WO1999067389A3 - Cryptic regulatory elements obtained from plants - Google Patents

Cryptic regulatory elements obtained from plants Download PDF

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Publication number
WO1999067389A3
WO1999067389A3 PCT/CA1999/000578 CA9900578W WO9967389A3 WO 1999067389 A3 WO1999067389 A3 WO 1999067389A3 CA 9900578 W CA9900578 W CA 9900578W WO 9967389 A3 WO9967389 A3 WO 9967389A3
Authority
WO
WIPO (PCT)
Prior art keywords
promoter
gus
cryptic
regulatory elements
seed coat
Prior art date
Application number
PCT/CA1999/000578
Other languages
French (fr)
Other versions
WO1999067389A2 (en
Inventor
Brian Miki
Therese Ouellet
Jiro Hattori
Elizabeth Foster
Helene Labbe
Heller Teresa Martin
Lining Tian
Daniel Charles William Brown
Peijun Zhang
Keqiang Wu
Original Assignee
Ca Minister Agriculture & Food
Brian Miki
Therese Ouellet
Jiro Hattori
Elizabeth Foster
Helene Labbe
Heller Teresa Martin
Lining Tian
Daniel Charles William Brown
Peijun Zhang
Keqiang Wu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/441,597 external-priority patent/US5824863A/en
Priority claimed from CA002246892A external-priority patent/CA2246892A1/en
Application filed by Ca Minister Agriculture & Food, Brian Miki, Therese Ouellet, Jiro Hattori, Elizabeth Foster, Helene Labbe, Heller Teresa Martin, Lining Tian, Daniel Charles William Brown, Peijun Zhang, Keqiang Wu filed Critical Ca Minister Agriculture & Food
Priority to EP99926205A priority Critical patent/EP1088073A2/en
Priority to AU43551/99A priority patent/AU4355199A/en
Priority to CA002331842A priority patent/CA2331842C/en
Publication of WO1999067389A2 publication Critical patent/WO1999067389A2/en
Publication of WO1999067389A3 publication Critical patent/WO1999067389A3/en
Priority to US09/747,368 priority patent/US20010047091A1/en
Priority to US10/437,261 priority patent/US7303873B2/en
Priority to US10/866,529 priority patent/US20050055742A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

T-DNA tagging with a promoterless β-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.
PCT/CA1999/000578 1995-05-09 1999-06-22 Cryptic regulatory elements obtained from plants WO1999067389A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP99926205A EP1088073A2 (en) 1998-06-22 1999-06-22 Cryptic regulatory elements obtained from plants
AU43551/99A AU4355199A (en) 1998-06-22 1999-06-22 Cryptic regulatory elements obtained from plants
CA002331842A CA2331842C (en) 1998-06-22 1999-06-22 Cryptic regulatory elements obtained from plants
US09/747,368 US20010047091A1 (en) 1998-09-09 2000-12-22 Cryptic regulatory elements obtained from plants
US10/437,261 US7303873B2 (en) 1995-05-09 2003-05-13 Cryptic regulatory elements obtained from plants
US10/866,529 US20050055742A1 (en) 1996-02-01 2004-06-10 Plant regulatory element

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/441,597 US5824863A (en) 1995-05-09 1995-05-15 Seed coat-specific cryptic promoter in tobacco
US10231298A 1998-06-22 1998-06-22
US09/102,312 1998-06-22
CA002246892A CA2246892A1 (en) 1998-06-22 1998-09-09 Cryptic regulatory elements in plants
CA2,246,892 1998-09-09

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US45712399A Continuation 1995-05-09 1999-12-07

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/747,368 Continuation US20010047091A1 (en) 1995-05-09 2000-12-22 Cryptic regulatory elements obtained from plants

Publications (2)

Publication Number Publication Date
WO1999067389A2 WO1999067389A2 (en) 1999-12-29
WO1999067389A3 true WO1999067389A3 (en) 2000-03-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1999/000578 WO1999067389A2 (en) 1995-05-09 1999-06-22 Cryptic regulatory elements obtained from plants

Country Status (1)

Country Link
WO (1) WO1999067389A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004087878A2 (en) 2003-03-28 2004-10-14 Monsanto Technology, Llc Novel plant promoters for use in early seed development
BR122021003080B1 (en) 2009-08-31 2022-05-10 Basf Plant Science Company Gmbh Method of producing a plant promoter, method of producing a plant, construction, vector, transgenic microorganism and uses of neena and the recombinant construct or recombinant vector
CN112011566B (en) * 2009-08-31 2023-12-05 巴斯夫植物科学有限公司 Regulatory nucleic acid molecules for enhancing seed-specific gene expression in plants to promote enhanced polyunsaturated fatty acid synthesis
CN102482684B (en) 2009-08-31 2015-11-25 巴斯夫植物科学有限公司 For strengthening the regulatory nucleic acid molecules that in plant, constitutive gene is expressed
US9145562B2 (en) 2009-11-20 2015-09-29 Alberta Innovates—Technology Futures Variegation in plants

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0559603A2 (en) * 1992-01-09 1993-09-08 Sandoz Ltd. Brassica hsp 80 promoter
CA2149000A1 (en) * 1995-05-09 1996-11-10 Brian Miki A seed coat-specific cryptic promoter in tobacco
WO1997028268A1 (en) * 1996-02-01 1997-08-07 Her Majesty In Right Of Canada, Represented By The Minister Of Agriculture And Agri-Food Canada Promoter from tobacco
WO1999053067A2 (en) * 1998-04-13 1999-10-21 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Seed-coat promoters, genes and gene products

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0559603A2 (en) * 1992-01-09 1993-09-08 Sandoz Ltd. Brassica hsp 80 promoter
CA2149000A1 (en) * 1995-05-09 1996-11-10 Brian Miki A seed coat-specific cryptic promoter in tobacco
WO1997028268A1 (en) * 1996-02-01 1997-08-07 Her Majesty In Right Of Canada, Represented By The Minister Of Agriculture And Agri-Food Canada Promoter from tobacco
WO1999053067A2 (en) * 1998-04-13 1999-10-21 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Seed-coat promoters, genes and gene products

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
COCHEREL S ET AL: "A PROMOTER IDENTIFIED IN THE 3'-END OF THE AC TRANSPOSON CAN BE ACTIVATED BY CIS-ACTING ELEMENTS IN TRANSGENIC ARABIDOPSIS LINE", PLANT MOLECULAR BIOLOGY, (FEB 1996) VOL. 30, NO. 3, PP. 539-551. ISSN: 0167-4412., XP002124481 *
FOBERT P R ET AL: "T-DNA TAGGING OF A SEED COAT-SPECIFIC CRYPTIC PROMOTER IN TOBACCO", PLANT JOURNAL,GB,BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, vol. 6, no. 4, pages 567-577, XP002030412, ISSN: 0960-7412 *

Also Published As

Publication number Publication date
WO1999067389A2 (en) 1999-12-29

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