WO1999049831A3 - Amplified fluorescent materials and uses thereof - Google Patents

Amplified fluorescent materials and uses thereof Download PDF

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Publication number
WO1999049831A3
WO1999049831A3 PCT/US1999/000203 US9900203W WO9949831A3 WO 1999049831 A3 WO1999049831 A3 WO 1999049831A3 US 9900203 W US9900203 W US 9900203W WO 9949831 A3 WO9949831 A3 WO 9949831A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescent
molecules
amplifier
molecule
moieties
Prior art date
Application number
PCT/US1999/000203
Other languages
French (fr)
Other versions
WO1999049831A2 (en
Inventor
Vladimir V Martin
Alexander Weis
Original Assignee
Vladimir V Martin
Alexander Weis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vladimir V Martin, Alexander Weis filed Critical Vladimir V Martin
Priority to AU23115/99A priority Critical patent/AU2311599A/en
Publication of WO1999049831A2 publication Critical patent/WO1999049831A2/en
Publication of WO1999049831A3 publication Critical patent/WO1999049831A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Materials Engineering (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Compositions of fluorescent amplifier molecules are provided, as well as synthetic methods for their preparation. The procedures provide for the preparation of gram quantities of fluorescent molecules from available precursors. The fluorescent amplifier molecules include a multi-dimensional, rigid core (C), to which at least two fluorescent moieties are attached by way of a linker (L). Amplifier molecules that further include a 'targeting' molecule (a molecule that functions to direct the fluorescent amplifier to a particular binding site) are also presented, the targeting molecule being attached to the rigid, multi-dimensional core. Signal amplification without steric restrictions of fluorophore mobility is provided, hence reducing and/or avoiding interactions between moieties as well as fluorescence quenching. The advantage of the derivatives of the present invention, in addition to other things, over conventional fluorescent labels is due to the ability to amplify signal upon accumulation of the fluorescent moieties. The new amplified molecules are further disclosed to have the capability to quantitate the fluorescence signal. the compositions may also be employed as nucleic acid fluorescent -in-situ-hybridization probes.
PCT/US1999/000203 1998-03-28 1999-01-05 Amplified fluorescent materials and uses thereof WO1999049831A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU23115/99A AU2311599A (en) 1998-03-28 1999-01-05 Amplified fluorescent materials and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7984998P 1998-03-28 1998-03-28
US60/079,849 1998-03-28

Publications (2)

Publication Number Publication Date
WO1999049831A2 WO1999049831A2 (en) 1999-10-07
WO1999049831A3 true WO1999049831A3 (en) 2000-01-27

Family

ID=22153193

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/000203 WO1999049831A2 (en) 1998-03-28 1999-01-05 Amplified fluorescent materials and uses thereof

Country Status (2)

Country Link
AU (1) AU2311599A (en)
WO (1) WO1999049831A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7354707B2 (en) * 2000-06-27 2008-04-08 National Institute Of Advanced Industrial Science And Technology Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method
JP4649752B2 (en) * 2001-03-21 2011-03-16 株式会社デンソー Adamantane derivative compound and electroluminescent device using the same
GB0119001D0 (en) * 2001-08-03 2001-09-26 Amersham Pharm Biotech Uk Ltd Use of dendrimers and poly-branched molecules to enhance signal in fluorescent assay systems
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
CN113122229B (en) * 2019-12-31 2023-04-18 Tcl科技集团股份有限公司 Quantum dot material, preparation method thereof, thin film and quantum dot light-emitting diode

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990007511A1 (en) * 1988-12-27 1990-07-12 Board Of Governors Of Wayne State University Enhanced chemiluminescence from 1,2-dioxetanes through energy transfer to tethered fluorescers
WO1994029329A1 (en) * 1993-06-10 1994-12-22 Abbott Laboratories Haptens, tracers, immunogens and antibodies for 3-phenyl-1-adamantaneacetic acids
US5407834A (en) * 1986-05-21 1995-04-18 Abbott Laboratories Phencyclidine and phencyclidine metabolites assay, tracers, immunogens, antibodies and reagent kit
JPH08271430A (en) * 1995-03-28 1996-10-18 Aisin Seiki Co Ltd Novel fluorescent dye

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5407834A (en) * 1986-05-21 1995-04-18 Abbott Laboratories Phencyclidine and phencyclidine metabolites assay, tracers, immunogens, antibodies and reagent kit
US5616729A (en) * 1986-07-17 1997-04-01 Board Of Governors Of Wayne State University Enhanced chemiluminescence from 1,2-dioxetanes through energy transfer to tethered fluorescers
WO1990007511A1 (en) * 1988-12-27 1990-07-12 Board Of Governors Of Wayne State University Enhanced chemiluminescence from 1,2-dioxetanes through energy transfer to tethered fluorescers
WO1994029329A1 (en) * 1993-06-10 1994-12-22 Abbott Laboratories Haptens, tracers, immunogens and antibodies for 3-phenyl-1-adamantaneacetic acids
JPH08271430A (en) * 1995-03-28 1996-10-18 Aisin Seiki Co Ltd Novel fluorescent dye

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ADAM W., BRONSTEIN I., TROFIMOV A.V.: "SOLVATOCHROMIC EFFECTS ON THE ELECTRON EXCHANGE CHEMILUMINESCENCE (CIEEL) OF SPIROADAMANTYL-SUBSTITUTED DIOXETANES AND THE FLUORESCENCE OF RELEVANT OXYANIONS.", JOURNAL OF PHYSICAL CHEMISTRY. A, MOLECULES, SPECTROSCOPY,KINETICS, ENVIRONMENT AND GENERAL THEORY, WASHINGTON, DC, US, vol. 102., 9 July 1998 (1998-07-09), US, pages 5406 - 5414., XP002922465, ISSN: 1089-5639, DOI: 10.1021/jp973175c *
CONGER B. M., ET AL.: "FLUORESCENCE OF PYRENYL AND CARBAZOLYL DERIVATIVES IN LIQUID SOLUTION AND SOLID FILM.", JOURNAL OF PHYSICAL CHEMISTRY. A, MOLECULES, SPECTROSCOPY,KINETICS, ENVIRONMENT AND GENERAL THEORY, WASHINGTON, DC, US, vol. 102., no. 46., 12 November 1998 (1998-11-12), US, pages 9213 - 9218., XP002922464, ISSN: 1089-5639, DOI: 10.1021/jp983020k *
IKEDA H., ET AL.: "NMR STUDIES OF CONFORMATIONS OF N-DANSYL-L-LEUCINE-APPENDED AND N-DANSYL-D-LEUCINE-APPENDED BETA-CYCLODEXTRIN AS FLUORESCENT INDICATORS FOR MOLECULAR RECOGNITION.", THE JOURNAL OF ORGANIC CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 62., 7 August 1997 (1997-08-07), US, pages 1411 - 1418., XP002922466, ISSN: 0022-3263, DOI: 10.1021/jo960425x *
MARTIN V.V., ALFERIEV I.S., WEIS A.L.: "AMPLIFIED FLUORESCENT MOLECULAR PROBES BASED ON 1,3,5,7-TETRASUBSTITUTED ADAMANTANE.", TETRAHEDRON LETTERS, PERGAMON, GB, vol. 40., no. 02., 8 January 1999 (1999-01-08), GB, pages 223 - 226., XP002922463, ISSN: 0040-4039, DOI: 10.1016/S0040-4039(98)02280-1 *
MASTRANGELO J.C., CONGER B.M., CHEN S.H.: "NOVEL GLASS-FORMING ORGANIC MATERIALS. 2. STRUCTURE AND FLUORESCENCE OF PYRENE- AND CARBAZOLE-CONTAINING CYCLOHEXANE, BICYCLOOCTENE, AND ADAMANTANE.", CHEMISTRY OF MATERIALS, AMERICAN CHEMICAL SOCIETY, US, vol. 09., 1 January 1997 (1997-01-01), US, pages 227 - 232., XP002922467, ISSN: 0897-4756, DOI: 10.1021/cm960297t *
NEWKOME G.R., ET AL.: "CASCADE POLYMERS: SYNTHESIS AND CHARACTERIZATION OF FOUR-DIRECTIONAL SPHERICAL DENDRITIC MACROMOLECULES BASED ON ADAMANTANE.", THE JOURNAL OF ORGANIC CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 57., 1 January 1992 (1992-01-01), US, pages 358 - 362., XP002922462, ISSN: 0022-3263, DOI: 10.1021/jo00027a061 *

Also Published As

Publication number Publication date
WO1999049831A2 (en) 1999-10-07
AU2311599A (en) 1999-10-18

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