WO1999067401A3 - Non-food crop plant bioreactor - Google Patents

Non-food crop plant bioreactor Download PDF

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Publication number
WO1999067401A3
WO1999067401A3 PCT/CA1999/000577 CA9900577W WO9967401A3 WO 1999067401 A3 WO1999067401 A3 WO 1999067401A3 CA 9900577 W CA9900577 W CA 9900577W WO 9967401 A3 WO9967401 A3 WO 9967401A3
Authority
WO
WIPO (PCT)
Prior art keywords
crop plant
food crop
interest
csf
oral administration
Prior art date
Application number
PCT/CA1999/000577
Other languages
French (fr)
Other versions
WO1999067401A2 (en
Inventor
Jim Brandle
Peter L Davies
Kimberley D Kenward
Rima Menassa
Tony Jevnikar
Terry Delovitch
Original Assignee
Ca Minister Agriculture & Food
Univ Kingston
Robarts John P Res Inst
London Health Sciences Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Minister Agriculture & Food, Univ Kingston, Robarts John P Res Inst, London Health Sciences Center filed Critical Ca Minister Agriculture & Food
Priority to DE69941261T priority Critical patent/DE69941261D1/en
Priority to EP99926204A priority patent/EP1090133B8/en
Priority to AT99926204T priority patent/ATE439446T1/en
Priority to AU43550/99A priority patent/AU4355099A/en
Publication of WO1999067401A2 publication Critical patent/WO1999067401A2/en
Publication of WO1999067401A3 publication Critical patent/WO1999067401A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Fertilizers (AREA)

Abstract

A novel method for the production of transgenic proteins of interest suitable for oral administration is disclosed. The method involves the preparation of a protein of interest suitable for oral administration within a non-food crop plant comprising, transforming the non-food crop plant with a suitable vector containing a gene of interest and appropriate regulatory regions to ensure expression of the gene of interest within the non-food crop plant, such that the non-food crop plant is characterized as being non-toxic, non-addictive, palatable, and requiring minimal or no processing prior to oral administration. An example of a non-food crop plant is low lakaloid tobacco. Proteins of interest may include pharmaceutically active proteins such as growth regulators, insulin, interferons, interleukins, growth hormone, erythropoietin, G-CSF, GM-CSF, hPG-CSF, M-CSF, Factor VIII, Factor IX, tPA, antibodies, antigens and combinations and/or derivatives thereof.
PCT/CA1999/000577 1998-06-22 1999-06-21 Non-food crop plant bioreactor WO1999067401A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE69941261T DE69941261D1 (en) 1998-06-22 1999-06-21 PREPARATION OF IL-10 IN A PLANT BIOREACTOR FROM PLANTS NOT USED FOR NUTRITION
EP99926204A EP1090133B8 (en) 1998-06-22 1999-06-21 Production of il-10 in non-food crop plant bioreactor
AT99926204T ATE439446T1 (en) 1998-06-22 1999-06-21 PRODUCING IL-10 IN A PLANT BIOREACTOR FROM PLANTS NOT USED FOR FOOD
AU43550/99A AU4355099A (en) 1998-06-22 1999-06-21 Non-food crop plant bioreactor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10205098A 1998-06-22 1998-06-22
US09/102,050 1998-06-22

Publications (2)

Publication Number Publication Date
WO1999067401A2 WO1999067401A2 (en) 1999-12-29
WO1999067401A3 true WO1999067401A3 (en) 2000-03-23

Family

ID=22287857

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1999/000577 WO1999067401A2 (en) 1998-06-22 1999-06-21 Non-food crop plant bioreactor

Country Status (6)

Country Link
EP (1) EP1090133B8 (en)
AT (1) ATE439446T1 (en)
AU (1) AU4355099A (en)
DE (1) DE69941261D1 (en)
ES (1) ES2336861T3 (en)
WO (1) WO1999067401A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9220737B2 (en) 2003-04-27 2015-12-29 Protalix Ltd. Plant cell culture expressing human lysosomal proteins and uses thereof

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7361331B2 (en) * 1996-10-18 2008-04-22 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Plant bioreactors
GB2364705A (en) * 2000-07-14 2002-02-06 Icgeb Fusion protein expression in plastids
CA2448096A1 (en) 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes
CA2490781A1 (en) 2002-06-28 2004-01-08 University Of Guelph Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof
US7329798B2 (en) 2002-06-28 2008-02-12 University Of Guelph Harvest-inducible regulatory elements and methods of using same
AU2012241058B2 (en) * 2005-07-18 2014-04-17 Protalix Ltd. Mucosal or Enteral Administration of Biologically Active Macromolecules
US20110070201A1 (en) * 2005-07-18 2011-03-24 Protalix Ltd. Mucosal or enteral administration of biologically active macromolecules
US9194011B2 (en) 2009-11-17 2015-11-24 Protalix Ltd. Stabilized alpha-galactosidase and uses thereof
WO2012045334A1 (en) 2010-10-05 2012-04-12 Synthon Bv Biologically active il-10 fusion proteins
US9603906B2 (en) 2012-02-01 2017-03-28 Protalix Ltd. Inhalable liquid formulations of DNase I
CA2904035A1 (en) 2013-03-06 2014-09-12 Protalix Ltd. Tnf alpha inhibitor polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same
EP2964669B1 (en) 2013-03-06 2018-09-12 Protalix Ltd. Use of plant cells expressing a tnfalpha polypeptide inhibitor in therapy
US10000551B2 (en) 2014-09-11 2018-06-19 Protalix Ltd. Chimeric polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993003161A1 (en) * 1990-10-22 1993-02-18 Biosource Genetics Corporation Recombinant plant viral nucleic acids
CA2188220A1 (en) * 1996-10-18 1998-04-18 Kimberley Kenward Non-food crop plant bioreactor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993003161A1 (en) * 1990-10-22 1993-02-18 Biosource Genetics Corporation Recombinant plant viral nucleic acids
CA2188220A1 (en) * 1996-10-18 1998-04-18 Kimberley Kenward Non-food crop plant bioreactor

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SPURGEON D.: "Tobacco could be used to produce interleukin 10", BRITISH MEDICAL JOURNAL, vol. 319, no. 7203, 17 July 1999 (1999-07-17), pages 143 - 143, XP002127522 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9220737B2 (en) 2003-04-27 2015-12-29 Protalix Ltd. Plant cell culture expressing human lysosomal proteins and uses thereof

Also Published As

Publication number Publication date
ES2336861T3 (en) 2010-04-16
AU4355099A (en) 2000-01-10
EP1090133B1 (en) 2009-08-12
WO1999067401A2 (en) 1999-12-29
ATE439446T1 (en) 2009-08-15
EP1090133B8 (en) 2009-11-25
DE69941261D1 (en) 2009-09-24
EP1090133A2 (en) 2001-04-11

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