WO1999055841A3 - Process for obtaining stem cells - Google Patents

Process for obtaining stem cells Download PDF

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Publication number
WO1999055841A3
WO1999055841A3 PCT/GB1999/001333 GB9901333W WO9955841A3 WO 1999055841 A3 WO1999055841 A3 WO 1999055841A3 GB 9901333 W GB9901333 W GB 9901333W WO 9955841 A3 WO9955841 A3 WO 9955841A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
stem cells
obtaining
oocyte
cells
Prior art date
Application number
PCT/GB1999/001333
Other languages
French (fr)
Other versions
WO1999055841A2 (en
Inventor
Austin Smith
Peter Scott Mountford
Megan Munsie
Original Assignee
Univ Edinburgh
Austin Smith
Peter Scott Mountford
Megan Munsie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Edinburgh, Austin Smith, Peter Scott Mountford, Megan Munsie filed Critical Univ Edinburgh
Priority to JP2000545985A priority Critical patent/JP2002512787A/en
Priority to CA002329975A priority patent/CA2329975A1/en
Priority to EP99918186A priority patent/EP1073719A2/en
Priority to AU36212/99A priority patent/AU3621299A/en
Priority to IL13932299A priority patent/IL139322A0/en
Publication of WO1999055841A2 publication Critical patent/WO1999055841A2/en
Publication of WO1999055841A3 publication Critical patent/WO1999055841A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of obtaining a stem cell is described, comprising obtaining a somatic cell, which cell contains a gene coding for a marker which is differentially expressed in (a) desired stem cells and (b) cells other than desired stem cells; obtaining an enucleated oocyte; transferring the nucleus of the somatic cell into the enucleated oocyte to form a transgenic oocyte; culturing the transgenic oocyte to produce a culture of cells derived from the transgenic oocyte and identifying a cell of the culture that expresses the marker, thus indicating that the nucleus of that cell has been reprogrammed. Also described are assays for factors that can reprogram nuclei and stem cells obtained directly or indirectly using the nuclear reprogramming methods described.
PCT/GB1999/001333 1998-04-29 1999-04-29 Process for obtaining stem cells WO1999055841A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2000545985A JP2002512787A (en) 1998-04-29 1999-04-29 How to get stem cells
CA002329975A CA2329975A1 (en) 1998-04-29 1999-04-29 Process for obtaining stem cells
EP99918186A EP1073719A2 (en) 1998-04-29 1999-04-29 Process for obtaining stem cells
AU36212/99A AU3621299A (en) 1998-04-29 1999-04-29 Process for obtaining stem cells
IL13932299A IL139322A0 (en) 1998-04-29 1999-04-29 Process for obtaining stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9809178.8 1998-04-29
GBGB9809178.8A GB9809178D0 (en) 1998-04-29 1998-04-29 Nuclear reprogramming of somatic cells

Publications (2)

Publication Number Publication Date
WO1999055841A2 WO1999055841A2 (en) 1999-11-04
WO1999055841A3 true WO1999055841A3 (en) 2000-01-06

Family

ID=10831203

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/001333 WO1999055841A2 (en) 1998-04-29 1999-04-29 Process for obtaining stem cells

Country Status (8)

Country Link
EP (1) EP1073719A2 (en)
JP (1) JP2002512787A (en)
AU (1) AU3621299A (en)
CA (1) CA2329975A1 (en)
GB (1) GB9809178D0 (en)
IL (1) IL139322A0 (en)
MX (1) MXPA00010473A (en)
WO (1) WO1999055841A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11851670B2 (en) 2008-06-13 2023-12-26 Whitehead Institute For Biomedical Research Nucleic acid constructs encoding reprogramming factors linked by self-cleaving peptides

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
JP2003503071A (en) * 1999-06-30 2003-01-28 アドバンスド セル テクノロジー、インコーポレイテッド Cytoplasmic transfer to dedifferentiated recipient cells
AU783162B2 (en) * 1999-09-07 2005-09-29 Advanced Cell Technology, Inc. Method for generating immune-compatible cells and tissues using nuclear transfer techniques
CN1377424A (en) * 1999-09-07 2002-10-30 先进细胞技术公司 Methods of reparing tandemly repeated DNA sequences and extending cell life-span using nuclear transfer
WO2001046401A1 (en) * 1999-12-20 2001-06-28 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Embryonic or stem-like cells produced by cross species nuclear transplantation
EP1395652A2 (en) * 2000-11-30 2004-03-10 Stemron, Inc. Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same
AU2002330465B2 (en) * 2001-09-21 2006-07-27 Kyoto University Screening method for a reprogramming agent, reprogramming agent screened by the method, method for using the reprogramming agent, method for differentiating an undifferentiated fusion cell, and production method of cells, tissues and organs.
CA2466203A1 (en) * 2001-11-06 2003-05-15 Shanghai Second Medical University Cross-species somatic cell nuclear transfer using rabbit oocyte
CN1683524A (en) * 2003-04-08 2005-10-19 东南大学 Method for preparing nuleus embryo stem cell from somatic cell transplanting technology
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US7964401B2 (en) 2004-02-19 2011-06-21 Kyoto University Screening method for somatic cell nuclear reprogramming substance affecting ECAT2 and ECAT3
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
JP2010523117A (en) 2007-04-07 2010-07-15 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ Somatic cell reprogramming
JP2008307007A (en) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
CN101855350B (en) 2008-05-02 2014-12-31 国立大学法人京都大学 Method of nuclear reprogramming

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024274A1 (en) * 1993-04-21 1994-10-27 The University Of Edinburgh Isolation, selection and propagation of animal transgenic stem cells
WO1995020042A1 (en) * 1994-01-25 1995-07-27 Ppl Therapeutics (Scotland) Limited Embryonic stem cell isolation
WO1997007669A1 (en) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer
WO1999019469A1 (en) * 1997-10-09 1999-04-22 Biotransplant, Inc. Porcine stem cells comprising a marker under an oct-4 promoter

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024274A1 (en) * 1993-04-21 1994-10-27 The University Of Edinburgh Isolation, selection and propagation of animal transgenic stem cells
WO1995020042A1 (en) * 1994-01-25 1995-07-27 Ppl Therapeutics (Scotland) Limited Embryonic stem cell isolation
WO1997007669A1 (en) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer
WO1999019469A1 (en) * 1997-10-09 1999-04-22 Biotransplant, Inc. Porcine stem cells comprising a marker under an oct-4 promoter

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J.A. THOMSON ET AL.: "EMBRYONIC STEN CELL LUNES DERIVED FROM HUMAN BLASTOCYSTS.", SCIENCE, vol. 282, 6 November 1998 (1998-11-06), LANCASTER, PA, US, pages 1145 - 1147, XP002121340 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11851670B2 (en) 2008-06-13 2023-12-26 Whitehead Institute For Biomedical Research Nucleic acid constructs encoding reprogramming factors linked by self-cleaving peptides

Also Published As

Publication number Publication date
AU3621299A (en) 1999-11-16
GB9809178D0 (en) 1998-07-01
EP1073719A2 (en) 2001-02-07
WO1999055841A2 (en) 1999-11-04
JP2002512787A (en) 2002-05-08
CA2329975A1 (en) 1999-11-04
MXPA00010473A (en) 2002-04-24
IL139322A0 (en) 2001-11-25

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