WO1999060152A8 - Process for sialylating glycoproteins - Google Patents

Process for sialylating glycoproteins

Info

Publication number
WO1999060152A8
WO1999060152A8 PCT/FI1999/000410 FI9900410W WO9960152A8 WO 1999060152 A8 WO1999060152 A8 WO 1999060152A8 FI 9900410 W FI9900410 W FI 9900410W WO 9960152 A8 WO9960152 A8 WO 9960152A8
Authority
WO
WIPO (PCT)
Prior art keywords
sialylating
glycoproteins
sialylating glycoproteins
hsp150δ
incubated
Prior art date
Application number
PCT/FI1999/000410
Other languages
French (fr)
Other versions
WO1999060152A1 (en
Inventor
Marja Makarow
Original Assignee
Marja Makarow
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Marja Makarow filed Critical Marja Makarow
Priority to EP99923639A priority Critical patent/EP1080221A1/en
Priority to AU40433/99A priority patent/AU4043399A/en
Publication of WO1999060152A1 publication Critical patent/WO1999060152A1/en
Publication of WO1999060152A8 publication Critical patent/WO1999060152A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to genetic engineering, and provides a novel process for sialylating glycoproteins. In the process said proteins are incubated with recombinant yeast cells expressing a sialyltransferase enzyme as a fusion protein with Hsp150Δ carrier in the porous cell wall.
PCT/FI1999/000410 1998-05-18 1999-05-12 Process for sialylating glycoproteins WO1999060152A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP99923639A EP1080221A1 (en) 1998-05-18 1999-05-12 Process for sialylating glycoproteins
AU40433/99A AU4043399A (en) 1998-05-18 1999-05-12 Process for sialylating glycoproteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI981099 1998-05-18
FI981099A FI104266B1 (en) 1998-05-18 1998-05-18 Method for Sialylation of Glycoproteins

Publications (2)

Publication Number Publication Date
WO1999060152A1 WO1999060152A1 (en) 1999-11-25
WO1999060152A8 true WO1999060152A8 (en) 2000-02-03

Family

ID=8551747

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI1999/000410 WO1999060152A1 (en) 1998-05-18 1999-05-12 Process for sialylating glycoproteins

Country Status (4)

Country Link
EP (1) EP1080221A1 (en)
AU (1) AU4043399A (en)
FI (1) FI104266B1 (en)
WO (1) WO1999060152A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002320485A (en) * 2000-11-21 2002-11-05 National Institute Of Advanced Industrial & Technology Expression vector for fusion gene and method for producing immobilized enzyme
CN108659095A (en) * 2018-05-18 2018-10-16 上海药明生物技术有限公司 A method of so that sialic acid content is stablized

Also Published As

Publication number Publication date
FI981099A0 (en) 1998-05-18
FI104266B (en) 1999-12-15
AU4043399A (en) 1999-12-06
WO1999060152A1 (en) 1999-11-25
EP1080221A1 (en) 2001-03-07
FI104266B1 (en) 1999-12-15

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