WO1999055833A3 - Lytobacter mycophilus, a novel bacterial genus with antifungal characteristics - Google Patents

Lytobacter mycophilus, a novel bacterial genus with antifungal characteristics Download PDF

Info

Publication number
WO1999055833A3
WO1999055833A3 PCT/US1999/009032 US9909032W WO9955833A3 WO 1999055833 A3 WO1999055833 A3 WO 1999055833A3 US 9909032 W US9909032 W US 9909032W WO 9955833 A3 WO9955833 A3 WO 9955833A3
Authority
WO
WIPO (PCT)
Prior art keywords
lytobacter
mycophilus
novel
bacterial genus
antifungal
Prior art date
Application number
PCT/US1999/009032
Other languages
French (fr)
Other versions
WO1999055833A2 (en
Inventor
Donald Y Kobayashi
Michael A Holtman
Original Assignee
Univ Rutgers
Donald Y Kobayashi
Michael A Holtman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rutgers, Donald Y Kobayashi, Michael A Holtman filed Critical Univ Rutgers
Priority to AU36660/99A priority Critical patent/AU3666099A/en
Publication of WO1999055833A2 publication Critical patent/WO1999055833A2/en
Publication of WO1999055833A3 publication Critical patent/WO1999055833A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01039—Glucan endo-1,3-beta-D-glucosidase (3.2.1.39)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Biomedical Technology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

A novel bacterial genus, Lytobacter, and its representative species, Lytobacter mycophilus, are disclosed. Lytobacter mycophilus strains are isolated from soil and display antifungal characteristics, specifically against Magnaporthe poae, due to the production of several lytic enzyme activities, including chitinase, β-1, 3-glucanase, lipase/esterase and protease. Chitinase and β-1, 3-glucanase enzymes produced by the novel bacterium are also disclosed. Bacteria from this novel genus and isolated antifungal components thereof are expected to be particularly useful for biological control of various fungal diseases of plants, particularly summer patch disease of turfgrass.
PCT/US1999/009032 1998-04-27 1999-04-27 Lytobacter mycophilus, a novel bacterial genus with antifungal characteristics WO1999055833A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU36660/99A AU3666099A (en) 1998-04-27 1999-04-27 (lytobacter mycophilus), a novel bacterial genus with antifungal characteristics

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8311998P 1998-04-27 1998-04-27
US60/083,119 1998-04-27

Publications (2)

Publication Number Publication Date
WO1999055833A2 WO1999055833A2 (en) 1999-11-04
WO1999055833A3 true WO1999055833A3 (en) 1999-12-16

Family

ID=22176302

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/009032 WO1999055833A2 (en) 1998-04-27 1999-04-27 Lytobacter mycophilus, a novel bacterial genus with antifungal characteristics

Country Status (2)

Country Link
AU (1) AU3666099A (en)
WO (1) WO1999055833A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007081027A1 (en) * 2006-01-16 2007-07-19 University Of Occupational And Environmental Health, Japan Method for analysis of bacterial flora in sample and use thereof
AU2017301012B2 (en) * 2016-07-19 2020-07-09 National Institute Of Plant Genome Research Novel protein against fungal pathogens
CN111979143B (en) * 2020-08-04 2022-04-29 安徽科技学院 Lysobacter strain and production application thereof
CN114015612B (en) * 2021-11-30 2023-08-01 山东省食品发酵工业研究设计院 Xanthomonas leucovorin and application thereof in fermentation production of non-pigmented xanthan gum

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK, Accession Number U89956, HOLTMAN M.A. et al., "N4-7 Gen. Nov. Sp. Nov.: Gram-Negative Biocontrol Bacterium Identified on the Basis of Morphological, Biochemical, 16S rDNA Sequence, Fatty Acid and Serological Characteristics. 1574bp DNA Sequence Comparison", 18 March 1997. *
HOLTMAN M. A., ET AL.: "CLONING OF SS-1,3-GLUCANASES FROM THE BACTERIAL BIOCONTROL AGENT, N4-7.", PHYTOPATHOLOGY, AMERICAN PHYTOPATHOLOGICAL SOCIETY, US, vol. 85., no. 12., 1 January 1995 (1995-01-01), US, pages 1557., XP002923403, ISSN: 0031-949X *
KOBAYASHI D.Y., EL-BARRAD N.E.H.: "SELECTION OF BACTERIAL ANTAGONISTS USING ENRICHMENT CULTURES FOR THE CONTROL OF SUMMER PATCH DISEASE IN KENTUCKY BLUEGRASS.", CURRENT MICROBIOLOGY, SPRINGER, BOSTON, vol. 32., 1 February 1996 (1996-02-01), Boston, pages 106 - 110., XP002923404, ISSN: 0343-8651, DOI: 10.1007/s002849900019 *
THOMPSON D.C., CLARKE B.B., KOBAYASHI D.Y.: "EVALUATION OF BACTERIAL ANTAGONISTS FOR REDUCTION OF SUMMER PATCH SYMPTOMS IN KENTUCKY BLEUGRASS.", PLANT DISEASE, THE AMERICAN PHYTOPATHOLOGICAL SOCIETY, US, vol. 80., no. 08., 1 August 1996 (1996-08-01), US, pages 856 - 862., XP002923405, ISSN: 0191-2917 *

Also Published As

Publication number Publication date
WO1999055833A2 (en) 1999-11-04
AU3666099A (en) 1999-11-16

Similar Documents

Publication Publication Date Title
Jain et al. Microbial consortium–mediated reprogramming of defence network in pea to enhance tolerance against Sclerotinia sclerotiorum
Wu et al. Suppression of Fusarium wilt of watermelon by a bio-organic fertilizer containing combinations of antagonistic microorganisms
Kamensky et al. Soil-borne strain IC14 of Serratia plymuthica with multiple mechanisms of antifungal activity provides biocontrol of Botrytis cinerea and Sclerotinia sclerotiorum diseases
Castoria et al. Aureobasidium pullulans (LS-30) an antagonist of postharvest pathogens of fruits: study on its modes of action
Binod et al. Production and purification of extracellular chitinases from Penicillium aculeatum NRRL 2129 under solid-state fermentation
Solanki et al. Plant defense activation and management of tomato root rot by a chitin-fortified Trichoderma/Hypocrea formulation
Alamri et al. In vitro and in vivo biocontrol of soil-borne phytopathogenic fungi by certain bioagents and their possible mode of action
Li et al. Mechanism of in vitro antagonism of phytopathogenic Scelrotium rolfsii by actinomycetes
Helistö et al. Lytic enzyme complex of an antagonistic Bacillus sp. Xb: isolation and purification of components
Thakaew et al. Inhibitory activity of Bacillus subtilis BCC 6327 metabolites against growth of aflatoxigenic fungi isolated from bird chili powder
Dos Santos et al. Marine-derived fungus Aspergillus cf. tubingensis LAMAI 31: a new genetic resource for xylanase production
Duncan et al. Screening fungi isolated from historic Discovery Hut on Ross Island, Antarctica for cellulose degradation
Ekundayo et al. Production, partial purification and optimization of a chitinase produced from Trichoderma viride, an isolate of maize cob
Innocenti et al. Efficacy of microorganisms antagonistic to Rhizoctonia cerealis and their cell wall degrading enzymatic activities
Nwodo-Chinedu et al. Isolation of cellulolytic microfungi involved in wood-waste decomposition: prospects for enzymatic hydrolysis of cellulosic wastes
Kumar et al. Evaluation of extracellular lytic enzymes from indigenous Bacillus isolates
Bedade et al. Fermentative production of extracellular amylase from novel amylase producer, Tuber maculatum mycelium, and its characterization
WO1999055833A3 (en) Lytobacter mycophilus, a novel bacterial genus with antifungal characteristics
Verma et al. Influence of soil reaction on diversity and antifungal activity of fluorescent pseudomonads in crop rhizospheres
Thiep et al. Reserch and development of enzymatic producing fungi as biofertilizer for tea and Arabica coffee production in northern Vietnam.
Gomathinayagam et al. The biological control of paddy disease brown spot (Bipolaris oryzae) by using Trichoderma viride in vitro condition
Sreedevi et al. Production and optimization of chitinase by Trichoderma harzianum for control of the phytopathogenic fungus M. phaseolina.
KR102125358B1 (en) Novel Aspergillus oryzae IF18-2 with high saccharogenic power and high liquerying activity and drying method thereof
Varma et al. Evaluation of endophytic bacteria for plant growth promotion and pathogen suppression traits in Saccharum officinarum
Terna et al. Growth Suppression of some common post harvest rot fungi by culture filtrates of a soil isolate of Trichoderma viride

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 09673401

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: KR

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase