
Gvs Ges Fuer Erwerb Und Verwer
Ernst Heinz
Ulrich Zaehringer
Petra Sperling
Hermann Schmidt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gvs Ges Fuer Erwerb Und Verwer, Ernst Heinz, Ulrich Zaehringer, Petra Sperling, Hermann Schmidt filed Critical Gvs Ges Fuer Erwerb Und Verwer
Priority to AU54075/99A priority Critical patent/AU5407599A/en
Publication of WO2000000593A2 publication Critical patent/WO2000000593A2/en
Publication of WO2000000593A3 publication Critical patent/WO2000000593A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to enzymes which selectively introduce a double bond in the sphingobase of the ceramide residual of sphingolipids and of capnoids. The invention also relates to DNA sequences which code for these enzymes, to the use of the enzymes and DNA sequences for modifying the content and/or the structure of sphingolipids, the catabolites thereof and/or their synthetic subsequent products in transgenic cells and/or organisms. In addition, a method is provided for producing sphingolipids and capnoids with an unsaturated sphingobase.
PCT/DE1999/001859 1998-06-27 1999-06-25 Sphingolipid-desaturase WO2000000593A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU54075/99A AU5407599A (en) 1998-06-27 1999-06-25 Sphingolipid-desaturase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19828850A DE19828850A1 (en) 1998-06-27 1998-06-27 New sphingolipid desaturase that selectively introduces double bond into sphingolipids and capnoids
DE19828850.6 1998-06-27

Publications (2)

Publication Number Publication Date
WO2000000593A2 WO2000000593A2 (en) 2000-01-06
WO2000000593A3 true WO2000000593A3 (en) 2000-08-10

Family

ID=7872312

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/001859 WO2000000593A2 (en) 1998-06-27 1999-06-25 Sphingolipid-desaturase

Country Status (3)

Country Link
AU (1) AU5407599A (en)
DE (1) DE19828850A1 (en)
WO (1) WO2000000593A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106754769A (en) * 2016-12-01 2017-05-31 中国科学院华南植物园 A kind of tomato Inappropriate ADH syndrome gene and application

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10030976A1 (en) 2000-06-30 2002-01-10 Basf Ag Production of unsaturated fatty acids, useful e.g. in nutrition, cosmetics or pharmaceuticals, in organisms transformed with Physcomitrella patens delta-6-desaturase nucleic acid
ES2523856T3 (en) 2000-12-22 2014-12-02 Lpath, Inc. Sphingosine-1-phosphate antibodies for the treatment of diseases associated with high concentrations of sphingolipids
JP4737531B2 (en) * 2005-12-05 2011-08-03 サントリーホールディングス株式会社 Method for producing ceramide using transformed yeast
US9274130B2 (en) 2006-05-31 2016-03-01 Lpath, Inc. Prevention and treatment of pain using antibodies to lysophosphatidic acid
US9274129B2 (en) 2006-05-31 2016-03-01 Lpath, Inc. Methods and reagents for detecting bioactive lipids
US20080138334A1 (en) 2006-05-31 2008-06-12 Sabbadini Roger A Immune-Derived Moieties Reactive Against Bioactive Lipids, and Methods of Making and Using Same
ES2524059T3 (en) 2006-10-27 2014-12-03 Lpath, Inc. Compositions and procedures of binding to sphingosine-1-phosphate
US8871202B2 (en) 2008-10-24 2014-10-28 Lpath, Inc. Prevention and treatment of pain using antibodies to sphingosine-1-phosphate
US10045499B2 (en) * 2012-05-24 2018-08-14 Iowa State University Research Foundation, Inc. Arabidopsis nonhost resistance gene(s) and use thereof to engineer disease resistant plants

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021022A2 (en) * 1994-12-30 1996-07-11 Rhone-Poulenc Agrochimie Production of gamma linolenic acid by a δ6-desaturase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021022A2 (en) * 1994-12-30 1996-07-11 Rhone-Poulenc Agrochimie Production of gamma linolenic acid by a δ6-desaturase

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
NAPIER, J. A. (1) ET AL: "Cytochrome b5 fusion proteins: A new class of higher plant fatty acid desaturases.", JOURNAL OF EXPERIMENTAL BOTANY, (MAY, 1998) VOL. 49, NO. SUPPL., PP. 15. MEETING INFO.: THE SOCIETY FOR EXPERIMENTAL BIOLOGY ANNUAL MEETING LONDON, ENGLAND, UK MARCH 22-27, 1998 SOCIETY FOR EXPERIMENTAL BIOLOGY., XP002132573 *
SCHMIDT, HERMANN (1) ET AL: "Purification and PCR-based cDNA cloning of a plastidial n-6 desaturase.", PLANT MOLECULAR BIOLOGY, (1994) VOL. 26, NO. 2, PP. 631-642., XP002132574 *
SPERLING, PETRA ET AL: "A cytochrome-b5-containing fusion protein similar to plant acyl lipid desaturases", EUR. J. BIOCHEM. (1995), 232(3), 798-805, XP002132571 *
SPERLING, PETRA ET AL: "A sphingolipid desaturase from higher plants. Identification of a new cytochrome b5 fusion protein", J. BIOL. CHEM., 273(44), 28590-28596, 30 October 1998 (1998-10-30), XP002132572 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106754769A (en) * 2016-12-01 2017-05-31 中国科学院华南植物园 A kind of tomato Inappropriate ADH syndrome gene and application
CN106754769B (en) * 2016-12-01 2019-08-23 中国科学院华南植物园 A kind of tomato Inappropriate ADH syndrome gene and application

Also Published As

Publication number Publication date
WO2000000593A2 (en) 2000-01-06
AU5407599A (en) 2000-01-17
DE19828850A1 (en) 1999-12-30

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