WO1999058653A3 - Verfahren zur erzeugung von pflanzen mit erhöhter toleranz gegen trockenheit und/oder pilzbefall und/oder erhöhte salzkonzentrationen und/oder extreme temperatur durch die expression plasmodesmen-lokalisierter proteine - Google Patents

Verfahren zur erzeugung von pflanzen mit erhöhter toleranz gegen trockenheit und/oder pilzbefall und/oder erhöhte salzkonzentrationen und/oder extreme temperatur durch die expression plasmodesmen-lokalisierter proteine Download PDF

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Publication number
WO1999058653A3
WO1999058653A3 PCT/EP1999/003291 EP9903291W WO9958653A3 WO 1999058653 A3 WO1999058653 A3 WO 1999058653A3 EP 9903291 W EP9903291 W EP 9903291W WO 9958653 A3 WO9958653 A3 WO 9958653A3
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WO
WIPO (PCT)
Prior art keywords
increased
plasmodesmata
salt concentrations
extreme temperature
tolerance against
Prior art date
Application number
PCT/EP1999/003291
Other languages
English (en)
French (fr)
Other versions
WO1999058653A2 (de
Inventor
Wolfgang Rohde
Dirk Pruefer
Eckhard Tacke
Peter Pasemann
Francesco Salamini
Original Assignee
Max Planck Gesellschaft
Wolfgang Rohde
Dirk Pruefer
Eckhard Tacke
Peter Pasemann
Francesco Salamini
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft, Wolfgang Rohde, Dirk Pruefer, Eckhard Tacke, Peter Pasemann, Francesco Salamini filed Critical Max Planck Gesellschaft
Priority to CA002328131A priority Critical patent/CA2328131A1/en
Priority to AU42609/99A priority patent/AU4260999A/en
Priority to EP99950352A priority patent/EP1078089A2/de
Publication of WO1999058653A2 publication Critical patent/WO1999058653A2/de
Publication of WO1999058653A3 publication Critical patent/WO1999058653A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Die Erfindung betrifft die Verwendung von Nukleinsäuren, die ein (Poly)peptid mit einer intrinsischen Affinität zu Plasmodesmen kodieren, zur Erzeugung von Pflanzen oder Teilen davon mit erhöhter Toleranz gegen Trockenheit und/oder Pilzinfektionen und/oder erhöhten Salzkonzentrationen und/oder extreme Temperatur (Hitze, Kälte) sowie entsprechende Verfahren. Vorteilhafterweise transfiziert man dabei eine Pflanze, ein Pflanzengewebe oder eine Pflanzenzelle mit der Nukleinsäure. Vorzugsweise kodiert die Nukleinsäure ein Virus-kodiertes Transportprotein, das in einer besonders bevorzugten Ausführungsform ein Derivat des pr17-Proteins mit einer hydrophilen N-terminalen Verlängerung ist.
PCT/EP1999/003291 1998-05-13 1999-05-12 Verfahren zur erzeugung von pflanzen mit erhöhter toleranz gegen trockenheit und/oder pilzbefall und/oder erhöhte salzkonzentrationen und/oder extreme temperatur durch die expression plasmodesmen-lokalisierter proteine WO1999058653A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002328131A CA2328131A1 (en) 1998-05-13 1999-05-12 Method for producing plants having an increased tolerance against drought and/or fungal attack and/or increased salt concentrations and/or extreme temperature by the expression ofplasmodesmata-localized proteins
AU42609/99A AU4260999A (en) 1998-05-13 1999-05-12 Method for producing plants having an increased tolerance against drought and/orfungal attack and/or increased salt concentrations and/or extreme temperature b y the expression of plasmodesmata-localized proteins
EP99950352A EP1078089A2 (de) 1998-05-13 1999-05-12 Verfahren zur erzeugung von pflanzen mit erhöhter toleranz gegen trockenheit und/oder pilzbefall und/oder erhöhte salzkonzentrationen und/oder extreme temperatur durch die expression plasmodesmen-lokalisierter proteine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98108726.5 1998-05-13
EP98108726 1998-05-13

Publications (2)

Publication Number Publication Date
WO1999058653A2 WO1999058653A2 (de) 1999-11-18
WO1999058653A3 true WO1999058653A3 (de) 2000-03-09

Family

ID=8231927

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/003291 WO1999058653A2 (de) 1998-05-13 1999-05-12 Verfahren zur erzeugung von pflanzen mit erhöhter toleranz gegen trockenheit und/oder pilzbefall und/oder erhöhte salzkonzentrationen und/oder extreme temperatur durch die expression plasmodesmen-lokalisierter proteine

Country Status (4)

Country Link
EP (1) EP1078089A2 (de)
AU (1) AU4260999A (de)
CA (1) CA2328131A1 (de)
WO (1) WO1999058653A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006137064A2 (en) * 2005-06-21 2006-12-28 Ramot At Tel Aviv University Ltd. Constructs and methods for generating plants exhibiting altered plasmodesmatal conductance
GB0613100D0 (en) * 2006-06-30 2006-08-09 Plant Bioscience Ltd Compositions and methods relating to cellular targeting

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997006669A1 (en) * 1995-08-15 1997-02-27 The Regents Of The University Of California Regulation of plant development and physiology through plasmodesmatal macromolecular transport of proteins and oligonucleotides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997006669A1 (en) * 1995-08-15 1997-02-27 The Regents Of The University Of California Regulation of plant development and physiology through plasmodesmatal macromolecular transport of proteins and oligonucleotides

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HERBERS, K., ET AL.: "expression of a luteoviral movement protein in transgenic plants leads to carbohydrate accumulation and reduced photosynthetic capacity in sourse leaves", THE PLANT JOURNAL, vol. 12, no. 5, 1997, pages 1045 - 1056, XP002125082 *
HERBERS, K., ET AL.: "systemic acquired resistance mediated by the ectopic expression of invertase: possible hexose sensing in the secretory pathway", THE PLANT CELL, vol. 8, May 1996 (1996-05-01), pages 793 - 803, XP002125083 *
SCHMITZ, J., ET AL.: "in situ localisation of the putative movement protein (pr17) from potato leafroll luteovirus (PLRV) in infected and transgenic potato plants", VIROLOGY, vol. 235, no. 2, September 1997 (1997-09-01), pages 311 - 322, XP002125084 *
SOKOLOVA, M., ET AL.: "the potato leafroll virus 17K movement protein is phosphorylated by a membrane-associated protein kinase from potato with biochemical features of protein kinase C", FEBS LETTERS, vol. 400, no. 2, January 1997 (1997-01-01), pages 201 - 205, XP002125086 *
TACKE, E., ET AL.: "mutational analysis of the nucleic acid-binding 17 KDa phophoprotein of Potato Leafroll Luteovirus identifies an amphipathic alpha-helix as the domain for protein/protein interactions", VIROLOGY, vol. 197, 1993, pages 274 - 282, XP002125085 *

Also Published As

Publication number Publication date
CA2328131A1 (en) 1999-11-18
AU4260999A (en) 1999-11-29
EP1078089A2 (de) 2001-02-28
WO1999058653A2 (de) 1999-11-18

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