WO1999067402A3 - Methods to screen herbicidal compounds utilizing air synthetase from arabidopsis thaliana - Google Patents

Methods to screen herbicidal compounds utilizing air synthetase from arabidopsis thaliana Download PDF

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Publication number
WO1999067402A3
WO1999067402A3 PCT/EP1999/004309 EP9904309W WO9967402A3 WO 1999067402 A3 WO1999067402 A3 WO 1999067402A3 EP 9904309 W EP9904309 W EP 9904309W WO 9967402 A3 WO9967402 A3 WO 9967402A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
screen
air synthetase
arabidopsis thaliana
herbicidal compounds
Prior art date
Application number
PCT/EP1999/004309
Other languages
French (fr)
Other versions
WO1999067402A2 (en
Inventor
Eric Russell Ward
Charles David Guyer
Sharon Lee Potter
Venkiteswaran Subramanian
Eric Walters
Original Assignee
Novartis Ag
Novartis Erfind Verwalt Gmbh
Eric Russell Ward
Charles David Guyer
Sharon Lee Potter
Venkiteswaran Subramanian
Eric Walters
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag, Novartis Erfind Verwalt Gmbh, Eric Russell Ward, Charles David Guyer, Sharon Lee Potter, Venkiteswaran Subramanian, Eric Walters filed Critical Novartis Ag
Priority to JP2000556042A priority Critical patent/JP2002518054A/en
Priority to EP99936458A priority patent/EP1090135A2/en
Priority to CA002329690A priority patent/CA2329690A1/en
Priority to AU51552/99A priority patent/AU5155299A/en
Publication of WO1999067402A2 publication Critical patent/WO1999067402A2/en
Publication of WO1999067402A3 publication Critical patent/WO1999067402A3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention discloses methods to screen chemicals for herbicidal activity using recombinantly produced enzymes having AIR synthetase activity, and the use thereby to identify herbicidal chemicals to suppress the growth of undesired vegetation. Furthermore, the present invention provides methods for the development of herbicide tolerance in plants, plant tissues, plant seeds, and plant cells using genes encoding enzymes having AIR synthetase activity, and methods of using such transgenic plants to selectively suppress weed growth in crop fields.
PCT/EP1999/004309 1998-06-24 1999-06-22 Methods to screen herbicidal compounds utilizing air synthetase from arabidopsis thaliana WO1999067402A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000556042A JP2002518054A (en) 1998-06-24 1999-06-22 Method for screening herbicidal compounds using AIR synthetase from Arabidopsis thaliana
EP99936458A EP1090135A2 (en) 1998-06-24 1999-06-22 Methods to screen herbicidal compounds utilizing air synthetase from arabidopsis thaliana
CA002329690A CA2329690A1 (en) 1998-06-24 1999-06-22 Methods to screen herbicidal compounds utilizing air synthetase from arabidopsis thaliana
AU51552/99A AU5155299A (en) 1998-06-24 1999-06-22 Methods to screen herbicidal compounds utilizing AIR synthetase from Arabidopsis thaliana

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10389598A 1998-06-24 1998-06-24
US09/103,895 1998-06-24

Publications (2)

Publication Number Publication Date
WO1999067402A2 WO1999067402A2 (en) 1999-12-29
WO1999067402A3 true WO1999067402A3 (en) 2000-03-09

Family

ID=22297594

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/004309 WO1999067402A2 (en) 1998-06-24 1999-06-22 Methods to screen herbicidal compounds utilizing air synthetase from arabidopsis thaliana

Country Status (6)

Country Link
EP (1) EP1090135A2 (en)
JP (1) JP2002518054A (en)
CN (1) CN1316004A (en)
AU (1) AU5155299A (en)
CA (1) CA2329690A1 (en)
WO (1) WO1999067402A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5482299A (en) * 1998-08-12 2000-03-06 Maxygen, Inc. Dna shuffling to produce herbicide selective crops
EP1407270A4 (en) * 2001-05-30 2005-05-25 Icoria Inc Methods for the identification of inhibitors of 3-oxo-5-alpha-steroid 4-dehydrogenase expression or activity in plants

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ABELL ET AL: "Biochemical aproaches to herbicide discovery: advances in enzyme target identification and inhibitor design", WEED SCIENCE,US,WEED SCIENCE SOCIETY OF AMERICA, CHAMPAIGN, IL, vol. 44, pages 734-742, XP002094170, ISSN: 0043-1745 *
SCHRIMSHER J L ET AL.: "Isolation of a multifunctional protein with aminoimidazole ribonucleotide synthetase, glycinamide ribonucleotide synthetase, and glycinamide ribonucleotide transformylase activities", BIOCHEMISTRY, vol. 25, 1986, pages 4356 - 4365, XP002124569 *
SENECOFF J F ET AL: "Isolating the Arabidopsis thaliana genes for de novo purine synthesis by suppression of Escherichia coli mutants. I. 5' Phosphoribosyl-5-aminoimidazole synthetase.", PLANT PHYSIOLOGY, (1993 JUN) 102 (2) 387-99. JOURNAL CODE: P98., XP002124568 *
STEMMER W P C: "RAPID EVOLUTION OF A PROTEIN IN VITRO BY DNA SHUFFLING", NATURE,GB,MACMILLAN JOURNALS LTD. LONDON, vol. 370, pages 389-391, XP002912216, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
CN1316004A (en) 2001-10-03
AU5155299A (en) 2000-01-10
WO1999067402A2 (en) 1999-12-29
JP2002518054A (en) 2002-06-25
EP1090135A2 (en) 2001-04-11
CA2329690A1 (en) 1999-12-29

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