WO1999049292A3 - Reagents and methods for protein microsequencing - Google Patents

Reagents and methods for protein microsequencing Download PDF

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Publication number
WO1999049292A3
WO1999049292A3 PCT/US1999/004337 US9904337W WO9949292A3 WO 1999049292 A3 WO1999049292 A3 WO 1999049292A3 US 9904337 W US9904337 W US 9904337W WO 9949292 A3 WO9949292 A3 WO 9949292A3
Authority
WO
WIPO (PCT)
Prior art keywords
reagents
protein microsequencing
methods
femtomole
microsequencing
Prior art date
Application number
PCT/US1999/004337
Other languages
French (fr)
Other versions
WO1999049292A2 (en
WO1999049292A9 (en
Inventor
Andrzej Wilk
Andrzej K Drukier
Original Assignee
Biotraces Inc
Andrzej Wilk
Andrzej K Drukier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotraces Inc, Andrzej Wilk, Andrzej K Drukier filed Critical Biotraces Inc
Priority to EP99931744A priority Critical patent/EP1060377A4/en
Priority to AU48177/99A priority patent/AU4817799A/en
Publication of WO1999049292A2 publication Critical patent/WO1999049292A2/en
Publication of WO1999049292A3 publication Critical patent/WO1999049292A3/en
Publication of WO1999049292A9 publication Critical patent/WO1999049292A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6824—Sequencing of polypeptides involving N-terminal degradation, e.g. Edman degradation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention provides methods of peptide and protein microsequencing using radiolabeled reagents to perform peptide and protein microsequencing at levels lower than 10 femtomole, preferably at a level lower than 1 femtomole.
PCT/US1999/004337 1998-03-02 1999-02-26 Reagents and methods for protein microsequencing WO1999049292A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP99931744A EP1060377A4 (en) 1998-03-02 1999-02-26 Reagents and methods for protein microsequencing
AU48177/99A AU4817799A (en) 1998-03-02 1999-02-26 Reagents and methods for protein microsequencing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7647998P 1998-03-02 1998-03-02
US60/076,479 1998-03-02

Publications (3)

Publication Number Publication Date
WO1999049292A2 WO1999049292A2 (en) 1999-09-30
WO1999049292A3 true WO1999049292A3 (en) 1999-11-11
WO1999049292A9 WO1999049292A9 (en) 1999-12-09

Family

ID=22132304

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/004337 WO1999049292A2 (en) 1998-03-02 1999-02-26 Reagents and methods for protein microsequencing

Country Status (3)

Country Link
EP (1) EP1060377A4 (en)
AU (1) AU4817799A (en)
WO (1) WO1999049292A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4865994A (en) * 1985-05-17 1989-09-12 Seiko Instruments & Electronics Ltd. Detection method for amino acid derivatives
US5270213A (en) * 1991-06-21 1993-12-14 Porton Instruments, Inc. Protein sequencing

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5854084A (en) * 1996-07-12 1998-12-29 Biotraces, Inc. Enhanced chromatography using multiphoton detection

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4865994A (en) * 1985-05-17 1989-09-12 Seiko Instruments & Electronics Ltd. Detection method for amino acid derivatives
US5270213A (en) * 1991-06-21 1993-12-14 Porton Instruments, Inc. Protein sequencing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1060377A4 *

Also Published As

Publication number Publication date
WO1999049292A2 (en) 1999-09-30
EP1060377A2 (en) 2000-12-20
EP1060377A4 (en) 2002-06-12
AU4817799A (en) 1999-10-18
WO1999049292A9 (en) 1999-12-09

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