WO1999049063B1 - Protein expression in floral cells - Google Patents

Protein expression in floral cells

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Publication number
WO1999049063B1
WO1999049063B1 PCT/CA1999/000237 CA9900237W WO9949063B1 WO 1999049063 B1 WO1999049063 B1 WO 1999049063B1 CA 9900237 W CA9900237 W CA 9900237W WO 9949063 B1 WO9949063 B1 WO 9949063B1
Authority
WO
WIPO (PCT)
Prior art keywords
protein
peptide
cell
fusion protein
fragment
Prior art date
Application number
PCT/CA1999/000237
Other languages
French (fr)
Other versions
WO1999049063A1 (en
Inventor
Laurian S Robert
Stephen Gleddie
Original Assignee
Ca Minister Agriculture & Food
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Minister Agriculture & Food filed Critical Ca Minister Agriculture & Food
Priority to AU28229/99A priority Critical patent/AU2822999A/en
Priority to CA002325570A priority patent/CA2325570A1/en
Priority to EP99908716A priority patent/EP1062352A2/en
Publication of WO1999049063A1 publication Critical patent/WO1999049063A1/en
Publication of WO1999049063B1 publication Critical patent/WO1999049063B1/en

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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/641—Cysteine endopeptidases (3.4.22)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

This invention is directed to a method for the expression of a gene of interest, or a chimeric or modified gene allowing the localization of a protein, protein fusion, peptide or fragment of interest within the extracellular domain of a floral cell. This method comprises preparing a construct comprising a promoter sequence capable of expressing a gene encoding the protein, protein fusion, peptide, or fragment of interest, within the floral cell; a translated sequence of the protein, protein fusion, peptide, or fragment of interest, which is localized within the extracellular domain of a floral cell; a gene that encodes the protein, protein fusion, peptide, or fragment of interest; and a terminator sequence, and transforming a plant. Plants transformed with such a construct are characterized as having a protein, fragment thereof, or peptide of interest on the surface of a floral cell. Such localized proteins or peptides may be used for the purposes of peptide display, mediating plant sterility, modifying pollen-pistil interactions, altering pollen for insect consumption etc.

Claims

- 65 -AMENDED CLAIMS[received by the International Bureau on 3 November 1999 (03.11.99); original claims 1-40 replaced by amended claims 1-46 (4 pages)]
1. A method for modifying the extracellular compartment of a pollen grain or an anther cell of a plant, said method comprising: expressing a construct comprising a gene of interest within said pollen grain or anther cell, said gene of interest encoding a protein, fusion protein or peptide, or a fragment of said protein, fusion protein or peptide, said protein, fusion protein or peptide, or a fragment of said protein, fusion protein or peptide capable of modifying the composition ofthe extracellular compartment of said pollen grain or anther cell and altering either the function, use, or development of said pollen grain or anther cell, or modifying the interaction of said pollen grain or anther cell with a cell, said protein or other proteins, or a chemical.
2. The method of claim 1 wherein said gene of interest is native to said plant.
3. The method of claim 1 wherein said gene of interest is non-native to said plant.
4. The method of claim 1 wherein said construct is a chimeric gene construct.
5. The method of claim 1 wherein said protein, fusion protein or peptide, or a fragment of said protein, fusion protein or peptide is released into a locule of an anther thereby associating with or modifying said extracellular compartment of said pollen grain.
6. The method of claim 1 wherein said construct comprises a translated sequence capable of directing the extracellular localization of said protein, fusion protein or peptide, or a fragment of said protein, fusion protein or peptide on said pollen grain.
7. The method of claim 6, wherein said translated sequence is selected from the group consisting of a signal peptide, a hydrophobic domain, or a combination thereof.
8. The method of claim 6 wherein said translated sequence is a protein, or fragment thereof, known to be targeted to the extracellular compartment of said pollen grain or anther cell.
9. The method of claim 8 wherein said protein or fragment thereof is an oleosin-like protein.
10 The method of claim 8 wherein said protein or fragment thereof is selected from the group consisting of Sta 41-2, Sta 41-9, or Sta 44.
11. A method for obtaining the localization of a protein, fusion protein, or peptide of interest, or a fragment ofthe protein, fusion protein, or peptide, within or on the extracellular compartment of a pollen grain or an anther cell, comprising:
i) preparing a construct comprising: a) a promoter sequence capable of expressing a gene encoding said protein, fusion protein, or peptide of interest, or a fragment ofthe protein, fusion protein, or peptide, within said pollen grain or anther cell; - 66 -
b) a gene that encodes said protein, fusion protein, or peptide of interest, or a fragment ofthe protein, fusion protein, or peptide; and c) a translated sequence capable of directing the extracellular localization of said protein, fusion protein, or peptide of interest, or a fragment of the protein, fusion protein, or peptide. on said pollen grain or anther cell; d) a terminator sequence; and ii) transforming a plant with said construct.
12. The method of claim 1 1, wherein the translated sequence of step c) is selected from the group consisting of a signal peptide, a hydrophobic domain, or a combination thereof.
13. The method of claim 11 , wherein the translated sequence of step c) is a protein, or fragment thereof, known to be targeted to the extracellular compartment of said pollen grain or anther cell.
14. The method of claim 13 wherein said protein or fragment thereof is an oleosin-like protein.
15. The method of claim 13 wherein said protein or fragment thereof is selected from the group consisting of Sta 41-2, Sta 41-9, or Sta 44.
16. A method of chemically linking a protein or peptide of interest to the pollen coat comprising: a) activating pollen grains with a desired reagent for conjugation; b) adding the protein of interest
17. A microspore or a pollen, or combination thereof prepared by the method of claim 16.
18. A microspore or a pollen, or combination thereof, prepared using the method of claim 1.
19. A vector comprising : a) a promoter sequence capable of expressing a gene encoding a protein, fusion protein, or peptide of interest, or a fragment ofthe protein, fusion protein, or peptide, within a pollen grain or an anther cell; b) a gene that encodes the protein, fusion protein, or peptide of interest, or a fragment ofthe protein, fusion protein, or peptide; c) a translated sequence capable of directing the extracellular localization of the protein, fusion protein, or peptide of interest, or a fragment ofthe protein, fusion protein, or peptide, on said pollen grain or anther cell; and d) a terminator sequence.
20. A transgenic plant cell comprising the vector of claim 19.
21. A transgenic plant comprising the vector of claim 19 - 67 -
22. A seed obtained from the transgenic plant of claim 19.
23. A pollen characterized in that the extracellular compartment of the pollen is modified, said extracellular compartment comprising a protein, a fusion protein, or a peptide of interest, or a fragment of the protein, fusion protein, or peptide.
24. A transgenic plant comprising the pollen as claimed in claim 23.
25. A seed obtained from the transgenic plant of claim 24.
26. The method of claim 1 , wherein the extracellular compartment comprises either the tryphine, exine, nexine, sexine, intine, or a combination thereof.
27 The method of claim 1, wherein the anther cell is a tapetal cell.
28. A method of modifying pollen-pistil interaction or function comprising, producing a microspore, pollen, or combination thereof, within a plant using the method of claim 1 , so that the microspore, pollen, or combination thereof, comprises a modified extracellular domain.
29. The method of claim 28, wherein the pollen-pistil interaction or function being modified mediates, produces or prevents self compatibility, self incompatibility, out-crossing, in-crossing or a combination thereof.
30. The method of claim 1 wherein the protein, fusion protein, or peptide of interest, or the fragment of a protein, fusion protein, or peptide is localized on the surface of pollen for the purpose of peptide display.
31. The method of claim 1 wherein the protein, fusion protein, or peptide of interest, or a fragment ofthe protein, fusion protein, or peptide, is localized on the surface of pollen and it is an antibody or antigen.
32. The method of claim 1 wherein the protein, fusion protein, or peptide of interest, or a fragment ofthe protein, fusion protein, or peptide, is localized on the surface ofthe pollen grain and it is effective in controlling insect growth, behaviour, feeding, development, or reproduction, or a combination thereof.
33 A method for modifying the extracellular compartment of a pistil cell of a plant, said method comprising: expressing a construct comprising a gene of interest within said pistil cell, said gene of interest encoding a fusion protein or peptide, or a fragment of said fusion protein or peptide, said fusion protein or peptide, or a fragment of said fusion protein or peptide capable of modifying the composition ofthe extracellular compartment of said pistil cell and altering either the function, use, or development of said pistil cell, or modifying the interaction of said pistil cell with a cell protein or chemical.
34. The method of claim 33 wherein said gene of interest is native to said plant.
35. The method of claim 33 wherein said gene of interest is non-native to said plant.
36. The method of claim 33 wherein said construct is a chimeric gene construct.
37. The method of claim 33 wherein said pistil cell is a stigma cell, and said construct comprises a translated sequence capable of directing the extracellular localization of said fusion protein or peptide, or a fragment of said fusion protein or peptide on said stigma cell.
38. The method of claim 37 wherein said gene of interest is selected from the group consisting of SLGWS, or GPIS363.
39. The method of claim 33, wherein said extracellular compartment comprises the cuticle, cell wall, pellicle, transmitting tract, or extracellular secretions, or combination thereof.
40. A pistil cell prepared using the method of claim 33.
41. A transgenic plant comprising the pistil cell as claimed in claim 40.
42. A seed obtained from the transgenic plant of claim 41.
43. A stigma cell characterized in that the extracellular compartment of said cell is modified, said extracellular compartment comprising a fusion protein, or a fragment thereof, encoded by a chimeric gene construct.
44. A transgenic plant comprising the pistil cell as claimed in claim 43.
45. A seed obtained from the transgenic plant of claim 44.
46. A method for modifying the extracellular compartment of a pistil cell of a plant, said method comprising: expressing a construct comprising a gene of interest within said pistil cell, said gene of interest encoding a protein, fusion protein or peptide, or a fragment of said protein, fusion protein or peptide, said protein, fusion protein or peptide, or a fragment of said protein, fusion protein or peptide capable of modifying the composition ofthe extracellular compartment of said pistil cell and altering either the function, use, or development of said pistil cell, or modifying the interaction of said pistil cell with a modified cell, a protein or a chemical.
PCT/CA1999/000237 1998-03-20 1999-03-19 Protein expression in floral cells WO1999049063A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU28229/99A AU2822999A (en) 1998-03-20 1999-03-19 Protein expression in floral cells
CA002325570A CA2325570A1 (en) 1998-03-20 1999-03-19 Protein expression in floral cells
EP99908716A EP1062352A2 (en) 1998-03-20 1999-03-19 Protein expression in floral cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7872898P 1998-03-20 1998-03-20
US60/078,728 1998-03-20

Publications (2)

Publication Number Publication Date
WO1999049063A1 WO1999049063A1 (en) 1999-09-30
WO1999049063B1 true WO1999049063B1 (en) 2000-01-06

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EP (1) EP1062352A2 (en)
AU (1) AU2822999A (en)
CA (1) CA2325570A1 (en)
WO (1) WO1999049063A1 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7608270B2 (en) 2003-06-27 2009-10-27 University Of Hull Dosage form
BRPI0305197B8 (en) * 2003-11-13 2021-05-25 Fundacao Oswaldo Cruz pharmaceutical product and process for its production
WO2007012857A1 (en) 2005-07-28 2007-02-01 University Of Hull Topical formulations containing sporopollenin
GB0812513D0 (en) 2008-07-09 2008-08-13 Univ Hull Delivery vehicle
US10604763B2 (en) 2014-08-06 2020-03-31 The Texas A & M University System Processes and products for enhanced biological product

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ217916A (en) * 1985-10-29 1990-03-27 Lubrizol Genetics Inc Recombinant vector including a self-incompatibility gametophytic plant gene and relevant dna
US5053331A (en) * 1986-04-21 1991-10-01 Lubrizol Genetics, Inc. Self-incompatibility gene
GB8626878D0 (en) * 1986-11-11 1986-12-10 Ici Plc Dna
US5275819A (en) * 1989-02-06 1994-01-04 Amer Particle Technologies Inc. Drug loaded pollen grains with an outer coating for pulsed delivery
EP0436467A3 (en) * 1989-12-29 1992-10-21 Ciba-Geigy Ag Expression of s-locus specific glycoprotein gene in transgenic plants
US5650554A (en) * 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
WO1993018149A1 (en) * 1992-03-03 1993-09-16 Pioneer Hi-Bred International, Inc. Self-incompatibility alleles of brassica
AU5298593A (en) * 1992-10-08 1994-05-09 Pioneer Hi-Bred International, Inc. S-locus receptor kinase gene in a self-incompatible (brassica) napus line
US5585543A (en) * 1994-02-09 1996-12-17 The Penn State Research Foundation Alteration of plant self-compatibility using genetic manipulation of the S-genes

Also Published As

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EP1062352A2 (en) 2000-12-27
WO1999049063A1 (en) 1999-09-30
AU2822999A (en) 1999-10-18
CA2325570A1 (en) 1999-09-30

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