WO1999066074A8 - Vibrio vulnificus molecular probes, antibodies, and proteins - Google Patents

Vibrio vulnificus molecular probes, antibodies, and proteins

Info

Publication number
WO1999066074A8
WO1999066074A8 PCT/US1998/025729 US9825729W WO9966074A8 WO 1999066074 A8 WO1999066074 A8 WO 1999066074A8 US 9825729 W US9825729 W US 9825729W WO 9966074 A8 WO9966074 A8 WO 9966074A8
Authority
WO
WIPO (PCT)
Prior art keywords
wza
vulnificus
probes
protein
antibodies
Prior art date
Application number
PCT/US1998/025729
Other languages
French (fr)
Other versions
WO1999066074A1 (en
WO1999066074A9 (en
Inventor
Anita C Wright
Jan L Powell
J Glenn Morris Jr
Original Assignee
Univ Maryland Biotech Inst
Anita C Wright
Jan L Powell
J Glenn Morris Jr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland Biotech Inst, Anita C Wright, Jan L Powell, J Glenn Morris Jr filed Critical Univ Maryland Biotech Inst
Priority to AU50781/99A priority Critical patent/AU5078199A/en
Publication of WO1999066074A1 publication Critical patent/WO1999066074A1/en
Publication of WO1999066074A8 publication Critical patent/WO1999066074A8/en
Publication of WO1999066074A9 publication Critical patent/WO1999066074A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is directed to oligonucleotides used as amplification primers and assay probes for specific and sensitive for virulent strains of V. vulnificus. The target sequence of the probes and primers according to present invention is a capsular polysaccharide (CPS) transport gene (wza) of V. vulnificus. These probes can detect wza DNA or RNA in an unknown sample suspected to have pathogenic strains of V. vulnificus including human, animal, or environmental samples. The invention is also directed to in vitro-expressed protein from the cloned wza for production of polyclonal or monoclonal antibody that is specific for the wza gene product and will detect the V. vulnificus Wza protein in a sample comprising unknown protein.
PCT/US1998/025729 1998-06-19 1998-12-04 Vibrio vulnificus molecular probes, antibodies, and proteins WO1999066074A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU50781/99A AU5078199A (en) 1998-06-19 1998-12-04 $(vibrio vulnificus) molecular probes, antibodies, and proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
USPCT/US98/12467 1998-06-19
US9812467 1998-06-19

Publications (3)

Publication Number Publication Date
WO1999066074A1 WO1999066074A1 (en) 1999-12-23
WO1999066074A8 true WO1999066074A8 (en) 2000-02-10
WO1999066074A9 WO1999066074A9 (en) 2000-06-29

Family

ID=22267324

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/025729 WO1999066074A1 (en) 1998-06-19 1998-12-04 Vibrio vulnificus molecular probes, antibodies, and proteins

Country Status (2)

Country Link
AU (1) AU5078199A (en)
WO (1) WO1999066074A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112458090B (en) * 2020-12-04 2022-10-11 江苏海洋大学 DNAzyme for vibrio vulnificus specific recognition RNA cutting and application

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258284A (en) * 1991-01-22 1993-11-02 University Of Maryland, School Of Medicine Nucleic acid probes specific for pathogenic strains of vibrio vulnificus and method employing the same
US5426025A (en) * 1992-05-28 1995-06-20 Florida State University Species-specific DNA probes for vibrio vulnificus methods and kits

Also Published As

Publication number Publication date
AU5078199A (en) 2000-01-05
WO1999066074A1 (en) 1999-12-23
WO1999066074A9 (en) 2000-06-29

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